Arachis genomics in South America

IBONE - ArgentinaGuillermo Seijo

Antonio Krapovickas

EMBRAPAPatricia GuimarãesSoraya Leal BertioliMarcio Moretzsohn

José Valls

UCBDavid Bertioli

Exclusively South American 80 described species(9 taxonomical sections) in diverse habitats

Most wild species are diploidcultivated peanut is tetraploid:

Where did it come from ?

Peanut’s origin

Sterile AB hybrid 2n = 20

(x 2)

Diploid species with “AA” genome

Diploid species with “BB” genome

n = 10 x n = 10

Arachis hypogaea

AABB

Spontaneous duplication

Cytogenetics and genomicsFISH and GISH

Guiding the use of new species for production of synthetics

Seijo et al. Am. J. Bot. 91: 1294–1303. 2004.Seijo et al. Am. J. Bot. in-press

Wild species:Many allelesDiploid

A. hypogaeaFew allelesTetraploid Wild alleles bring many new traits

and provide polymorphism for genetics

Maps for peanutUse wild polymorphismDissect the tetraploid problem2 diploid and one tetraploid population

A.ipaensisA.duranensis

ABA.hypogaea

A.stenosperma x A.magna

x

x (A.duranensis x A.ipaënsis )c

BAC (HindIII)c. 80,000 clonesc. 6.5x

BAC (HindIII)c. 80,000 clonesc. 6.5x

Leg1520.0

AS_24K#27.9

AS_20A#39.4S1A36B##41.6S1A36D##45.0AdRGA26B##53.5AsRGA14D#60.7

Leg044##70.4AS_27E#73.8P25M46_2As#76.2M14#82.9

P26M68_34###96.8

AS_26A116.8

TC7G10##130.3Leg930#135.4Leg14M_Gm139.2Ah-229144.1Leg156148.2Leg136##153.4RN10F4##155.3TC5A7##155.9gi-832##160.5PM49##161.2RN5H2##163.1Ah-745_2##169.6TC9E8##179.5

Seq11C8198.4

Leg050##211.3Ah-462As#212.1PM120##213.1RN9C2##214.6Ah-569##222.6Ah-408230.5

Leg062##249.0TC5C5Ad#249.6TC1H4##253.2RN12E1##260.0

P13M49_5##281.1P19M42_1###287.2

cp4cp4.2_2004

A4_diploidgi-6200.0gi-3850.5

gi-909##17.3

RN13G8###32.9Leg036##39.4AS_20F#46.1P25M46_4###60.5Leg076###67.1Ah-275###75.8Ah-614###76.3Leg088###77.4P18M45_4###82.0Ah-742###85.0Ah4-26###90.5PM45###90.6Ah20###91.0TC6E1###91.1PM36###91.2RM6E8###91.6PM65###93.4TC2B1###94.4TC3A12###100.4P20M51_8###101.1P18M44_7###110.7RM9A3###120.7RM2H10#138.0

AC3D7##154.4

Ah6-125##165.2

Leg224##174.0Leg083#179.0Leg175#185.2

AsRGA24A200.4

AdRGA9C210.6P25M41_5214.8

A5_diploidAD_15B0.0AD_17E3.5Leg0314.0AD_13D17.5

RN0_06Ad36.6TC1A837.4TC11A455.2TC1A255.9Leg09256.0RN0_61457.0Seq15D6_2As#57.1PM2458.4TC7C6Ad63.8gi-90667.1Leg045##72.4RGA_H180.0Leg346##82.5RN21H1#88.6Seq4H693.6P18M60_3#99.6Leg186#102.7Leg11M_Gm107.1TC3H7108.6AC2H11#109.9TC5A6As#110.0Leg081113.2Exportin123.0Leg203130.2Leg074#136.7Leg356149.7Leg12M_Gm163.5

cp6

A6_diploidRN17F120.0Ah38.1

TC3H2Ad19.7

Ap16633.3

P18M60_1##51.2P21M36_958.1

P19M42_6##68.3

Seq2H1177.6TC9B12Ad#81.2P18M45_15#88.5P22M34_5693.1P18M45_17100.8P13M49_9103.6P18M44_6109.8

Leg304##120.1

Ah-594As139.1TC9B8As142.0Ah-700As145.2Ah-745152.6

RN9A5171.5Seq3E5183.5Seq4C1190.2Seq2D1195.1Seq15D6#197.9Leg178203.2Seq12B2204.2Ah21207.6Ah2208.2Ah-193209.8Ap14209.9RN11E5210.9Leg047212.2TC2E11Ad213.9Leg187218.1TC7H9#230.1TC1D1240.7TC2D6250.8TC4G5Ad251.0

