—The Next Generation FISH Technologykromat.hu/UserFiles/files/patologia/SureFISH_Catalog_US-12688.pdfA 50 kb deletion within the IKZF1 gene is clinically important in leukemia. A

~20,000 clones harboring known fragments of human DNA

Clone corresponding to region of interest is isolated.

Production of cloned DNA is scaled up in culture.

Cloned DNA is purified and labeled with fluorescent dye to generate probe.

Fluorescence in situ hybridization (FISH) is a laboratory procedure in which fluorescently labeled DNA fragments (the FISH probe) hybridize to complementary DNA sequences in the cell’s nucleus. The resulting fluorescent signals are visualized using a microscope and indicate the presence and localization of one or more targeted DNA sequences. Agilent offers three types of probes: break apart, dual fusion, and copy number.

Oligonucleotide–based FISH ProbesAgilent’s unique SureFISH DNA FISH probes are designed in silico and chemically synthesized using the company’s high-fidelity, oligonucleotide library synthesis (OLS) technology. This eliminates the limitations of FISH probes manufactured with bacterial artificial chromosome (BAC) technology. (See Panel A.) Agilent is the only comprehensive DNA FISH probe supplier that does not use BAC technology in probe manufacturing.

BAC-based FISH probes are derived from a library of bacterial clones, each harboring a DNA fragment corresponding to a specific ~150 kb region of the human genome. BAC FISH technology is limited first by clone availability. The human genome is comprised of 3 billion base pairs, yet a typical BAC library consists of only about 20,000 clones. This limits the choice of probes and may result in low specificity of the probe to the region of interest. Secondly, BAC FISH probes are derived from fragmented, human genomic DNA, so they include many naturally occurring repetitive sequences. A staggering fifty percent of the human genome consists of repetitive DNA, including mini- and microsatellites, and transposable elements.* Cot-1 DNA is included as a “blocker” to reduce the non-specific binding. However, BAC-based probes still often produce high hybridization background signal, characterized by hazy fluorescence across cell nuclei, and in some cases other non- specific signals. Blocker can also inhibit overall hybridization and lead to signal suppression.

*Smit, AFA, Hubley, R & Green, P. RepeatMasker Open-4.0.2013-2015. Figure 1. BAC probe production process.

Panel A. BAC FISH Probe Technology Limitations

SureFISH—The Next Generation FISH Technology

1 www.agilent.com/genomics/surefish

SureFish probes are Analyte Specific Reagents. Analytical and performance characteristics are not established.

A

B Agilent TP53 Probe Competitor TP53 Probe

High Probe Specificity—Precisely Target a Region of InterestDesigning probes on the computer “in silico” means complete design flexibility. An oligonucleotide (oligo) probe can be designed to precisely target any region of interest with base-pair level resolution (Figure 2). Precision probe design means higher probe specificity.

High Signal-to-Noise Ratio—Free of Repetitive DNASureFISH probes, because they are comprised of chemically synthesized oligos, do not suffer from high hybridization background and non-specific signal. To ensure that every SureFISH probe is truly repeat-free and will produce clean signal, each oligo is sequence compared to the entire human genome to verify target specificity. All non-specific sequences are removed. Thus, SureFISH probes eliminate the need for blocking and the signal suppression produced by blocking agents (Figure 3).

High Lot-to-lot Consistency—Free of Cell CultureAgilent’s synthetic SureFISH probe production process ensures high lot-to-lot consistency. It completely eliminates the biological BAC probe production process, which may cause lot-to-lot variation.

Figure 3. Repeat-free probe design. Panel A. The SureFISH TP53 probe avoids repetitive sequences, whereas the competitor BAC probe incorporates repetitive sequences. Panel B. Agilent TP53 probe produces bright clean signal, whereas the competitor’s BAC probe yields high background and non-specific hybridization.

