BC M514 - Olympus AU400

Cover page ST_CV_1.DOC

M514 M516 AU400

Set Up Manual

Rev. 0500

Technical Support Group Global Business Technical Support Department Beckman Coulter K.K

Revision Date Revision Date Issued 1998.06.05 0100 2000.04.30 0200 2000.09.05 0300 2002.01.31 0400 2009.09.10 0500 2011.01.07

AU400 Set Up MANUAL HISTORY TABLE P. 1

REV. PAGE REPLACE HISTORY 0100 Cover Yes Addition (Rev. 0100)

Cover Yes Addition (Rev. 0200) 0-1 Yes Addition (4-9, 7-7) 1-5 Yes Addition (ME6332 Tray) 1-6 Yes Addition (Name plate) 4-9 Yes Addition 7-1 Yes Revise 7-2 Yes Revise (Page No) 7-3 Yes Revise (Page No) 7-4 Yes Revise (Page No) 7-5 Yes Revise (Page No) 7-6 Yes Revise (Page No)

0200

7-7 Yes Revise (Page No) 0300 1-1 Yes Addition of attention matter

Cover Yes Addition (Rev. 0400) TC Yes Addition (Chapter 10) 1-1 Yes Addition (Spec. of AU403,AU404(M516)) 4-2 Yes Addition (Warning Caution) Modified figures. 4-4 Yes Changed the destination name 4-7 Yes Addition (Instruction) 4-9 Yes Addition (Add memo (Except AU403,AU404))

0400

Chapter 10 Yes Newly created 0500 Cover Yes Company name changed

M514 SET UP MANUAL

TABLE OF CONTENTS

st_tc_01.DOC

1. Set up Preparation.......................................................................... 1-1 to 1-6

2. Set up Flow ..................................................................................... 2-1

3. Unpacking....................................................................................... 3-1 to 3-4

4. Set up Procedure ............................................................................ 4-1 to 4-9

5. Set up personal computer .............................................................. 5-1

6. Processing the heater line of JD.................................................... 6-1

7. Operation Check............................................................................. 7-1 to 7-7

8. Test running ................................................................................... 8-1 to 8-2

10. ISE Set up ....................................................................................... 10-1 to 10-7

0400 0200

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Installation Environment Conditions

Installation Environment Conditions

Site not subject to direct sunlight

Site not minimum amount of dust

Site that is flat (gradient : less than 1/200)

Site with minimum vibration

Site with floor construction that can support approximately 480Kg (4902N)

Places located less than 2000m above sea level

Power and noise Conditions

Site which be located so that 10m water supply ad drain hoses

Site with a power source with minimum voltage fluctuation (+-10%)

Site the switchboard which is located so that a power cable with a length of 10m will reach the system.

A place with no institution sending the signal of vertical polarization of 280 to 300 MHz to the

neighborhood (The police, a fire department, and public institution may be equivalent)

A place with no corrosive gas.

Temperature and humidity conditions The temperature of the installation room should be between 18 and 32C, and the temperature fluctuation

during analysis should be +-2C. (the calorific value of the environments : approx. 2600Kcal/H)

The humidity must be between 40 and 80%RH with no condensation. (Humidity condition for

installation : between 5 and 40 C)

Water Supply and Drainage conditions An aqueduct gate must be within 10 m of the water supply gate of the instrument and the water waste gate

must be under 0.1 m in height. (If the optional forced waste water unit is used, a height of 1.5 m is

required.)

Inner diameter of water waste gate must be greater than 48 mm.

The purity of DI water must be under a conductivity of 2.0 us/cm. (The CAP type 2 level.)

The temperature must be greater than 5 C.

The pressure in the aqueduct must be between 49 and 392 KPa. (0.5 to 4 kgf/cm) (Caution ! : If the water

pressure is greater than 2 kgf, attach a water supply unit.)

Average amount of water required

Unit types AU401, AU402 (M514) : 26.0 L/h (50/ 60Hz)

Unit types AU403, AU404 (M516) : 20.0 L/h (50/ 60Hz)

Maximum instantaneous amount of water required : 0.7 L/min. (50/ 60Hz)

Kind of waste liquid

Concentrated waste liquid (sample + reagent) : 2.0 L/h (including reagent)

Washing waste liquid : 24.0 L/h (Unit types: AU401, AU402 (M514))

Washing waste liquid : 18.0 L/h (Unit types: AU403, AU404 (M516))

1-1

0400 0300

Set up preparation

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Space requirements

As shown in the figure below, this system requires space around it for safe installation and maintenance.