Ah-199269.0

Ah-330290.5gi-876298.5Leg298303.8

Leg034314.2

P25M41_1332.7

P20M37_1340.8

AD_13B357.6

gi-919376.9TC2E5383.4

Leg169##407.9

cp1

A1_diploidLeg2080.0RN2F123.0Leg1045.1

Leg128##22.4

AdRGA14A42.9Leg24255.2Leg33M_Gm61.4TC7H1165.8Leg160Leg15973.5Ah-07475.3TC4A278.3TC11A279.3Ah23_2As81.1AC3C282.3RN0_50684.6TC1G486.5Ah-659Ad90.4Leg20M_Gm100.7

RN0_729126.5

Ah11151.9

Leg177173.9TC4C8As183.4RN0_615187.8AC2C22As188.1Seq2G5188.4PM181188.9Leg202191.6Ah-573192.0RI1F6193.1TC4F12Ad193.8TC4D2193.9PM8196.2Leg213199.8PM230202.2PM32203.0TC4H2#216.0AD_17B#224.4H8ARGAAd#229.6AD_19C#237.2P22M3411248.9P25M46_11250.1P18M69_3251.9AdRGA27B257.7P18M60_2263.5RM17H9274.5Leg146291.7P21M3648292.2

PM22304.8

Leg069318.0RI2A6321.8LegTC3145324.6

P18M45_18334.8

P25M4110343.8

cp2

A2_diploidLeg188##0.0S1A37A##14.0P21M68_315.8S1A36_C20.8TC7E4###25.5RN3E10##29.1Leg066###29.6LegTC987##32.2P21M68_6##40.8TC4G2###41.0RM1A5###45.5Leg237###47.3gi-560###51.2LegRAB###60.8Ah30###65.5Ag99###75.2PM3###76.8TC0A1Ad##77.1TC4E10###77.5PM42##78.1Leg4Gm#80.4Leg181###80.7Leg223##81.0Leg168##81.5Seq13B7##86.1AsRGA15C##88.3Leg043##94.8PM238##107.1TC2C7##108.1Leg225##108.7TC1B2_2##113.0RN10F9##120.3TC1E6#121.6RN9C8##121.7Leg4amino126.5S48RGA2137.8AdRGA11F150.8PM18Ad173.9

Leg133193.1RI2D6197.1

TC2A2210.8RN8C9218.1RN23F10222.9

Seq4F10233.9TC3E2237.6

cp3_2005

A3_diploidLeg176##0.0

TC6G921.3

TC1D1As36.5Leg24M_Gm42.4RN22G7#49.1TC4G1052.1TC3G1_256.0TC9H8#57.7PM20460.3PM225As65.5PM243##66.4Leg14977.8

Leg05588.9

Leg29997.2

Leg228110.0

Leg196123.2

RN13D4134.6

A7_diploidgi-7160.0Leg0334.1TC9F45.6Ah-558TC4H2_2As6.3RM15C118.2TC1E511.8P25M46_718.5AD_21E23.8AD_25A28.3P21M36_729.3P16M48_329.4Leg00242.3P16M48_746.0gi-7295Ad51.8RN1A958.9PM18860.5TC6H361.0TC9F1061.6RN2H1167.7P21M68_474.9Leg17M_Gm91.2P18M4526102.4

RM5G08110.8

AdRGA14123.5

P25M46_8148.0AdRGA9A160.9AS_1A163.3AD_24C167.1RN22A12171.2Leg070172.2Leg305173.6Leg30M_Gm176.5Leg654177.0TC7A2178.8RM11H6185.4TC3B5#195.0Leg929209.5Leg924##212.4

P18M4556230.4P18M45_7231.4

P22M3415253.2

A8_diploid

Lec-1#0.0

gi-11709.8Ah-64921.2Leg32222.2PM170#24.5ML4D225.9Seq14G333.5

Leg1_Gm54.8Leg19956.4P21M36_260.5

AC2C273.6Ah-636As75.8

AS_11B89.8P25M46_9##93.3PM35##104.1AD_11G#109.1TC1D2##110.8RM13A12#115.7AD_24G#117.1AD_11D#117.5P18M69_1##118.1PM119#119.0Leg100#120.6TC7H2121.2TC1B2Ad121.5Ah-671##122.7gi-7295##138.5P16M48_1##154.6P13M49_1##161.5