BAC Probe Partial deletion

Complete deletionSureFISH Probe

Deletion not detectedSignal remains from overhang

Background noiseDeletion detectedBackground clear

BAC ProbeSureFISH Probe

2 3 4 5 6 7 8

50 kb

IKZF1exonsA

B

Ex 4-7 del

A

B

2www.agilent.com/genomics/surefish


Figure 2. SureFISH copy number probe enables precise targeting of a region of interest. A 50 kb deletion within the IKZF1 gene is clinically important in leukemia. A BAC probe targeting this region is not effective at detecting the small deletion because it incorporates sequences outside the targeted region. In contrast, SureFISH probes are designed to specifically target the deletion, producing clean signal. Copy number probes measure the copy number relative to a normal diploid state. Deletion is indicated by a single vs. dual signal in the nucleus.

Target Probe Type Part No. Description Coordinates μL

ALK Break apart G111400 SureFISH ALK BA chr2:29146786-29446528 20chr2:29446949-30045655

ROS1 Break apart G111401 SureFISH ROS1 BA chr6:116510643-117609677 20chr6:117747013-118899513

ROS1 GOPC Break apart G100952G SureFISH 6q22.1 ROS1 3' BA 1132kb GR chr6:116510648-117642558 5G100953R SureFISH 6q22.1 ROS1 5' BA 245kb RD chr6:117642558-117888013 5

RET Break apart G111402 SureFISH RET BA chr10:43465309-43609540 20chr10:43609847-44211442

MET (C-MET) Copy number G110900R SureFISH 7q31.2 MET 400kb RD chr7:116192143-116591613 5EGFR (EGFR1, Her1)

Copy number G101155R SureFISH 7p11.2 EGFR 188kb RD chr7:55086726-55275026 5

ALK/EML4 Dual fusion G110919R SureFISH 2p23.2 ALK 479kb P5RD chr2:28967568-29446398 5inv(2)(p21p23) G110920G SureFISH 2p21 EML4 496kb P5GR chr2:42054627-42550792 5

Her2 (ERBB2) Copy number G101022R SureFISH 17q12 HER2 191kb RD chr17:37787513-37978428 5Chr17 CEP Copy number G110910G SureFISH Chr17 CEP 436kb P5GR chr17:20892396-21327941 5TP53 17p Copy number G100950R SureFISH 17p13.1 TP53 120kb RD chr17:7529541-7650026 5SMSCR/RAI1 17p

Copy number G101152R SureFISH 17p11.2 RAI1 263kb RD chr17:17534296-17797781 5

RARA 17q Copy number G101093G SureFISH 17q21.1 RARA 5' BA 600kb GR chr17:37891978-38491658 5

ERG Break apart G101000R SureFISH 21q22.13 ERG 3' BA 228kb RD chr21:39605914-39833719 5G101001G SureFISH 21q22.2 ERG 5' BA 505kb GR chr21:39834224-40339324 5

TMPRSS2/ Dual fusion G110913G SureFISH 21q22.13 ERG DF 777kb P5GR chr21:39460929-40237724 5ERG G110914R SureFISH 21q22.3 TMPRSS2 DF 873kb P5RD chr21:42414894-43287887 5

pCatalog IDs G, R, and B designation in the catalog number represents Green, Orange-red, and Aqua color availability G ex: 495 nm; em: 517 nm, R ex: 547 nm; em: 565 nm, B ex: 429 nm; em: 470 nm




TMPRSS2/ Tri color G110915R SureFISH 21q22.13 ERG 3p BA 426kb P5RD chr21:39391099-39817049 5ERG

G110916G SureFISH 21q22.2 ERG 5p BA 552kb P5GR chr21:39817544-40369799 5G101082B SureFISH 21q22.3 TMPRSS2 5p TC 529kb BL chr21:42885784-43414707 5

pTEN Copy number G100936R SureFISH 10q23.31 PTEN 160kb RD chr10:89598246-89758016 5

MDM2 Copy number G101085R SureFISH 12q15 MDM2 180kb RD chr12:69137889-69317809 5FOXO1 (FKHR) Break apart G110904G SureFISH FOXO1 3' BA 600kb GR chr13:40529680-41129925 5

