Aquarium Biogeography and Succession of Microbial Communities in Aquatic Built Environments...
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Aquarium Biogeography and Succession of Microbial Communities in Aquatic Built Environments Nitrification Results in Coral Pond 1 The nitrite levels in
Aquarium Biogeography and Succession of Microbial Communities
in Aquatic Built Environments Nitrification Results in Coral Pond 1
The nitrite levels in the water rose until sediment from another
tank was added on Day 12.5. The inoculum possibly contained
microbes capable of converting nitrite to nitrate, resulting in a
sudden increase in nitrate levels The following chart was generated
using PHINCH alpha to show the microbial composition of the water
samples as nitrate levels increase in the coral pond This visual
provides a good example of the correlation between microbial
chemistry data and the microbial community As nitrate levels
increase, the genus Tenacibaculum seems to take over the samples
Tenacibaculum species have been found to be fish pathogens, but no
fish were present in the coral pond The tropical tank that was used
as the inoculum did contain fish, but there was not a significant
amount of Tenacibaculum present, according to the limited data
analyzed by PHINCH Nitrification Results in Coral Pond 1 The
nitrite levels in the water rose until sediment from another tank
was added on Day 12.5. The inoculum possibly contained microbes
capable of converting nitrite to nitrate, resulting in a sudden
increase in nitrate levels The following chart was generated using
PHINCH alpha to show the microbial composition of the water samples
as nitrate levels increase in the coral pond This visual provides a
good example of the correlation between microbial chemistry data
and the microbial community As nitrate levels increase, the genus
Tenacibaculum seems to take over the samples Tenacibaculum species
have been found to be fish pathogens, but no fish were present in
the coral pond The tropical tank that was used as the inoculum did
contain fish, but there was not a significant amount of
Tenacibaculum present, according to the limited data analyzed by
PHINCH Acknowledgements I would like to thank the Alfred P. SLOAN
Foundation, the DNA Technologies Core facility at the UCD Genome
Center, Holly Bik and Jonathan Eisen. Acknowledgements I would like
to thank the Alfred P. SLOAN Foundation, the DNA Technologies Core
facility at the UCD Genome Center, Holly Bik and Jonathan Eisen.
What is the Built Environment? The built environment environment
refers to our cars, schools, offices, water pipes, etc These are
the areas we spent most of our time, yet we do not know much about
the microbial composition of these environments Aquariums are
habitats that bridge the natural and built environments, and that
is why they were the focus of this work What is the Built
Environment? The built environment environment refers to our cars,
schools, offices, water pipes, etc These are the areas we spent
most of our time, yet we do not know much about the microbial
composition of these environments Aquariums are habitats that
bridge the natural and built environments, and that is why they
were the focus of this work Research Questions In this study, we
aim to better understand the biogeography and succession of the
microbial communities inhabiting a pair of newly established tanks
in the UC Davis Biological Sciences Teaching Laboratory We utilize
16S rRNA PCR surveysa culture-independent, DNA-based sequencing
methodto answer 2 questions: 1)How does the microbial composition
of newly established aquarium systems change over time? 2)How do
the environmental conditions (temperature, salinity, pH, oxygen and
nutrient concentrations) correlate with these changes? Research
Questions In this study, we aim to better understand the
biogeography and succession of the microbial communities inhabiting
a pair of newly established tanks in the UC Davis Biological
Sciences Teaching Laboratory We utilize 16S rRNA PCR surveysa
culture-independent, DNA-based sequencing methodto answer 2
questions: 1)How does the microbial composition of newly
established aquarium systems change over time? 2)How do the
environmental conditions (temperature, salinity, pH, oxygen and
nutrient concentrations) correlate with these changes? Sabreen
Aulakh, Lakshmi Bharadwaj, Akshay Sethi, Alex Alexiev, Andrew
Shaver, Matthew Haggerty, Jennifer Flanagan, Jenna Morgan Lang,
David Coil, Ph.D. Department of Evolution and Ecology University of
California, Davis, 95616 Methods We took sediment, water and wall
samples from two newly established coral ponds every day from