AS_21C174.8

A9_diploid

TC2C120.0

RN0_68123.7gi-936#27.3gi-62328.2

RM15H8##40.1

TC4D9##58.4

AS_13C67.6

A10_diploid

186 SSRs (47% poly.) QTLs81 anchor markers leaf spot15 RGAs seed weight47 sequenced AFLPs seed number10 others> 50% genic51% distortion

Moretzsohn et al. TAG 111, 1060-1071, 2005 and unpublished

AA map

Arachis AA vs Medicago

Seq12B02#0.0

seq18G09#27.2seq4D04#38.5seq4A0642.7seq7B0944.0Ah344.3Ah1144.7TC7D0345.2IC40945.6Ah3947.0AC2C0848.1Ah-29652.8Seq13A0763.2Ap15276.7Seq19C03#99.5

B1

RN31D030.0TC1G0414.8AC3C0216.9TC4A02#17.4PM67519.4AC2D04#29.8TC7F04#46.8

TC7H1165.1

AC2B384.4

Ah28396.7

PM45136.2RN10B08146.9

B2

TC3E020.0

TC7E02#26.6Seq4F1037.8RN8C0947.3TC2A0250.1Ap17561.2SC3E10161.9seq2B1063.7RI2D0670.5SI01A579.3Ag14091.8TC1E0692.3RN10F0993.5seq16C07100.5TC11B11101.1seq2C11102.7gi-0090103.2PM3114.8Ah30#134.5

ML2A05160.8Ah35168.1IC377168.9Seq2D08#177.2RN3E10182.9TC7E04193.1

seq19D09219.1

B3

SD03B110.0

RN12E0122.1

SC05D0544.9TC5C0547.7

SI07G464.2TC11C0664.6seq3B1069.3PM3573.8TC4H07#87.0

Ag49#104.9

TC7G10#124.3

Seq13B09#139.8

Ah21#170.1Ah126#170.7Seq4B09#173.1

B4

Ah2820.0SI01D26.1

PM3232.4seq18B0139.4PM181#40.9Seq14F04#51.2RN31F06#55.8TC1E01#58.0Ag39#58.5seq14G03#74.1

RI2A06105.3seq2F05110.7RN22E12#117.7

B5

TC2E05-10.0

Ah347.7

RM14B11A68.6

Ah2107.3

Seq11H11-2A118.2Ah594120.6TC4H02124.3Ah21128.0Ah193130.9RN23A07135.4

4nA1

Seq4B11A0.0

gi93627.8gi62332.2

RM6F0339.0

PM2479.1RN34H1086.4TC9C0688.1

RN31A0598.2Seq4H6101.3

TC3H07A109.4

AC2A04133.6

4nA2

RN12E01A0.0

Ah40827.5

PM12037.7

RN5H0248.4RN23F1254.3

TC5A0763.6

Ah22979.9

RN31E11A114.0

4nA3

TC3E02A0.0

RI2D0634.4

RN10F0950.5TC2C0754.1

RN12H01A63.4PM4266.4

Sseq18A574.3

Ah3090.6

TC4G02A102.4

gi560112.5

4nA4

RN32F110.0TC7H02A1.2Sseq17E33.5TC5D069.2

RN35H0436.0

Lec1i65.2

Seq3E1074.6

Lec1A84.6

4nA5

TC7H020.0

RN22A1248.4

PM4588.7

RN31E11113.7

4nB1

TCE020.0

TC4G0253.4

TC4E1079.9

RM14B1194.5

4nB2

TC1B02i0.0

PM32B14.8

AC2F0824.7

Ag3954.6

RI2A0675.8

Seq2F584.2

4nB3

RN12H010.0

TC2G0554.9TC3B0561.4RM7E0463.3

TC3B0473.2

4nB4

Lec10.0

TC5A068.7

4nB5

PM360.0

PM5013.3

4nB6

RN17F120.0

Ah38.1

TC3H02Ad19.7

Ap16633.3

P18M60-151.2P21M36-958.1

P19M42-668.3

Seq2H1177.6TC9B12Ad81.2P18M45-1588.5P22M34-56*93.1P18M45-17100.8P13M49-9103.6P18M44-6109.8

Leg304120.1

Ah-594As139.1TC9B08As142.0Ah-700As145.2Ah-745_1152.6

RN9A05171.5Seq3E05183.5Seq4C01190.2Seq2D01195.1Seq15D06_1197.9Leg178203.2Seq12B02204.2Ah21207.6Ah2208.2Ah-193209.8Ap14209.9RN11E05210.9Leg047212.2TC2E11Ad213.9Leg187218.1TC7H09230.1TC1D01_1240.7TC2D06250.8TC4G05Ad251.0