G110905R SureFISH FOXO1 5' BA 601kb RD chr13:41241360-41842395 5SS18 (SYT) Break apart G101123G SureFISH 18q11.2 SS18 3’ BA 616kb chr18:22989888-23606098 5

G101124R SureFISH 18q11.2 SS18 5’ BA 406kb chr18:23616888-24022533 5EWSR1 Break apart G101076R SureFISH EWSR1 3' BA 263kb RD chr22:29695821-29958416 5

G101077G SureFISH EWSR1 5' BA 564kb GR chr22:29100656-29664246 5DDIT3 (CHOP) Break apart G110922G SureFISH 12q13.3 DDIT3 3' BA 537kb P5GR chr12:57374319-57911594 5

G110923R SureFISH 12q14.1 DDIT3 5' BA 498kb P5RD chr12:57913109-58410999 5FUS Break apart G110929G SureFISH 16p11.2 FUS 5' BA 457kb GR chr16:30731376-31188501 5

G110930R SureFISH 16p11.2 FUS 3' BA 365kb RD chr16:31204526-31569981 5PAX3 Break apart G100947G SureFISH PAX3 3' BA 575Kb chr2:222490142-223064602 5

G100948R SureFISH PAX3 5' BA 285Kb chr2:223163717-223449157 5NCOA2/HEY1 Dual fusion G101101R SureFISH 8q13.3 NCOA2 DF 700kb RD chr8:70550024-71249639 5

G101102G SureFISH 8q21.13 HEY1 DF 774kb GR chr8:80507699-81281769 5





1p36 Copy number G101092R SureFISH 1p36.32 TPRG1L 332kb RD chr1:3372440-3704390 51q25 Copy number G101091G SureFISH 1q25.2 ABL2 600kb GR chr1:178780940-179380505 519p13 Copy number G101097G SureFISH 19p13.2 ZNF443 513kb GR chr19:12289833-12802573 519q13 Copy number G101098R SureFISH 19q13.33 GLTSCR2 298kb RD chr19:48102092-48400257 5TFE3 (XP11) Break apart G110906R SureFISH TFE3 3' RD chrX:48910016-49146851 5

G110907G SureFISH TFE3 5' GR chrX:48319071-48859081 5MYB Copy number G101078R SureFISH 6q23 MYB 251kb RD chr6:135401418-135652368 5CCND1 Copy number G101219B SureFISH CCND1 DF 577kb chr11:69140401-69717561 5nMYC (MYCN) (2p24)

Copy number G110921R SureFISH 2p24 MYCN 277kb P5RD chr2:15954938-16232273 5

FGFR2 Copy number G110918R SureFISH 10q26 FGFR2 360kb P5RD chr10:123117715-123477755 5

FGFR1 Copy number G101100R SureFISH 8p11 FGFR1 325kb RD chr8:38116069-38440924 5BRAF Break apart G101202R SureFISH 7q34 BRAF 3' BA 414kb chr7:140063482-140477872 5

G101203G SureFISH 7q34 BRAF 5' BA 510kb chr7:140500197-141009762 5

MALT1 Break apart G101038R SureFISH 18q21.32 MALT1 5' BA 300kb RD chr18:56037816-56337411 5G101039G SureFISH 18q21.32 MALT1 3' BA 603kb GR chr18:56421006-57023901 5

Dual fusion G101223R SureFISH 11q22.2 BIRC3 DF 674kb chr11:101865349-102539504 5G101224G SureFISH 18q21.32 MALT1 DF 672kb chr18:56040081-56712301 5

BCL2 Break apart G110924R SureFISH 18q21.33 BCL2 3’ BA 389kb P5 RD chr18:60400676-60789826 5

G110925G SureFISH 18q21.33 BCL2 5’ BA 598kb P5 GR chr18:60796936-61395251 5Dual fusion G110903G SureFISH 14q32.33 IGH DF 1519kb GR chr14:105767975-107286755 5

