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BEZ235, nM Erk1/ 2 HBL-100 C 100 250 100 250 HS578T C 100 250 MDA-MB231 C pErk1/ 2 pAkt GAPDH pS6 Supplementary Figure 3. Effect of BEZ235 on the phosphorylation status of Erk1/2 in three TNBC cell lines. Cells were treated with the indicated concentrations of BEZ235 for 24 hours, and cell extracts prepared. The amounts of dually pErk1/2, Erk1/2, pAkt, pS6 and GAPDH were analysed by Western blotting with the respective antibodies. Supplementary Figure 3

BEZ235, nM

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MDA-MB231. HS578T. HBL-100. BEZ235, nM. C. 100. 250. C. 100. 250. C. 100. 250. pErk1/2. Erk1/2. pAkt. pS6. GAPDH. - PowerPoint PPT Presentation

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Page 1: BEZ235, nM

BEZ235, nM

Erk1/2

HBL-100C 100 250 100 250

HS578TC 100 250

MDA-MB231C

pErk1/2

pAkt

GAPDH

pS6

Supplementary Figure 3. Effect of BEZ235 on the phosphorylation status of Erk1/2 in three TNBC cell lines. Cells were treated with the indicated concentrations of BEZ235 for 24 hours, and cell extracts prepared. The amounts of dually pErk1/2, Erk1/2, pAkt, pS6 and GAPDH were analysed by Western blotting with the respective antibodies.

Supplementary Figure 3

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