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Phytophthora cinnamomi susceptibility trials 2007–2008 __________________________________________________________________________________________ 10 Appendix 1. Plant metadata Full plant details are held by the Royal Tasmanian Botanical Gardens under the RTBG accession number. Propagation year is given by the first two digits of the accession number. Species RTBG accession Origin Block Tag Pot size Control G1 6” pot Control G2 3” square tube Control G3 6” pot Treatment G4 8” pot Treatment G5 6” pot Treatment G6 6” pot Treatment G7 6” pot Boronia gunnii 04.0041 Meadstone Falls Treatment G8 1” tube Control H1 6” pot Control H2 3” square tube Treatment H3 5” pot Treatment H4 6” pot Boronia hemichiton 04.0051 Mt Arthur Treatment H5 6” pot Control P1 6” pot Treatment P2 6” pot 04.0042 Horseshoe Marsh Treatment P3 6” pot Control P4 6” pot Treatment P5 6” pot Boronia hippopala 04.0043 Dukes Marsh Treatment P6 6” pot 01.0978 Mt Mayson, Coles Bay Site 2 Control F1 8” pot 02.0043 Mt Mayson, Coles Bay 6063 55GFP - 066319 Control F2 8” pot 02.0496 Mt Mayson, Coles Bay 6063, 55GFP - 066319 Treatment F3 6” pot 02.0625 Mt Mayson, Coles Bay Site 2 Cutting of 01.0978 Treatment F4 6” pot 02.0692 Mt Mayson, Coles Bay 6063, 55GFP - 046321 Treatment F5 6” pot Control F6 6” pot 03.0023 Mt Mayson, Coles Bay 6063, 55GFP - 066319 Treatment F7 6” pot Control F8 6” pot 03.0051 Mt Mayson, Coles Bay – no details Treatment F9 6” pot 05.0586 Mt Mayson, Coles Bay tissue culture, 6063, 55GFP - 066319 Treatment F10 5” pot 05.0587 Mt Mayson, Coles Bay, tissue culture 6063, 55GFP - 066319 Treatment F11 5” pot Philotheca freyciana 05.0590 Mt Mayson, Coles Bay tissue culture - no details. Treatment F12 5” pot Not specified Commercial nursery Control A1–A6 2” tube Aotus ericoides Not specified Commercial nursery Treatment A7– A17 2” tube Not specified Commercial nursery Control L1–L6 2” tube Leptospermum scoparium Not specified Commercial nursery Treatment L7–L17 2” tube

Appendix 1. Plant metadata - Department of Primary ...dpipwe.tas.gov.au/Documents/Section2_P.cinnamomi_Boronia.pdf · Appendix 1. Plant metadata Full plant details are held by the

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Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

10

Appendix 1. Plant metadata

Full plant details are held by the Royal Tasmanian Botanical Gardens under the RTBG accessionnumber. Propagation year is given by the first two digits of the accession number.