Dimensions of system main units (Unit:mm)

Analyzer Width 1450 x Depth 760 x Height 1210

DPR Width 700 x Depth 700 x Height 1430

Power supply Voltage

USA 110V

Europe 230V

Australia 240V

Asia 110V or 220V

Japan AC100 or 200V

Mono phase 50/60 Hz+-1Hz

Voltage change Within +-10%

Electric power 3.5KVA

Switchboard The switchboard should be located so that the standard power supply cable(10 m) will reach the instrument.

Set up preparation 1-2

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Options There are specific optional device models depending on where the instrument will be used.

No. Name Japan

USA Europe

Asia Australia

Contents

MU4970 M514-OPA01 (1) ISE unit MU4971 M514-OPA02 (1) ISE unit MU4972 M514-OPA03 (1) ISE unit MU4986 M514-OPA04 (1) ISE unit MU4702 M520-OPH02 (2) Forced waste water unit (for CSA) MU4148 M520-OPH01 2 Forced waste water unit (for LVD) MU3351 M240-OPH03 (2) Forced waste water unit MU2535 M240-OPD01 (1) (1) (1) Water supply unit (for CSA) MU0815 M250-OPE01 (1) Water supply unit MU4146 M520-OPE01 (1) Water supply unit (for CSA) MU4147 M520-OPE02 (1) Water supply unit (for IEC) MU1853 M240-OPH01 (1) (1) Forced waste water unit


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Checking the contents of the package

Name Japan USA Europe Asia Australia

ANL main body 1 1 1 1 1

Cable set 1

PC rack 1

Floor plate 4 4 4 4 4

Standard accessories 1 1 1 1 1

Starting kit 1

Package type inner 1 1 1 1 1 outer 1 1 1 1

(Special accessories)

* For information about the main configuration, refer to the Function and Mechanism manual.


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Standard accessories list

No. Name Japan USA Europe Asia Australia MU8092 NE rack W 1 1 1 1 1 11.5 to 16.0 10

pcs/set MU8093 NE rack B 1 1 1 1 1 11.5 to 16.0 MU8094 NE rack G 1 1 1 1 1 11.5 to 16.0 MU8095 NE rack R 1 1 1 1 1 11.5 to 16.0 MU8096 NE rack Y 1 1 1 1 1 11.5 to 16.0 MU8097 NE rack O 1 1 1 1 1 11.5 to 16.0

MV2313 PROGRAM 1 MV2314 PROGRAM 1 MV2315 PROGRAM 1 MV2316 PROGRAM 1 1

MV1764 Communicatio

n cable 1 1 1 1 1

MU8108 Template 1 DW4114 Code set 1 125V, 3A, 3m ZM0634 Cuvette 1 1 1 1 1 10 pcs/ set MU9934 S probe 1 1 1 1 1 MU9958 R probe 1 1 1 1 1 MU9888 Luminous

lamp 1 1 1 1 1

ME3333 Partition 20 20 20 20 20 For 30 ml bottle inside ME4610 Partition 20 20 20 20 20 For 15 ml bottle inside ME4611 Partition 20 20 20 20 20 For 15 ml bottle outside ME4305 Coolant 2 2 2 2 2 MB9963 B hose 2 2 2 2 2 12 x 18 x 10 m MB9961 B hose 2 2 2 2 2 15 x 22 x 10 m MA7528 Band 4 4 4 4 4 MA1198 A letter of

guaranty 1

ME4498 Binder 1 1 1 ME5234 Operators

manual 1

ME6164 Operators manual

1 1

MU9066 Rack ID label 1 1 1 1 1 No. 1 to 20 MU9067 Rack ID label 1 1 1 1 1 No. 21 to 40 MU8051 Rack ID label 1 1 1 1 1 No.1 : 14 pcs

No. 2, 3: 3 pcs

MU9413 Stylus wire 1 1 1 1 1 0.2 ZM0227 Stylus wire 1 1 1 1 1 0.3 ME6332 Tray 1 1 1 1 For GA drain


