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BACARA™
New chromogenic medium for detection and enumeration
of Bacillus cereus
Pink/orange coloration
Opacification halo (phospholipase activity)
High selectivity No background flora
No cross reactions
Easy enumeration even with highly
contaminated samples with background flora
High specificity Chromogenic medium utilizing enzymatic activity principle
Easy reading even with difficult matrices
Possibility to enumerate Bacillus from the group cereus on acid or acidifying matrices
Easy protocol (FDA BAM) Results within 24 hours
Spread up to 1 ml on a 90 or 140 mm plate, or use a pour plate
includedincluded
We concur with the AFNOR laboratories and suggest that BAcARA replace MYP as a more rapid method
to recover B. cereus from contaminated food products.
From Tallent et al.: Journal of aoaC International Vol. 95, No. 2, 2012
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16140
v a l i d a t e d
DOC BACARA 9303801 006/US.indd 1 11/06/12 09:23
BACARA™
Ord
erin
gIn
form
atio
n > BACARA™
aEB520100: 20 plates 90 mmaEB120102: 10 plates 140 mmaEB620106: BaCaRa™ Kit (5 bottles 100 ml + suppl.)
> Buffered Peptone Water
aEB610304: 6 x 90 mlaEB610308: 6 x 225 mlaEB910303/4: 4 Dilubag™ 3 litersaEB910305/2: 2 Dilubag™ 5 litersaEB140302: 500g
> Tryptone salt (0.1% Peptone Water)
aEB911493/4: 4 Dilubag™ 3 litersaEB911495/2: 2 Dilubag™ 5 liters aEB141492: 500g
Sample dilution
Incubation period: 24h ± 2h at 30 °C ± 1 °C
Enumeration of typical colonies: pink/orange color + opacification halo
Spread plate
• For a result < 100 CFU/g 0.1 ml on 90 mm BaCaRa plate
• For a result < 10 CFU/g 1 ml on a 140 mm BaCaRa plate
Pour plate
• For a result < 10 CFU/g 1 ml in 18 ml BaCaRa agar 47 °C ± 2 °C
bioMérieux Industry595 anglum Road
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Tel: (800) 634 7656
Fax: (314) 731 8678
bioMérieux Canada, Inc.7815 Henri-Bourassa Boulevard West
St. Laurent (Québec) • Canada H4S 1P7
Tel: (1) 514 336 7321
Fax: (1) 514 807 0015
www.biomerieux-usa.com
9303
801
006/
US/
A ©
201
2 bi
oMér
ieux
, Inc
. • D
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DOC BACARA 9303801 006/US.indd 2 11/06/12 09:24