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••• foreword

nutraceuticals now 03

At the upcoming Vitafoods Asia exhibition, you can see exactly how the food, supplement, beauty and pharmaceutical industries are being transformed by innovators and scientists who are working to deliver optimal health in what you consume.

The exhibition is a full-service look into the nutraceuticals industry, from its key ingredients, to contract manufacturing and labelling to services & equipment and branded finished products. Along with what is being offered commercially, there’s much opportunity to discover what is also in development. For example, how modern medicine is being sourced and developed from natural ingredients.Bringing innovation and invention to the forefrontVitafoods Asia brings together manufacturers at the forefront of innovation and invention in the industry to meet and discuss their product development challenges. Visitors that attend Vitafoods Asia can meet and source from suppliers of the very latest products hitting the market.

White-label and private-label products ready for market products are a quick and cost-effective way to access and take advantage of rapid market growth. Product demand is driven by consumers becoming increasingly aware of preventive medicines and the positive link between diet and disease

prevention. Modor Intelligence reports1 that collectively, Japan and increasingly China, India, Australia, Korea, Thailand and Vietnam, are growing the nutraceuticals market in Asia-Pacific by 7.5% year on year.

In 2018, Grand View Research reported2 that the global nutraceutical market could be valued at $578.23 billion by the year 2025. To take advantage of this market opportunity, distributors, retailers and manufacturers can discover and source high-quality, innovative dietary supplements and functional food and beverages all under one roof at Vitafoods Asia.

At Vitafoods Asia you can source and taste the latest innovative products that help improve health, wellbeing, physical and mental health, from energy drinks to meal replacement bars, chewable supplements to effervescent tablets and everything in-between. Bespoke Manufacturing and Private LabellingThe exhibition is an easy place to source white-label and private-label products that are ready for market. Such products are a quick and cost-effective way to access and take advantage of rapid market growth.

Moreover, buyers can meet with manufacturers that create bespoke products. Whether it’s a new extract, vitamin, mineral or protein, energy bar, isotonic sports drink or enriched

How science is shaping the future of nutritious consumablesWhat if you didn’t have to diet or take supplements? What if you could trust that what you consume is designed to keep you in optimum health instead? Well, that’s almost a reality. Ironically, it’s our propensity for a sedentary lifestyle and fast food that’s helping to drive demand for healthier products to consume, and thus a bourgeoning research industry in support of nutritious foods and ingredients, beverages, dietary supplements, nutricosmetics and personal care.

In an industry as dynamic and innovative as ours, it’s hardly surprising there’s plenty to look forward to at Vitafoods Asia 2018!

The nutraceutical and functional food industry is in an exciting phase—we’re seeing collaboration across sectors to create innovative solutions to consumer health concerns, and together, we stand on the precipice of the next health and wellness revolutions. And collaboration is what we all need right now—we need to share insights and team up to continue shaping the industry we’re in.

Vitafoods Asia is proud to be the place where the industry meets to learn and network in this global, collaborative industry—in response to growing demand for nutraceuticals in functional foods and beverages. We serve as a platform for more than 300 exhibiting companies from around the world. You’ll find innovation in action in all corners of the exhibition. As the only dedicated event in Asia, we’re shaping the industry through connecting the entire supply chain across four key industry sectors: ingredients, finished products, contract manufacturing and services. There’s expertise, insight, innovation, and something for everyone. Spotlighting Omega-3 and ProbioticsWith increasing demand from China, Southeast Asia and other developing countries for Omega 3 means that the market for the most widely used nutritional lipid continues to grow as it controls inflammation. GOED estimates the market today globally is worth US$1.5 billion at the ingredient level. Learn more at the Omega-3 Resource Centre and Tasting Centre on the exhibition floor.

The International Probiotics Association (IPA) suggests that the market for probiotics is experiencing unprecedented growth

and may be worth as much as US$43 billion. To showcase the latest breakthroughs in probiotic technology, to new areas of product development and consumer analysis, the organisers of Vitafoods Asia are introducing a new resource, the Probiotic Resource Centre, for the first time in Asia.Addressing Asia’s ageing populationsCurrently home to 60% of the world’s 60 years or older population, Asia’s ageing population is expected to more than double from 547 million in 2016 to nearly 1.3 billion by 2050 .

Vitafoods Asia’s Life Stages Theatre addresses matters of healthy ageing by delivering expertise on the specific nutrition requirements that are needed from infancy until later in life. It also includes sessions on regulatory guidance and marketing strategies as they relate to specific life stages.

At the Global Health Theatre at Vitafoods Asia, the impact of the modern lifestyle on nutrition and diseases, and how food fortification is addressing these issues, will be discussed by industry experts. Responsible nutrition, importance of fortification & fortified foods, impact on the glycaemic index, as well as the packaging of nutraceuticals into functional foods, will be discussed, among other topics.

Attending Vitafoods Asia is also an essential opportunity to connect and learn from members across the entire nutraceutical supply chain spectrum. A dedicated area, the Industry Advice Zone, is available for visitors to meet with industry experts in one to one consultation sessions, for regulatory advice, market access information and strategies, and marketing and product innovation.

As there’s so much to discover at Vitafoods Asia 2018, I hope this will give you a head start on planning your visit.

I look forward to seeing you there!

By Chris Lee, Managing Director, Health and Nutrition, Europe

04 nutraceuticals now nutraceuticals now 05

gummies, buyers can source for manufacturers to develop new products specific to their needs.

Similarly, if you need help with technical manufacturing processes or to deal with ever-increasing regulatory complexities, you will be able to schedule one-on-one consultation sessions with specialists in various fields at the Industry Advice Zone to discuss regulatory advice, market access information and strategies, and marketing and innovation profiling. Discover a wide-range of service and equipment providers that are dedicated to the nutraceutical industry and help brands to develop products that deliver optimal nutrition.Shaping nutrition for optimal sports performance An example of a growing market is that demand for sports nutrition is rising as comprehension improves as to the value of nutrition among athletes and gym professionals. This is especially true of markets in China, India and Italy.

Today, functional foods and beverages that contain ingredients such proteins, vitamins, amino acids and omega-3 fatty acids to help attain peak performance are readily available. Along with these ingredients, minerals and herbs are being used in functional beverages to help keep blood sugar levels at optimal levels is another fast-emerging market.

Cosimo Palumbo, the Marketing Director for IDENA S.p.A., says that they continue participate in Vitafoods Asia because, “although we have been participating since the very first few

editions, lately we’ve found that the show has grown both in number and quality of exhibitors, visitors, and scientific program. We expect the same high quality also this year and onward.”

“This year, IDENA is leveraging the New Products Zone at Vitafoods Asia to launch two new products. Firstly, VazguardTM, a Bergamot Phytosome®, which uses a highly standardized bergamot polyphenols fraction that is formulated to optimize the biological absorption of bergamot polyphenols. VazguardTM is safe and effective for the reduction of cardiovascular risk by modulating total cholesterol (tChol), low-density Lipoproteins (LDL), high-density Lipoproteins (HDL), tryglicerides (TG) and blood glucose.

The other product is QuercefitTM, a new natural boost for sports nutrition. Generally, there is poor availability of quercetin, and so we are offering quercetin with the Phytosome® delivery system, which helps to improve

plasmatic levels of this natural botanical active. This was proven in a pharmacokinetic study in humans. At Vitafoods Asia, we will put forward the clinical data available that demonstrates evidence of activity in conditions characterized by an abnormal oxidative stress like intensive training, improving an athlete’s performance and aiding in recovery.”

Ingredient innovations that are driving the future of the industry are featured in production demonstration display areas and new functional food and drinks can be sampled at the Tasting Centre.The world’s most impactful brands are represented The world’s most notable developers of food and beverages are represented at the Vitafoods Asia exhibition, for example, DuPont Nutrition & Health, who manufacture emulsifiers and sweeteners, probiotics, cultures and food protection ingredients; protein solutions; systems and texturants; specialty hydrocolloids; pharma excipients.

Dr. Li Yongjing, Regional President, Asia, DuPont Nutrition & Health, says “DuPont Nutrition & Health is a world leader in applying market-driven science and innovation. This year, we want to place products with health-enhancing benefits and solutions backed by groundbreaking science at the heart of our presence at Vitafoods Asia. DuPont will demonstrate excellence in demand-driven health ingredients solutions. We are also committed to sharing the research behind and

we will demonstrate how our science can be translated into successful ingredient formulations and product launches at our hospitality suite. We welcome everyone to visit us at the DuPont Hospitality Suite, Angsana 3A & 3B, where our team of industry experts will be present to help turn market challenges into business opportunities.”

Registration is now open to Vitafoods Asia. Connect with over 5,500 visitors from 60 countries and explore the more than 300 exhibitors of key ingredients, branded, finished goods, medicines, nutricosmetics, personal care and animal nutrition.

Visit www.vitafoodsasia.com to register for free.

References1 Asia-Pacific Nutraceutical Market - Growth, Trends and Forecasts (2018 - 2023)2 Nutraceuticals Market Size Worth $578.23 Billion By 2025 | CAGR: 8.8%

Fi India 30 August – 1 September 2018, India Expo Centre, Greater Noida, Delhi-NCR, India.

Nat expo 23/24 September 2018.Lyon, France

Vitafoods Asia11-12 September 2018. Sands Expo. Singapore.

Supply Side West 2018 November 6-10, 2018 Expo Hall November 8 & 9 Mandalay Bay, 3950 S Las Vegas Blvd, Las Vegas, U.S.A

Hi Europe 27-29th November 2018Frankfurt, Germany

Food Matter Live 20-22nd November 2018London, Excel. UK

Biofach13-16 February 2019Nuremberg, Germany

Nutraceuticals Europe27-29th February 2019Madrid, Spain

2018/2019 Nutraceuticals Now

Natural products expo 5-9 March 2019Engredea – Anaheim, U.S.A

Supply Side East 9/10th April 2019Secaucus, NJ, U.S.A

Natural Organics 7/8th April 2019Excel, London.

Vitafoods 7-9th May 2019Geneva, Switzerland.

Free from Expo 28-29th May 2019Barcelona, Spain

Nutrevent June 2019Lille, France

Fi 19th-21st June 2019Shanghai, China.

IFT2-6th June 2019New Orleans U.SA

Events

contents

Nutraceuticals Now is a technical review providing the latest information on functional products and ingredients which are defined as having a disease preventing and/or health promoting benefit in addition to their nutritional value.It is targeted at manufacturers of food and drink, who are producing finished products aimed at the ever increasingly health conscious consumer.The opinions expressed in our published works are those of the author(s) and do not reflect the opinions of Johnson-Johnsen Publishing or its Editors.

Information contained in our published works have been obtained by Johnson-Johnsen from sources believed to be reliable. However, neither Johnson-Johnsen nor its authors guarantees the accuracy or completeness of any information published herein and neither Johnson-Johnsen nor its authors shall be responsible for any errors, omissions, or claims for damages, including exemplary damages, arising out of the use of or inability to use information, or with regard to the accuracy or sufficiency of the information contained in this publication.

All rights reserved. No part of any Johnson-Johnsen published work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without written permission from the publisher.

ISSN -1478-6605

Acknowledgement disclaimer from IFT Preview issue 2018.The publisher would like to correct positioning of articles from the 2018 IFT preview issue of Nutraceuticals now. Dr Dash’s statement “ Probiotics are more important than multivitamins and should be taken daily” has no

associationwith company Sabinsa, and that Dr Dash has no assocation with nor endorses any other Probiotic

company.

Editor: Charles FaulknerOperations Manager: Dan PeacockConsultant: Christopher McIntyreAccounts: Steve ClarkDesigner: Al KnightPublished by: Johnson-Johnsen Publishing 6 Victoria Terrace, Inverness, IV2 3QA, Scotland.Tel: +44(0) 1463 718993Fax: +44(0) 1463 229976Email: info@nutraceuticalsnow.comWebsite: www.nutraceuticalsnow.comPrinted by: Harrisons

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advertisers07 Horphag09 FoodMatters Live15 Vidya Europe 21 NatExpo27 Jennewein39 Fi india

39 IFIS45 VitaFoods Asia46 Hi Europe47 Sabinsa48 Lallemand

02 Dr Dash03-04 Foreword05 2018/19 Nutraceuticals Now events08 Discover the nutraceuticals industry at Vitafoods Asia10-11 UAS Labs - a longstanding leader in the probiotic industry12-14 Care your skin with SkinCera™ 16-17 Sepibliss™ - natural and innovative solution to comfort sensitive skin orally18 Frutarom enters “Beauty from Within” market in Asia19-20 Probiotics can boost sports nutrition21 Vitafoods Company News22-23 Alternative carb opens up new sports nutrition opportunities24-25 Bridging the fibre gap26-28 Human milk oligosaccharides, not only for breast fed babies29 Prodiet fluid30-31 EnoSTIM™, the natural male enhancer32 Gelita AG joins the animal welfare initiative as a sponsoring member33 Fruit d’Or Company News34-35 Research-backed ingredients provide product differentiation in crowded sports nutrition market36-37 Use of clean ingredients to help meet consumer demands and government targets38 Hi europe Company News40-41 Don’t let aggressive formulations affect your tablet production42-43 From epidemiological studies to a patented and award ingredient44 The fight against diabesity

08 nutraceuticals now

••• preview

XX July 2018, Singapore – Visitors to the upcoming Vitafoods Asia Exhibition and Conference in Singapore, 11-12 September 2018, will discover a rich learning environment to discover all aspects of the development and application of nutraceuticals, through to product positioning, branding and marketing, inclusive of how to overcome regulatory challenges in various markets.

Health care spending in the Asia-Pacific region is anticipated to double by 2050, opening opportunities to market alternative remedies and fortified foods. Across Asia, and especially in China and India, increased disposable income, coupled with ageing populations, are impacting the prevalence of lifestyle-related diseases, along with blood pressure, diabetes, or cardiac diseases. Inadequate nutrition, due to busy lifestyles, and the high cost of healthcare are also factors. Joint health, gut health, bone health and weight management are expected to be future demand drivers. Functional FoodsAsia Pacific is now the largest regional market for dietary supplements and vitamins. Malaysia and India for instance, are grappling with the impact of rapid urbanisation on the diet. Demand functional, hearty-healthy foods, for example, is rising.

Aside from products developed in Western societies, across the region, consumers are buying products that are enriched with locally-sourced compounds and functional ingredients like probiotics, fibre, calcium or vitamin E; herbs such as tongkat ali and ginseng; along with cultured milk drinks; probiotic yogurts; and cereals that are fortified with fibre; plus, Omega-3 fortified eggs.

Vitafoods Asia showcases key suppliers of such ingredients and raw materials that are used in functional foods. Alongside these suppliers, visitors to the exhibition can source contract manufacturers and private labellers, as well as finished goods manufacturers.Functional BeveragesTo tackle alertness and reduce calorie consumption, as well as hydrate with performance additives, athletes and gym enthusiasts in countries like China and India are driving demand for nutrition through functional beverages. Energy drinks, sports drinks & nutraceutical drinks are the fastest growing segment of the functional beverage market. Due to its efficient delivery of minerals or herbs, functional beverages are emerging to help reduce the risk of chronic disease and to keep blood sugar levels under control.

Drinks ingredients include vitamins, amino acids and omega-3 fatty acids. Amino acids are used to slow fatigue and vitamins are added to boost the metabolism and generate energy in functional beverages. Prebiotics and probiotics are also used to ensure proper functioning of the digestive system. Another ingredient, Omega-3, is forecast to grow in the functional beverages market as it controls inflammation. At Vitafoods Asia, there’s an Omega-3 Resource Centre where visitors can witness the latest innovations and product development initiatives around Omega-3.

Manufacturers in this market segment are developing innovative flavours to appeal to consumers with drinks that are safe, taste good and are affordable. Key functional beverage manufacturers are exhibiting at the Vitafoods Asia Exhibition.

Addressing MalnutritionIn countries like Japan and Thailand, where despite relatively healthy diets, there are mineral and vitamin deficiencies in the population. In Thailand, Calcium and Vitamin D are issues, and doctors are prescribing supplements. The Japanese lack calcium and iron, so there is a corresponding seeing rise in demand for probiotic yogurts and energy drinks.

At the Global Health Theatre at Vitafoods Asia, the impact of the modern lifestyle on nutrition and diseases, and how food fortification is addressing these issues, will be discussed industry experts. Responsible nutrition, importance of fortification & fortified foods, impact on the glycaemic index, as well as the packaging of nutraceuticals into functional foods, will be discussed, among other topics. Food fortificationAs scientific research evolves around issues such as obesity and disease, as the impact of nutrition on pre-natal and maternal life stages, sports and aging evolves, the way we approach these issues is changing. You can stay abreast of the latest research by attending the Life Stages Theatre at Vitafoods Asia, where you will hear from researchers and functional food developers alike. Processing food to protect bioactive ingredientsAt the Vitafoods Asia exhibition, visitors will learn how processes such as microencapsulation, enzyme technology, and nanoencapsulation of ingredients are being used to produce functional foods. Of late, microencapsulation technology has been developed to replace cyclodextrin (CD) molecules to protect the bioactive elements in food processing through to the storage and delivery of functional foods.

Notably, product formulation for the delivery of nutraceuticals is changing. For example, Indian consumers are driving change from traditional tablets, or chewable tablets, to capsules, particularly liquid-filled capsules, which are easier to swallow and are believed to work faster and better. At Vitafoods Asia, discover novel formulations that allow nutraceutical companies to incorporate liquids, pellets, tablets and powders in capsules.

Obtaining the right advice Attending Vitafoods Asia is an essential opportunity

to connect and learn from members across the entire nutraceutical supply chain spectrum, from leading manufacturers and distributors to buyers and quality suppliers. A dedicated area, the Industry Advice Zone, is available for visitors to meet with industry experts in one to one consultation sessions, for regulatory advice, market access information and strategies, and marketing and innovation profiling.

