LC/MS/MS Solutions for Quantitation

API 5000TM

LC/MS/MS SystemThe world’s most sensitive LC/MS/MS system

A new benchmark for quantitative small molecule analysis.

The API 5000™ LC/MS/MS system is today’s most sensitive triple quadrupole mass

spectrometer for complex bioanalytical samples. Designed to deliver the lowest

limits of detection for the most demanding DMPK and ADMET studies, the system

combines new QJet™ ion guide technology with the proven Turbo V™ ion source

and a powerful new generation of Analyst® software. The result is a robust, high-

throughput platform for every phase of pharmaceutical development, from drug

discovery through clinical trials—and a new level of quantitative performance in a

reliable, easy-to-use system.

The API 5000™ LC/MS/MS system is the latest atmospheric pressure ionization (API) mass spectrometer from Applied Biosystems/MDS SCIEX. Backed by an unmatched track record of experience and performance in pharmaceutical development applications, this is the most sensitive—and the most powerful—triple quadrupole system we have ever produced.

Advanced technology in a class by itself.

Innovative Turbo V™ ion source Efficiently ionizes compounds and virtually eliminates cross-contamination,

even with large sample loads. Embedded ceramic heater technology and

improved gas dynamics contribute to the system’s low detection limits,

and enable highest sensitivity quantitation over the wide range of flow

rates used in pharmaceutical analyses.

Quick-change ESI and APCI probes let you switch between ionization

modes in seconds.

DuoSpray™ ion sourceThe optional DuoSpray™ ion source contains both ESI and APCI inlets,

allowing computer-controlled optimization of ionization parameters for

each compound during an LC run, making it an ideal tool for fast method

development as well as increasing throughput and data quality.

PhotoSpray® ion source An additional option for atmospheric pressure photo-ionization, the

PhotoSpray source expands the range of compounds that can be analyzed,

often at higher sensitivities than ESI or APCI.

Proprietary Curtain Gas™ interfaceReduces the need for routine maintenance and ensures maximum uptime

and productivity by protecting the interface region and quadrupole analyzer

from contamination.

IQ2 IQ3 DETECTOR

CEM

EXIT LENSST ST2 ST3Q0 High Pressure CellQJet™ Ion Guide Q1 LINAC® Collision Cell Q3

Orifice IQ1

Curtain Gas™ Interface

CurtainPlate

Source

Advanced technology in a class by itself.

IQ2 IQ3 DETECTOR

CEM

EXIT LENSST ST2 ST3Q0 High Pressure CellQJet™ Ion Guide Q1 LINAC® Collision Cell Q3

Orifice IQ1

Curtain Gas™ Interface

CurtainPlate

Source

Revolutionary new QJet™ ion guide Replaces the skimmer in earlier triple quad designs, dramatically

improving sensitivity. QJet ion guide technology captures more

ions that enter the instrument, more efficiently separates ions from

neutrals, and focuses them into Q0.

Patented LINAC® collision cell Ensures maximum ion transfer—free of cross-talk—in MS/MS

mode, allowing simultaneous multi-compound analyses and enabling

you to monitor more compounds and MRM (multiple reaction

monitoring) transitions without any appreciable loss in signal. LINAC

collision cell technology enables fast scanning without compromising

performance in all modes of operation, including product ion,

precursor ion, and neutral loss scans.

High throughput for the networked lab

Applied Biosystems/MDS SCIEX combines the world’s

most sensitive mass spectrometer with the industry’s leading

software for robust, high performance quantitation. Analyst®

software delivers a new standard in compliance, throughput,

and ease-of-use.

The best quantitation tools for rapid results—reduce your integration method development and data review time

Rapid acquisition method development—automated compound optimization and tuning routines

Intelligent integration—new, parameter-free algorithms reduce time spent revising and reviewing results

The most comprehensive suite of features available for 21

CFR Part 11 compliance—complete, role-based security, auditing, and electronic records

Centralized security management—power and flexibility in one easy-to-use console provides the finest security model for regulated labs

Powerful auditing features—complete, easily managed auditing tools give you project-based auditing on your terms, for all levels of compliance

Direct network acquisition—eliminate manual file management and enhance your data security by acquiring data directly over your network

Support for your workflow—from LIMS to processing tools, Analyst software has the flexibility to be integrated quickly into your environment

Without IntelliQuan

With IntelliQuan

The IntelliQuan integration algorithm provides a new level of confidence in automatic integration. Produce high quality results without having to set integration parameters in advance. IntelliQuan minimizes manual intervention during integration and speeds up data review, reducing your data analysis time to improve your lab’s productivity.