0200

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Standard accessories list

No. Name Japan USA Europe Asia Australia MB0754 Wrench 2.5 1 1 1 1 1 GB9219 Wrench 1.5 1 1 1 1 1 MU8117 Adapter C 1 1 1 22 pcs/ set ME2272 MU8118 Adapter A 1 1 1 22 pcs/ set ME2175 MU8119 Adapter B 1 1 1 22 pcs/ set ME2176 MU8120 Seal 1 1 1 1 1 Seal 4 types for DC cup

identification MD7304 Bottle 60 1 1 1 5 5 MD7305 Bottle 30 4 4 ME4609 Bottle 15 4 4 MD0884 Cap A 12 12 ZM0062 Sample cup 1 1 1 1 100 pcs/ set ME9382 Name plate 3 German ME9383 Name plate 3 French ME9384 Name plate 1 German ME9385 Name plate 1 French ME9430 Name plate 1 For ODC


0200

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nn Setup flow

Selecting the instllation site Removing the packing material Placing the equipment at the installation site

Leveling the equipment Jacking up the equipment

Wiring from AU400 power Wiring for PC, printer, etc Plumbing for supply and waste water

Pouring coolant (forincubation bath)Coolant for the DB (cooling unit) has been poured at the factorybefore shipment

Setting the master detergent

** Power ON(PC only) Check the BIOS Program install Setting of System Maintenance

(Refer to System Maintenance manual)*** After setting, turn off the power

Process the heater line of the JD portion (to prevent empty burnig)

** Power ON Position check by DIAG After position check attach the consumables Air extraction*** Power OFF

Return to processing for heater line of JD

** Power ON W1/Photocal Water test*** Analysis test

Set up complete

2-1Setup flow

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nn UnpackingllRemoving the outside packaging materials

1) Loosen the band.

2) Remove the L angle.

3) Remove the ceiling lid.

4) Use a pair of pliers to remove the joint securingthe body frame.

5) Loosen the wire securing the body of the AU400to the wood frame.

6) Lift the AU400 off the wood frame.

3-1Unpacking

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nn Opening the packagellRemoving the inside packaging materials

1) Loosen the bands, and remove the vinyl sheet.

2) Remove the grip bar.

3) Remove the all pads to protect the cover.

4) Remove the packaging material for the front door.

5) Remove the screw for fixing the back door.

6) Remove the mouthpiece cover.

Unpacking3-2

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7) Remove the packaging fixed to each unit.

8) Peel tapes fixed to the CB(mirror unit).

9) Remove the vinyl sheet fixed to Float SW.

10) Peel the other tapes.

3-3Unpacking

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nn Standard accessories

Unpacking3-4

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ST04_01

Leveling

1) Position the floor plates under the jacks. (Position the plates squarely.)

2) Jack up the 4 casters at the same time. If a caster isnt in contact with the ground, use a level to check that it is

level.

* Open the front door and place the level on the frame.

When checking with a level, place this instrument on the frame after first opening the front door.

3) Use the jacks to make the instrument level.

4-1

Set up procedure

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Wiring the power supply

Wiring the power supply cable between the AU400 and the switchboard of the facility.

! Warning

1. To prevent a short circuit when repairing or assembling, turn off the main power, and wait at least 10 seconds

before starting work.

2. To prevent electric shocks and short circuits do not touch electric parts and wiring with wet hands.

3. Connect grounding terminals otherwise electric shocks or errors may occur.

1) Remove the door on the rear side of the instrument.

2) Connect the power supply cable in the region labeled A in Fig. A. (The power supply unit is housed here.)

Region A 3) Connect the power supply cable to the switchboard of the facility.

* Check that the voltage of the facility is Live (Hot) or Neutral (Cold) using a tester surely before the power supply

cable is connected.

* Grounding resistance : Less than 100ohm

! Caution

Grounding terminal should be connected to the grounding

terminal its grounding resistance is less than 100ohm.

Set up procedure 4-2

0400

Supply power that

conforms to the

instrument specifications

Connect this terminal to

grounding terminal

(Grounding terminal: less than

100 ohm)

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ST04_01

Wiring for personal computer

Make the following connections to the personal computer.

CRT & PC

CRT Connect to

Power supply [Constant power supply output] for PC

AUDIO IN [LINE OUT] connector for PC

CRT signal [Display] connector for PC

PC and standard accessories

PC Connect to

Power supply Output from JD portion of ANL

If the PC and printer are both connected to the JD

portion, split the power using the table tap.

Keyboard [KEYBOARD] connector for PC

Mouse [MOUSE] connector for PC

PC and standard printer

Printer Connect to

Power supply Output from JD portion of ANL

If the PC and printer are both connected to the JD

portion, divide the power by the table tap.