Species RTBGaccession

Origin Block Tag Potsize

Control G1 6” pot

Control G2 3”squaretube

Control G3 6” pot

Treatment G4 8” pot

Treatment G5 6” pot

Treatment G6 6” pot

Treatment G7 6” pot

Boronia gunnii 04.0041 Meadstone Falls

Treatment G8 1” tube

Control H1 6” pot

Control H2 3”squaretube

Treatment H3 5” pot

Treatment H4 6” pot

Boronia hemichiton 04.0051 Mt Arthur

Treatment H5 6” pot

Control P1 6” pot

Treatment P2 6” pot

04.0042 Horseshoe

Marsh

Treatment P3 6” pot

Control P4 6” pot

Treatment P5 6” pot

Boronia hippopala

04.0043 Dukes Marsh

Treatment P6 6” pot

01.0978 Mt Mayson, Coles Bay Site 2 Control F1 8” pot

02.0043 Mt Mayson, Coles Bay

6063 55GFP - 066319

Control F2 8” pot

02.0496 Mt Mayson, Coles Bay

6063, 55GFP - 066319

Treatment F3 6” pot

02.0625 Mt Mayson, Coles Bay Site 2Cutting of 01.0978

Treatment F4 6” pot

02.0692 Mt Mayson, Coles Bay

6063, 55GFP - 046321

Treatment F5 6” pot

Control F6 6” pot03.0023 Mt Mayson, Coles Bay

6063, 55GFP - 066319 Treatment F7 6” pot

Control F8 6” pot03.0051 Mt Mayson, Coles Bay – nodetails Treatment F9 6” pot

05.0586 Mt Mayson, Coles Bay tissueculture, 6063, 55GFP - 066319

Treatment F10 5” pot

05.0587 Mt Mayson, Coles Bay, tissueculture 6063, 55GFP - 066319

Treatment F11 5” pot

Philotheca freyciana

05.0590 Mt Mayson, Coles Bay

tissue culture - no details.

Treatment F12 5” pot

Not specified Commercial nursery Control A1–A6 2” tubeAotus ericoides

Not specified Commercial nursery Treatment A7–A17

2” tube

Not specified Commercial nursery Control L1–L6 2” tubeLeptospermumscoparium Not specified Commercial nursery Treatment L7–L17 2” tube

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

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Appendix 2. Potting mixes for trial plants

(1) Threatened plants

Native mix per cubic metre: Pine Bark 85%Coarse Sand 5%Coco Peat 5%Dolomite 5 kgSoil Wetting Granules 1kgFerrous Sulphate 1.5 kgIBDU (Nitrogen) 1 kgMacracote Grey 4 kg (NPK 18:2:10)

(2) Commercial plants

Unknown pine bark based potting mix.

Ph

yto

ph

tho

ra c

inna

mo

mi susceptibility trials 2007–2008

___________________________________________________________________________________________________________________________________________12

Ap

pe

nd

ix 3

. P

lan

t h

ealt

h d

ata

(1) Phytophthora cinnamomi

ino

cu

late

d p

lan

ts: lodged at glasshouse 11 May 2007 — first inoculation on 26 November 2007, second on 10 December 2007.

TA

G26/1

1/0

710/1

2/0

717/1

2/0

72/1

/08

3/1

/08

16/1

/08

18/1

/08

29/1

/08

12/2

/08

18/2

/08

28/2

/08

11/3

/08

26/3

/08

8/4

/08

29/4

/08

21/5

/08

G4

AA

AA

AA

AA

AA

AA

A

G5

AA

AA

AA

AA

AA

AA

A

G6

BB

BB

BA

BB

BB

BB

B

G7

AA

AA

AA

AA

AA

AB

B

G8

AA

AA

AA

AA

AB

BB

A

H3

AB

AA

FP

c +

ve

H4

AA

BB

BB

BC

CB

BB

B

H5

AA

BB

BA

DC

EF

Pc -

ve

rep

eat

Pc -

ve

P2

AA

BC

CC

CC

CC

CC

C

P3

AA

BB

AB

AB

BB

BB

B

P5

AB

BB

AB

AA

BB

BB

B

P6

BB

CC

CC

BB

BB

BB

B

F3

BB

CD

EE

DD

DD

DD

D

F4

AB

BC

DD

EF

FF

Pc -

ve

rep

eat

Pc -

ve

F5

BC

FE

FP

c -

ve

F7

AB

CD

EE

ED

DD

DD

E

F9

BB

BC

CC

CC

CC

CC

C

F10

AB

BB

EE

EE

EE

EE

E

F11

BB

BC

CC

CC

CC

CC

B

F12

AA

BB

BB

BC

CC

CC

C

Ph

yto

ph

tho

ra c

inna

mo

mi susceptibility trials 2007–2008

___________________________________________________________________________________________________________________________________________13

Phytophthora cinnamomi

ino

cu

late

d p

lan

ts (

co

nti

nu

ed

)