The Vitafoods Asia Conference is another chance to learn from academia, government and industry leaders. Structured to help participants maximise their learning in line with their career interests, dedicated conference tracks will guide participants towards talks on research and development, business and marketing strategies, and digestive health. Sessions also address challenges to do with market entry and regulation.

Discover the Nutraceuticals Industry at Vitafoods AsiaVitafoods Asia is a key regional learning event centred on the latest research, development and application of nutraceuticals into functional foods and beverages; as well as the marketing, branding and regulations of such fortified products

10 nutraceuticals now nutraceuticals now 11

by the fully-integrated nature of UAS Labs, which enables it to maintain standards from the production of raw materials through to shipping finished products.

While they have made large investments in their physical facilities the real investment was made with probiotic partners and end-consumers in mind. UAS Labs will use their probiotic-only manufacturing facilities to drive their mission of providing leading and trusted probiotic-based solutions through the creative application of science, quality and talent in mind.Overcoming formulation challengesUAS Labs, a provider of private label, contract manufacturing and raw materials services, has paired its production capabilities with substantial R&D and quality control operations. This combination yields benefits for UAS Labs and its customers.

If a contract manufacturing organization (CMO) comes to UAS Labs with a recipe and ambition to turn it into a product, the R&D team will assess various aspects of the formulation. Sometimes the formulation needs work if it is to be commercially viable, and this is when working with a partner with expertise and proprietary technologies pays dividends.

UAS Labs is particularly well equipped to manage the water activity of secondary materials. While the characteristics of the probiotic are set in stone, the optimization of the secondary materials used in the final product provide an opportunity to increase the viability of the formulation.

Recognizing this, UAS Labs has developed proprietary technologies to reduce the water activity of materials that would otherwise fail to meet its requirements. This permits the use of a wider range of materials than would otherwise be possible.

Once UAS Labs has a formulation that looks viable, it makes a small batch for use in stability tests. These tests use UAS Labs’ stability-assessment chambers, another resource in which the firm has invested. UAS Labs now has six stability-testing chambers. Products UAS Labs makes go into the chambers and are tested monthly or quarterly to gauge their long-viability. This way, UAS Labs can continuously review and improve the shelf life of its products.Clinical trials and documented strainsThe focus on scientific excellence that characterizes UAS Labs’ R&D team stems, in part, from their CSO. Dr Leyer is a primary researcher who specializes in probiotic efficacy and applications. He directs the R&D laboratory and UAS Labs’ clinical trial activities. The capabilities of the R&D laboratory and the very existence of the clinical trial program are testament to the commitment of UAS Labs to the advancement of scientific understanding of probiotics.

Few manufacturers conduct gold-standard clinical trials of probiotic strains and finished formulations. UAS Labs is part of this elite group. The company’s investment in clinical trials reflects its desire to further scientific understanding of probiotics and its belief that data from rigorous human studies will become increasingly important to ensure customers are receiving the best possible products backed by rigorous science.

The goal of UAS Labs’ study program is to put a substantial portion of private label formulations through double-blind, placebo-controlled randomized clinical trials. In doing so, it will generate data on finished product formulations. As UAS Labs does business in over 45 countries, it operates in markets where such data is needed today.

UAS Labs has a track record of successfully running clinical studies as shown with three of their trademarked superstrains:

• Lactobacillus reuteri NCIMB 30242 (LRC™) is the world’s most effective and clinically documented heart health probiotic strain

• Lactobacillus acidophilus DDS®-1 is arguably the best described probiotic species with over four decades of clinical research

• Lactobacillus gasseri BNR17™ is a weight management strain that has been put through extensive pre-clinical analysis and it is the subject of three published human clinical trials

These superstrains, exclusively offered by UAS Labs, are recognized throughout the industry for their science. In fact, Lactobacillus gasseri BNR17™ was just award Weight Management Ingredient of the Year, 2018 based on its scientific data and ability to meet a market demand.

With nearly 40 years in the probiotic industry it would be easy for UAS Labs to rely on longevity for success, but that is not the case! UAS continues to use their experience and expertise to advance the field of probiotics. They adapt operations to the ever-changing landscape and remain at the forefront of clinical advancement.

Today, with expertise, science and state-of-the-art facilities in place, UAS Labs is as well-equipped as ever to continue breaking new ground in probiotics and meeting the needs of their strategic partners.

In recent years, notably since new owners took over the company in 2013, UAS Labs’ commitment to pioneering practices has led them to make outsized investments. These investments include growth and process improvements in their Wausau, Wisconsin, USA manufacturing plant, the acquisition of another probiotic manufacturer, the opening of their new, state-of-the art fermentation facility, licensing rights to clinically proven strains and ongoing clinical research. These investments have bolstered UAS Labs’ status as a producer of premium probiotics with deep in-house expertise and proprietary R&D technologies — a type of company that is almost as rare today as it was in 1979.Investing in manufacturing excellenceUAS Labs, also known as The Probiotic Company, is built upon its probiotic-only manufacturing facilities. Since UAS Labs’ CEO Kevin Mehring and CSO Dr Greg Leyer took charge of the company in 2013, they have made a considerable investment to ensure the stability and viability of their probiotic solutions.

Most recently UAS Labs announced a 60-million-dollar investment with the opening of their new fermentation facility in Madison, Wisconsin, USA. This investment was made to support their continuous growth and ongoing leadership

position in the probiotic marketplace. As true pioneers in the industry, the team at UAS identified the opportunity for a fermentation manufacturer that was able to successfully commercialize a growing list of probiotics – both traditional species as well as strains recently discovered by their global partners. Pairing their team of probiotic experts, with a facility engineered for product purity, UAS has the capacity to not only produce these strains commercially but also improve on standard industry lead-times.

Like the fermentation facility, UAS has a 45,000-square-foot facility in Wausau, Wisconsin, USA dedicated solely to the manufacturing of probiotic finished goods. This facility is yet another testament to UAS Labs’ focus on probiotics. Whereas mixed-use sites need to cater to the needs of a range of types of products, every design and process decision at UAS Labs’ facilities are made to best serve the unique characteristics of probiotics. As live microorganisms, each with their own requirements throughout production and distribution, probiotics place particular pressures on manufacturers. UAS Labs’ investment priorities demonstrate its commitment to ensuring optimal handling of these microorganisms.

At each step in the process from fermentation to distribution, UAS Labs seeks to create the optimal environment for probiotic viability. This degree of end-to-end control is made possible

UAS Labs has a long, important pedigree in probiotics. Founded in 1979 by Chairman Dr. Dash, the producer of premium probiotics has been at the forefront of the industry for almost as long as the industry has existed. Today, with equity partners and new management, UAS Laboratories, LLC has expanded its operations, added a probiotic fermentation plant, acquired Micropharma and Nebraska Culture, and added new probioic strains and products. It continues to

break new ground through its state-of-the-art manufacturing facilities, gold standard clinical trials, proven probiotic strains and development of condition-specific probiotics.

a longstanding leader in the probiotic industry

12 nutraceuticals now nutraceuticals now 13

The effect of time on skin healthAging is a complex, multi-factorial physiological process that affects all organs, but changes to the skin remains the most visible mark of passing time. The skin also reflects health and well-being. Along the years, as one gets older the skin loses its elasticity, forms wrinkles and pigmented lesions start appearing. But more than a cosmetic issue, skin aging and poor skin health may expose the body to significant health risks, since the skin is the first line defence from external forces. Skin protects our body from UV radiations, infectious microorganisms, and mechanical and chemical stress.1 Healthy skin is also vital to regulate body temperature, synthesize optimal amounts of vitamin D and provide critical sensory input from the environment.

The human skin consists of epidermis, corium and tela subcutanea. The epidermis is made of four layers, namely stratum corneum, stratum granulosum, stratum spinosum and stratum basale. With passage of time, studies show that the moisture content of the stratum corneum (epidermal layer) decreases.2 In the aged population, the stratum corneum is also susceptible to inflammation and infection. In vivo and human studies show that aged skin suffers alterations in epidermal integrity and recovery after experimental perturbations.3 This reduction of structural integrity is partly explained by a decrease in lipid content of aged skin, up to 32 % compared to young skins.3,4 Skin lipids are primarily composed of ceramides, fatty acids and cholesterol, and the synthesis of these vital lipids gradually decrease with age.3

Why does our ageing skin need additional ceramides?Ceramides represent about 40-50 % of stratum corneum and are formed from a sphingosine backbone and a fatty acid (Figure 1). They are key constituents of epidermal membrane and contribute to the maintenance and integrity of the permeability barrier.1 Together with cholesterol and saturated fatty acids, ceramides create a water-impermeable, protective structure to prevent excessive water loss due to evaporation as well as a barrier against the entry of external entities such as microorganisms. Ceramides and their metabolites are also involved in the modulation of key cellular functions such as proliferation, differentiation and apoptosis in epidermal keratinocyte cells.1

Quantity of ceramides is lower in the stratum corneum of aged individuals, and patients with atopic dermatitis and dry skin.4,5,6 The forearm skin of the aged (especially those over 70 years old) is usually xerotic, due to a decrease in ceramide content which is associated with dry appearance. In addition, comparison of total ceramides content of forearm stratum corneum between patient with atopic dermatitis and healthy subjects shows that in atopic dermatitis, there is a marked reduction in the amount of total ceramides in both lesional and nonlesional forearm skin.5 Levels of ceramides 1 and 3 are particularly lower in atopic dermatitis.6 In general, ceramides deficiency is observed in a number of skin pathologies such as psoriasis, eczema, atopic dermatitis, ichthyosis, xerosis and acne.7 This result suggested that ceramide is a key factor for moisture maintenance and barrier function of stratum corneum. Fine lines and wrinkles appear when ceramide content is reduced.

Figure 1: Generic structure of ceramides

Supplementation with ceramidesStudies confirm the gradual decline of the ceramides content in human

skin due to aging, possibly due to a decline in enzyme activity that helps to deliver ceramides in usable form to the skin. When aging skin’s ability to make ceramides diminishes, as it inevitably does, increased ceramides intake becomes necessary. Several studies

showed that supplementation with ceramides may improve deteriorated skin conditions. Indeed, topical applications of stratum corneum lipids and ceramides were reported to restore skin barrier, to alleviate dry skin and to repair skin.7 Clinical studies reported the usefulness of ceramides

supplementation in treating atopic dermatitis and eczema.Ceramides, naturally present in animal skin, are also

produced by some plants, and vegetal ceramides were also reported for their benefits on skin health. For example, in a RCT

trial involving 51 women aged 20-63 years, a significant increase in skin hydration and an improvement in associated clinical signs were

observed in women with dry skin after 3 months treatment with a wheat extract rich in ceramides.8 Another clinical study on 35 women showed that oral

Care your skin with SkinCeraTM

. . . a patented konjac extract by Vidya HerbsBy Aurélie Leverrier, R&D Manager Vidya Europe

intake of beet ceramides improves skin elasticity in a dose dependent manner and may stimulate intracellular signals and exert favourable effects on the extracellular matrix, such as induction of fibronectin synthesis.9 Administration of glucosylceramides to mice was also proven to be useful in reduction of transepidermal water loss and for improvement of skin barrier function by up-regulating genes associated with the cornified envelope and tight junction formation.10,11

SkinCeraTM, a patented konjac extract standardized for ceramides by Vidya HerbsKonjac (Amorphophallus konjac) is a plant from Araceae family native to a wide region from warm subtropical to tropical eastern Asia, where its flour is widely used as a food source. In China, it has been used for more than 2000 years in the treatment of asthma, cough, hernia, breast pain, burns as well as haematological and skin disorders.12 Konjac is rich in polysaccharides, namely glucomannans, and is reported to have anti-obesity, anti-hyperglycaemic, anti-hypercholesterolemia activities, laxative effects, and prebiotic and anti-inflammatory activities.11

Konjac glucomannan hydrolysates are also reported to improve skin health by promoting the topical immune response and collagen regrowth at cuts, and by preventing and protecting against infections, such as P. acnes.13 A clinical study reported the benefits of konjac formulation containing 5 % glucomannans on acne vulgaris and skin health.14 Besides, the intake of 1.8 mg/

day of konjac ceramides by children with moderate atopic dermatitis improves skin symptoms and allergic responses.15 Oral intake of konjac glucosylceramides is also reported to improve the level of transepidermal water loss in mice and health human subjects.16

SkinCeraTM by Vidya Herbs is an ethanolic extract of konjac rhizomes standardized for 5, 10 or 20 % glucosylceramides, among them ceramides 1, 2, 3 and 4 are the major compounds (Figure 2). SkinCeraTM is a potent nutricosmetic product useful to maintain skin health and the skin lipid barrier, for which a patent was deposited. In in-vitro and in-silico studies, SkinCeraTM showed moisturizing effects and was proven to fight the signs of aging, by the inhibition of 3 key enzymes: tyrosinase, elastase and collagenase.

Anti-tyrosinase, anti-elastase, anti-collagenase and moisturizing effects of SkinCeraTM

Tyrosinase is a copper containing oxidase found in melanocytes, and is a key enzyme in melanin biosynthesis, a pigment formed from o-dopaquinone polymerization. Melanin is responsible for dark spots on the skin in middle-aged and elderly individuals and of hyper-pigmentation involved in several dermatological disorders such as melasoma, solar lentigines and ephilides.17 Therefore, tyrosinase inhibitors may be clinically used for the treatment of some skin disorders associated with melanin hyperpigmentation and are also important in cosmetics for skin whitening effects. SkinCeraTM was proven to inhibit mushroom tyrosinase in-vitro (50 % inhibition at 100 µg/mL) and to have high affinity with the enzyme in a molecular docking study (Figures 3 and 4A).

Elastase and collagenase are key enzymes involved in the degradation of the extracellular matrix. In particular, they cleave elastin and collagen, responsible for skin elasticity and skin tensile strength respectively.18 Compounds having anti-elastase and anti-collagenase properties may consequently help to prevent age-associated destruction of elastin and collagen, which cause visible signs in aged skin such as wrinkles and sagging. SkinCeraTM was proven to inhibit elastase and collagenase in-vitro and to have affinity to the two proteins in in-silico studies (Figures 3 and 4B and 4C).

Finally, the moisturizing effect of skin was directly estimated by its capacity to retain water and the moisturizing ratio was estimated at 92 % after 8 hours at 35°C and 40 % humidity. (Figure 5)

Figure 2: LC/MS analysis of konjac extract

Figure 3: In vitro anti-tyrosinase, anti-elastase and anti-collagenase activities

14 nutraceuticals now

Bühler’s new data-driven optical sorter, for minimising aflatoxin contamination in maize – with cloud connection for data-analysis.

Figure 5: Moisturizing effect of SkinCeraTM

REFERENCES1 R.O. Potts et al. (1984) “Changes with age in the moisture content of human skin.” The journal of Investigative dermatology, 82, 97-100.2 R. Ghadially et al. (1995) “The aged epidermal permeability barrier.” The journal of clinical investigation, 95, 2281-2290.3 J. Rogers et al. (1996) “Stratum corneum lipids: the effect of ageing and the seasons.” Arch Dermatol Res, 288, 765–770.4 G. Imokawa et al. (1991) “Decreased level of ceramides in stratum corneum of atopic dermatitis: an ethiologic factor in atopic dry skin?” The journal of investigative dermatology, 96(4), 523-526.5 A. Di Nardo et al. (1998) “Ceramide and cholesterol composition of the skin of patients with atopic dermatitis.” Acta Derm Venereol (Stockh), 78, 27–30.6 L. Coderch et al. (2003) “Ceramides and skin function.” Am J Clin Dermatol, 4(2), 107-129. 7 S. Guillou et al. (2010) “The moisturizing effect of a wheat extract food supplement on women’s skin: a randomized, double-blind placebo-controlled trial.” International Journal of Cosmetic Science, 2010, 1–8.8 M. Hori et al. (2010) “Double-Blind Study on Effects of Glucosyl Ceramide in Beet Extract on Skin Elasticityand Fibronectin Production in Human Dermal Fibroblasts.” Anti-Aging Medicine, 7(11), 129-142.9 K. Tsuji et al. (2006) “Dietary glucosylceramide improves skin barrier function in hairless mice.” Journal of Dermatological Science, 44, 101—107.10 R. Ideta et al. (2011) “Orally administrated glucosylceramide improves the skin barrier function by upregulating genes associated with the tight junction and cornified envelope formation.” Biosci Biotechnol Biochem, 75(8), 1516-1523.11 M. Chua et al. (2010) “Traditional uses and potential health benefits of Amorphophallus konjac K. Koch ex N.E.Br.” Journal of Ethnopharmacology, 128, 268–278.12 R. F. Tester and F.H. Al-Ghazzewi. (2015) “Beneficial health characteristics of native and hydrolysed konjac (Amorphophallus konjac) glucomannan.” J Sci Food Agric, Mini review.13 E. Bateni et al. (2013) “The Use of Konjac Glucomannan Hydrolysates (GMH) to Improve the Health of the Skin and Reduce Acne Vulgaris.” American Journal of Dermatology and Venereology, 2(2), 10-14.14 H. Kimata. (2006) “Improvement of atopic dermatitis and reduction of skin allergic responses by oral intake of konjac ceramides.” Pediatric Dermatology, 23(4), 386-389.15 T. Uchiyama et al. (2008) “Oral intake of glucosylceramide improves relatively higher level of transepidermal water loss in mice and health human subjects.” Journal of health science, 54(5), 559-566.16 S. Parvez et al. (2007) “Naturally Occurring Tyrosinase Inhibitors: Mechanism and Applications in Skin Health,Cosmetics and Agriculture Industries.” Phytotherapy Research, 21(9), 805-816.17 T. SA Thring et al. (2009) “Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants.” BMC Complementary and Alternative Medicine, 9, 27, 11 p18 K. Park. (2015) “Role of Micronutrients in Skin Health and Function” Biomol Ther 23(3), 207-217

Figure 4: Molecular docking study: Interaction of SkinCeraTM with Tyrosinase (A), Elastase (B) and Collagenase (C)

16 nutraceuticals now nutraceuticals now 17

Skin sensitivity is usually defined by unpleasant sensations in response to aggressions or factors that normally should not provoke such reactions. Itching, tingling, redness, blushing, irritation, all these symptoms are various manifestations of skin sensitivity. These reactions can be induced by many external stressors (UV exposure, extreme weather conditions, dry heat, indoor or outdoor pollution, smoking, poor food habits and alcohol consumption, inadequate cosmetic habits) but also by internal factors (hormonal change, emotional stress or alteration of the barrier function of the skin)1. Certain subjects have inherent reactive skin that can be due to genetic predisposition or other skin disorders, others have skin sensitized by environmental aggressions, and some just experience temporary sensations and feelings of sensitive skin. As different part of the skin, face, hair scalp or any body parts, like hands, can all be affected by sensitivity. In its origins, its inducing factors and its manifestations, skin reactivity is extremely diverse which makes it an even more complex skin concern.