Printer signal [Parallel] connector for PC

PC and ANL

PC (Communication with ANL) Connect to

Serial connector 1 [COM] connector at JD portion of ANL

Special accessories and others

Option Connect to

Option printer [Parallel] connector at extension board of PC

Touch panel signal [Serial] connector at extension board of PC

Online [Serial connector 2] of PC


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Installation of the personal computer

CRT & PC

CAUTION

1. Do not install the personal computer in an unstable location. There is the danger of injury from dropping.

2. Do not close the air hole. There is the danger of fire from a stuffed air.

3. Do not istall the personal computer at a location where it is exposed to water or other liquids. There is the

danger of erroneous operation or fire.

Connection of the personal computer

CAUTION

1. To prevent electric shocks and short circuits do not touch electric parts and wiring with wet hands.

2. Do not insert the power plug into a receptacle while dust or other foreign matter adheres to the plug.

There is the danger of fire.

3. Insert the power plug positively all the way.

4. Contact all cables correctly. A wrong connection can cause trouble.

Connect the ports of the personal computer according to the following table.

For other connections than the following ones, follow the manual for the personal computer.

PC Connecting Remarks

Serial 1 ANL

Serial 2 (Host computer)

Serial 3 Modem Option

Serial 4 Touch panel (expect for America) Option

Parallel 1 Printer

Parallel 2 Optional printer Option

Set up procedure

0400 4-4

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ST04_01

Plumbing for supply and waste water General diagram

Connecting to supply water hose, concentrated waste liquid waterh hose, washing waste liquid water hose, and exhaust air hose.

1) Connect to the blade hoses to the mouthpiece on the rear face. (See the below figure.)

Concentrated liquid water, Washing waste liquid water : Blade hose 15 x 22

Supply water, exhaust air : Blade hose 12 x 18

Instruction

1. Connect to all the hose of concentrated / washing liquid waste, water supply, and exhaust air because a little water is

drained from the exhaust air hose.

2. Do not fold or pinch a drain hose and an exhaust hose, and keep the hose ends open to air.

3. Do not close up a drain hole in which the hoses are inserted.


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ST04_01

Connecting the deionizer

(1) Connection for MU2535 (M250-OPE01) type

* Allow the deionizer to be connected by an expert distributors.

(2) Connection for MU0815(M240-OPD01) type : for Japan

Connection for MU4146(M520-OPE01) type : for USA

Connection for MU4147(M520-OPE02) type : for Europe

* Allow the deionizer to be connected by an expert distributors.


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ST04_01

Connecting the forced waste water unit and water supply valve

Connecting the forced waste water unit

1. Connecting the hose of the forced waste water unit.

Connect the waste water line from the main body (hose) to the IN connector of the forced waste water,

and connect the hose from the OUT connector to the waste gate of the facility. (The drain hole : 1.5m or

less)

(See Fig. A below.)

Connect the tube from the concentrated waste liquid to one forced waste water unit(1st).

Put the accessory stopcock to the mouthpiece in one forced waste water unit(1st).

Connect the hose from the washing waste liquid and the exhaust air to another forced waste water unit(2nd).

2. Connecting the power supply of the forced waste water unit

Connect the power source line and the signal line to the connector of each JD portion. (See Fig B below)

Instruction

Keep the hose ends open to air.

Connecting the water supply valve (option)

1. G type (solenoid valve type)

Connect the tube from the source of the aqueduct to the IN connector of the solenoid valve, and connect

the tube from the OUT connector to the input line of the deionizer.

Connect the control line of the solenoid valve to the connector of the JD portion. (See the Fig.B below)

* If the resin bomb (cylinder) type deionizer is used, do not connect the solenoid valve to the exit side of the

resin bomb, or the bomb may accidentally burst. Connect it to the entrance side.

2. Mini clear type

Connect the control line of the solenoid valve to the connector of the JD portion. (See the Fig.B below)

Fig. A

Fig. B


0400

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ST04_01

Pouring the coolant

1) Open the cover A. (see the figure below)

2) Remove the stopcock under the cover A, and pour the coolant from the gate.

3) Set the stopcock to the gate.

4) Turn on the environments, and check the coolant level.

* Refer to following pages how to set up the environments.

5) Set the cover A (see the figure below).

Setting the original detergent

1) Set detergent A and detergent B as shown in the figure below.