TA

G26/1

1/0

710/1

2/0

717/1

2/0

72/1

/08

1/3

/08

3/1

/08

18/1

/08

29/1

/08

12/2

/08

18/2

/08

28/2

/08

11/3

/08

26/3

/08

8/4

/08

29/4

/08

21/5

/08

A7

BB

DF

FP

c +

ve

A8

BC

DE

EF

Pc +

ve

A9

AB

FF

FP

c +

ve

A1

0B

BB

EF

Pc +

ve

A1

1A

AE

FF

Pc +

ve

A1

2A

AE

FF

Pc +

ve

A1

3A

AE

EF

Pc +

ve

A1

4A

BB

EF

Pc +

ve

A1

5A

BE

EF

Pc +

ve

A1

6A

AE

EF

Pc +

ve

A1

7A

BC

EF

Pc +

ve

L7

AA

AA

AA

BA

BB

BB

B

L8

DD

DB

BB

BC

CC

CC

C

L9

AA

BB

BB

BA

BA

AA

A

L10

AA

BB

BB

BB

BB

BB

A

L11

AA

AA

AA

AB

BB

BB

B

L12

AB

BA

AA

BB

BB

BB

B

L13

AB

BC

CB

BB

BB

BB

A

L14

AA

AA

AB

BB

BB

BB

B

L15

CC

BB

BB

BA

BB

BB

B

L16

AB

BF

FF

Dead

plated

Pc +

ve

L17

AA

AA

BB

BA

BB

BB

B

Ph

yto

ph

tho

ra c

inna

mo

mi susceptibility trials 2007–2008

___________________________________________________________________________________________________________________________________________14

Co

ntr

ol

pla

nts

(u

ntr

ea

ted

)

TA

G26/1

1/0

710/1

2/0

717/1

2/0

72/1

/08

16/1

/08

29/1

/08

12/2

/08

28/2

/08

11/3

/08

26/3

/08

8/4

/08

29/4

/08

21/5

/08

G1

AA

AA

AA

AB

BB

BB

A

G2

AA

AA

AA

AA

AD

EE

F

G3

AA

AA

AA

BA

AA

AA

A

H1

CC

CC

EF

FP

c -

ve

H2

AA

AA

AA

AA

AA

AA

A

P1

AA

CB

BB

CB

BA

AA

B

P4

AA

BB

AA

BA

BB

BB

B

F1

BB

CC

CC

CC

CC

CC

C

F2

BB

BB

BB

BC

CC

DC

C

F6

BB

BB

BB

BC

CC

CC

B

F8

AA

BC

CC

CC

CC

CC

C

A1

BB

BB

BB

BB

BB

BB

B

A2

AA

BB

BB

BA

BB

BB

B

A3

CC

CC

CC

CC

CC

CC

C

A4

BB

BB

CC

BA

BB

BB

B

A5

BB

BB

BB

BA

AB

BB

A

A6

CB

BB

BB

BA

BB

BC

C

L1

CC

CD

DD

DC

CC

BB

B

L2

CB

BB

BB

BB

CB

BB

B

L3

AA

AB

BA

AA

BB

BB

A

L4

CC

CC

CC

CC

CC

CC

B

L5

DC

DD

DD

CC

CC

DD

B

L6

BB

CC

BB

BB

BB

BB

B

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

15

Appendix 4. Treated plants at completion of trial

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

16

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

17

Appendix 5. Control plants at completion of trial

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

18

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

19

Appendix 6. Root isolation records at completion of trial

Results of the root isolations from surviving inoculated plants at completion of the trial.

+ve = positive isolation result for Phytophthora cinnamomi; -ve = negative isolation result.

Plant Tag Fine root < 1mm Roots >1mm and collar

F1 -ve -ve

F2 -ve -ve

F3 -ve -ve

F4 -ve -ve

F5 -ve -ve

F6 -ve -ve

F7 -ve -ve

F8 -ve -ve

F9 -ve -ve

F10 -ve -ve

F11 +ve -ve

P1 -ve -ve

P2 -ve -ve

P3 -ve -ve

P4 -ve -ve

P5 -ve -ve

P6 -ve -ve

H1 -ve -ve

H2 -ve -ve

H3 -ve -ve

H4 -ve -ve

G1 -ve -ve

G2 -ve -ve

G3 -ve -ve

G4 -ve -ve

G5 -ve -ve

G6 +ve +ve

L14 +ve +ve

Phytophthora cinnamomi susceptibility trials 2007–2008__________________________________________________________________________________________

20

Resource Management &Conservation Division

Department of Primary Industriesand Water

GPO Box 44, Hobart 7001

Ph: 1300 368 550

Visit:www.dpiw.tas.gov.au