G101041R SureFISH 8q24.21 MYC DF 815kb RD chr8:128396131-129210696 5



BIRC3(API2)/MALT1 t(11;18)

IGH/MYC (CMYC) t(8;14)(q24;q32)



BCL6 Break apart G110926R SureFISH 3q27.3 BCL6 3’ BA 37kb P5 chr3:187067109-187438594 5G110931G SureFISH 3q28 BCL6 5’ BA 481kb P5 chr3:187735129-188216099 5

MYC (CMYC) Break apart G101228G SureFISH 8q24.21 MYC 5' BA 632kb GR chr8:128083886-128715471 5(Tri color) G101229R SureFISH 8q24.21 MYC 3' BA 432kb RD chr8:129132251-129555496 5

G101230B SureFISH 8q24.21 MYC 3' TC 398kb BL chr8:130342021-130739576 5CCND1 (BCL1) Break apart G101213G SureFISH 11q13.3 CCND1 3' BA 352kb GR chr11:69365371-69717561 5

G101214R SureFISH 11q13.3 CCND1 5' BA 311kb RD chr11:68407510-68718885 5

Dual fusion G110903G SureFISH 14q32.33 IGH DF 1519kb GR chr14:105767975-107286755 5

G101040R SureFISH 18q21.33 BCL2 DF 994kb RD chr18:60309881-61304141 5

IGH Break apart G101042G SureFISH 14q32.33 IGH 5' BA 641kb GR chr14:106651040-107288785 5G101043R SureFISH 14q32.33 IGH 3' BA 301kb RD chr14:105770335-106069355 5

Dual fusion G110903G SureFISH 14q32.33 IGH DF 1519kb GR chr14:105767975-107286755 5G100960R SureFISH 11q13.3 CCND1 997kb RD chr11:68900135-69897500 5

Dual fusion G110903G SureFISH 14q32.33 IGH DF 1519kb GR chr14:105767975-107286755 5G101117R SureFISH 16q23.2 MAF DF 604kb RD chr16:79281866-79885566 5



IGH/BCL2 t(14;18)(q32;q21)

IGH/MAF t(14;16)(q32.3;q23)

IGH/CCND1 t(11;14)(q13;q32)



Dual fusion G101030G SureFISH 22q11.21 BCR DF 1388kb GR chr22:23172171-24560506 5G101031R SureFISH 9q34.12 ABL DF 640kb RD chr9:133223275-133863495 5

MLL (KMT2A) Break apart G101050R SureFISH MLL 3' BA 199kb RD chr11:118350159-118548829 5G101051G SureFISH MLL 5' BA 497kb GR chr11:117837554-118334174 5

Dual fusion G101007R SureFISH 12p13.2 ETV6 505kb RD chr12:11678026-12182681 5

G101081G SureFISH 21q22.12 RUNX1 DF 672kb GR chr21:35959744-36632204 5

ETV6 BA Break apart G101080R SureFISH 12p13.2 ETV6 3' BA 384kb RD chr12:12036786-12421026 5G101079G SureFISH 12p13.2 ETV6 5' BA 460kb GR chr12:11575021-12035331 5

Dual fusion G101106R SureFISH 1q23.3 PBX1 DF 603kb RD chr1:164381330-164984670 5G101107G SureFISH 19p13.3 TCF3 DF 729kb GR chr19:1264345-1993710 5

CEP4 Copy number G101066B SureFISH Chr4 CEP 613kb BL chr4:48100271-48712926 5CEP10 Copy number G101084R SureFISH Chr10 CEP 676kb RD chr10:36335860-37011870 5CEP17 Copy number G110910G SureFISH Chr17 CEP P5GR chr17:20892396-21327941 5CDKN2A(p16 Copy number G100990R SureFISH 9p21.3 CDKN2A 57kb RD chr9:21954295-22011360 5del)(9p21) G101189R SureFISH 9p21.3 CDKN2A 188kb RD chr9:21901070-22089435 5IKZF1 Copy number G100989R SureFISH 7p12.2 IKZF1 62kb RD chr7:50410911-50472741 5