Ah-199269.0

Ah-330290.5

gi-876298.5Leg298303.8

Leg034314.2

P25M41-1332.7

P20M37-1340.8

Ad13B357.6

gi-919376.9TC2E05383.4

Leg169407.9

A1Leg2080.0RN2F123.0Leg1045.1

Leg12822.4

Ad14A_RGA42.9Leg24255.2Leg33MGm61.4TC7H1165.8Leg160 Leg15973.5Ah-07475.3TC4A0278.3TC11A279.3Ah23_2As81.1AC3C0282.3RN0x50684.6TC1G0486.5Ah-659Ad90.4Leg20MGm100.7

RN0x729126.5

Ah11151.9

Leg177173.9TC4C08As183.4RN0x615187.8AC2C02_2As188.1Seq2G05188.4PM181188.9Leg202191.6Ah-573192.0RI1F06193.1TC4F12Ad193.8TC4D02193.9PM8196.2Leg213199.8PM230202.2PM32203.0TC4H02_1216.0Ad17B224.4AdH8A_RGA229.6Ad19C237.2P22M34-11248.9P25M46-11250.1P18M69-3251.9Ad27B_RGA257.7P18M60-2263.5RM17H09274.5

Leg146291.7P21M36-48*292.2

PM22304.8

LegTC3027 Leg069318.0RI2A06321.8LegTC3145324.6

P18M45-18334.8

P25M41-10343.8

A2TC3E20.0Seq4F103.7

RN23F1014.7RN8C919.6

TC2A226.8

RI2D640.5Leg13344.5

PM18Ad63.8

AdRGA11F86.8S48RGA299.9Leg4amino111.1TC11E4##115.9RN9C8## TC1E6#116.0RN10F9##117.3TC1B2_2##124.6Leg225##128.9TC2C7##129.6PM238##130.5Leg043##142.8AsRGA15C##149.4Seq13B7##151.5Leg168##156.1Leg223##156.6Leg181###156.9Leg4Gm#157.3PM42##159.6TC4E10###160.2TC0A1Ad##160.5PM3###160.8Ag99###162.4Ah30###172.1LegRAB###176.9gi-560###186.4Leg237###190.3RM1A5###192.1TC4G2###196.6P21M68_6##196.8LegTC987##205.4Leg066###208.1RN3E10##208.6TC7E4###212.1S1A36_C216.8P21M68_3221.8S1A37A##223.7Leg188##237.6

A3Leg1520.0

As24K27.9

As20A39.4S1A36B41.6S1A36D45.0Ad26B_RGA53.5

As14D_RGA60.7

Leg04470.4As27E73.8P18M44-56*76.2M1482.9

P26M68-34*96.8

As26A116.8

TC7G10130.3Leg930135.4Leg14MGm139.2Ah-229144.1Leg156148.2Leg136153.4RN10F04155.3TC5A07155.9gi-832160.5PM49161.2RN5H02163.1Ah-745_2169.6TC9E08179.5

Seq11C08198.4

Leg050211.3Ah-462As212.1PM120213.1RN9C02214.6Ah-569222.6Ah-408230.5

Leg062249.0TC5C05Ad249.6TC1H04253.2RN12E01260.0

P13M49-5281.1P19M42-1287.2

A4

XIP1050.0

XIP108A6.7

Ah15TC4G020.0

GM61836.7

Gi492651.9

GM74562.9

seq18A0567.5

seq19G0783.0

Ah16GM6240.0

seq19B125.4TC9H099.0

seq15C1020.1

Ah17seq11G030.0TC4F12_2A3.8

Ah18seq19D09_1B0.0

TC7E0435.4

Ah19GM6410.0

seq16G0847.3

Ah20

A3 diploid

B3 diploidA3 tetraploid

B3 tetraploid

A.hypogaea 16

Seq12B02#0.0

seq18G09#27.2seq4D04#38.5seq4A0642.7seq7B0944.0Ah344.3Ah1144.7TC7D0345.2IC40945.6Ah3947.0AC2C0848.1Ah-29652.8Seq13A0763.2Ap15276.7Seq19C03#99.5