Set up procedure

4-8

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Installation of Drain tube of incubation bath

1) Drain tube of incubation bath is put in the tray. (ME6332)

2) The tray with the tube is put under the AU400.

* Except Europe (Unit types AU401, AU402(M514)) and AU403, AU404(M516)

Set up procedure

0400 0200 4-9

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nn Setting up the personal computer1. Turn on only the PC. Do not turn on anything else.

2. Setting the BIOS* For detailed setting information refer to the system maintenance manual.

3. Installing the AU400 program* For detailed setting information refer to the system maintenance manual.

4. System maintenance setting* For detailed setting information refer to the system maintenance manual.

5. If the setup of the PC has been completed, turn of it.

5-1Set up personal computer

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st06_01.doc

n The purpose for processing the heater line of the JD portionl A new instrument has not primed for the water in the plumbing line. Therefore if the power is turned on for the

first time, the temperature fuse of the heater for wash water heating may trip. Follow the procedure below toprepare the heater line of the JD portion and prevent the fuse from tripping.

*Note that an error is not issued if the fuse is tripped.n Preparation procedure

1) Detach the heater wire from the terminal table of the JD portion on the rear left side of the ANL. (See thebelow figure A.)

2) Insulate the detached wire with vinyl tape.

6-1Processing the heater line of JD

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n Turning on the powerl If the PC has been set up and the JD portion has been processed, turn on the instrument and check the position

and action of each unit.

1. Air extraction of DI water supply pump1) The front cover opened, and F cover is removed.2) The connector of the DI water supply pump is removed.3) The detergent is put in the Detergent tank. (Detergent diluted to 2%.)4) Turn on the AU400 power.5) Please wait until the DI tank becomes being filled with water.6) The filter is removed from the fixing board.7) The filter is shaken, and water is poured in the pump.8) The connector of the DI water supply pump is connected.9) The FC sequence of DIAG menu is done.10) C insert is removed from C body when doing for a while.11) Water is turned on pushing the point of C insert to a big container.12) C insert is installed in C body.13) The filter is shaken, and sir on the inside is pulled out.14) Afterwards, water is made to often circulate.15) The FB sequence of DIAG menu is done.16) At this time, if the noise dose not do from the DI water supply pump, it is completion.17) F cover is installed.

CAUTIONThe bearing wears out if this work is not done.

7-10200Operation check

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ST07_01.DOC

2. Air extraction of coolant line1) The tube of the circulation pump for GA unit in the back of AU400, and air in the tube is pulled out.

CAUTION1. The error ( INCUBATION BATH TEMP. ERROR etc.) might occur when there is air in the tube.2. Please confirm there is no air in the tube.

0200Operation check7-2

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n Loading the consumablesl Load the sample probe, reagent probe, and mixing bar, by checking the position with the DIAG menu. Do not

load these consumable parts before performing the position check, because the position shift may have beencaused problem.

l The DIAG menu does not have to be used if attaching the adapter for DC unit(the cooling sampler) andrecording paper.

n Position check using DIAG menu

1. Check the horizontal position of the sample probe.1) Check the sample probe alignment in the cuvette. (Fig. A).2) Check the sample probe alignment in the wash pot. (Fig. B).3) Check the sample probe alignment in the sample cup(Rack, DC unit). (Fig. C).4) Check the sample probe alignment in the ISE sample pot. (Fig. D).* Check each position and if necessary, adjust according to the Adjustment manual.

A : Cuvette positionB : Wash pot position

C : Sample cup position D : ISE position

Operation check7-3

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2. Check the horizontal position of the reagent probe.1) Check the reagent probe alignment in the cuvette. (bellow fig. A).2) Check the reagent probe alignment in the wash pot. (bellow fig. B).3) Check the reagent probe alignment in the reagent cup. (bellow fig. C).4) Check the reagent probe alignment in the dilution bottle. (bellow fig. D).* Check each position and if necessary, adjust according to the Adjustment manual.

A : Cuvette positionB : Wash pot position

C : Reagent bottle position C : Dilution bottle position

7-4Operation check 0200

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3. Check the horizontal position of the mixing bar.1) Check the mixing bar alignment in the cuvette. (bellow fig. A).2) Check the mixing bar alignment in the wash pot. (bellow fig. B).* Check each position and if necessary, adjust according to the Adjustment manual.

