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BD Vacutainer®
CPT™ Mononuclear Cell Preparation Tube
AnticoagulantVolume Draw
Tube SizeOrder Number (60 tubes/case)
Market Status
Sodium Citrate 4 mL 13 x 100 mm 362760 IVD
Sodium Citrate 8 mL 16 x 125 mm 362761 IVD
Sodium Heparin 8 mL 16 x 125 mm 362753 IVD
Sodium Citrate 4 mL 13 x 100 mm 362781 (outside N. America)
IVD/
Sodium Citrate 8 mL 16 x 125 mm 362782 (outside N. America)
IVD/
Sodium Heparin 8 mL 16 x 125 mm 362780 (outside N. America)
IVD/
Available from your authorized BD Vacutainer distributor. For more information, visit www.bd.com
BD Vacutainer® CPT™ Mononuclear Cell Preparation Tube
BD, BD Logo and all other trademarks are property of Becton, Dickinson and Company. © 2007 BD03/07 VS5185-2
To get started, consult your BD Representative.
www.bd.com
BD DiagnosticsPreanalytical Systems1 Becton DriveFranklin Lakes, NJ 07417t: 800.631.0174 f: 201.847.4851
One-step cell separationCAUTION: Handle all biologic samples and blood collection “sharps” (lancets, needles, luer adapters, and blood collection sets) in accordance with the policies and procedures of your facility. Obtain appropriate medical attention in the event of any exposure to biologic samples (e.g., through a puncture injury) since samples may transmit viral hepatitis, HIV (AIDS), or other infectious diseases. Utilize any safety-engineered feature if the blood collection device provides one. Discard all blood collection “sharps” in biohazard containers approved for their disposal.
Make the move today to the only one-step, closed system for collection, cell separation and transport.
Separations are completed, hands-free, in one 20-minute centrifugation.
How to order
MAGENTA HOLDING RULE DOES NOT PRINT!
FOR DIE-CUT TEMPLATE ONLY
BDMD-CPTBroREV-8464.indd 1-2 3/22/07 7:00:02 PM
Anticoagulant
Density Gradient Fluid
Gel Barrier
BD Vacutainer® CPT™ Mononuclear Cell Preparation Tube
Before Blood Collection
After Centrifugation
The BD Vacutainer® CPT™ tube is a one-step, closed system for blood collection, mononuclear cell separation, and transportation. It is an evacuated tube containing an anticoagulant (either sodium citrate or sodium heparin) and a cell separation medium comprised of a polyester gel and a density gradient liquid. The polyester gel forms a stable barrier between the cell layers after centrifugation to allow for transportation to the site where testing is to be performed. All this – achieved in one tube!
The only one-step, closed system for collection, mononuclear cell separation, and transportation
One-step Cell Separation› Separate mononuclear cells in a single step from whole blood
› Standardize cell separation protocol
› Obtain high mononuclear cell viability and yield
› Transport separated cells in the same processed tube
› Reduce risk of specimen contamination
› Reduce biohazard exposure
› For In Vitro Diagnostic use
1.060
Cellquantity
Geldensity1.077
1.0801.070 1.090
Monocytes
Lymphocytes
Basophils
Neutrophils
Erythrocytes
Eosinophils
Density (g/ml)
1.100 1.110
Mononuclear Cell Separation
Plasma
Lymphocytes & Monocytes
Gel Barrier
Erythrocytes & Neutrophils
BDMD-CPTBroREV-8464.indd 3-4 3/22/07 7:00:10 PM
Blood CollectionUse standard venipuncture procedure.
TransportationTo re-suspend cells, gently invert one time before transportation.
CentrifugationGently invert 8 times before centrifugation at room temperature: Heparin: 15 min. Citrate: 20 min. Horizontal rotor with swing-out head and appropriate tube adapters at 1500xg RCF.
Note: For best results, centrifuge within 2 hours after blood collection, but no later than 24 hours after collection!
It’s easy to ProcessBD Vacutainer® CPT™ Tubes
1 2
3 4ResultMononuclear cell separation is completed in one step, in the same tube!
Acceptable blood volume:
8 mL 7 mL 6 mL
Cell Suspension (plasma and
mononuclear cells)Density
Gradient Fluid
Anticoagulated Whole Blood
Gel Barrier
Density Gradient Fluid
Plasma
Lymphocytes & Monocytes
Gel Barrier
Erythrocytes & Neutrophils
Unique Benefits
• Separate mononuclear cells in a single step fromwhole blood
• Obtain high cell viability and yield
• Transport separated cells in the same processed tube
• Reduce risk of specimen contamination andbiohazard exposure
• For In Vitro Diagnostic use
BD Vacutainer®
CPT™ Mononuclear Cell Preparation Tube
Product Description
The BD Vacutainer® CPT™ tube is a closed, one-step system for blood collection, mononuclear cell separation and transportation. It is an evacuated tube containing an anticoagulant (either Sodium Citrate or Sodium Heparin) and a cell separation medium comprised of a polyester gel and a density gradient liquid.
The polyester gel forms a stable barrier between the cell layers after centrifugation to allow for transportation to the site where the assay is to be performed.
Pour en savoir plus
A prélever impérativement avec une unité de prélèvement à ailettes disponible chez Ozyme
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Service Technique : Réactifs : 01 34 60 60 24 - [email protected] - Instrumentation : 01 30 85 92 89 - [email protected] Commande - Clients : 01 34 60 15 16 - [email protected] Nous contacter : www.ozyme.fr/contact
Tubes CPT™ : Séparation des PBMC à partir de sangen 1 seule étape