Dual fusion G101605 SureFISH PML RARA DF chr15:74150193-74600468 20chr17:37940463-39000653

RARA Break apart G101093G SureFISH 17q21.1 RARA 5' BA 600kb GR chr17:37891978-38491658 5G101094R SureFISH 17q21.2 RARA 3' BA 419kb RD chr17:38493428-38912408 5


PML/RARA t(15;17)(q22;q21)

ETV6/RUNX1 (TEL/AML1) t(12;21)(p13;q22)

TCF3/PBX1 (E2A/PBX1) t(1;19)(q23;p13)

BCR/ABL t(9;22)(q34.12;q11.23




5q31 del EGR1 Copy number G101036R SureFISH 5q31.2 EGR1 184kb RD chr5:137713653-137897633 55p D5S630 Copy number G101037G SureFISH 5p15.31 D5S630 370kb GR chr5:9400880-9768970 5

Dual fusion G101108R SureFISH 8q21.3 RUNX1T1 DF 734kb RD chr8:92670351-93404181 5G101081G SureFISH 21q22.12 RUNX1 DF 672kb GR chr21:35959744-36632204 5

CBFB (inv(16) Break apart G101028R SureFISH 16q22.1 CBFB 3' BA 240kb RD chr16:66874576-67114946 5(p13.3q22)) G101029G SureFISH 16q22.1 CBFB 5' BA 284kb GR chr16:67117501-67401671 5EVI1 (MECOM) Tri color G101204G SureFISH 3q26.2 MECOM 3' BA 451kb GR chr3:168349259-168799824 5

G101205R SureFISH 3q26.2 MECOM 5' BA 408kb RD chr3:168877769-169285769 5G101206B SureFISH 3q26.2 MECOM 5' TC 437kb BL chr3:169290714-169727389 5

NUP214 Break apart G101156G SureFISH 9q34.12 NUP214 5’ BA 548kb GR chr9:133450810-133998645 5G101157R SureFISH 9q34.13 NUP214 3’ BA 447kb RD chr9:134108745-134555830 5

17p13.1 TP53 (p53)

Copy number G100950R SureFISH 17p13.1 TP53 120kb RD chr17:7529542-7650026 5

11p22.3 (ATM) del

Copy number G101201G SureFISH 11q22.3 ATM 732kb GR chr11:107794684-108527139 5

13q14.3 del Copy number G101048R SureFISH 13q14.2 D13S319 154kb RD chr13:50584525-50738955 5CEP12 (D12Z3) Copy number G101090G SureFISH Chr12 CEP 704kb GR chr12:33231446-33935581 513q34 LAMP1 Copy number G101200B SureFISH 13q34 LAMP1 598kb BL chr13:113699929-114297659 513q34 LAMP1 Copy number G101200G SureFISH 13q34 LAMP1 598kb GR chr13:113699929-114297659 5MYB (6q23) del Copy number G101078R SureFISH 6q23 MYB 251kb RD chr6:135401418-135652368 5BCL3 Break apart G101103R SureFISH 19q13.31 BCL3 5' BA 305kb RD chr19:44928077-45232877 5

G101104G SureFISH 19q13.32 BCL3 3' BA 451kb GR chr19:45240362-45691197 5


RUNX1T1/RUNX1 (ETO/AML1) t(8;21)(q21;q22)





CEP9 Copy number G101069R SureFISH Chr9 CEP 367kb RD chr9:38079360-38446085 5CEP11 Copy number G101083G SureFISH Chr11 CEP 798kb chr11:47590964-48388829 5CEP15 Copy number G101072B SureFISH Chr15 CEP 265kb BL chr15:22822523-23087568 5

Dual fusion G110903G SureFISH 14q32.33 IGH DF 1519kb GR chr14:105767975-107286755 5G101118R SureFISH 4p16.3 FGFR3 DF 623kb RD chr4:1478716-2102191 5