Several epidemiological studies have estimated the incidence of sensitive skin close to 50%2. Self-reported hypersensitivity in female population is higher than in male population (usually close to twice more) but not always confirmed by objective evaluation like patch tests2. Higher sensitivity of certain skin type is unclear, but it has been suggested that certain ethnic skin groups could react more to certain factors and others groups rather to other factors2.

With an increased awareness to skin health and dermatology globally, the escalation of pollution and the rise of certain inadequate habits and stress, the issue of skin sensitivity is growing and is expected to continue to grow.Consumers in quest of convenient, natural and efficient solutionsConsumers, especially urban populations, are always looking for more efficient and convenient solutions to take care of their skin. Nutritional solutions offer the advantage of a deep, global and long term action in just a simple gesture, like taking one capsule a day. Also they are paying more and more attention to labels and origin of every product they purchase. This can be considered even truer for a person with sensitive skin, as skin reactivity undoubtedly drives awareness toward natural and additive free products.

While in certain case subjects with sensitive or irritated skin cannot bear anymore topical products, nutricosmetics can be seen as an excellent complementary approach to cosmetic products to take care of sensitive skin. In&Out care of reactive skin will combine instant relief, synergistic actions and profound effects.The boom of nutricosmetics market drives the need for differentiating productsThe nutricosmetic market has been on the rise since several years now. Initially an Asian (Japan and Korea) market, it has well developed in Europe and is now booming in North America. It is estimated to reach more than 7 billion dollars in 2020 and continue to expand with a 7 to 10% annual growth.The growth of this market both attracts newcomers and forces existing players to continuously innovate and diversify their offer. We shall expect a further segmentation of the market with products offering new consumer claims, targeting more specific skin types and population or coming in new formats.

SEPIBLISS™, a science driven, nature inspired and consumer relevant ingredientWith a strong expertise in skin biology and a deep knowledge of the cosmetic market, SEPPIC has been a pioneer in nutricosmetics. Anticipating food supplement brands’ need and consumer’s expectations SEPIBLISS™ has been designed as the first oral natural ingredient dedicated to sensitive skin.

SEPIBLISS™ is a virgin coriander seed oil. It is locally produced in the South West of France and its manufacturing process does not involve any solvent. It contains no additives, and is suitable for vegetarian, kosher and halal diets. It has been inspired by nature and tradition, as coriander has been a widely used spice and medicinal plants, from the Egyptians to today trendy cuisine, for its subtle flavor and its active phytochemical components. SEPIBLISS™ has a standardized fatty acid profile with a high concentration (minimum 60%) of the rare petroselinic acid. It also contains linoleic acid, phytosterols and vitamin E.

SEPIBLISS™ has been developed to offer a comprehensive soothing effect for sensitive skin: reducing skin reactivity to skin stressors via a neuro-soothing action and an anti-inflammatory action, and better protecting the skin against skin aggressions, via an antioxidant action and a barrier reinforcing action.

As the first nutricosmetics ingredient specifically targeting skin reactivity, SEPIBLISS™ offers opportunities to develop new food supplement formula dedicated to soothing and reducing symptoms of skin reactivity or more classic nutricosmetics formula (hydration, anti-aging, UV, glowing) that are specific for sensitive skin. Neuro-soothing actionSensory neurons are able to collect information at skin level. They have receptors implicated in the perception of itch and pain, also called nociception. One of them, TRPA1 is activated by environmental irritants like isocyanantes, acrolein from smokes, ozone and carbon oxides, by bacterial endotoxins, by cold temperatures and by reactive oxygen species3,4. TRPA1 is also upregulated under inflammatory conditions5.

The neuro-soothing effect of SEPIBLISS™ was assessed with an in vitro assay on human keratinocytes-neurons co-culture model. Neurons were stimulated and TRPA1 activated with AITC and the protection against this activation by SEPIBLISS™ was evaluated6.

Graph 1 - Activation of TRPA1 following stimulation with AITC

SEPIBLISS™ natural and innovative solution to comfort sensitive skin orallySkin reactivity a complex, diverse and frequent concernSkin being the primary interface between the body and the environment, it provides the first line of defense against physical, chemical and microbial aggressions. Every skin does not react equally to aggressions: some are more sensitive than others. Several terms are used to describes this similar skin condition: sensitive skin, reactive or hyper-reactive skin, intolerant or irritable skin1

SEPIBLISS™ at 0.001% allowed a significant protection of keratinocytes-neurons co-culture from AITC activation by 61%. These results would suggest that SEPIBLISS™ could reduce pain sensation, itching and neurogenic inflammation.Anti-inflammatory actionIn the epidermis, keratinocytes are the first cells encountered by external stimuli and they are able to promote an inflammatory response by increasing the production and the release of various soluble factors, such as cytokines, chemokines (like TNF-α for example) and anti-microbial peptides. Several transcription factors can be activated by such stimuli7. Among them, one of the most studied inflammation-activated transcription factors is Nuclear Factor (NF)-κB. NF-κB monitors the expression of genes involved in inflammatory and oxidative stress response8. This natural reaction to aggressions induces well known symptoms like redness and heat sensations.

The anti-inflammatory effect of SEPIBLISS™ was assessed with in vitro assay on keratinocytes inflammation model9.

Graph 2 - Activation of NF-κB following stimulation with TNF-α

SEPIBLISS™ at 0.0001% allowed a significant protection of keratinocytes from NF-κB activation by 48%. These results would suggest that SEPIBLISS™ could protect keratinocytes from excessive inflammation and prevent associated redness, heat sensation and neuron activation.Protective actionSeveral factors like UV or smoke create reactive oxygen species and induce oxidative chain reactions. These phenomena happens at skin cell level and ultimately they create skin damages and enhance inflammation. Phytosterols, tocopherols and tocotrienols contained in SEPIBLISS™ are potent lipidic antioxidant compounds. They offer a reinforced antioxidant protection against reactive oxygen species induced by UV and chemical aggressors and limitation of inflammation.

The barrier function of the skin can be impaired and make the skin less protected against aggressors. Linoleic acid is the most abundant poly-unsaturated fatty acid in human skin and is a fundamental precursor required for epidermis structure and barrier function. Linoleic acid contained in SEPIBLISS™ can help reinforcing the protection against physical and chemical aggressors.

Conclusion

Figure 1 - Summary of the phenomena involved in skin reactivity and of SEPIBLISS™ soothing action

Being a multifactorial condition and experienced differently according to each subject, skin sensitivity has to be approached through its different mechanisms of action and manifestations. This is why SEPIBLISS™ has been developed as an ingredient with a comprehensive action on skin reactivity.

Authorized for use in food supplements in the EU and in the USA and with an established daily dose of 200 to 400 mg per day, SEPIBLISS™ offers a new and unique approach to take care of sensitive skin by oral route.

References:1 Putative neuronal mechanisms of sensitive skin, Sonja Stander et al, Experimental Dermatology 2009; 18: 417–423.2 The Sensitive Skin Syndrome, Hadar Lev-Tov, Howard I Maibach, Indian J Dermatol. 2012 Nov-Dec; 57(6): 419–423.3 Breathtaking TRP channels: TRPA1 and TRPV1 in airway chemosensation and reflex control, Bessac BF, Jordt SE, Physiology (Bethesda). 2008 Dec;23:360-70.4 TRPA1 contributes to cold hypersensitivity, Del Camino et al, J Neurosci, 2010;30(45):15165-74.5 TRPA1: A gatekeeper for inflammation, Bautista DM et al. Annu Rev Physiol. 2013;75:181-2006 Neuro-soothing effect of SEPIBLISS™, in vitro assay on human keratinocytes-neurons co-culture model, Seppic proprietary data, unpublished7 Role of fibroblasts in the regulation of proinflammatory interleukin IL-1, IL-6 and IL-8 levels induced by keratinocyte-derived IL-1, Boxman IL et al, Arch Dermatol Res. 1996 Jun;288(7):391-8.8 Models for the study of skin wound healing, The role of Nrf2 and NF-κB. Ambrozova N et al, Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2017 Mar;161(1):1-13.9 Anti-inflammatory effect of SEPIBLISS™, in vitro assay on keratinocytes inflammation model, Seppic proprietary data, unpublished

Contact:nutrition.seppic@airliquide.com

By Emilie Bailbled, Marketing Manager

18 nutraceuticals now nutraceuticals now 19

PhytoflORAL® is composed of a proprietary ingredient derived from non-GMO tomatoes rich in the colourless carotenoids, phytoene and phytofluene. It is available as a free-flowing, all-natural powder that can be used in multiple applications, including capsules, chewable tablets, drinks and pre-mix sachets.

“The recent acquisition of IBR by Frutarom opened a new market of cosmeceuticals, with prospects for rapid growth and added value,” says Yoni Glickman, President of Frutarom Natural Solutions. “IBR brings innovation and all-natural solutions backed by research that perfectly fit into our portfolio.”

Clinical data show that dietary intake of phytoene and phytofluene results in the accumulation of these phytochemicals in the skin, delivering multiple skin-health and beauty benefits. These include protection against oxidative damage and sun exposure, as well as capabilities to brighten skin and even out complexion. Moreover, these compounds possess inherent anti-aging properties for skin. These carotenoids inhibit melanin synthesis and control pigmentation through natural mechanisms, assist protecting from UV and oxidative damage, while concurrently reducing inflammation and DNA damage.

In many cultures flawless skin is correlated with luxury and well-being, while even-toned skin is universally translated into a healthier and more youthful appearance.

“Asia Pacific is one of the top markets for beauty from

within ingredients,” explains Liki von Oppen-Bezalel, Ph.D., VP of Business Development and Marketing for IBR. “Multiple skin health ingredients, especially so-called ‘skin-whitening’ materials, are unsafe for use. This is why it was vital to develop a safe, research-backed natural ingredient like PhytoflORAL®.”

PhytoflORAL® has been subject to a number of clinical and in vitro studies that demonstrate its bioactivity. Efficacy was further elucidated through gene expression analysis.

In one clinical study, PhytoflORAL® presented a significant photoprotective effect after 84 days of supplementation, as indicated by a 20% increase in Minimum Erythemal Dose (calculates shortest exposure to UV radiation before reddening of the skin) in 65% of participants. Another study demonstrated a measurable skin lightening effect in

82% of subjects noticeable after 42 days, and increasing after 84 days. In addition, subjects reported enhanced skin radiance, evenness, hydration, and suppleness, and found their skin to be visually healthier and more resistant to sun damage.

IBR developed complementary ingredients to PhytoflORAL® for topical use. These products also are rich in phytoene and phytofluene, and available for dual “beauty from inside and outside” regimes.

The global active ingredients market for cosmetics was valued at approx. US$2.63 billion in 2015. It is projected to reach US$4.45 billion by 2026, as reported in Markets and Markets. The primary drivers for this acceleration is increasing awareness among consumers toward skin health and rising demand of consumers to look good. The trend is especially strong in Asia and South America. This awareness is generating a significant demand for cosmetics and personal care products, particularly those for anti-aging, skin-tone evening, and sunscreen. The demand for natural, “green,” environmentally friendly cosmetic products is likewise increasing.

Frutarom enters “Beauty from Within” market in Asia

Probiotics can boost sports nutritionPure tomato powder-derived ingredient

backed by research boosts skin health and

radiance

Frutarom enters the “beauty from within”

market with PhytoflORAL®, a

patented clinically studied cosmeceutical

that gives skin a light, even tone and promotes

skin health. It was developed by IBR, Ltd., Israel. Frutarom will

showcase PhytoflORAL® at Vitafoods Asia in Singapore, September 11–12, 2018,

at booth #H22.

According to recent US survey [1] conducted on 450 participants, for consumers of vitamin and sports nutrition products, health maintenance and immunity are by far the two first drivers of the purchase decision (Fig. 1). When it comes to choice, ingredients listing and functionality claims are by far the most important for those health-minded consumers who are hungry for information and read about product functionality and efficacy. Sports nutrition is a booming market and while there is a need for well documented ingredients with targeted functionalities, probiotics are well positioned to meet this market.

Sport and immunityWhen gaining in intensity, exercise can be the source of physiological and psychological stress and the body’s natural defences can be impaired. The relationship between the intensity of exercise and immunity is a J-shaped curve (Fig. 2). It appears that people are more prone to bacterial and viral infections following intense training. This phenomenon can last for up to 72 hours [2]. Lowered immunity, combined to hyperventilation that naturally occurs during exercise, contributes to a higher susceptibility to upper respiratory tract (URT) disorders in sportsmen and women. URT infections (URTIs) are a common cause for missing training or even a competition and can affect performance.

The benefits of certain probiotic strains on immune defenses is now well described. One particular strain, Lactobacillus helveticus Lafti® L10 , has been clinically tested in athletes and shows immune support benefits.

A clinical study was published in 2016 that confirmed the benefits of Lactobacillus helveticus Lafti® L10 in training athletes during winter [3]. Based on this and previous clinical studies, the Canadian health authorities (Health Canada) has granted claims such as “Promotes gastrointestinal health in physically active adults” and “Helps reduce the incidence of cold-like symptoms in adults with exercise-induced stress” for this unique probiotic strain. The 39 elite athletes enrolled in this study were aged 18-28 years, training for over 11 hours per week in various sports and followed the probiotic treatment,

or placebo, for 14 weeks in winter period. This randomized, double-blind placebo controlled study looked at the severity, the incidence and the duration of URTIs episodes using validated scale, and monitored biological immune markers.

Figure 3: Effect of the probiotic treatmenton average infections duration (URTI) (Marinkovic et al., 2016).

The study showed that L. helveticus Lafti® L10 supplementation: Significantly shortened by 3.4 days the average duration of URTIs (7.25 days with the probiotic vs. 10.64 with placebo, P<0.05) (Fig. 3).Significantly decreased the number of symptoms by around 29% (4.92 symptoms with the probiotic vs. 6.91 with placebo, P<0.05. URTI severity tended to decrease (P=0.078).Increased the self-rated sense of vigor and tended to reduce the proportion of athletes reporting impaired training.

Moreover, several changes in immune markers have been recorded during the study in the probiotic group: for example, Interferon-γ was significantly higher in the probiotic group suggesting a better prevention of infections

This study reinforces previous findings on the immunological benefits of L. helveticus Lafti® L10 supplementation in athletes it does show that probiotics could help address the increased URT infection risks linked to training and intense exercise and help support a well-functioning immune system. The first sport study conducted with same strain was a pre-post intervention

Figure 2: The relationship between intensity of exercise and infection risk (Nieman, 2008).

Figure 1.

20 nutraceuticals now

COMPANY NEWS . . . COMPANY NEWS . . . COMPANY NEWS-

Probiotic strain B. lactis LAFTI® B94 is supported by clinical evidence for its gut health benefits in children and adolescents. As part of Lallemand Health Solutions’ Hearty Kids and Teens range, B. lactis LAFTI® B94 has been thoroughly characterized and selected for the in vitro and in vivo documentation of its modes of action and its high synergy with prebiotics. The Canadian Health Authorities have recently recognized its beneficial usage for occasional constipation and bloating in children and teens.

NutraIngredients - Asia Awards 2018 aim at rewarding true innovation and cutting edge research in functional foods, supplements and nutrition. More information about the awards and ceremony can be found on: https://www.nutraingredientsasia-awards.com/About Lallemand Health SolutionsBacked by a rich history and 80 years of expertise in probiotic

research and development, Lallemand Health Solutions offers a full line of ready-to-market probiotic formulas and helps its partners to design their own custom & complex formulations using Harmonium, Lafti® or Rosell® Probiotic strains together with our proprietary protective technologies. Because, from the lab to the shelf, Lallemand Health Solutions controls the overall manufacturing process of its products, the company can ensure customers are receiving the highest quality standard of probiotic formulation. Cooperation spells success: we provide our partners with the full support they need to develop, register, and market their products in their own market, making Lallemand Health Solutions a complete probiotic solutions provider.