December 2012- March 2013 Water chemistry data including nitrite,
nitrate, phosphate, ammonia, pH, salinity, and temperature was
collected each day Day 12.5 = Sediment from previously established
tropical tank was added to coral pond as the inoculum 3 technical
replicate samples were taken from each sampling period The
following chart explains sampling methods: Samples were sequenced
and then analyzed using QIME and PHINCH alpha Methods We took
sediment, water and wall samples from two newly established coral
ponds every day from December 2012- March 2013 Water chemistry data
including nitrite, nitrate, phosphate, ammonia, pH, salinity, and
temperature was collected each day Day 12.5 = Sediment from
previously established tropical tank was added to coral pond as the
inoculum 3 technical replicate samples were taken from each
sampling period The following chart explains sampling methods:
Samples were sequenced and then analyzed using QIME and PHINCH
alpha SedimentWaterWall Profile of sediment in 1.5 ml tubes 1L
water sample vacuum filtered through 1 micron filter KimWipe swipe
of the sides of the tank Why 16S PCR? 16S rRNA highly conserved in
all bacteria Species-specific microbial gene sequences Community
profiles tells us the make-up of ecosystem Why 16S PCR? 16S rRNA
highly conserved in all bacteria Species-specific microbial gene
sequences Community profiles tells us the make-up of ecosystem Next
Steps The findings discussed here serve as a preview to the type of
results we aim to obtain after sequencing and analyzing all 500
samples This preliminary data will serve as the model for further
analysis We plan to characterize the microbial community in both
coral ponds and compare the microbial composition to that present
in the original tropical tank We will use PHINCH alpha and QIME to
generate visuals and better understand the succession of the
microbial community Next Steps The findings discussed here serve as
a preview to the type of results we aim to obtain after sequencing
and analyzing all 500 samples This preliminary data will serve as
the model for further analysis We plan to characterize the
microbial community in both coral ponds and compare the microbial
composition to that present in the original tropical tank We will
use PHINCH alpha and QIME to generate visuals and better understand
the succession of the microbial community Discussing the Areas for
Improvement PHINCH analyzed an OTU file that did not contain all
the data points we collected in the first run This made it
difficult to draw conclusions regarding the relationship between
some water chemistry data and microbial composition The completed
runs must be analyzed with all data points in order to provide
accurate and meaningful results Discussing the Areas for
Improvement PHINCH analyzed an OTU file that did not contain all
the data points we collected in the first run This made it
difficult to draw conclusions regarding the relationship between
some water chemistry data and microbial composition The completed
runs must be analyzed with all data points in order to provide
accurate and meaningful results Phosphate Results in Coral Pond 1
The phosphate levels in the water were constant until sediment from
another tank was added on Day 12.5. The phosphate levels then
immediately rose and followed a general upward trend The following
chart was generated using PHINCH alpha to show the microbial
composition of the water samples as the phosphate levels changed in
the pond No correlation could be observed between phosphate levels
and microbial composition, suggesting that more data points need to
be inserted and considered The genus Tenacibaculum and the order
Stramenopiles were present in high levels, but their roles, if any,
are unknown Phosphate Results in Coral Pond 1 The phosphate levels
in the water were constant until sediment from another tank was
added on Day 12.5. The phosphate levels then immediately rose and
followed a general upward trend The following chart was generated
using PHINCH alpha to show the microbial composition of the water
samples as the phosphate levels changed in the pond No correlation
could be observed between phosphate levels and microbial
composition, suggesting that more data points need to be inserted
and considered The genus Tenacibaculum and the order Stramenopiles
were present in high levels, but their roles, if any, are unknown
Nitrate levels (ppm) % Composition of Sample Nitrite Nitrite levels
(ppb) Sampling Date Nitrate Sampling Date Nitrate levels (ppm) %
Composition of Sample Phosphate levels (ppm) Tenacibaculum
Stramenopiles