A&P 1 - Lab #2

Microscopy

Cell Structure

Mitosis

Rotatable Head

Ocular Lens

Objective Lens

Arm

Illuminator

Condenser

Stage

Base

Course Adjustment Knob

Fine Adjustment Knob

Mechanical Stage

Mechanical Stage Control

Magnification

• Total magnification is achieved by multiplying the power of the ocular lens by the power of the objective lens

Total Magnification = Ocular Lens X Objective Lens

Objective Lens

• Red Band = Low Power = 4

4(obj lens) X 10(ocular lens) = 40X

• Yellow Band = Medium Power = 10Yellow Band = Medium Power = 10

10(obj lens) X 10(ocular lens) = 100X10(obj lens) X 10(ocular lens) = 100X

• Blue Band = High Power = 100Blue Band = High Power = 100

40(obj lens) X 10(ocular lens) = 400X40(obj lens) X 10(ocular lens) = 400X

• White Band = Oil Immersion

Follow the Rules!

• ALWAYS begin on LOW POWER– Once you have the specimen in focus– Move to medium power– Refocus, then you can move to high power

• Once you move off low power, you can NEVER, NEVER, NEVER use the coarse adjustment

Orientation of Specimen

Specimen is flipped horizontally & vertically

Depth of Field

Preparing a Wet Mount

Cover Slip 45O

Slowly LowerCover Slip

Air Bubbles - Try Again!

Types of Cells

• There are 2 types of cells:

Prokaryotic Eukaryotic (pro- before) (eu- true)

-kary = nucleus

Prokaryotic Cells

Prokaryotic Cells

Prokaryotic cells -lack a membrane-bound nucleus.-genetic material is present in the nucleoid

ALL prokaryotic cells are bacteria, and ALL bacteria are prokaryotic

The only place you will find prokaryotic cells in is the KINGDOM BACTERIA AND ARCHEBACTERIA

Eukaryotic Cells

Epithelial - Cheek Cells

The Cell Cycle

• Interphase

• Mitosis– Prophase - prepare– Metaphase - middle– Anaphase - apart– Telophase - two

• Cytokinesis cyto- = cell -kinesis = to move

The Cell Cycle

Interphase

Mitosis - Prophase

Mitosis - Metaphase

Mitosis - Metaphase

Mitosis - Anaphase

Mitosis - Anaphase

Mitosis - Telophase

Mitosis - Telophase/Cytokinesis

Practice Identifying the Stages of Mitosis

• Mitosis

Select the link below (you must be in “slide show” mode)If you are unable to view a slide show, you can go toBookmarks on Ocean Cruiser. The link is found there.