Lallemand Health Solutions covers more than 60 countries across five continents and is ready to service your probiotic needs. For more information, please visit www.lallemand-health-solutions.com

B. lactis LAFTI® B94 finalist of Probiotic Product of the Year of the Nutraingredients-Asia awards 2018

Meet the team at Vitafoods Asia, Sept. 11 - 12 in Singapore Booth #O36Montreal, Canada... Lallemand Health Solutions is pleased to announce that its documented probiotic strain B. lactis LAFTI® B94 has been shortlisted as finalist for the NutraIngredients - Asia Awards 2018 in the category Probiotic Product of the Year. Final winners will be announced during the awards ceremony on Monday, September 10, 2018 in Singapore, before the start of Vitafoods Asia trade show. Lallemand Health Solutions team will showcase B. lactis LAFTI® B94 and other innovative probiotic strains and solutions on Booth O36 during Vitafoods Asia, Sept. 11 - 12 at Sands Expo & Convention Centre at Marina Bay Sands, Singapore. With over 300 exhibitors and 5,500 visitors expected, this trade show is seen as the leading food and nutrition hub in Asia. We hope to meet you there!

study conducted in two groups of well-trained recreational athletes: 18 ‘healthy’ athletes and 9 fatigued athletes, suffering of fatigue, recurrent sore throats, and impaired performance. In this study, L. helveticus LAFTI® L10 treatment restored the impaired immune response: after one month of probiotic administration, CD4+ T cells secretion of IFN-γ in fatigued athletes was restored (Fig. 4). For the healthy group, IFN-γ level in saliva also increased [4

Figure 4: Effect of a four-week L. helveticus Lafti® L10 treatment on the levels of mediator cells implicated in the immune response (INF-γ) in athletes (Clancy et al., 2006).

Winning: a gut feeling!Gastro-intestinal (GI) functions are also affected during exercise. The body mobilizes its blood supply to the lungs and heart, to the detriment of other organs. This phenomenon can lead to perturbations in gut motility and damages to intestinal epithelial cells increasing gut permeability [5]. Exercise-induced GI disturbances are revealed by diarrhea, vomiting and acid reflux that can impair training and performances.

Probiotics’s ability to help balance and restore the intestinal microbial ecosystems is public knowledge. Their modes of action are increasingly well apprehended. It has been shown that probiotic bacteria can adhere to the gut epithelium, preventing the adhesion of pathogens. Certain strains have the ability to enhance the gut barrier function through various mechanisms. For example, it has been shown that L. helveticus Rosell®-52 has the capability to reinforce the gut barrier by strengthening the tight junctions between epithelial cells, leading to a reduced infection incidence [6], which could be relevant to overcome increased gut permeability. The probiotic yeast S. boulardii also has the ability to communicate with gut lining cells (enterocytes) and help them preserving their integrity and normal metabolism [7]. In addition, this probiotic yeast also shows a positive effect on epithelial cells tight junctions [8].

Gastro-intestinal health and diarrhea prevention are strong areas of clinical evidence for probiotics. For example, the association of Lactobacillus rhamnosus Rosell®-11 and Lactobacillus helveticus Rosell®-52 is the subject of 21 clinical studies in digestive health [9]. Thus, probiotics can appear as valuable strategies to alleviate the impact of exercise on gastro-intestinal functions.

Mens sana in corpore sano “A healthy mind in a healthy body”: if sport is part of a healthy life style , it can also generate stress, for example when overtraining or preparing for a competition. If a certain level of stress can be beneficial to keep oneself competitive and help push the limits, excessive stress can affect exercise. This in turn can impact gastro-intestinal health, as we are now aware that the gut is our second brain, leading to abdominal pain or intestinal discomfort for example.

The role of the digestive microflora and probiotic supplementation on the brain-gut axis has recently gained momentum [10]. In 2008 was published the first clinical trial

linking probiotics to chronic stress symptoms. A double-blind, placebo controlled, randomized study [11] was conducted over a 3-week period in chronic stress sufferers, using either a specific probiotic preparation (Lactobacillus helveticus Rosell®-52 and Bifidobacterium longum Rosell®-175 in a stick format - Probio’Stick®-), or a placebo. After three weeks of daily administration of the probiotic, two important stress-induced gastrointestinal symptoms, nausea and abdominal pain, were significantly reduced by half as compared to the placebo group. This study indicated that the probiotic could bring benefits to chronic stress sufferers, reducing significantly gastro-intestinal symptoms of stress. Later on, another unique clinical study [12] assessed the effect of this probiotic combination on psychological symptoms of stress, using tests commonly used to evaluate anxiolytic drugs. A one-month daily administration of the probiotic had a beneficial effect on general signs of anxiety and depression. These positive results were verified by the decrease of the biological marker for stress (cortisol).

In conclusion, specific probiotic strains can represent novel approach to sports nutrition. Their ability to help restore and maintain microflora balance and natural defences can lead to respiratory tract protection, alleviation of exercise-induced gastro-intestinal disturbance and the effects of stress in active people, from professionals sportsmen to everyday’s life athlete.

References1. Drug Store News; ECRM; Hellawella 2015 (US consumer study conducted on 450 participants)2. Nieman, D. C. (2008) Immunonutrition support for athletes. Nutrition reviews 66(6): 310-3203. Marinkovic D. et al. (2016) Lactobacillus helveticus Lafti® L10 supplementation reduces respiratory infection duration in a cohort of elite athletes: a randomized double-blind placebo-controlled trial. Applied Physiology, Nutrition, and Metabolism, 10.1139/apnm-2015-05414. Clancy et al. (2006) Reversal in fatigued athletes of a defect in interferon γ secretion after administration of Lactobacillus acidophilus. Br J Sports Med 2006; 40:351–3545. Van Wijck, K., et al. (2011) Exercise-induced splanchnic hypoperfusion results in gut dysfunction in healthy men. PLoS One 6(7): e22366.6. P.M. Sherman et al. (2005) Probiotics Reduce Enterohemorrhagic Escherichia coli O157:H7 and Enteropathogenic E. coli O127:H6-Induced Changes in Polarized T84 Epithelial Cell Monolayers by Reducing Bacterial Adhesion and Cytoskeletal Rearrangements. Infection and Immunity, 2005; 73 (8): 5183-5188.7. Kelesidis T. Pothoulakis C. (2012) Efficacy and safety of the probiotic Saccharomyces boulardii for the prevention and therapy of gastrointestinal disorders. Ther Adv Gastroenterol, 2012; 5(2) 111–1258. McFarland, L. V. (2010) Systematic review and meta-analysis of Saccharomyces boulardii in adult patients. World Journal of Gastroenterology: WJG 16(18): 2202.9. Foster L.M., Tompkins T.A. and Dahl W.J. (2011) A comprehensive post-market review of studies on a probiotic product containing Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011. Beneficial Microbes, December 2011; 2(4): 319-33410. Dinan TG, Stanton C, Cryan JF. (2013) Psychobiotics: a novel class of psychotropic. Biol Psychiatry. 2013 Nov 15;74(10):720-6.11. Diop L, Guillou S, Durand H (2008) Probiotic food supplement reduces stress-induced gastrointestinal symptoms in volunteers: A double-blind, placebo-controlled, randomized trial. Nutr Res 28(1) 1-5 12. Messaoudi M. et al. (2010) Assessment of psychotropic-like properties of a probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175) in rats and humans. Br J Nutr 26 1-9

22 nutraceuticals now nutraceuticals now 23

Shifting the focus from carb utilisation to fat burningMost popular sports nutrition drinks, gels and bars on the market today have been designed to increase energy during exercise, yet these products traditionally contain high glycaemic carbohydrates like maltodextrin, glucose syrup and sucrose. Such carbohydrates release glucose into the bloodstream at a fast rate. This is fine for those in need of instant energy for intensive athletic training, such as sprinting. However, for casual fitness users looking to tone their bodies and reduce fat, high glycaemic products can trigger large peaks and troughs in both blood glucose and insulin levels during physical training, minimising not maximising their fat burning potential.

With this in mind, today’s high glycaemic sports nutrition products are not suited to the needs of casual fitness users, or those beginning to exercise. The ideal sports nutrition product for those looking to burn fat and tone whilst exercising needs to be one which contains a low glycaemic carbohydrate and has a lower impact on blood sugar levels.

A low glycaemic carbohydrate such as BENEO’s Palatinose™ (isomaltulose) is ideal for use in sports nutrition products, as it has a balanced effect on blood sugar levels and is fully digestible. It provides full carbohydrate energy in a sustained way, eliminating unwanted “boost and crash” blood sugar spikes and it helps to burn fat more effectively. An increased fat burning rate also means that active consumers can draw on their carbohydrate reserves for longer. These physiological benefits make Palatinose™ ideal for use not only in products aimed at endurance athletes but also for those targeted at casual fitness users or for those who are beginning to get active.

Is there a wider market for sports nutrition products?Research analysts from Globaldata recently commented that next to elite athletes and bodybuilders, there has been an increase in casual fitness users looking to supplement an active, healthy lifestyle with sport nutrition products. At present, despite the forecasted rise in obesity, there are few products targeting obese or overweight consumers. However, Globaldata predicts this will change and we will see more product launches aimed at the overweight, as well as women and older consumersii.

Expanding waistlinesThe prevalence of obesity nearly doubled between 1980 and 2014iii and this figure continues to rise. Governments worldwide are working hard to encourage those who are overweight or obese to get active. However, at present, the majority of sports nutrition products on the market do not

support weight management in this group as they contain high glycaemic ingredients that inhibit fat burning rather than support it.

Ageing populationsAnother group that is highly motivated by products that help them manage their weight is the older consumer. With 11.6 million people aged 65 or over in the UK aloneiv and a quarter of the population of Europe expected to be 60 years or older by 2020v, developing and marketing products for this consumer group makes business sense. Especially considering that many of these older consumers have high purchasing power and are increasingly interested in their health and wellbeing.

Essentially versatileThe functional benefits of a product alone often aren’t enough for it to become a regular feature in consumers’ shopping baskets. Good taste and texture are essential if a product is to become a favourite with purchasers. In fact, figures from a recent study by LightSpeed GMI and Mintel showed that 60% of UK consumers would swap a normal snack for a sports nutrition product, but only if it tasted similar, while nearly 50% said that they would consume sports nutrition food and beverage as part of their every-day diet.

With this in mind, carbohydrate ingredients need to be able to deliver in terms of technical, as well as nutritional and functional benefits. BENEO’s Palatinose™ has a natural, mild sugar-like taste and sweetness and is ideal for use in gels and bars, as well as drinks. As a very low hygroscopic free-flowing powder, it is ideal for powder instant beverages or agglomerates and it significantly reduces the water absorption in blends, minimising the risk of common production issues such as caking and lumping. Since it does not absorb humidity from the environment, convenient handling throughout its shelf-life is guaranteed. It is not easily hydrolysed by acids due to its strong molecular link, ensuring beverages to which it is added are able to retain a stable ratio of solute particles, as in the case of isotonic drinks.

Putting it into practiceSpecialists at the BENEO-Technology Center conduct regular recipe trials that undergo stringent sensorial evaluation, to ensure that BENEO ingredients can deliver in terms of performance, taste and texture. In recent recipe formulation trials, Palatinose™ was used to create a reduced glycaemic response sports drink powder. The end result was a powder that delivers exceptional results in terms of having a sugar-like taste, is dissolvable in cold water and has excellent flowability and dispersability:

Alternative Carb Opens Up New Sports Nutrition Opportunities

With obesity rates set to rise over the next five years and the population ageing, the demand for products that promote fat burning and weight management shows significant growth potential. The BENEO-Technology Center has developed a wide range of new product concepts including drinks, foods and gels to meet these needs. Sports nutrition is moving beyond the realm of just the elite athlete and now, thanks to Palatinose™, there is a functional carbohydrate available to meet the needs of the casual fitness user.

BENEO’s Palatinose™ is a smart release carbohydrate, derived from sugar beet. It is unique because of its molecular structure. As a disaccharide Palatinose™ consists of a glucose and fructose molecule – similar to sucrose, however in contrast to sucrose the linkage of the molecules is much stronger, which means the human body digests it fully, yet more slowly. As a result, the full carbohydrate energy (glucose) is provided in a more steady and sustained way. The blood glucose levels stay balanced without sudden ups and down and this also helps to burn fat more effectively. An increased fat burning rate means that active consumers can draw on their carbohydrate reserves for longer.

The health and fitness trend continues to expand its influence and many more consumers now realise the importance of staying fit and following a balanced diet. This has led to a booming global fitness industry and an increased interest in sports nutrition products globally with the market for sports nutrition growing on an average by a healthy 8.5 per cent between 2012 and 2017i. However, with casual and fitness users on the rise, do today’s mainstream sports products fulfil their specific physiological needs? Klaudia Volmer, product manager for functional carbohydrates at BENEO, discusses the importance of blood sugar management during exercise and highlights the potential of alternative carbohydrates for novel target groups in the sports nutrition market.

Referencesi Source: Innova 2018ii Globaldata 2017iii Source: World Health Organisationiv Source: Age UK ‘Later in Life in the UK’ April 2017 – data from mid-2015 Population Estimates UK Office for National Statistics, 2016v Source: Wikipedia: ‘ageing of Europe’ - European Commission (2014). “Population Aging in Europe: Facts, Implications, and Policies.”. Retrieved 1 May 2017.

Chocolate Almond Drink Recipe

nutraceuticals now 2524 nutraceuticals now

A growing trendConsumer interest in health and wellness is at an all-time high. For the food industry this takes many forms, and while for fat and sugar it is about reducing consumption, when it comes to fibre the opposite is true. More is definitely better.

It is known that most western populations still fall short of the recommended fibre intakes despite fibre being often cited as one of the top five ingredients or benefits looked for by consumers*. In 2015 the UK’s Scientific Advisory Committee on Nutrition (SACN), recommended an increase in the population’s fibre intake to 30g per day for an adult. In Germany 30g has been the recommended limit for a number of years. This level is seen as important for achieving a healthy balanced diet which reduces the risk of developing a range of chronic diseases.

At EU level, resistant starch has been included in the harmonised definition of dietary fibre since 2008. Along with raising the recommended daily intake (RDI) of fibre, SACN also confirmed resistant starch as a key dietary fibre along with other dietary fibres like lignin and oligosachharides. This highlights how there is both a need and an opportunity for our industry to find ways to improve the fibre content in recipe formulations and to help bridge this so-called ‘fibre gap’.

Fibre is a recognisable and consumer-friendly term. Consumers are also aware of its health benefits such as improving digestive health, managing blood sugar levels, lowering cholesterol and supporting weight loss. These are all positive associations and we know that claims such as ‘source of fibre’ and ‘high in fibre’, have an impact on consumer choice. In a study of European consumers questioned stated a ‘high in fibre’ claim was very important when making purchasing decisions, ahead of ‘wholegrain’ and ‘high in protein’ claims*. For a product to secure a ‘source of fibre’ claim, a ratio of three grams of fibre per every 100 grams is required, rising to six grams for a ‘high in fibre’ claim as defined and regulated by Regulation (EC) 1924/2006 on nutrition and health claims. Being able to draw on wider forms of fibre, such as resistant starches, means that these types of claims are more readily achievable across a variety of applications.A recipe for successThis begs the question, are there particular applications and food categories that are more suited to a fibre claim, both in terms of consumer expectation and also processing capability? For consumers, there is perceived to be a natural affinity between baked goods such as bread, cereals and pasta, where a large proportion of the recipe might be flour, wheat, rye or oat-based, and a fibre claim. These low moisture applications require a fibre ingredient that can offer a low water-holding capacity to ensure the processability is maintained. This is where resistant starch – a form of starch that is not digested in the small intestine – can play a role. With a low water holding capacity, resistant starch enables manufacturers to use naturally occurring.

There are also bakery products that have until now proven more difficult to improve the fibre content of, such as white breads, cereals, cakes and muffins, due to consumer expectation and preferences as well as the availability of ingredients. Resistant starches, however, such as Ingredion’s HI-MAIZE® 260, are an ‘invisible’ type of high fibre. They can simply be used to directly replace a significant part of the flour, making it easier to use than other types of fibre.

The very small particle size of the starch, as well as

its neutral flavour and white colour, make it possible to add hidden fibre goodness without affecting the quality and appearance of a baked good. This is an excellent way to enhance the nutritional content of indulgent but nutritionally-low baked goods, such as cookies and savoury snacks, and in wider applications areas including products for children, without impacting overall consumer appeal. It is also relevant to the growing gluten-free sector where historically products were perceived to be of poor nutritional value and difficult to process. As, for example, HI-MAIZE® 260 resistant starch is naturally gluten-free and easy to incorporate, it is a good way to produce high quality, nutritionally improved gluten-free baked goods. It is also a recognisable ingredient, listed simply as ‘starch’ on the label, and therefore supports a clean label approach.

In addition to raising the suggested fibre intake for adults, for the first time the UK’s 2015 SACN report introduced guidelines for those under 16 years in an attempt to increase intakes of fibre among children. This is another example of where ‘invisible’ fibre can have a positive effect. For example, formulating a white bread using HI-MAIZE® 260, which has a resistant starch content of 60%, significantly improves its nutritional value and provides a ‘high fibre’ claim while maintaining the same appearance, taste and quality of bread and improving shelf-life stability. Similarly, breakfast cereals and nutrition bars can benefit from a resistant starch that offers a similar water holding capacity to wheat flour, making it suitable for low moisture applications.Making a claim Raising the fibre content of food products by incorporating resistant starches can also allow food manufacturers to apply a health claim as well as a fibre-related one. Consumers respond well to front-of-pack claims such as ‘helps maintain healthy blood sugar levels’ and ‘reduced glycaemic response’, especially given the rising incidence of type-II diabetes and the lifestyle trend towards following fuller for longer diets. A recent comprehensive review by the British Nutrition Foundation (BNF), ‘Health effects of resistant starch’, also revealed that resistant starch possesses positive properties as a healthy food component which is encouraging. There is also emerging science indicating that resistant starch may also help with insulin resistance, a precursor of diabetes and thus may help to reduce the risk of developing diabetes mellitus.