B1

RN31D030.0TC1G0414.8AC3C0216.9TC4A02#17.4PM67519.4AC2D04#29.8TC7F04#46.8

TC7H1165.1

AC2B384.4

Ah28396.7

PM45136.2RN10B08146.9

B2

TC3E020.0

TC7E02#26.6Seq4F1037.8RN8C0947.3TC2A0250.1Ap17561.2SC3E10161.9seq2B1063.7RI2D0670.5SI01A579.3Ag14091.8TC1E0692.3RN10F0993.5seq16C07100.5TC11B11101.1seq2C11102.7gi-0090103.2PM3114.8Ah30#134.5

ML2A05160.8Ah35168.1IC377168.9Seq2D08#177.2RN3E10182.9TC7E04193.1

seq19D09219.1

B3

SD03B110.0

RN12E0122.1

SC05D0544.9TC5C0547.7

SI07G464.2TC11C0664.6seq3B1069.3PM3573.8TC4H07#87.0

Ag49#104.9

TC7G10#124.3

Seq13B09#139.8

Ah21#170.1Ah126#170.7Seq4B09#173.1

B4

Ah2820.0SI01D26.1

PM3232.4seq18B0139.4PM181#40.9Seq14F04#51.2RN31F06#55.8TC1E01#58.0Ag39#58.5seq14G03#74.1

RI2A06105.3seq2F05110.7RN22E12#117.7

B5

TC2E05-10.0

Ah347.7

RM14B11A68.6

Ah2107.3

Seq11H11-2A118.2Ah594120.6TC4H02124.3Ah21128.0Ah193130.9RN23A07135.4

4nA1

Seq4B11A0.0

gi93627.8gi62332.2

RM6F0339.0

PM2479.1RN34H1086.4TC9C0688.1

RN31A0598.2Seq4H6101.3

TC3H07A109.4

AC2A04133.6

4nA2

RN12E01A0.0

Ah40827.5

PM12037.7

RN5H0248.4RN23F1254.3

TC5A0763.6

Ah22979.9

RN31E11A114.0

4nA3

TC3E02A0.0

RI2D0634.4

RN10F0950.5TC2C0754.1

RN12H01A63.4PM4266.4

Sseq18A574.3

Ah3090.6

TC4G02A102.4

gi560112.5

4nA4

RN32F110.0TC7H02A1.2Sseq17E33.5TC5D069.2

RN35H0436.0

Lec1i65.2

Seq3E1074.6

Lec1A84.6

4nA5

TC7H020.0

RN22A1248.4

PM4588.7

RN31E11113.7

4nB1

TCE020.0

TC4G0253.4

TC4E1079.9

RM14B1194.5

4nB2

TC1B02i0.0

PM32B14.8

AC2F0824.7

Ag3954.6

RI2A0675.8

Seq2F584.2

4nB3

RN12H010.0

TC2G0554.9TC3B0561.4RM7E0463.3

TC3B0473.2

4nB4

Lec10.0

TC5A068.7

4nB5

PM360.0

PM5013.3

4nB6

RN17F120.0

Ah38.1

TC3H02Ad19.7

Ap16633.3

P18M60-151.2P21M36-958.1

P19M42-668.3

Seq2H1177.6TC9B12Ad81.2P18M45-1588.5P22M34-56*93.1P18M45-17100.8P13M49-9103.6P18M44-6109.8

Leg304120.1

Ah-594As139.1TC9B08As142.0Ah-700As145.2Ah-745_1152.6

RN9A05171.5Seq3E05183.5Seq4C01190.2Seq2D01195.1Seq15D06_1197.9Leg178203.2Seq12B02204.2Ah21207.6Ah2208.2Ah-193209.8Ap14209.9RN11E05210.9Leg047212.2TC2E11Ad213.9Leg187218.1TC7H09230.1TC1D01_1240.7TC2D06250.8TC4G05Ad251.0