A : Cuvette position

B : Wash pot position

7-5Operation check 0200

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4. Check the dead volume for the sample probe and reagent probe.l Dead volume at rack position

Type Dead volumeHitachi cup 50 m L or lessACA cup 250 m L or less

f 13 blood collection tube 200 m L or lessf 15 blood collection tube 250 m L or less

l Dead volume at the DC unit position

Type Dead volumeCup for small quantity 30 m L or less

Hitachi cup 50 m L or lessHitachi cup 50 m L or less

Conical cup for STAT 50 m L or lessACA cup 250 m L or less

f 13 blood collection tube 200 m L or lessf 15 blood collection tube 250 m L or less

l Dead volume at the reagent bottle

Type Dead volume15 mL bottle 350 m L or less30 mL bottle 1mL or less60 mL bottle 1mL or less

* Check each position and if necessary, adjust according to the Adjustment manual.

Operation check7-6

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n Air extraction

After making all the position checks, as described on the previous page, recheck the probe and mixing barattachment. Carefully check for looseness in the connections of the sample and reagent probe connections

Check the syringe connection (loose the syringe case or tubes).

1) The sample and reagent dispenser is moved up/down and the air is extracted using the ANL maintenance menu.* If the air isnt extracted, detach the syringe case in the dispenser and extract the air by swaying the syringe by

hand.Air extraction position Menu

Plumbing for sample line Maintenance ANL maintenance S/ R dispenser syringe replacementPlumbing for reagent line Maintenance ANL maintenance S/ R dispenser syringe replacement

2) The mix sequence is executed and the outer wash water of the mix wash pot is filled using the ANL maintenancemenu.

Air extraction position MenuPlumbing for mix outer wash Maintenance ANL maintenance Mixing bar replacement

3) The wash nozzle sequence is executed and the wash water is filled using ANL maintenance.Air extraction position Menu

Plumbing for wash nozzle line Maker maintenance ANL maintenance Replacing Wash Nozzle

CautionThe cuvette may be splashed by water from the nozzle the first time because of air in the tube. In the firsttime, detach the nozzle portion necessarily and accept by the container.Once the air has been extracted, replace the nozzle and check the wash condition by discharging the realwash water into the cuvette.

4) The probe wash sequence is executed and the wash water is filled using ANL DIAG.Air extraction position Menu

Plumbing for clot detection sensor Maker maintenance ANL DIAG Probe wash

If the water test (analysis test) is executed without the air having been completely removed thedata may not be reliable. Be sure to extract all the air.

n Processing the heater line in the JD portion1) If the air has been extracted, turn off the power.2) Execute the processing for the heater line of the JD portion described on page 6-1.

Operation check7-7

0200

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n Executing W11) If processing of the heater line of the JD portion has been completed, turn on the instrument again.2) When the instrument starts, execute W1.* For the W1 action, check the wash water discharge and aspiration. (It is easy to check if the cover of the GA

portion is detached.)

n Executing photocal1) If W1 has been completed, execute photocal by closing the GA portion cover and the main body cover.2) If photocal has been completed, check the cuvette error.

If the average value of the check range is 0.03, there is no cuvette error.

3) If a cuvette error has occurred, check the applicable cuvette and execute photocal again after cleaning orreplacing it.

4) Keep executing until there is no cuvette error.

8-1Test running

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n Water test

l If W1 and photocal have been completed, execute the water test as follows and check the photometry lineperformance.

1) Enter the parameters as follows.2) Prepare degassed water (if it is difficult to obtain, use DI water instead) with P8P (triton 100X) to 0.1%. (88

samples x 4 tests).

NAME 340nm 380nm 480nm 600nmCH R1=2 R1=4 R1=6 R1=8Amount for sample dispensing 2 2 2 2Amount for sample dilution 0 0 0 0Force- multiplier ofsample prior-diluent

1 1 1 1

Amount for R1 dispensing 50 50 50 50Amount for R1 dilution 100 100 100 100Amount for R2 dispensing 0 0 0 0Amount for R2 dilution 0 0 0 0Measurement wavelength (main) 340nm 380nm 480nm 600nmMeasurement wavelength (sub) 410nm 520nm 660nm 800nmAnalysis method END 1 END 1 END 1 END 1Reaction slope + + + +Measurement point 1 1st 1 1 1 1Measurement point 1 Lst 27 27 27 27CAL Type MB MB MB MBFactor 10000 10000 10000 10000

* Others : Set the maximum value, etc.* Name, CH, and the reagent bottle position : Set in position optionally.