Ingredients such as resistant starch that are able to combine an approved health claim with ease of processing are an effective way to formulate with fibre and differentiate products. However, this is a highly regulated area and only a limited number of fibre ingredients can support a health claim. These claims must be backed up by scientific evidence to gain EU approval. For example, Ingredion’s HI-MAIZE® 260 has an approved EU health claim, substantiated by EFSA, that states it contributes to a reduction in blood glucose rise after a meal if the final content of resistant starch is at least 14% of the total starch.

To dramatically improve the fibre content of our diets we

BRIDGING THE FIBRE GAP Mona Schmitz-Hübsch, European Senior Marketing Manager Wholesome, Sweetness and Nutrition at Ingredion EMEA, looks at how the food industry can increase the fibre content of foods to improve the population’s fibre consumption and meet the growing consumer demand for products with high fibre claims.

also need to look at how fibre can be used in applications beyond the traditional areas of bread and cakes. Again, this is where resistant starches can be of benefit. Some are process tolerant and stable, even in more demanding high moisture applications such as nutrient dense medical nutrition products, making them an attractive and versatile ingredient. While many starches and carbohydrates have their fibre properties reduced on cooking, retrograded resistant starches such as NOVELOSE® 330 starch, are temperature resistant and retain their high percentage of dietary fibre content, opening up new opportunities to boost the fibre content in wider food categories including yoghurt, beverages or dairy desserts.

Recent recommendations have shown there is a general need to increase the levels of dietary fibre consumed as part

of a healthy diet across the population. Alongside this, the decision by the UK’s 2015 SACN report to include resistant starch within the dietary fibre calculations is a positive, opening up many new opportunities for the food industry to develop recipes using ingredients that are high in fibre, nutritious and easy to formulate.

*Nielsen Global Health and Wellbeing Survey, Q3, 2014

Referencei Regulation (EC) No. 432/2012 of 16 May 2012, establishing a list of permitted health claims made on foods, other than those referring to the reduction of a disease risk and to children’s development an health. OJEU. L 136/1.

26 nutraceuticals now

The World Health Organization recommends exclusive breastfeeding for the first 6 months of a newborn’s life, with continued breastfeeding along with appropriate complementary foods to the age of 2 years or beyond. Breastfeeding for longer periods has many benefits for the infant, including lower infection rates, and a lower risk of developing asthma, allergies, inflammatory diseases and even obesity. Human breast milk contains all the essential nutrients required to promote healthy infant development but is also a rich source of bioactive compounds such as immunoglobulins, lactoferrin, lysozymes, cytokines and complex carbohydrates. The latter, known as human milk oligosaccharides (HMOs), comprise more than 150 different sugar molecules whose effects on infants` development have been the subject of intense research over the last decade. Breast-fed infants consume 15 grams of HMOs per day on average. The concentration and composition of HMOs in human breast milk varies during the period of lactation and is adapted to the needs of the child at each stage of development. The simplest HMOs are trisaccharides (three sugar residues) formed by linking the disaccharide lactose to fucose, sialic acid or N-acetylglucosamine. More complex HMOs are based on tetraose core structures (four sugar residues), in which lactose is extended with units of lacto-N-biose (yielding lacto-N-tetraose) or N-acetyllactosamine (yielding lacto-N-neotetraose). Both of these structures can be decorated with fucose and sialic acid or extended by adding more galactose or N-acetylglucosamine residues to make even more complex HMOs.

One of the key benefits of HMOs during human post-natal development is their promotion of a healthy gut microbiome. This community of microbes – mainly bacteria – is so large that it exceeds the number of human body cells (which number in the trillions). Microbes are found throughout the human intestinal tract but are particularly concentrated in the colon. A well-balanced microbiome contains beneficial bacteria that have a positive effect on health, for example by providing enzymes for food digestion or the synthesis of vitamins, whereas a poor microbiome can become overrun with pathogens that cause severe diseases like infectious diarrhea or tissue inflammation. The gut microbiome begins to form before birth when the fetus swallows amniotic fluid containing bacteria to some extent [1]. However, this preliminary microbiome is vastly restructured during birth and in the subsequent days, when the neonate is first exposed to bacteria in the birth canal and then to additional microbes in the environment.

After birth, diet is one of the most important factors that affect the composition of the gut microbiome. Breast milk promotes the growth of so-called pioneer bacteria, such as Bacteroides fragilis, Bifidobacterium infantis and Lactobacillus acidophilus, whereas formula-fed infants mostly lack these bacteria [2]. The explanation for this phenomenon is that HMOs, which are not present in typical formulas, are used by these pioneer bacteria as a source of energy. For example, B. infantis and other bifidobacteria are equipped with specific proteins and enzymes allowing the uptake and metabolization of structurally different HMOs, particularly the fucosylated HMOs 2′-fucosyllactose, 3-fucosyllactose and di-fucosylactose [3][4][5]. The most abundant HMO in the breastmilk of 80% of lactating mothers is 2′-fucosyllactose, whereas the other 20% are known as non-secretors because they lack the enzyme α1,2-fucosyltransferase which is required for the synthesis of 2′-fucosyllactose and other HMOs containing an α1,2-epitope, such as lacto-N-fucopentaose I. Accordingly, bifidobacteria become established more frequently and earlier in the breast-fed infants of secretor mothers compared to those of non-secretor mothers [6].

The abundance of bifidobacteria also correlates with higher concentrations of organic acids and therefor a lower pH [6] [7]. One reason for this is that bifidobacteria can metabolize fucosylated HMOs to produce short-chain fatty acids (SCFAs) such as acetate and also lactate, which increase the acidity of the environment. Some strains of bifidobacteria can also metabolize 3′-sialyllactose and 6′ sialyllactose [5] [8]. Other species in the microbiome (e.g. Lactobacillus casei and L. acidophilus) can utilize more complex HMOs, including lacto-N-triose, lacto-N-tetraose and lacto-N-neotetraose [8] [9]. Given the preference of different bacteria for HMOs with different structures, it is clear that a mixture of

structurally diverse HMOs (neutral undecorated, fucosylated and sialylated HMOs) favor the development of a balanced microbial community. Current infant formula are supplemented with galacto-oligosaccharides (GOS) and fructo-oligosaccharides (FOS) to mimic the prebiotic effect of HMOs, but these molecules are not found in breast milk and their consumption promotes the development of a microbial community that is completely different to that found in breast-fed infants [10].

A well-balanced microbiome early in life has a positive effect on the developing immune system and therefore comes with life-long benefits by reducing the likelihood of asthma and allergies, metabolic diseases such as obesity, inflammatory diseases such as irritable bowel disease and autoimmune diseases such as type 1 diabetes later in life [10][11]. In contrast, microbial perturbations during early infancy can generate a pro-inflammatory environment which increases the likelihood of the diseases listed above. The mechanisms by which the microbiome promotes good health are only now being unraveled, and several different pathways appear to be involved. One pathway involves the production of protective metabolites, particularly SCFAs. These have local and systemic effects. Locally, they increase the pH of the gut and generate an environment that is hostile to many pathogens [12]. But SCFAs also have a systemic anti inflammatory effect that maintains homeostasis in the immune system by regulating T-cell differentiation. Butyrate in particular has a further role in the maintenance of the epithelial barrier in the gut, because it is consumed by colonocytes and regulates tight junction proteins to inhibit inflammation. SCFAs also regulate lipid metabolism and liver glucose homeostasis either directly or by influencing the production of gut hormones. The production of SCFAs during early development trains the immature immune system to tolerate the beneficial bacteria in the microbiome [13]. Another protective mechanism associated with HMO utilizing gut bacteria is the direct modulation of virulence gene expression in pathogenic bacteria thus rendering them harmless. For example, bifidobacteria that utilize 3′-sialyllactose secrete metabolites that inhibit the expression of virulence genes in pathogenic Escherichia coli O157:H7 and Salmonella typhimurium [14].

HMOs not only promote health by favoring the growth of beneficial bacteria in the gut, they also interact directly with pathogens to prevent colonization. HMOs resemble some cell-surface glycan structures that serve as receptors or co-receptors for bacterial or viral pathogens. Therefore, they act as decoys, trapping the pathogens in the lumen and preventing them from interacting with their target cells. Many different pathogens are neutralized in this manner. For example, norovirus and rotavirus are the leading causes of death worldwide in children less than 5 years old, but breastfeeding protects infants against gastroenteritis caused by these viruses. In particular, fucosylated HMOs such as 2′-fucosyllactose and 3-fucosyllactose bind to norovirus particles in the same manner as the virus receptors [15]. Similarly, 3′-siallylactose and 6′-siallylactose were shown to reduce the infectivity of human rotavirus in monkey kidney epithelial cells by directly blocking the uptake of the virus [16]. Indeed, 2′-fucosyllactose, 3-fucosyllactose and 6′-sialyllactose can inhibit the binding of Campylobacter jejuni, enteropathogenic Escherichia coli, Vibrio cholerae, Salmonella enterica serovar fyris and Pseudomonas aeruginosa to intestinal human cells [17][18][19]. As well as blocking infections by acting as decoys, some HMOs have a direct bacteriostatic effect on pathogens such as B type Streptococcus algalactiae and can bind the toxins produced by Clostridium difficile [20] (Table 1).

HMOs not only act as nutrients for beneficial bacteria and decoys for pathogens in the gut, they are also partly adsorbed into the systemic circulation by crossing the epithelial barrier. In this context, they can have direct effects throughout the human body by binding to several classes of lectins (glycan-binding proteins) expressed on dendritic cells and other immune system cells. By binding to these cell-surface structures, HMOs influence the regulation of immune responses to pathogens and the cell-cell communication essential to control inflammation [21]. For example, 2′-fucosyllactose was shown to inhibit inflammatory responses caused by the lipopolysaccharides of Gram-negative bacteria, and specific HMOs abundant in human

Human milk oligosaccharides, not only for breast-fed babies

By Dr. Katja Parschat, Deputy Head of R&D at Jennewein Biotechnologie GmbH

28 nutraceuticals now nutraceuticals now 29

colostrum attenuate mucosal inflammation reactions in the neonate and support the maturation of the intestinal mucosal immune system in newborns [22][23]. One recent clinical study revealed that the cytokine profile of infants fed exclusively on formula containing 2ʹ-fucosyllactose and GOS was identical to that of breastfed infants, but differed significantly from that of infants fed exclusively on formula containing only GOS [24]. A very recent study has shown that dietary 2′-fucosyllactose directly improves vaccine responsiveness in a murine cell-based influenza vaccination in vitro model [25].Not only for breast fed babiesHMOs are known to promote the establishment of a well-balanced gut microbiome, to protect infants from infectious diseases, to directly and indirectly support the maturation of the immune system, and to provide a systemic anti-inflammatory effect. The sialylated HMOs also promote brain development by releasing sialic acid as building block for gangliosides [26] required for neuronal maturation. Thus far, all these benefits have been accessible only for infants fed on human breast milk. In 2016, however, the first infant formula containing the most abundant HMO (2′-fucosyllactose) was launched on the market, and in 2017 a formula containing 2′-fucosallactose and lacto-N-neotetraose became available. The inclusion of structurally diverse HMOs in infant formula elicits broad beneficial effects for the child. Jennewein Biotechnologie GmbH, the pioneer in the industrial production of HMOs, is currently designing ingredient mixtures comprising multiple HMOs as the next innovation step. Accordingly, a clinical trial is underway involving infants less than 2 weeks old fed exclusively with a formula containing the five most abundant HMOs (including fucosylated, sialylated and undecorated oligosaccharides) in their natural concentrations. This mixture of HMOs will bring infant formula one great step forward towards a future when formula provides all the benefits of natural breast milk.References[1] Chong, C. Y. L. et al.: Factors affecting gastrointestinal microbiome development in neonates. Nutrients. 2018. 10(3): 274.[2] Walker, W. A. et al.: The importance of appropriate initial bacterial colonization of the intestine in newborn, child, and adult health. Pediatric research. 2017. 82(3): 387.[3] Garrido, D. et al. A novel gene cluster allows preferential utilization of fucosylated milk oligosaccharides in Bifidobacterium longum subsp. longum SC596. Scientific reports. 2016. 6. Jg., S. 35045.[4] Sela, D. A. et al.: The genome sequence of Bifidobacterium longum subsp. infantis reveals adaptations for milk utilization within the infant microbiome. Proceedings of the National Academy of Sciences. 2008. 105(48): 18964-18969.[5] Bunesova, V. et al.: Fucosyllactose and L-fucose utilization of infant Bifidobacterium longum and Bifidobacterium kashiwanohense. BMC microbiology. 2016. 16(1): 248.[6] Lewis, Z. T. et al.: Maternal fucosyltransferase 2 status affects the gut bifidobacterial communities of breastfed infants. Microbiome. 2015. 3(1): 13.[7] Matsuki, T. et al.: A key genetic factor for fucosyllactose utilization affects infant gut microbiota development. Nature communications. 2016. 7: 11939.[8] Thongaram, T. et al.: Human milk oligosaccharide consumption by probiotic and human-associated bifidobacteria and lactobacilli. Journal of dairy science. 2017. 100(10): 7825-7833.[9] Bidart, G. et al.: The extracellular wall-bound beta-N-acetylglucosaminidase from Lactobacillus casei is involved in the metabolism of the human milk oligosaccharide lacto-N-triose. Applied and environmental microbiology. 2015: AEM-02888.[10] Milani, C. et al.: The first microbial colonizers of the human gut: composition, activities, and health implications of the infant gut microbiota. Microbiology and molecular biology reviews. 2017. 81(4). 2017: e00036-17.[11] Zhou, X. et al.: Early-Life Food Nutrition, Microbiota Maturation and Immune Development Shape Life-Long Health. Critical reviews in food science and nutrition. 2018. just-accepted: 1-37.[12] Sun, Y. and M. X. D. O’Riordan. Regulation of bacterial pathogenesis by intestinal short-chain fatty acids. Advances in applied microbiology. 2013. 85: 93-118. [13] Morrison, D. and T. Preston. Formation of short chain fatty acids by the gut microbiota and their impact on human metabolism. Gut microbes. 2016. 7(3): 189-200.[14] Bondue, P. et al.: Cell-free spent media obtained from

Bifidobacterium bifidum and Bifidobacterium crudilactis grown in media supplemented with 3′-sialyllactose modulate virulence gene expression in Escherichia coli O157: H7 and Salmonella typhimurium. Frontiers in microbiology. 2016. 7: 1460. [15] Weichert, S. et al.: Structural basis for norovirus inhibition by human milk oligosaccharides. Journal of virology. 2016: JVI-03223.[16] Laucirica, D, R. et al.: Milk oligosaccharides inhibit human rotavirus infectivity in MA104 cells. The Journal of nutrition. 2017. 147(9): 1709-1714.[17] Coppa, G. V. et al.: Human milk oligosaccharides inhibit the adhesion to Caco-2 cells of diarrheal pathogens: Escherichia coli, Vibrio cholerae, and Salmonella fyris. Pediatric Research. 2006. 59(3): 377.[18] Weichert, S. et al.: Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines. Nutrition research. 2013. 33(10): 831-838.[19] Facinelli, B. et al.: Breast milk oligosaccharides: effects of 2′-fucosyllactose and 6′-sialyllactose on the adhesion of Escherichia coli and Salmonella fyris to Caco-2 cells. The Journal of Maternal-Fetal & Neonatal Medicine. 2018: 1-3.[20] Craft, K. M. and S. D. Townsend. The Human Milk Glycome as a Defense Against Infectious Diseases: Rationale, Challenges, and Opportunities. ACS infectious diseases. 2017. 4(2): 77-83.[21] Triantis, V. et al.: Immunological effects of human milk oligosaccharides. Frontiers in pediatrics. 2018. 6: 190.[22] He, Y., et al.: Human colostrum oligosaccharides modulate major immunologic pathways of immature human intestine. Mucosal immunology. 2014. 7(6): 1326.[23] He, Y. et al.: The human milk oligosaccharide 2′-fucosyllactose modulates CD14 expression in human enterocytes, thereby attenuating LPS-induced inflammation. Gut. 2014: gutjnl-2014.[24] Goehring, K. C. et al. Similar to those who are breastfed, infants fed a formula containing 2′-fucosyllactose have lower inflammatory cytokines in a randomized controlled trial–4. The Journal of Nutrition. 2016. 146(12): 2559-2566.[25] Xiao. L. et al.: Human Milk Oligosaccharide 2′-Fucosyllactose improves innate and adaptive immunity in an influenza-specific Murine Vaccination Model. Frontiers in immunology. 2018. 9: 452.[26] Wang. B.: Molecular Mechanism Underlying Sialic Acid as an Essential Nutrient for Brain Development and Cognition–3. Advances in Nutrition 2012. 3(3): 465S-472S.

Table 1. Bacterial and viral pathogens inhibited by human milk oligosaccharides (HMOs) [20].

Nutritional proteinsIngredia’s PRODIET® range, with native whey, native micellar casein, milk protein isolate and whey protein hydrolysates allow us to offer to our customers, proteins that meet their needs, with good nutritional and functional benefits. In nutrition, micellar casein is playing an increasingly important role with its scientifically proven nutritional benefits. An optimal amino acid profile and very good chemical score are two inherent properties much sought after in nutrition and that micellar casein offers. Micellar casein is a slow-digested protein with an extended release of amino acids in the blood flow that can last up to seven hours after consumption. The gradual release of amino acids in the blood flow allows the body to boost muscle synthesis. In sports nutrition, this unique digestion profile allows the consumer to experience optimal muscle recovery after physical exercise. In clinical nutrition, proteins contribute to the maintenance of muscle mass, a very important way to support recovery after an hospitalization (for illness or surgery) or to fight sarcopenia that affects elderly people.

