Banea Ioana

within hospitals, a proper decontamination of surfaces and hands of the staff is mandatory, considering the need of nosocomial infections prevention Disinfectants – strong bactericidal activity

Used on surfaces Antiseptics – low bactericidal/bacteriostatic activity

Used on hands, lesions

The comparison of antibacterial activity of various antiseptics and disinfectants that are commonly used in hospitals

we analysed the activity of four type of antiseptics

Clorhexin Pliwa derm Iodine Alcohol

four disinfectants Triton rapid Pliwa big extra Roditzin Pliwa lemon fresh

On seven different bacterial species

Serial binary dilutions

(100 μl)

Decontam

inants

(200 μl)

standard CLSI microdilution method. In microtiter plates, binary

dilutions were made of each decontaminant to a final volume of 100 μl.

0.5 McFarland bacterial suspensions

10 μl were inoculated in 9990 μl Muller-Hinton broth.

100 μl were inoculated into each of the plate wells.

incubated at 35°C for 24 hours.

0.5 McFarland 9990 μl M-H

10 μl

100 μl

For each bacterial species and for each decontaminant, the last dilution were no growth was found was noted.

MIC

From the last three dilutions without growth > spot inoculation

MBC

S. aureus E. faecalis E. coliP.

aeruginosaShigella Salmonella Clostridium

Clorhexin 32 4 16 2 16 4 32

Pliwa derm 32 4 4 16 8 8 64

Iodine 128 128 64 64 32 64 256

Alcohol 8 4 4 16 4 8 4

Triton rapid 256 4096 256 256 4096 4096 4096

Pliwa big extra 4096 4096 512 512 4096 4096 4096

Roditzin 256 16 16 32 8 16 256

Pliwa lemon fresh

512 512 128 16 256 256 512

The highest the dilution factor …•The strongest the decontaminant•More sensitive bacteria

Anti

sep

tics

Dis

infe

ctants

Rank Name Type Component 1 Component 2 Componenet 3

1 Pliwa big extra disinfectant amine Quaternary ammonium salt polyether

2 Triton rapid disinfectant amine polieter

3 Pliwa lemon fresh disinfectant alcohol Quaternary ammonium salt

4 Iod antiseptic iodine

5 Roditzin disinfectant amine alcohol, phosphates enzymes, surfactants

6 Pliwa derm antiseptic alcohol

7 Clorhexin antiseptic alcohol clorhexidin digluconate

8 Alcool antiseptic alcohol

Pote

ncy

To disinfectants Pseudomonas aeruginosa Escherichia coli

To antiseptics Pseudomonas aeruginosa Escherichia coli Shigella Salmonella Gram-negative

bacteria

The disinfectants have strong antibacterial activity there are some bacterial species which resist better than

other, especially if these substances are diluted too much.

The non-alcohol based decontaminants are the most potent For the decontamination of skin, other antiseptics than

alcohol are recommended.

The most resistant bacteria are the Gram-negative