3) Set the 88 sample racks in white cups and put DI water in each cup.4) Put the degassed water with triton (made in item 2) into the reagent bottle, and place one in the refrigerator.5) The 4th requisition of the 88 samples is registered in normal test requisition in the routine menu.6) Start analysis.7) The data must satisfy the standard below.

Standard The range of 340/410nm, 380/520nm : less than 15 for all channels.The range of 480/660nm, 600/800nm : less than 10 for all channels.

n Test running

Be sure to perform a water test to check the conforming standard if the photometry and the dispenser system are runningcorrectly.

Test running8-2

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ST10_01

ISE Set up

Set up procedure

1) Power off AU400.

2) Remove covers A , B , C , D , E , F and G. (See Fig-1)

3) Remove the front cover and the side cover.

4) Remove pillars under cover A.

5) Attach MV2190 to the PCB cover and then attach the PCB assy under cover F.

6) Plug in all the connector under cover F to MV2190.

7) Mount the ISE unit at the location of cover A.

Before mounting the ISE unit, please remove the tube (Flesh color tube)

8) Un-bundle connectors bundled under cover A.

10-1

ISE Set Up

Fig- 1

Pillars

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0400

ST10_01

9) Plug in connectors of ISE unit. [132][129][136][130][126] (See Fig-2)

10) Plug in connectors to MV1040. Fix a terminal from connector [122] with screw for fixing PCB.

[111][122][112][133][134][Cable from electrodes]

1. Connector 113 on MV1041 is vacant. (This is for PCB checking at our factory)

2. Power cable No.130 is not using.

11) Mount reagent tray and mouthpiece plate assy. Plug in connectors belong to reagent tray.

[123][124][125] (See Fig-3)

10-2

ISE Set Up

Fig- 2

Fig- 3

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0400

ST10_01

12) Mount SA unit ( Dispenser ) to location A in Fig-4 and plug in connectors.

Mount valve at location B and plug in connector [131].

Mount ISE switches assy at location C and plug in the connector.

13) Insert drain tubes from ISE unit to Y shape connector and fix them with tie lap (SKB band).

Put tubes marked with mark band blue and yellow to mouthpiece plate assy fixed in the previous

step. Put tube marked with mark band 8 to [NC] of valve fixed in the previous step through upper

part of ISE reagent tray.

ISE Set Up

Fig- 4

10-3

Fig- 5

Fig- 6

Cut the grommet.

Pass the tube and cable

through this grommet.

M514 SET UP MANUAL

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ST10_01

14) Put tubes marked with mark band red and 7 . Put the tube with red mark to mouthpiece tray assy and

the other tube with 7 mark to [NO].

15) Put tube remaining from [COM] of valve to SA unit. Close the other hole of syringe head with a cap.(See

Fig-7)

16) Fix all covers removed in the previous steps.

10-4

ISE Set Up

Fig- 7

ISE Set Up

M514 SET UP MANUAL

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Position check

Refer to Adjustment manual.

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M514 SET UP MANUAL

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ISE Calibration

Check calibration

Set ISE reagents and execute prime. Calibrate with the condition of the air is completely out.

Standard

Calibration results of three times shall be in the following value.

Slope value Factor value

Na 38 ~ 68 0.80 ~ 1.20

K 38 ~ 68 0.75 ~ 1.25

Cl -38 ~ -68 0.75 ~ 1.25

CAUTION

Take out the air from the line of samples and reagents.

ISE Set Up

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M514 SET UP MANUAL

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ISE data confirmation Confirm the reproducibility of ISE.

Standard

Execute N=20 for 4 round (N=80). Confirm that each round meets the following standard.

Evaluate with CV value if the measured mean value is more than the mean value below.

Evaluate with SD value if the measured mean value is less than the mean value below.

Mean value SD CV

Na 141 mEq/l 0.85 0.6%

K 4.1mEq/l 0.08 2.0%

Cl 103mEq/l 0.72 0.7%

Used sample

Control serum such as AU control (MS0286, MS0287).

CAUTION

Do not use pooled serum. Correct values may not be obtained.

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Go to Top PageCoverHistory TableTable of ContentsChapter 1 Set up preparationChapter 2 Set up flowChapter 3 UnpackingChapter 4 Set up procedureChapter 5 Set up personal computer Chapter 6 Processing the heater line of JDChapter 7 Operation checkChapter 8 Test runningChapter 10 ISE Set Up

Documents

BC M514 - Olympus AU400