- PRODIET® Fluid is a native milk protein isolate (MPI) in spray-dried powder, naturally rich in native micellar caseins, as high as 92%. This unique milk protein contains more than 87% protein on dry matter.PRODIET® Fluid has been developed to meet the needs of the nutrition market. PRODIET® Fluid comes from a non-denaturing membrane filtration process. . PRODIET® Fluid enables the development of high protein ready-to-drink beverage while maintaining fluidity. After heat treatment (pasteurization, UHT), PRODIET® Fluid has a lower viscosity compared to a standard milk protein isolate. . Using PRODIET® Fluid, Ingredia’s team achieved a 14% protein ready-to-drink beverage, while maintaining a good taste (milky taste with bitter notes), and the perfect fluid creamy texture.- PRODIET® Hydrolysate S 25 is a whey protein hydrolysate

with a hydrolysis degree of 25%. Milk Protein Hydrolysates optimize amino acids bioavailability and are suitable for muscle growth. PRODIET® Hydrolysate S 25 will trigger muscle synthesis in the sportsmen or the elderly, thanks to the high content of leucine and its fast assimilation. Dairy bioactivesIngredia, as a key player in dairy bioactives, keeps innovating to contribute to improve people well-being and health. Relying on its know-how and expertise, Ingredia proposes particularly two key bioactives, Lactium® and OsteumTM CPP.₋ Lactium® is a unique, proprietary milk protein hydrolysate, which contains a bioactive peptide with relaxing properties. It has been clinically proven, through 8 clinical studies, to help reducing stress-related symptoms. Throughout those studies, made in accordance with OECD guidelines and Good Laboratory Practices, Lactium® has shown no undesirable side-effects, addiction or toxicity.Lactium® is a natural ingredient already used worldwide by numerous consumers searching an ally for their daily life. It helps to:• Regulate chronic stress symptoms (weight gain/ loss, sleep disorders…), • Face very stressful periods of life (exams, tobacco withdrawal…)• Optimize general well-being.

Osteum CPP , Casein phosphopeptides (CPP), is a bioactive milk protein hydrolysate, very rich in CPP with a ratio over 25% or 35%. It is produced from our native micellar casein coming from 100% cow’s milk of our French dairy cooperative. Osteum CPP binds calcium and keeps it soluble and bioavailable during digestion. Using CPP supplementation allows to reduce bone mass destruction. It meets particularly children, pregnant, postmenopausal women and senior needs.

Ingredia is a French dairy company that develops and manufactures dairy powders, milk proteins and innovative bioactive ingredients for the food, nutrition and health industries worldwide. Ingredia supports its dynamic growth by continued investments in cutting-edge industrial facilities and scientific research and innovation.

30 nutraceuticals now nutraceuticals now 31

Male sexual performance is mainly evaluated by erectile function.

Sexual function is physiologically correlated with age and also with one’s psychological and emotional state. Approximately 50% of men between 40 and 70 years old could be concerned by a decrease of sexual performance (NIH Consensus Conference, 2012, 2013).

A decrease of sexual performance could lead to important psychological consequences such as: anxiety, mood and lack of self-esteem, contributing to a vicious circle.

EnoSTIMTM is a proprietary synergistic formulation of active ingredients – Nexira Proprietary Formulation (NPF) – enriched with saffron. It is a natural ingredient for food supplements.

The Nitric Oxide (NO) pathway is the cornerstone of the blood flow mechanism allowing penile erection. The NO pathway is the most targeted site to improve male performance.

EnoSTIM™ is characterized by a synergistic effect of its

components (Saffron + NPF) at 1 mg/l.:

New study results(quantity equivalent in 1 mg/l of EnoSTIMTM)(1 mg/l)

Nexira has recently unveiled impressive new study results.

“We conducted a study on 94 men, aged 45+, and EnoSTIMTM has demonstrated really good results”, says Damien Guillemet, Nexira Scientific Director.

Two standardized tests were used: the Erection Hardness Score (EHS) and the International Index of Erectile Function (IIEF-5). The study was conducted on men with a sexual desire with a hardness score between 2 and 3 at the beginning of the study.

Consumers have to take 300 mg EnoSTIMTM twice a day during 4 weeks. The study shows impressive results with both EHS and IIEF-5!

With the EHS, significant results are observed after one week. On week 4, 74% of volunteers improve their score. And 1 volunteer out of 2 reaches score 4 at the end of the study (+50% vs the beginning of the study).

With the IIEF-5, after 4 weeks, 72% of consumers have a better IIEF-5 score. 69% volunteers obtain more than 17 points at the end of the study.

Nexira also ran a satisfaction survey at the end of the study, showing very interesting results: 71% consumers would recommend EnoSTIMTM.66% consumers are largely satisfied by EnoSTIMTM and notice better erections.58% consumers are more confident to engage an intercourse with EnoSTIMTM.

EnoSTIMTM, the natural male enhancer Sexual activity is an important indicator of the overall health and well-being of men. A healthy and satisfying sex life is closely associated with a healthy lifestyle.

Mechanism of action: stimulation of nitric oxide productioneNOs (endothelial Nitric Oxide synthase) synthetizes NO which is directly implicated in the blood flow. Studies1, 2 showed that Nexira’s proprietary formulation activates eNOs, which can increase blood flow up to 50%.

Penile erection is caused by an increased blood flow to the penis resulting from the relaxation of the cavernous arteries and corpus cavernosum smooth muscle cells.

Saffron benefitsHistorically, saffron (flower stigmas from Crocus sativus) has been used in traditional medicine. It was already consumed as an aphrodisiac by ancient Persians and Egyptians. More recently, saffron’s ability to improve male performance was demonstrated in several clinical studies3. In Europe, saffron benefits from the following pending claim2: “Improves erection and relaxes muscles”.

ID 2427 on Styli cum Stigmatis Crocus sativus: 28 mg/day

Nitric oxide protectionNitric oxide has a short lifespan and is easily degraded by superoxide free radical.

As shown in an in vivo study4, the antioxidant molecules in Nexira’s proprietary formulation demonstrated the potential to specifically protect NO by decreasing oxidative stress by 74%.

Manufactured in France in our own facility, with our specific know-how, expertise and formulation, EnoSTIMTM is the male enhancer made with natural and plant-based ingredients with a proven efficacy.

Scientific references:1 Effect on Nexira’s proprietary formulation (NPF) on the activation of eNOs through the phosphorylation of “Serine 1177” from endothelial cells (HUVECs) as measured by flow cytometry, 2008.2 NPF stimulates endothelial-dependent blood flow by nitric oxide synthetis. Ex vivo study on rat aorta, 2004.3 Cai T. et al., An improvement in sexual function is related to better quality of life, regardless of urinary function improvement: results from the IDIProst® Gold Study. Arch Ital Urol Androl. 2013 Dec 31;85(4):184-9.4 The effectsof NPF, a formulation containing grape and apple extracts and its effect on the production of peroxinitrites in hamsters subjected to aerobic physical activity. 2004.

COMPANY NEWS . . . COMPANY NEWS . . . COMPANY NEWS-

32 nutraceuticals now nutraceuticals now 33

“Fruit d’Or whole fruit cranberry powder maintains its natural bright red color over three years with little PAC degradation. No other whole fruit cranberry supplier can say this,” states Stephen Lukawski, CEO of RSSI and lead sales consultant and partner for Fruit D’Or Nutraceuticals.

This news is especially significant because Fruit d’Or is the only whole fruit cranberry powder supplier to link its 7% PAC analysis with anti-adhesion activity, through testing by Rutgers University. “Fruit d’Or has worked diligently to establish a higher standard of quality for whole fruit cranberry powders. Fruit d’Or is using advanced analytical equipment such as Maldi-TOF for identification and authentication, polyphenol fingerprinting, updated reference standards and quantitative testing methods to support its outcome studies,” Lukawski explains. “Through identification, standardization and authentication of your starting cranberry powder, clinical studies can now be repeated and validated. We want companies to understand that Fruit d’Or’s vertically integrated farm-to-table operation delivers consistency and provides quality control from lot to lot.

“We’ve shown that daily dosages of 1 gram of our cranberry powder with a minimum of 7% PACs can help prevent colonization of pathogenic E. coli bacteria. We use the DMAC test method to measure soluble PACs and the butanol test method to measure insoluble PACs, this is why it’s critically important to protect the integrity of the whole fruit cranberry. As a supplier, we do our best to prevent PAC degradation by using

state-of-the-art milling techniques and low drying temperatures.”

Lukawski cautioned, however, that if manufacturers use excess heat in their tablet or encapsulation process, or don’t store the ingredients properly, it could affect the PACs’ integrity and efficacy. “In addition, adding lubricating agents or other excipients in the manufacturing process could also likely degrade both the soluble and insoluble PACs, and turn the color of the cranberry from red to brown.” He added, “If companies don’t want to show PAC content on their labels, at least indicate the source of the cranberry and conduct real-time anti-adhesion testing to demonstrate the efficacy of your finished product.” It’s about earning the trust of consumers through transparency and by delivering on promise safe and efficacious cranberry products.

To work with Fruit d’Or Nutraceuticals on delivering best-in-class quality cranberry products to consumers, visit www.qualitycranberry.com .

Fruit d’Or Nutraceuticals first to demonstrate a three-year stability for its PACs in whole fruit cranberry powder

This category leader also is the only company guaranteeing a 7% PAC content

Fruit d’Or Nutraceuticals has been the first cranberry company to identify insoluble PACs in its whole fruit cranberry products. It is now the first cranberry supplier to conduct real-time shelf life testing on the stability of its whole fruit cranberry powder’s soluble and insoluble PACs. The company has confirmed three-year stability of its total PACs and guaranteed a minimum of 7% PAC content in both its brands: Cran d’Or and Cran Naturelle organic

For us, as processors of animal by-products for the production of collagen proteins like gelatins, collagen peptides and collagen, the topic of animal welfare is particularly relevant. Even though the public discussion is primarily focused on the production of meat, due consideration must also be given to animal by-products. Ethical and fair livestock farming is a fundamental building block for the general acceptance of animal products. We firmly believe that the Animal Welfare Initiative is taking the right approach by consciously tackling the topic across the supply chain with large-scale involvement from the industry,” says Reinhard Zehetner, Global VP of Quality and Regulatory Affairs at GELITA AG.

“With GELITA AG, we’ve gained a partner that we can really be pleased with. That’s because GELITA AG proves that the topic of animal welfare is a relevant issue for more and more companies,” says Dr. Alexander Hinrichs, Managing Director of the Animal Welfare Initiative. “GELITA AG is making a statement with its sponsoring membership: the time has come to take responsibility at all stages of the meat supply chain, and the Animal Welfare Initiative offers the possibility of doing this.”

As sponsoring members, companies contribute to creating a more ethical and sustainable meat industry. Companies that are interested in becoming sponsoring members can contact the Animal Welfare Initiative’s office in Bonn.

About GELITA AGGELITA is one of the world’s leading manufacturers of collagen proteins. With 21 production facilities, it is represented on every continent. Collagen proteins are used as gelatin in the manufacture of food, pharmaceutical products and in technical applications. Collagen peptides are active components in the manufacture of products that counteract joint and skeletal problems, build muscle, reduce weight and reduce wrinkles.

With a workforce of over 2,500 employees, the company achieved revenues of €709 million in 2017. The GELITA

Group’s management team is based in Eberbach, Germany. GELITA is an active supporting member of the Rhein-Neckar metropolitan region and is ranked in the Top 100 Innovative Companies.

About the Animal Welfare InitiativeThe Animal Welfare Initiative enables leaders in the

agriculture, meat, and food retail industries across the supply chain for pork and poultry to live up to their collective responsibility for the proper treatment of animals, as well as animal health and safety in the livestock industry. The Animal Welfare Initiative offers financial support to farmers to implement livestock welfare measures that go above and beyond the legal standards. The implementation of these measures is comprehensively monitored by the Animal Welfare Initiative. Founded in 2015, the Animal Welfare Initiative started its second, equally successful three-year program phase in 2018. The Animal Welfare Initiative is establishing greater animal welfare on a broader level, step by step, and continues to grow.

The Initiative’s shareholders are: • Bundesverband der Deutschen Fleischwarenindustrie e.V. [The Federal Association of the German Meat Product Industry]• Deutscher Bauernverband e.V. [German Farmer’s Association]• Deutscher Raiffeisenverband e.V. [German Raiffeisen Association]• Handelsvereinigung für Marktwirtschaft e.V. [Trade Union for Market Economics]• Verband der Fleischwirtschaft e.V. [Association of the Meat Industry]• Zentralverband der Deutschen Geflügelwirtschaft e.V. [Central Association of the German Poultry Industry]

Bonn, Germany, July 11, 2018 – GELITA AG announces it is joining the Animal Welfare Initiative as a sponsoring member. As of August 1, 2018, the company will support the Animal Welfare Initiative with a financial contribution. By becoming sponsoring members, companies that do not directly sell meat and meat products can help improve animal welfare in livestock farming.

GELITA AG joins the Animal Welfare Initiative as a sponsoring member

34 nutraceuticals now nutraceuticals now 35

The global sports nutrition market is estimated at $14.6 billion and is expected to surpass $21 billion in value by 2022.1 With this growth, the category is experiencing a broadening consumer base that’s no longer dominated by bodybuilders and professional athletes but expanding into a wider group of individuals focused on fitness and healthy living.2 Globally, this expansion is largely being driven by growing numbers of proactive, health-conscious consumers turning to convenient lifestyle solutions—including sports nutrition foods, beverages and supplements—to meet their wellness needs.

Regionally, North America holds the largest market share, and lifestyle changes and rising concern for improving health and wellness are key drivers. However, one of the fastest growing markets for sports nutrition is the Asia Pacific region, set to reach $1.4 billion by 2022 (up from $816 million in 2017).3 This market is largely being driven by Japan and China, which represent over 50 percent of regional sales and together are projected to reach $900 million by 2022. Growth in Asia is attributed to an increase in disposable incomes coupled with rapidly changing approaches to health and wellness, fueling additional demand for sports nutrition products for both lifestyle and recreational users.The Challenges with Capturing DemandWhile the market is ripe with opportunity worldwide, manufacturers struggle to create products that stand out in the marketplace. However, there are strategies that can help capture demand. Manufacturers should source functional ingredients that can deliver proven and targeted health benefits that align with the health and wellness needs of the expanding consumer base. Product offerings should also be formulated with safe, efficacious, and easy-to-formulate ingredients that fit regional consumer interests. Targeted Products Yield OpportunitySimply put, consumers want sports nutrition products that offer specific health benefits. From personalized meal plans to fitness regimens based on metabolic makeup, consumers are demanding products tailored specifically to their needs and lifestyles. In fact, 71 percent of consumers globally find products customized to their individual health needs to be very or somewhat appealing.4

Immune health and recovery are key benefits consumers seek, because they help keep athletes healthy and ready to train. For example, one survey found that improving immune function is a top concern among consumers, with 87 percent expressing an interest in purchasing foods and beverages with immune health benefits.5 Another study revealed that 54 percent of Chinese consumers aged 20-49 would be interested in functional drinks that are formulated for post-workout body recovery, and 20 percent of U.S. consumers state they choose snack, nutrition, or performance bars for exercise recovery.6

In addition to immune health, probiotics can be another opportunity for manufacturers to differentiate products. According to a recent report, 42% of Chinese consumers are interested in functional drinks that assist in digestion.7 In Europe, over 80% of sports nutrition users express an interest in products that support digestive health.8

Research-Supported Ingredients are the Ultimate DifferentiatorConsumer’s continue to expect specific information about health benefits in functional products. Only 15 percent of consumers of nutrition and performance drinks believe the products in the category deliver on promised claims.9 A successful sports nutrition product will hold more value with consumers if it can provide information about how its ingredients work, their safety, and the benefits they provide.

Utilizing a functional ingredient backed by research helps manufacturers overcome the lack of consumer trust related to safety, efficacy and quality. Proven, validated research can be the basis of substantiating marketing messages and building credibility. Ultimately, functional ingredients should be thoroughly researched and clearly convey a benefit-driven message that consumers can understand and trust.

However, It’s important for manufacturers to realize that not every functional ingredient can fit the applications sports nutrition consumers demand. Knowing an ingredient’s formulation strengths and limitations is essential when creating a product that delivers nutritional benefits. This is particularly important as we are seeing a demand for increasingly diverse consumption formats within the sector. Globally, there is growth in the traditional applications of sports bars, powders, and ready-to-drink beverages. However, on-the-go snacks, ready meals, and cereals are also starting to feature sports and performance claims10 Solutions for Formulating Immunity Products With immune health and recovery a top demand, one ingredient that meets the challenge of offering personalized benefits is Wellmune®, a natural food, beverage and supplement ingredient clinically proven to help strengthen the immune system. Backed by over a dozen clinical studies supporting its efficacy, Wellmune is a proprietary baker’s yeast beta 1,3/,1,6 glucan that can help support the immune system of athletes and weekend warriors—keeping them primed for performance no matter their life stage. Wellmune Research Supports Immune Health Benefits and Efficacy Wellmune is unique because of the clinical research that supports its mechanism of action and provides biomarker, safety, and efficacy data demonstrating its effectiveness for athletes and weekend warriors. Peer-reviewed, published clinical studies provide consistent outcomes specific to this particular baker’s yeast beta glucan. From a formulation standpoint, Wellmune is easily added to a variety of foods, beverages and supplements and can withstand normal processing conditions without degrading in efficacy or quality.

Clinical studies with

Research-backed ingredients provide product differentiation in crowded sports nutrition market

Sports nutrition has gone from niche market to mainstream consumption category. In a crowded and competitive market, research-backed ingredients provide products with the added health benefits that consumers demand while giving manufacturers the differentiation necessary to stand out on shelves.