Ah-199269.0

Ah-330290.5

gi-876298.5Leg298303.8

Leg034314.2

P25M41-1332.7

P20M37-1340.8

Ad13B357.6

gi-919376.9TC2E05383.4

Leg169407.9

A1Leg2080.0RN2F123.0Leg1045.1

Leg12822.4

Ad14A_RGA42.9Leg24255.2Leg33MGm61.4TC7H1165.8Leg160 Leg15973.5Ah-07475.3TC4A0278.3TC11A279.3Ah23_2As81.1AC3C0282.3RN0x50684.6TC1G0486.5Ah-659Ad90.4Leg20MGm100.7

RN0x729126.5

Ah11151.9

Leg177173.9TC4C08As183.4RN0x615187.8AC2C02_2As188.1Seq2G05188.4PM181188.9Leg202191.6Ah-573192.0RI1F06193.1TC4F12Ad193.8TC4D02193.9PM8196.2Leg213199.8PM230202.2PM32203.0TC4H02_1216.0Ad17B224.4AdH8A_RGA229.6Ad19C237.2P22M34-11248.9P25M46-11250.1P18M69-3251.9Ad27B_RGA257.7P18M60-2263.5RM17H09274.5

Leg146291.7P21M36-48*292.2

PM22304.8

LegTC3027 Leg069318.0RI2A06321.8LegTC3145324.6

P18M45-18334.8

P25M41-10343.8

A2TC3E20.0Seq4F103.7

RN23F1014.7RN8C919.6

TC2A226.8

RI2D640.5Leg13344.5

PM18Ad63.8

AdRGA11F86.8S48RGA299.9Leg4amino111.1TC11E4##115.9RN9C8## TC1E6#116.0RN10F9##117.3TC1B2_2##124.6Leg225##128.9TC2C7##129.6PM238##130.5Leg043##142.8AsRGA15C##149.4Seq13B7##151.5Leg168##156.1Leg223##156.6Leg181###156.9Leg4Gm#157.3PM42##159.6TC4E10###160.2TC0A1Ad##160.5PM3###160.8Ag99###162.4Ah30###172.1LegRAB###176.9gi-560###186.4Leg237###190.3RM1A5###192.1TC4G2###196.6P21M68_6##196.8LegTC987##205.4Leg066###208.1RN3E10##208.6TC7E4###212.1S1A36_C216.8P21M68_3221.8S1A37A##223.7Leg188##237.6

A3Leg1520.0

As24K27.9

As20A39.4S1A36B41.6S1A36D45.0Ad26B_RGA53.5

As14D_RGA60.7

Leg04470.4As27E73.8P18M44-56*76.2M1482.9

P26M68-34*96.8

As26A116.8

TC7G10130.3Leg930135.4Leg14MGm139.2Ah-229144.1Leg156148.2Leg136153.4RN10F04155.3TC5A07155.9gi-832160.5PM49161.2RN5H02163.1Ah-745_2169.6TC9E08179.5

Seq11C08198.4

Leg050211.3Ah-462As212.1PM120213.1RN9C02214.6Ah-569222.6Ah-408230.5

Leg062249.0TC5C05Ad249.6TC1H04253.2RN12E01260.0

P13M49-5281.1P19M42-1287.2

A4

XIP1050.0

XIP108A6.7

Ah15TC4G020.0

GM61836.7

Gi492651.9

GM74562.9

seq18A0567.5

seq19G0783.0

Ah16GM6240.0

seq19B125.4TC9H099.0

seq15C1020.1

Ah17seq11G030.0TC4F12_2A3.8

Ah18seq19D09_1B0.0

TC7E0435.4

Ah19GM6410.0

seq16G0847.3

Ah20

A3 diploid

B3 diploidA3 tetraploid

B3 tetraploid

A.hypogaea 16

Mt Lj

Anchors SSRs

Models Reference diploids Cultivated

University of Aarhus, Denmark

Jens Stougaard Leif Schauser

Birgit HougaardJakob Fredslund

Lene MadsenNiels Sandal

ICRISAT – IndiaCERAAS/ISRA – Senegal

UC Davis – USAUG – USAJIC - UK

CIRAD France

Angelique d'Hont Olivier Garsmeur

Jean-Francois Rami

Financial Support

Outside South America - Partners

Synthetic amphidiploi

ds

Genetic and Physical

mapsSynteny

Marker Assisted Selection in

breeding programs

Improved peanut varieties

SSRMarkers

Genome Anchor Markers

Candidate gene

Markers. QTLs

Cytogenetic analysis

Evaluation of

germplasm.

Genotyping

system

BAC libraries

Summary