TB‐dx Final Design

J. Bentley, K. Cooley, D. Crowe, M. Evans, L. Sehgal

University of Rochester

Department of Biomedical Engineering

05/06/2009

Compiled for Benjamin Castaneda and Fanny Casado by Automated

Diagnostics under the supervision of Edward Brown III, Ph.D.

Outline Problem Statement

Customer Scenario

Standard of Care in Peru

Overall System Design

Division of Tasks

Design Process

Final Design

Future Prospects

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The Issue

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Medicine World

Customer Scenario Hospital Nacional Dos de Mayo

in Lima, Peru

Current Situation in Peru

TB is a serious public health threat in Peru

44% of the population is below the poverty line

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Standard of Health Care in Peru Tuberculosis Diagnostic Procedure

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Image source: PUCPDigital image of Bacilloscopy

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Overall System Design

TB-dx Setup NEEDS NEW PIC!!!

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User Interface Quick Summary of user interface…

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Pontificia Universidad Catolica del Peru Image processing and

detection of mycobacteria

Count bacilli

Future: Auto Focusing

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Image Processing Team from PUCP

University of Rochester

Receive output # of bacilli from PUCP

Automate stage movement for 1 slide

Quantify/qualify focus problems

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Automated Diagnostics

Experimental Results Conducted experiments to determine the sensitivity of

focus across a slide with 100X oil lens

Used parallel slices of rat femur and uneven human TB-free sputum samples to quantify

Attempted to move as many millimeters as possible until the sample was out of focus

On average, human samples had to be re-focused every millimeter

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Prototype Iteration #1

Flat slide bed Rubber band belts

Plastic motor housing

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Prototype Iteration #2

Multi-layered slide bed

Rubber band belts

Machined aluminum motor housing

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Final Design: TB-dx

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Final Design: TB-dx

Existing slide holder on Olympus CX31 microscope stage

Flat NBR‐Coated Nylon Fabric Belts

Machined aluminum motor housing

NEED PICTURE WITH NEW BELTS HERE

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Olympus CX31 Manual

Future Prospects Multiple Slides

Automated Focus

Compatibility

Zeiss

Computer Free Product

Entire System Testing

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Carl Zeiss’ Primo Star iLED fluorescence microscope

Thank You

• Ben Castaneda

• Fanny Casado

• Professor Ed Brown

•

• Art Salo

• Paul Osborne

• Professor Scott Seidman

• Professor Amy Lerner

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