By Dr. Don Cox, Ph.D., R&D Director – GanedenBC30® and Wellmune®, Kerry

marathoners, cyclists and other athletes demonstrate that Wellmune helps athletes stay healthy during and after intense exercise, allowing harder and longer training. In one clinical study with marathon runners, participants taking Wellmune experienced a 40% reduction in upper respiratory tract infection (URTI) symptoms.11

Furthermore, a study with both recreational and elite athletes showed that participants taking Wellmune had less risk of immune system suppression than typically follows high-intensity exercise.12 Finally, another study reported that participants taking Wellmune experienced increased vigor and mental clarity, as well as reduced fatigue, tension, confusion, and URTI symptoms in marathon runners.13 Probiotics Can Deliver Multiple BenefitsWhile probiotics are often linked to digestive health, mounting research shows they can do much more. GanedenBC30® (Bacillus coagulans GBI-30, 6086) is a great fit for the sports nutrition market because it can not only deliver digestive health and immunity support in products but may also enhance protein utilization.14

It is important to note that while appreciation of the benefits of probiotics is growing, there are still a number of misconceptions related to formulation. Many assume that all probiotic strains are the same; however, strains each possess their own distinct characteristics. These unique properties influence safety, efficacy, and suitability for certain applications. As a result, you can’t use the positive benefits or findings of a study on a particular strain to substantiate the health benefits of another. With over 25 peer-reviewed, published studies on its health benefits, GanedenBC30 has long-term safety and efficacy data. GanedenBC30 Research In a key finding for sports nutrition manufacturers, recent studies show that combining one billion colony forming units (CFUs) of GanedenBC30 with one serving of protein (animal or plant) helped to reduce muscle soreness and enhance recovery post-exercise.15 These results are particularly important for manufacturers that target sports nutrition and active adults because protein and recovery are key consumer

needs in this sector.Regarding formulation

challenges, spore-forming probiotics like GanedenBC30 are much more resistant to extremes of pH, heat, cold, and pressure than vegetative probiotic cells, making them a better fit for fortification of everyday foods and beverages. GanedenBC30 is highly stable and offers up to three years of shelf life. It has a natural, protective shell that shields it from stomach acids as well as food-processing conditions like heat, shear, HTST and HPP pasteurization. This increased stability opens up new avenues of

innovation: It can be used not only in chilled dairy products but also in sports nutrition applications such as bars, powders, and sports drinks.Delivering Trust & ValueOverall, it’s vital for manufacturers to select ingredients backed by clinical, published research to help demonstrate product efficacy to consumers. This complements overall product branding and reinforces quality and uniqueness while providing benefit-driven immunity solutions that consumers can trust and value. As the sports nutrition market becomes more diverse, manufacturers can rely on branded functional ingredients to provide the differentiation necessary to succeed in the marketplace.References 1 Euromonitor International, Passport Analysis, Aug 2018.2 Euromonitor International, Consumer Health Sports Nutrition: Drivers and Prospects, July 2017.3 Euromonitor International, Passport Analysis, Aug 2018. 4 GlobalData, Top Trends in Hot Drinks, March 2018.5 GlobalData, Global Consumer Survey, 2015.6 Mintel, Snack, Nutrition and Performance Bars – US, April 2018.7 Mintel, Consumer Attitudes toward Functional Drinks – China, April 2017.8 Mintel, Attitudes Toward Sports Nutrition UK, 20189 Mintel, Nutrition and Performance Drinks, US, March 2018.10 Innova Market Insights, Sports Nutrition: New Performance Platforms, 2017.11 “Baker’s Yeast Beta Glucan Supplementation Increases Salivary IgA and Decreases Cold/Flu Symptomatic Days After Intense Exercise.” (2013) Journal of Dietary Supplements 10:171-183.12 “Baker’s Yeast ß-glucan Supplementation Increases Monocytes and Cytokines Post-Exercise: Implications for Infection Risk?” (2013) British Journal of Nutrition 109:478-486.13 Effect of Beta 1,3/1,6 Glucan on Upper Respiratory Tract Infection Symptoms and Mood State in MarathonAthletes.”(2009) Journal of Sports Science and Medicine 8:509-515.14 Ralf Jäger, Kevin A. Shields, Ryan P. Lowery, Eduardo O. De Souza, Jeremy M. Partl, Chase Hollmer, Martin Purpura and Jacob M. Wilson PeerJ July 2016.15 Jäger R, Shields KA, Lowery RP, De Souza EO, Partl JM, Hollmer C, Purpura M, Wilson JM. (2016) Probiotic Bacillus coagulans GBI-30, 6086 reduces exercise-induced muscle damage and increases recovery. PeerJ 4:e2276

nutraceuticals now 3736 nutraceuticals now

With sports nutrition moving from products only used by elite athletes into the mainstream, including now being found in the supermarket sector this has lead to a demand for cleaner products whilst maintaining product integrity.

Looking at the key trends and demands from the whole food sector including sports nutrition such as sugar reduction, salt reduction, fat reduction and clean alternatives we have looked to expand our Formulating The Future Together portfolio of formulations, and developed a few new ones to showcase the functionality of ingredients to meets these needs. For this we have utilised our Development Kitchen at Brenntag Widnes facility, working on trials as well as bespoke projects based on specific customer briefs.

As a food technologist, I find the opportunities for innovation truly fascinating, as so many truly innovative functional ingredients have been developed specifically to tackle the formulating challenges faced by the industry. It is exciting to be able to spend time with our technical team (Louisa, Taj, Steve and Patrick) on refining new recipes. We have recently worked on number of applications within the food and beverage industry, and completed work on no- bake protein snacks, reduced sugar flapjack, gluten & dairy free cakes, vegan burgers to name but a few.

I would struggle to pick a strong favourite among the many technical projects we have been recently been working on! One brief for instance was to develop individual carrot cakes which are suitable to serve in coffee shop, service stations & restaurants as a healthier, free- from alternative to a traditional carrot cake. The UK coffee culture is booming due to large coffee chains like Starbucks and Costa. Our product development goal was to develop a carrot cake which was gluten free, dairy free and would contain no added sugar. This was a challenging brief as the customer wanted to use gluten free flour, no added sugar, no dairy butter or egg which are the basic ingredients for making a sponge cake. The ingredient we chose, Dow Walocel™, performed exceptionally well helping to give structure to the cake, whilst flavours can be varied according to manufacturer’s brief.

Where a reduction in sodium is required but still the perception of a salty note then use of the PuraQ® Arome products especially in savoury application gives an increased perception of salty savoury notes; these products

In recent years there has been a considerable push to remove those ingredients which are perceived as being chemical or not natural with ingredients which are seen as being cleaner in declaration terms and potentially origin. This drive has come from the retail sector and in particular the supermarkets who feel the consumer wants to see ingredients and names which they understand and can identify as being things they would have at home. This effect has now spread across all sectors of the food industry.

By Stephen Herring, Brenntag UK & Ireland

are natural flavours. Lactic Acid, both in liquid and powder form, gives rise to a pH drop which will assist in slowing microbial growth plus reduce water activity. Lowering the pH impacts the heat sensitivity of bacteria; thus improving the efficiency of any heat processes used. In dairy based drinks lactic acid will enhance flavours but with a neutral acidic dairy taste when compared to other acidulants.

Additionally lactic acid can improve the stability of anthocyanins, and also helps mask the effects of High Intensity Sweeteners (HIS)- a truly multifunctional ingredient. Using HIS products has been an integral part in controlling the calorie level of products but also now as part of the government targets to reduce sugar level & hence the amount consumed. We could recommend a wide range of traditional HIS products such as Acelsulphame K, aspartame, saccharin & sucralose.

Whilst the HIS products are still widely used especially in dry mixes we are seeing an increase in the demand for Stevia. Through our partnership with PureCircle we are able to offer not only those grades of Stevia considered basic such as Reb A97 or SG95, but now a much broader range of single types and blends. Whilst the majority of applications tend to be in the beverage sector, we are now seeing products being used in dry mixes especially the “shake” type products.

With the energy and protein bar sector these products tend to be sugar rich and by using Chicory Root Fibre you are able to achieve good levels of sugar reduction but also the addition of soluble fibre. Our partner Sensus offer a range of products from long chain inulin giving high fibre levels to sweeter oligofructose syrups which can replace glucose based binder syrups by replicating the bulking, functional and textural characteristics offer by sugar based ingredients. Chicory Root Fibre gives you the opportunity to create healthier products while retainig the good texture and great taste. The Sensus range consists of various powder

and liquid forms ranging from 0% up to 60% sweetness. There is an increasing demand for protein fortification which

traditionally has been done using dairy proteins especially in the protein based systems, bars, powder and liquid systems. We have seen a rise in demand for high protein versions of mainstream products such as bread and this has resulted in the demand for vegetable proteins increasing coupled with the rise in demand for both vegetarian and vegan offerings. I would recommend looking at both Soya and Pea Protein for powder & liquid formulations.

As part of the rise of vegan products there has been a demand for products which can replace the functional characteristics of egg, both whole and albumin, and Dow Methocel™ range provides necessary foaming capabilities, structure and moisture migration control.

With sports nutrition products and in particular those aimed at hydration include an electrolyte blend which will include salt (sodium chloride) as well as other key minerals such as calcium, potassium and magnesium. A number of products can be used for fortification of this type of product such as Calcium Lactate, Calcium Lactate Gluconate, Magnesium Gluconate plus Zinc & Ferric Gluconate. In formulations which have citrus flavours it is common to use the salts of citric acid, whilst the use of carbohydrate blends tend to comprise products such as maltodextrin, dried glucose syrup, fructose and dextrose. We would also advise to look at starches, carrageenan, guar gum, locust bean gum and blends for mouthfeel, for instance.

As the bulk in powder blends tends to come from elements with little sweetness once again HIS are used, but as they do not give the same viscosity as sugar then we need to reintroduce “body”. For both powder & liquid systems this can be done very effectively with low to mid viscosity Walocel brand Cellulose Gum from Dow. In addition to using these products for simple viscosity modification they can also be used in dairy based high protein drinks to allow for the use of acidic flavour by protecting the milk proteins from coagulating.

In reviewing both the key trends and the products in the Brenntag range it is clear that by choosing the correct products for the right application we can offer potential solutions to meet the demands being made on the whole food industry both in terms of government driven targets and consumer needs.

For more information please contact Stephen Herring, Brenntag UK & Ireland, on stephen.herring@brenntag.co.uk

Use of Clean label Ingredients to help meet consumer demands and government targets

38 nutraceuticals now nutraceuticals now 39

COMPANY NEWS . . . COMPANY NEWS . . . COMPANY NEWS-

Startup Innovation Challenge: Apply Now! Now accepting submissions for the startup competition at Hi Europe & Ni 2018

Frankfurt/Amsterdam, 31 July 2018 – As part of Hi Europe & Ni 2018, trade show organizer UBM will showcase some of the most promising startups involved in the F&B industry. Applications for the Startup Innovation Challenge are now being accepted and the most promising concepts will be presented live to a professional audience on the first day of the event. The three winners will receive extensive coaching from recognized industry experts.

Startups often exemplify creative pioneers and outstanding innovations, but young companies sometimes lack the budget and support to make an impact on the market. This is why UBM launched the Startup Innovation Challenge in 2016. It gives founders or small new enterprises the opportunity to reach a broad specialist audience and receive valuable advice. The nominees will present their idea or innovation to a jury of experts on 26 November and at Hi Europe & Ni’s Industry Insights Theatre in Frankfurt.

With more than 10,000 visitors and more than 500 exhibitors, the show is the central trade event for healthy functional ingredients for the food and beverage industry. All shortlisted startups will have access to a Startup Lounge, situated in the heart of the exhibition, for the duration of Hi Europe & Ni 2018. This provides a perfect opportunity to meet, network and demonstrate their products to this highly relevant and influential group.

Interested startups must enter one of the following categories before Friday 21st Septembers:• Most Innovative Healthy Food or Beverage Ingredient• Most Innovative Plant-Based Finished Product• Most Innovative Technology or Service Supporting F&B.

The three winners will get individual advice from one of the jury members. In addition, the successful nominees can choose from various special prizes — from a fully equipped stand at Hi Europe 2018 or Fi Europe 2019 to a marketing campaign within the Ingredients Network or access to the “Conciergerie” innovation platform from Presans to intensive consultation at Wageningen University & Research.

Feedback from proven expertsThe jury comprises industry experts, investors and company representatives from well-known corporations in the health ingredients industry. Jury member Thomas van den Boezem is Program Manager at StartLife, an incubator founded by Wageningen University & Research that specializes in coaching startups in the food industry. He says: “Novel food ingredients that make an environmental and health impact are a major focus for us. Wageningen University & Research performs extensive research on this topic; and, as a result, we see more and more startup activity in this space. But, we recognize that other ecosystems in the world often have creative new solutions that we’ve never seen before, so for us this challenge is a great way to stay engaged.” Sandra Einerhand from Einerhand Science & Innovation, who helped to implement the first Challenge in 2016 and has been a jury panel member from the beginning, adds: “The world is in transition: the climate is changing, the population is growing and there is an increased risk of diet-related diseases. So, we have to do something about the way we produce and consume food. One source of innovation is start-up companies and the Challenge is a great way to support them. Receiving valuable feedback about their business model and product, and with valuable prizes on offer, they can both create awareness and use this opportunity to meet or identify potential investors or venture funds.”

To apply, companies should be less than 5 years old and have a solid business plan. The submission must focus on an exceptional new product or service that promotes health and, ideally, already exists as a prototype or service model.

Interested young companies can apply directly at https://startups.figlobal.com or contact sophie.clark@ubm.com for more information.

nutraceuticals now 4140 nutraceuticals now

With a high number of active ingredients, problems including particle size, flow, compressibility, moisture sensitivity, ingredient interaction, content uniformity and quality control (QC) testing can be difficult when producing tablets. There is also the universally problematic issue of sticking, one which can severely impact production. The difference is that pharmaceutical ingredients are often synthetic and are formulated to aid compression, containing formulas that help bind the tablet and generally speaking have less impact physically on the tooling. Nutraceutical ingredients on the other hand, can be hard and difficult to compress. Due to the ‘difficult’ characteristic of vitamins and minerals, tooling can be easily damaged through wear and impregnation.

The traditional solution to these issues is to order more tooling but if the specification is correct in the first place the rewards in increased yield, productivity and reduced equipment downtime can be huge. Get the Design RightSo, let’s look in detail at the specific challenges facing nutraceutical manufacturers. First on the list is the tablet’s design.

To produce robust tablets, the design is an important consideration to start with as it is key to how producible a tablet will be and the quality of the end product. The design can help to reduce wear to tablet tooling, a vital factor when it comes to the abrasive formulations. If a tablet requires the profile to have a deep curve it can very often cause wear on the areas of tooling with the steepest gradient. During the compression stage, the granule must move sideways across this area to form the tablet causing friction. This can grind on the punch tip face removing material over time, leading to problems like capping (when the top of tablet separates horizontally when ejected from the press) and delamination (when the tablet splits apart along layers). By designing in a shallower flatter tablet profile this problem can be reduced, for example by adding a double radius the concave is less, therefore reducing potential friction.

Another design feature which can make a considerable difference is the clearance between the punch tip outer diameter and the die bore. Course granule can penetrate this clearance accelerating wear. When the granule is compressed between the punch and die through lateral movement, it can wear the die bore and punch tip resulting in ringing, clawing and crowning of the punch tip. Having the correct tablet design can help with this issue, but there are also other elements to consider in the prevention of unnecessary tool wear including material and coating. Choose Your Material WiselyThe correct choice of tool steel can make a huge impact on production. The tool material must be balanced to give optimum tooling performance and durability. These properties include abrasion and corrosion resistance, compressive strength, hardness and resistance to chipping and cracking. Tablet punches and dies are the main components to interface with the powders and granules, so they must be metallurgically robust, particularly in nutraceutical production as the wrong selection can accelerate wear. If the wrong choice of tooling steel is used, the compression of abrasive formulations will result in numerous problems in addition to those discussed

above including embossing and breaklines being worn on the tooling resulting in lettering on tablets becoming poorly defined and functionality of breaklines compromised.

Some leading tooling manufacturers are investing in the development of punch and die packages designed specifically for the nutraceutical industry. One example is the new NutraTool® from I Holland which has been designed to combine the correct tool material and coating for the demanding compression requirements of aggressive formulations.

It is important to use a steel that is hard wearing and withstands the effects of abrasion, corrosion and impregnation of hard granules. One example is ESR NutraGrade steel which has been developed and manufactured to I Holland’s own bespoke specifications. ESR, or Electro slag re-melted steel, is refined to give lower non-metallic inclusions and a more consistent structure. This material has a higher chromium content as well as other compositional materials, providing greater wear resistance.

There are other suitable steels on the market including tungsten carbide which offers excellent wear resistance. This material is often used for the dies to prevent wear and deformation of the die bores. It features a high compressive strength with an extremely high wear resistance lasting longer than conventional die steels. It is, however, important to note that this material is very brittle resulting in the premature fracture of punch tips so use on nutraceutical tablet punches can be problematic. Certain formulations can also react with tungsten carbide and cause black spots on the walls of tablets. With this in mind, looking at steel specifically designed for the nutraceutical industry like ESR NutraGrade steel used in NutraTool® would be beneficial.Don’t Forget Your Coat!When it comes to nutraceutical tablet tooling, coating is necessary, giving it an extra layer of resistance to wear. The correct coating will improve the hardness of the tooling preventing abrasive, sharp-edged minerals affecting the tooling which can lead to weight variation, sticking and other issues resulting in the scrapping of the punch.

