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BACARA New chromogenic medium for detection and enumeration of Bacillus cereus Pink/orange coloration Opacification halo (phospholipase activity) High selectivity No background flora No cross reactions Easy enumeration even with highly contaminated samples with background flora High specificity Chromogenic medium utilizing enzymatic activity principle Easy reading even with difficult matrices Possibility to enumerate Bacillus from the group cereus on acid or acidifying matrices Easy protocol (FDA BAM) Results within 24 hours Spread up to 1 ml on a 90 or 140 mm plate, or use a pour plate included included We concur with the AFNOR laboratories and suggest that BACARA replace MYP as a more rapid method to recover B. cereus from contaminated food products. From Tallent et al.: Journal of AOAC International Vol. 95, No. 2, 2012 16140 validated

BACARA - bioMérieux · BACARA ™ Ordering Information > BACARA™ aEB520100: 20 plates 90 mm aEB120102: 10 plates 140 mm aEB620106: BaCaRa™ Kit (5 bottles 100 ml + suppl.) > Buffered

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Page 1: BACARA - bioMérieux · BACARA ™ Ordering Information > BACARA™ aEB520100: 20 plates 90 mm aEB120102: 10 plates 140 mm aEB620106: BaCaRa™ Kit (5 bottles 100 ml + suppl.) > Buffered

BACARA™

New chromogenic medium for detection and enumeration

of Bacillus cereus

Pink/orange coloration

Opacification halo (phospholipase activity)

High selectivity No background flora

No cross reactions

Easy enumeration even with highly

contaminated samples with background flora

High specificity Chromogenic medium utilizing enzymatic activity principle

Easy reading even with difficult matrices

Possibility to enumerate Bacillus from the group cereus on acid or acidifying matrices

Easy protocol (FDA BAM) Results within 24 hours

Spread up to 1 ml on a 90 or 140 mm plate, or use a pour plate

includedincluded

We concur with the AFNOR laboratories and suggest that BAcARA replace MYP as a more rapid method

to recover B. cereus from contaminated food products.

From Tallent et al.: Journal of aoaC International Vol. 95, No. 2, 2012

“ “

16140

v a l i d a t e d

DOC BACARA 9303801 006/US.indd 1 11/06/12 09:23

Page 2: BACARA - bioMérieux · BACARA ™ Ordering Information > BACARA™ aEB520100: 20 plates 90 mm aEB120102: 10 plates 140 mm aEB620106: BaCaRa™ Kit (5 bottles 100 ml + suppl.) > Buffered

BACARA™

Ord

erin

gIn

form

atio

n > BACARA™

aEB520100: 20 plates 90 mmaEB120102: 10 plates 140 mmaEB620106: BaCaRa™ Kit (5 bottles 100 ml + suppl.)

> Buffered Peptone Water

aEB610304: 6 x 90 mlaEB610308: 6 x 225 mlaEB910303/4: 4 Dilubag™ 3 litersaEB910305/2: 2 Dilubag™ 5 litersaEB140302: 500g

> Tryptone salt (0.1% Peptone Water)

aEB911493/4: 4 Dilubag™ 3 litersaEB911495/2: 2 Dilubag™ 5 liters aEB141492: 500g

Sample dilution

Incubation period: 24h ± 2h at 30 °C ± 1 °C

Enumeration of typical colonies: pink/orange color + opacification halo

Spread plate

• For a result < 100 CFU/g 0.1 ml on 90 mm BaCaRa plate

• For a result < 10 CFU/g 1 ml on a 140 mm BaCaRa plate

Pour plate

• For a result < 10 CFU/g 1 ml in 18 ml BaCaRa agar 47 °C ± 2 °C

bioMérieux Industry595 anglum Road

Hazelwood, MO 63042 • U.S.A.

Tel: (800) 634 7656

Fax: (314) 731 8678

bioMérieux Canada, Inc.7815 Henri-Bourassa Boulevard West

St. Laurent (Québec) • Canada H4S 1P7

Tel: (1) 514 336 7321

Fax: (1) 514 807 0015

www.biomerieux-usa.com

9303

801

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DOC BACARA 9303801 006/US.indd 2 11/06/12 09:24