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Aspen Chromatography Aspen Chromatography Hydrophobic Interaction Chromatography (HIC)Hydrophobic Interaction Chromatography (HIC)Chromatography (HIC)Chromatography (HIC)
Bioprocess LaboratoryDepartment of Chemical Engineering
Ch N ti l U i itChungnam National University
Introduction (HIC experimental data)(HIC experimental data)
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Simulation conditions
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Aspen chromatography i l ti simulation The step 1 (Loading step)The step 1 (Loading step)
Time : 10 min
The step 2 (Gradient step)Time : 15 min
The step 3 (Washing step)The step 3 (Washing step)Time : 30 min
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Buffer SimulationBuffer Simulation
Loading stepLoading step
Gradient step
washing step
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NP값변화NP값변화
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8IP3 : 8
NP : 30MTC : 100000
IP3 : 8NP : 300MTC : 100000
IP3 : 8NP : 30000MTC : 100000
IP3 : 8NP : 3000000MTC : 100000
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MTC값변화MTC값변화
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30
IP3 : 8NP : 30MTC : 1000
IP3 : 8NP : 30MTC : 100000
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값변화IP2값변화
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 1IP3 : 8
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 300IP3 : 8
NP : 30MTC : 100000
NP : 30MTC : 100000
NP : 30MTC : 100000
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IP3값변화IP3값변화
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 1
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8
IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 20
NP : 30MTC : 100000
NP : 30MTC : 100000
NP : 30MTC : 100000
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Q값변화Q값변화
Q = 0.15Q = 0.015 Q = 1.5
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Q = 15 Q = 1500
HIC Simulation Interim ResultHIC Simulation Interim Result
IP1(L T T h) : 10 15 20IP1(Lac-Trn-Tsh) : 10-15-20IP2 : 30IP3 : 8NP 30NP : 30MTC : 100000Q : 0.15
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분자량의 변화분자량의 변화
MW(molecular weight)
A, B, C = 10,000
MW(molecular weight)
A = 40,000B = 71,400C = 36,100
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HIC Simulation and Experiment
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