A resilient coating which includes the benefit of being high in chromium is a must. It should be applied via magnetron-sputtered PVD. This process causes a very smooth (anti-stick) and dense coating to form. I Holland’s NutraCote includes these qualities and is made specifically nutraceuticals in mind. With a hardness rating of around 3000 Hv (est) it is five times harder than the substrate steel. This increase in hardness is possible due to the very thin nature of the coating combined with its controlled elemental composition. This can give dramatic increases in wear resistance extending tool life by over 900% in production case studies.

Importantly the coating is applied using innovative methods. Traditionally, ARC physical vapour deposition (ARC PVD) is used to apply old fashioned chromium coatings. In ARC evaporation a semi-controlled discharge of electrical current is applied to the target in the form of an electrical ARC. This breaks the material from the target, which is then applied to the tool inside a vacuum. This method, however, can cause coating material droplets, larger than is required, to be applied to the tool. These droplets can then break from the coating through abrasion leaving behind defects. It is important to understand how the coating is applied to avoid this.

Don’t Let Aggressive Formulations Affect Your Tablet Production An overriding challenge when it comes to the manufacture of solid dose nutraceutical tablets is the nature of the ingredients found in natural supplements. They are often coarse, abrasive and sometimes corrosive in character. Multivitamins, for example, can contain up to 50 active ingredients and two to eight excipients including coating ingredients. When you compare this to a pharmaceutical formulation which on average contains one to four actives and five to six excipients, it is clear to see that nutraceutical formulations can create production problems due to its complex and aggressive nature.

By Alex Bunting – I Holland Marketing Manager

Magnetron-sputtered PVD, used to apply NutaCote, helps to combat the ‘droplet’ effect. With magnetron sputtering the target material is vaporised by exciting the chromium atoms with a bombardment of gaseous particles. The magnetron sputtering process causes a very smooth (anti-stick) and dense coating to form. This process incurs none of the drawbacks associated with applying hard chrome. Additionally, this method is performed at a lower temperature than other PVD coatings and so are applicable to standard HPG-S steel as the lower temperature does not affect its structure. The Proof is in the NumbersDuring case studies, NutraCote has been shown that when paired with design improvements, and NutraTool® there are huge improvements in anti-abrasive performance leading to much higher numbers of tablets being produced before the punch needs to be replaced.

Using a wear resistant coating is imperative when producing nutraceuticals. An example of this was seen during a trial undertaken by I Holland and a leading nutraceutical manufacturer. An uncoated tool steel and a traditional CrN coating were trialled against I Holland’s NutraCote (PharmaCote RS). After producing 10 batches of tablets (16,300,000) the uncoated steel and Chromium Nitride coated punches showed wear. The specially developed NutraCote was still intact with no wear after this production time.

Following further production of 24 batches, the uncoated tool was removed due to excessive wear and the CrN coating had completely worn off the upper punch and was near to this status on the lower punch. Due to this result, the CrN was removed from the trial. NutraCote however was still intact. Although it was showing some wear around the punch edge, the coating was still protecting the embossing of the tablet.

The final results showed that the uncoated tooling lasted 14 batches, producing 22,820,000 tablets, with the traditional CrN withstanding 20 batches and producing over 32 million tablets. NutraCote lasted over 44 million tablets giving an increase of 93% in life over the uncoated steel used in the past.Make the Right ChoiceEnsuring that the correct choice of tooling is used within the production of nutraceuticals is crucial. The formulations used are habitually abrasive accelerating wear and leading to the premature damage of punches and dies. The results are decreased output and an increase in costs.

Look at the design of the tablet, make sure it is robust and includes the tailor-made properties required to produce a quality end product, then determine the most suitable material and coating combination for the product. Look for tooling which has been specifically designed for the manufacture of nutraceuticals as it will help to withstand the vigours of compressing difficult formula. By choosing the right quality punches and dies problems during manufacture can be prevented.

To find the best solutions to any tableting issues consult with an experienced tooling manufacturer who has the knowledge and innovation to ensure a problem-free quality end product.

Heavy abrasion/impregnation on uncoated tip face

Abraded breakline

Punch tip edge abrasion

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Created in 2009, a SME called ACTIV’INSIDE discovered in several studies that polyphenols are essential molecules acting against cognitive decline. This young company initiated and led a 4-year international R&D project to develop an innovative nutritional solution preventing memory decline: MemophenolTM. The following article describes their innovative approach through this unique ingredient’s story.Nutrition as a key factor to protect our memoryWhatever the age, some lifestyle choices increase the risk of cognitive decline, including smoking, unbalanced diet, lack of physical activity, and stress. Studies show that maintaining good general health habits help prevent cognitive decline. Diet affects our cognitive abilities in youth and impacts whether we develop memory troubles as we age.

Keeping the brain healthy through good nutrition is one important strategy in favor of reducing the risk of cognitive decline(1-3).

Recently, more than 6 epidemiological studies, carried out on 30,000 subjects revealed that individuals consuming a diet rich in essential polyphenols, like flavonoids found in berry fruits exhibit better cognitive function(4-9).

Figure 1: Flavonoid intake and cognitive functions, adapted from Letenneur et al., 2007(4)

However, 85% of US and European, and 64% of Asian adults do not eat enough fruits and vegetables to meet dietary recommendations: their polyphenol intake, especially of specific flavonoids, is not sufficient for preserving optimal cognitive performances and preventing the age-related cognitive decline. In this context, supplementation is of principal interest.

To overcome the lack of science concerning this health topic, the research project NeurophenolTM thus aimed to answer to 3 main questions: Which polyphenols for brain among the 8000 existing in plants? Which mechanism of action? Are they efficient and bioavailable on humans? NeurophenolTM International research programThis program of 4.2 million euros involved 10 academics and industrial partners, as well as 22 scientists, Ph. D. and engineers well recognized for their expertise in the fields of chemistry and biology of polyphenols, neuroscience and engineering of nutritional active ingredients. This consortium aimed to develop nutritional actives rich in specific polyphenols and to substantiate their beneficial and synergistic role on the age-related cognitive decline.

10 scientific studies were conducted according to a pharmaceutical approach:

- In the first part of the research project, a proprietary formulation of extracts from French grape and wild blueberry,

named PEGB (Polyphenolic Extract from Grape and Blueberry) was developed.

- Preclinical studies were performed in the second phase to evaluate the effectiveness of the PEGB specific ratio. Its synergistic effects and mechanisms of action on both bioavailability and neuroprotection were determined on different models. Several dosages were tested to confirm the ratio effectiveness and its safety.

- Finally, a clinical study confirmed PEGB effectiveness on healthy humans. A bi-centric randomized, double-blind, placebo-controlled study on 215 subjects has been performed. A formula adapted to human was born: MemophenolTM.Results of the research project3.1 Which polyphenols for brain?3 specific sub-classes of flavonoids were found to have the greatest potential to reach brain memory areas: anthocyanins, flavonols, especially quercetin and flavanols, especially monomers (catechin/epicatechin). Flavanol monomers have the highest bioavailability with an absorption rate of 45%, compared to that of dimers which not exceed 1%. Their positive action on brain is mainly due to their benefits on microcirculation (10-12) and oxidative stress in the hippocampus (13, 14).Resveratrol, owing to stilbenes sub-class, is able to cross the blood brain barrier and enhance hippocampal neurogenesis and synaptic plasticity (15, 16).Phenolic acids, especially ferulic acid, provide neuroprotective effects on the hippocampus by their antioxidant activity (17).3.2 Where find these essential polyphenols?Grape: the power of monomersParticularly rich in flavanols monomers and resveratrol, grape provides benefits on cognitive function, as reported in clinical studies(12, 18).MemophenolTM formula was developed with carefully selected Vitis vinifera L. varieties, having the best flavanols monomers content. Coming from Champagne and Bordeaux, Chardonnay and Pinot noir are two of the main grape varieties found in MemophenolTM. They contain higher levels of monomeric flavanols when compared to others such as Merlot(19, 20).Blueberry: the “brain berry”Also called “brain-berry” for its clinically proven benefits(21,

22) on both short-term and long-term memories, blueberry contains specific flavonoids and phenolic acids known for their positive action on brain.Wild blueberry (Vaccinium angustifolium A.) was selected for MemophenolTM development for its higher levels of phenolic compounds, especially ferulic acid. Its antioxidant activity is associated with higher concentrations of polyphenols, especially anthocyanins and phenolic acids, compared to cultivated blueberry.3.3 A patented synergistic association of grape and blueberryMemophenolTM justifies its innovative character by its unique ratio. Several doses of grape and blueberry were tested to optimize their synergistic effects on:- Bioavailability: Co-ingestion of both grape and blueberry extracts provides fivefold increase of plasma concentration of blueberry metabolites compared to single extracts administration(23).- Antioxidant activity: combined grape and blueberry extracts

From epidemiological studies to a patented and awarded ingredient. . . the story of Memophenol

TM

developmentInspired by epidemiological studies, MemophenolTM is an innovative formula combining French grape (Vitis vinifera L.) and wild blueberry (Vaccinium angustifolium A.) extracts, rich in selected bioavailable flavonoids. Backed by 1 patent, 10 publications and 2 awards, MemophenolTM is a natural and safe solution to support your cognitive performances.

increase by 20% the neuroprotective effects on human neurons compared to single extracts.

Figure 2: Synergistic action of MemophenolTM patented ratio

on bioavailability (left) and antioxidant activity (right)

This ratio is now internationally patented.3.4 A clinically proven efficiencyMemophenolTM effectiveness was confirmed on humans by a bi-centric randomized, double-blind, placebo controlled clinical study(24). 215 healthy seniors aged from 60 to 70 years old, having normal aged-cognitive decline were enrolled. Before (T0) and after 6 months (T6) of supplementation, 2 cognitive tests from the validated CANTAB battery assessed subjects’ memories: Verbal Recall Memory (VRM) test, evaluating short-term working memory, and Paired Associated Learning (PAL) test, assessing long-term episodic memory.

This study shows that MemophenolTM improves short-term working memory and long-term episodic memory as it:

- Significantly increases the number of words recalled in the supplemented group, compared to placebo, in the total population (Verbal Free recall Recognition memory test, called VRM-FR).

- Improves the episodic memory: MemophenolTM significantly reduces the number of errors at the PAL test on people having a more pronounced cognitive decline at baseline, also called “decliners”.

- Induces a higher urinary concentration of specific flavanols metabolites, traducing a MemophenolTM high bioavailability.

Figure 3: Proprietary clinical results - cognitive age measured according to normative cross-sectional data from total errors at

the PAL testAfter 6 months, clinical results show that “decliners”

supplemented with MemophenolTM regain memory skills equivalent back to 10 years of cognitive age.

Complementary proprietary studiesRecently MemophenolTM has been associated with:• A recent pilot study conducted by ACTIV’INSIDE on 50 French graduated students, from 18 to 25 years old (mean age 23.1), coming from Bordeaux. The study shows that within 15 days only, MemophenolTM supplementation induces booster effects on 3 out of 5 students usually satisfied by their cognitive performances. This effect has been demonstrated to persist in time. At the end of the study, 3 out of 4 students recommend the product.• A recent new 12-year follow-up epidemiological study(25) conducted by Inserm, Activ’Inside and Harvard, was performed on 1329 healthy French people aged 65 years old and above to investigate how a diet rich in polyphenols could prevent cognitive decline. Results show that higher polyphenols consumption reduces the risk of having severe cognitive decline by 50% when comparing the population having the highest intake of polyphenols with the lowest one.What makes MemophenolTM different?MemophenolTM is supported by 1 patent and 10 scientific publications conducted in a pharmaceutical R&D approach, either epidemiological, in-vitro, in-vivo and clinical studies, confirming its effectiveness on memory, with an identified mechanism of action on neurogenesis and synaptic plasticity.

MemophenolTM has been awarded twice internationally as:- BEST INGREDIENT in the “Healthy Ageing” category, at NutraIngredients Awards 2017 (Vitafoods Europe 2017);- BEST INNOVATION in the “Pharmaceutical Product” category at the 2016 French Innovation Corner (organized by BPI France)

ACTIV’INSIDE will be exhibitor at Vitafoods Asia 2018, September 11-12, Singapore. Come to see us booth N10 to know more about our product. On this event, we will present in exclusivity our last quick effect solution against stress, with Safr’InsideTM microtabs, finalist of the NutraIngredients Asia 2018 in the “Botanical product of the year” category.

References: 1. Wheeler MJ. 2017.2. Blumenthal PJSJA. 2016.3. Angevaren M. 2008.4. Letenneur L, Proust-Lima C, et al. 2007.5. Kesse-Guyot E, Fezeu L, et al. 2012.6. Devore EE, Kang JH, et al. 2012.7. Nooyens AC, Milder IE, et al. 2015.8. Rabassa M, Cherubini A, et al. 2015.9. Root M, Ravine E, et al. 2015.10. van Praag H, Lucero MJ, et al. 2007.11. Abd El Mohsen MM, Kuhnle G, et al. 2002.12. Krikorian R, Boespflug EL, et al. 2012.13. Balu M, Sangeetha P, et al. 2005.14. Balu M, Sangeetha P, et al. 2006.15. Harada N, Zhao J, et al. 2011.16. Ramassamy C. 2006.17. Luo Y, Zhao HP, et al. 2012.18. Krikorian R, Nash T, et al. 2010.19. Liang Z, Yang Y, et al. 2012.20. Yilmaz Y and Toledo RT. 2004.21. Krikorian R, Shidler M, et al. 2010.22. Bowtell JL, Aboo-Bakkar Z, et al. 2017.23. Dudonne S, Dal-Pan A, et al. 2016.24. Bensalem J. 2018.

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Obesity rates among children in the Asia Pacific are growing at a rapid rate and will soon pose a serious healthcare threat to countries in the region if no action is taken, according to a recent warning by the United Nations’ Food and Agriculture Organisation (FAO).

Between 2000 and 2016, the number of overweight children under five years old in the region grew by 38 percent, and these children have a higher risk of becoming obese adults and then developing diseases such as Type 2 diabetes, high blood pressure and liver problems, the FAO said in April 2018.

To combat the threat of “diabesity” – a term used to describe the close relationship between Type 2 diabetes and obesity – governments and families need to encourage healthier diets, more active living and stress management exercises, says Dr Naaznin Husein, president of the Indian Dietetic Association’s (IDA) Mumbai chapter.

At this year’s Vitafoods Asia 2018 conference, which will take place on Sept 11 and 12 at Singapore’s Marina Bay Sands Expo and Convention Centre, Dr Husein will deliver a talk titled “Beyond Diabesity and Lifestyle: An Overview of 21st Century Chronic Disease Determinants”, which will outline her research in the field and offer practical tips on reducing diabesity.

The three keys to healthTo eat their way to better health, people should avoid simple carbohydrates such as sugar and include more complex carbohydrates and protein in their diet, Dr Husein said in a recent interview.

“Protein is especially important for Asian populations because a large proportion of people have bodies that are low in muscle mass and high in body fat percentage,” she said. While such people’s weight may be normal for their height, they could have ‘normal weight obesity’, or ‘skinny fatness’, and be at a higher risk of developing metabolic diseases such as diabetes.

“Eggs, lean meats, pulses and nuts are all good sources of protein, and they also create more satiety and improve muscle build-up. Nuts are especially rich in micronutrients and helpful for weight management,” Dr Husein recommended.

“Encouraging an active lifestyle is also crucial. Simple measures such as taking the stairs or walking or bicycling to work make a big difference. Encouraging people to do more of their favourite sports is also effective because they are already engaged in the activities,” she said.

The third prong in fighting diabesity is finding ways to manage stress. When people are stressed, their bodies respond by releasing higher levels of a hormone called cortisol, which gives cells access to fat and glucose, so that they can escape from the danger.

Chronic stress leads to a sustained, higher level of blood sugar that increases the risk of diabetes. Furthermore, stressed people are more likely to eat unhealthily and exercise less. “Yoga, tai chi, meditation and other exercises that emphasise mindfulness definitely help in reducing stress levels,” Dr Husein said.

Making informed choicesTo encourage more people to lead healthier lifestyles, the

Mumbai chapter of the IDA is embarking on a social media campaign that will explain food labels and emphasise the amount of exercise that people should do if they eat various popular dishes.

“It’s hard to tell people not to eat this or that because they will feel deprived. But if you tell them that they will need to walk for two hours to burn off the 550 calories that they gained from eating that slice of cheesecake, they may choose to walk or not eat that cheesecake. It will at least be an informed choice,” Dr Husein explained.

For more than a decade, she has worked with the non-profit Yoga Institute in India to study the effectiveness of lifestyle interventions by tracking, with their consent, the health of about 300 diabetic people who enrolled in its classes.

“We gave them advice about nutrition, exercises and mood management. Many of them who were on insulin are now off it and taking only oral hypoglycaemic drugs because they have achieved better diabetic control. They have done brilliantly, and that’s due to our holistic approach that looked at all of the factors in their lifestyle,” she said.

She summarised: “They also feel calmer and more in control of their lives. This goes to show that if you help people with their choices, and help them to decide what may be better or best for them, they can do very well.”

Attendees to Vitafoods Asia can stay abreast of the latest research into obesity and disease on life stages by attending the Life Stages Theatre, where researchers and functional food developers alike will present their research findings.

The Vitafoods Asia conference and exhibition provide visitors with a rich learning environment to discover all aspects of the development and application of nutraceuticals, through to the branding, packaging and marketing of finished products. At the conference, and at theatres inside the exhibition hall, subject matter experts and industry leaders will present, in-depth, the latest research into nutraceuticals and discuss recent trends or findings at panel discussions.

The fight against diabesityAt Vitafoods Asia 2018, researchers and innovators will convene to discuss the current initiatives in the food industry that are designed to promote optimal population health.

By Dr Naaznin Husein

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