Excellent linearity over a wide dynamic range is illustrated in this calibration curve of eight replicate injections at each concentration of buspirone. From the table, the statistics and accuracy demonstrate unparalleled consistency and reproducibility required for quantitation in regulated environments.

Over two days and 500 plasma sample injections, the API 5000™ system demonstrates excellent reproducibility and stability for the response of the compound and internal standard.

Ideal for small molecule analysis

The API 5000™ LC/MS/MS system gives you unequalled

accuracy and precision for quantitative analysis. It also

offers linearity over a wide dynamic range and exceptional

sensitivity for routine quantitation of low abundance

compounds in complex matrices—delivering greater signal-

to-noise than the industry-standard API 4000™ system.

This chromatogram acquired at unit resolution of both Q1 and Q3 demonstrates outstanding S/N for 10 fg of buspirone injected on column. This data is taken from one of the eight replicate injections of the lowest concentration in the calibration curve shown in the figure at right. At this level, the %CV is an impressive 3.5.

Expert results—even for non-experts

This is not only the most powerful triple quad available;

it is also the easiest to use. From automated methods

development to quick, simple routine maintenance, the

API 5000 system is designed to make it easy for you to

get the answers you need, even if you are just getting

into mass spectrometry.

Unattended, high-throughput operation

The API 5000 system is engineered for continuous duty

operation in high-throughput regulated laboratories. With

a full complement of advanced automation features, it fits

seamlessly into your workflow, and requires limited user

intervention.

= Alprazolam 2.5 pg/µL MRM transition 308 281 CV 3.1%

= internal standard corrected d5-Alprazolam 1 pg/µL MRM transition 314 286 CV 2.5%

Injection Number

Res

pons

e

Internal Standard A

rea Ratio

r=0.9999

Max 4849.0 cps

Time, min

Inte

nsity

, cps

Concentration, ng/ml

Are

a, c

ount

s

Bringing innovation to life science mass spectrometryMass spectrometry is a critical and core enabling technology for the life

sciences. From basic research to drug discovery and development, clinical

research, forensic, food, and environmental analysis, mass spectrometry-based

methods play a key role.

When you choose Applied Biosystems/MDS SCIEX as your partner, you’re

choosing to work with the leading developer of LC/MS/MS technologies. We are

committed to remaining at the forefront of life science research by providing you

with the tools you need to succeed.

Support You Can Depend On—Whenever and Wherever You Need It

Our LC/MS/MS systems are backed by one of the world’s most extensive

service and support organizations staffed by professionals who are trained and

dedicated to keep your system running at peak performance and productivity

24 hours a day, seven days a week.

Maximize Instrument Uptime with Applied Biosystems BioMonitor® Service

With our BioMonitor Service, we can remotely and proactively track critical

system parameters over the Internet and identify potential instrument problems

before they affect your lab’s efficiency. Using a combination of remote monitoring,

preemptive monitoring and remote diagnostics, our award-winning BioMonitor

Service is helping many of our customers achieve a significant increase in

instrument uptime.

What could your lab do with the world’s most sensitive LC/MS/MS system?

We’d like to help you consider the possibilities. For more information about the

API 5000™ system, call the Applied Biosystems sales office nearest you, or visit

http://www.appliedbiosystems.com/5000

For Research Use Only. Not for use in diagnostic procedures.

Applied Biosystems, AB (Design), and BioMonitor are registered trademarks and Applera is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries. Analyst, LINAC, PhotoSpray, and TurboIonSpray are registered trademarks and API 5000, API 4000, Curtain Gas, DuoSpray, QJet, and TURBO V are trademarks of Applied Biosystems/MDS SCIEX, a joint venture between Applera Corporation and MDS Inc. MDS and SCIEX are registered trademarks of MDS Inc. All other trademarks are the sole property of their respective owners.

© Copyright 2006 Applera Corporation and MDS Inc. All Rights Reserved.

Printed in the USA, 04/2006 Publication 114BR15-02

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