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©2017 NuGEN Technologies Inc.

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Ovation® RNA-SeqSystem V2

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urno ( noG

Trio RNA-Seq

®

www.nugen.com

M01441v1

High sensitivity whole transcriptome analyses

Trio RNA-Seq offers a complete, best in class, whole transcriptomics solution for FFPE, challenging samples and detection of rare transcripts. Designed for robust and reliable transcript analysis, Trio RNA-Seq can help you consistently capture more information from virtually any sample. Trio RNA-Seq integrates the proven technology from the highly published Ovation® RNA-Seq System V2 to provide a complete workflow from total RNA to library.

Why use Trio RNA-Seq?

Trio combines three powerful technologies:

1. Single Primer Isothermal Amplification (SPIA) enables better access to limited and degraded samples.

2. Enzymatic fragmentation and DimerFree library construction allows for more efficient and robust library preparation.

3. Customizable transcript depletion with AnyDeplete maximizes informative sequencing reads from whole transcriptome data.

This powerful combination helps increase sensitivity while maintaining unbiased libraries

with no adaptor dimers, improving detection of rare transcripts and decreasing sequencing costs via higher multiplexing.

Features

Integrated DNase treatment

Unbiased, highly reproducible RNA amplification

Enzymatic fragmentation

Efficient library preparation with unique barcodes for every sample

Customizable transcript depletion after library preparation

Automation friendly

Figure 1 Trio RNA-Seq is a complete workflow, incorporating three proprietary technologies.

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