CDKN2C(P18) 1p

Copy number G101227R SureFISH 1p32.3 CDKN2C 153kb RD chr1:51362027-51515472 5

CKS1B 1q Copy number G101159G SureFISH 1q21.3 CKS1B 386kb GR chr1:154735450-155121060 5PDGFRB (5q33) Break apart G101121R SureFISH 5q32 PDFGRB 3’ BA 354kb RD chr5:149148818-149502848 5

G101122G SureFISH 5q32 PDFGRB 5’ BA 435kb GR chr5:149507473-149942548 5Tri color G101125G SureFISH 4q12 FIP1L1 TC 500kb GR chr4:53767392-54267117 5

G101126R SureFISH 4q12 CHIC2 TC 469kb RD chr4:54475387-54944677 5G101127B SureFISH 4q12 PDGFRA TC 481kb BL chr4:55143797-55625077 5

7q del/D7S486 Copy number G101032R SureFISH 7q31.2 D7S486 200kb RD chr7:115794678-115994953 520q12 del Copy number G101033R SureFISH D20S108 20q12 200kb RD chr20:40730450-40930670 5CEP8 Copy number G101034R SureFISH Chr8 CEP RD chr8:48044527-48680019 55q33 del/CSF1R Copy number G101035R SureFISH 5q32 CSF1R 286kb RD chr5:149300503-149585908 55p D5S630 Copy number G101037G SureFISH 5p15.31 D5S630 370kb GR chr5:9400880-9768970 5RUNX1 (AML1) Break apart G101136R SureFISH 21q22.12 RUNX1 3’ BA 334kb RD chr21:35872349-36206704 5

G101137G SureFISH 21q22.12 RUNX1 5’ BA 573kb GR chr21:36231874-36804519 5NUP98 Break apart G101140R SureFISH 11p15.4 NUP98 3’ BA 132kb RD chr11:3624569-3756414 5

G101141G SureFISH 11p15.4 NUP98 5’ BA 416kb GR chr11:3774544-4190214 5FLT3 Break apart G101160R SureFISH 13q12.2 FLT3 3’ BA 428kb RD chr13:28167020-28595240 5

G101161G SureFISH 13q12.2 FLT3 5’ BA 496kb GR chr13:28614120-29110245 5

13q14 Copy number G101095G SureFISH 13q14.2 RB1 522kb GR chr13:48713435-49235090 521q22 Copy number G101096R SureFISH 21q22.13 DSCR8 494kb RD chr21:39307094-39800884 5CEP18 Copy number G101074B SureFISH Chr18 CEP 711kb BL chr18:18815593-19526958 5CEPX Copy number G101062G SureFISH ChrX CEP 587kb GR chrX:62492877-63079477 5CEPY Copy number G101063R SureFISH ChrY CEP 273kb RD chrY:21635490-21908125 5

Terc Copy number G100959R SureFISH 3q26 TERC 1014kb RD chr3:168975249-169989619 5Tert Copy number G100958G SureFISH 5p15.33 TERT 1107kb GR chr5:733225-1840695 5CEP7 Copy number G101099B SureFISH Chr7 CEP BL chr7:66804212-67571167 5

4q12 del (FIP1L1/CHIC2/PDGFRA)

IGH/FGFR3 t(4;14)(p16.3;q32.3)




