For reseach use only. Not for use in diagnostic procedures.

Automate Your Roche® and Kapa® Library Preparation

Are you looking for a solution to increase the throughput of your Kapa Biosystems® or Roche® library preparation, while decreasing hands-on-time and minimizing variability and sample tracking errors? PerkinElmer’s portfolio of dedicated liquid handling workstations offer walkaway automation of KAPA® and Roche® library preparation chemistries (Table 1) with the flexibility to meet your laboratory’s needs.

Applications include:

• Whole genome/exome sequencing• Targeted DNA/RNA sequencing• Gene expression analysis• Identification of novel transcripts• Detection of alternative splicing and fusion transcripts• Isoform detection

Automated Library Preparation with Throughput Flexibility to Match Your Workflow

PerkinElmer offers true walkaway library prep with easy-to-use, proven methods for nearly all sequencing platforms.

The Sciclone® G3 NGSx Workstation is the high-throughput solution for library prep, target enrichment, and normalization with the ability to construct up to or greater than 96 libraries per day.

The JANUS® G3R NGS Express Workstation uses an intuitive user interface for automating lower throughput applications such as, NGS library preparation, amplicon-based library preparation, target enrichment and normalization. Highly visible status light indicators provide confirmation and confidence enabling walkaway automated sample preparation.

The Zephyr® G3 NGS Workstation is a benchtop liquid handler designed to address medium-throughput automated library preparation workflows. The workstation offers a walkaway solution with flexible processing enabling the parallel processing of 8-48 NGS library or exome preparations or a 96-well plate of SPRI size selections / post-PCR clean-ups per run.

Increase Your Throughput of Kapa® Biosystems & Roche® Library Preparation

CHEMISTRIES Sciclone® JANUS® Zephyr®

KAPA® DNA HTP Library Prep Kit

KAPA® DNA HyperPrep™ Kit

KAPA® DNA HyperPlus Kit

KAPA® RNA HyperPrep™ Kit

KAPA® RNA HyperPrep™ with

RiboErase™ Kit

KAPA® mRNA HyperPrep Kit

KAPA® Stranded RNA-Seq Kit

KAPA® Stranded RNA-Seq with

RiboErase™ Kit

KAPA® Stranded mRNA-Seq Kit

Roche® SeqCap® EZ Kit

Roche® SeqCap® EZ HyperCap Kit *

KAPA® Library Quant Kit

KAPA® hgDNA Quantification

and QC Kit

KAPA® SYBR® FAST qPCR Kit

developed * in development

PerkinElmer develops applications for automated NGS sample processing based on chemistries developed by Kapa® Biosystems.

Table 1. PerkinElmer ‘walkaway’sample prep methods for KAPA chemistries

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Eliminate Library Preparation Bottlenecks

PerkinElmer collaborates with leading library prep kit vendors to deliver robust automation solutions for the protocols you are using today or may need in the future. With over 85 of the most frequently used library prep protocols currently programmed on our NGS workstations, you can have your lab’s library prep automated in as little as a week.

Sciclone G3 NGS / NGSx Workstation

Sample Capacity:

• Up to or greater than 96 libraries per day

Features:

• Recessed deck for additional tip storage capacity

• 96-channel head for efficient pipetting of reagents

• Error recovery procedure

• Ability to load a partial box of tips

Ideal for:

High-throughput library preparation

JANUS G3R NGS Express Workstation

Sample Capacity:

• 8-24 samples per day

Features:

• VersaTip® technology enables dual-capability of a fixed cannula and the use of disposable tips

• Tube-to-plate dispense capability

• Error recovery procedure

• Accusense™ patented independent liquid-level sensing technology

Ideal for:

Benchtop NGS with low-to-moderate throughput capacity

Zephyr G3 NGS Workstation

Sample Capacity:

• 8-96 samples per day

Features:

• Compact benchtop footprint

• 96-channel head for efficient pipetting of reagents

• Error recovery procedure

• Ability to load a partial box of tips

Ideal for:

Medium-throughput library preparation; pre- and post-PCR separation

AUTOMATED NGS LIBRARY PREPARATION WORKSTATIONS

NEW: Optional UV light and On Deck Thermal Cycler-ODTC® 96 and 384 instruments (inheco®) are available for for Sciclone and JANUS Automated Liquid Handling Workstations.