CEP1 (R,G,B) Copy number G101064G SureFISH Chr1 CEP 541kb chr1:119400971-119941816 5CEP2 (R,G,B) Copy number G101089G SureFISH Chr2 CEP 448kb chr2:95620676-96068866 5CEP3 (R,G,B) Copy number G101065G SureFISH Chr3 CEP 592kb chr3:93675359-94267309 5CEP4 (R,G,B) Copy number G101066G SureFISH Chr4 CEP 613kb chr4:48100271-48712926 5CEP5 (R,G,B) Copy number G101067G SureFISH Chr5 CEP 633kb chr5:45247982-45881377 5CEP6 (R,G,B) Copy number G101068G SureFISH Chr6 CEP 706kb chr6:62316109-63021884 5CEP7 (R,G,B) Copy number G101099G SureFISH Chr7 CEP chr7:66804212-67571167 5CEP8 (R,G,B) Copy number G101034G SureFISH Chr8 CEP chr8:48044527-48680019 5CEP9 (R,G,B) Copy number G101069G SureFISH Chr9 CEP 367kb chr9:38079360-38446085 5CEP10 (R,G,B) Copy number G101084G SureFISH Chr10 CEP 676kb chr10:36335860-37011870 5CEP11 (R,G,B) Copy number G101083G SureFISH Chr11 CEP 798kb chr11:47590964-48388829 5CEP12 (R,G,B) Copy number G101090G SureFISH Chr12 CEP 704kb chr12:33231446-33935581 5CEP13 (R,G,B) Copy number G101070G SureFISH Chr13 CEP 454kb chr13:20618615-21072960 5CEP14 (R,G,B) Copy number G101071G SureFISH Chr14 CEP 536kb chr14:20754530-21290760 5CEP15 (R,G,B) Copy number G101072G SureFISH Chr15 CEP 265kb chr15:22822523-23087568 5CEP16 (R,G,B) Copy number G101073G SureFISH Chr16 CEP 671kb chr16:46529876-47200996 5CEP17 (R,G,B) Copy number G101105G SureFISH Chr17 CEP 436kb chr17:20892396-21327941 5CEP18 (R,G,B) Copy number G101074G SureFISH Chr18 CEP 711kb chr18:18815593-19526958 5CEP19 (R,G,B) Copy number G101075G SureFISH Chr19 CEP 527kb chr19:28377282-28904717 5CEP20 (R,G,B) Copy number G101086G SureFISH Chr20 CEP 687kb chr20:24929485-25616180 5CEP21 (R,G,B) Copy number G101087G SureFISH Chr21 CEP 653kb chr21:15498179-16151294 5CEP22 (R,G,B) Copy number G101088G SureFISH Chr22 CEP 668kb chr22:17525435-18193060 5CEPX (R,G,B) Copy number G101062G SureFISH ChrX CEP 587kb chrX:62492877-63079477 5CEPY (R,G,B) Copy number G101063G SureFISH ChrY CEP 273kb chrY:21635490-21908125 5



Information, descriptions, and specifications in this publication are subject to change without notice. Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material.

FOR MORE INFORMATIONLearn more or buy online: www.agilent.com/genomics/surefish www.dako.com

IQFISH Fast Hybridization Buffer 200

IQFISH Fast Hybridization Buffer 200x6

Dako Histology FISH Accessory Kit

Dako Cytology FISH Accessory Kit

FISH Hybridization Buffer (non-FFPE only)

Wash Buffer 1

Wash Buffer 2

Mounting Buffer with DAPI

Mounting Buffer without DAPI

FISH Hybridization Buffer and Mounting Buffer with DAPI

FISH Hybridization Buffer and Mounting Buffer with DAPI

G9415A

G9416A

K5799

K5499

G9400A

G9401A

G9402A

G9404A

G9403A

G9405A

G9406A

200 µL each

200 µL; 6 vials

kit (20 tests)

kit (20 tests)

100 µL/vial

500 mL/bottle

500 mL/bottle

100 µL/vial

100 µL/vial

100 µL each

300 µL each

Product Name Catalog ID Kit Size

Ancillary Reagents

For Laboratory Use Only

© Agilent Technologies, Inc. 2015 Printed in USA, August 17, 2015 Pub No: 5991-4649ENLit No: 00034 2015AUG17

Documents

—The Next Generation FISH Technologykromat.hu/UserFiles/files/patologia/SureFISH_Catalog_US-12688.pdfA 50 kb deletion within the IKZF1 gene is clinically important in leukemia. A