BIO-DATA - PROF. DR. MOHAMMAD ASHRAF(Impact Factor 28.55 )

1. PERSONAL DETAILS

Name Prof. Dr. Mohammad Ashraf

Father’s name Ahmed Yar

Date of Birth 3rd July, 1961

N.I.C.No. 31202-6862633-5, Bahawalpur. Pakistan.

Domicile Bahawalpur

Present Position Professor, Department of Pharmacy

The Islamia University of Bahawalpur, Bahawalpur. Pakistan.

Permanent Address 16-17 Khurshid Town behind One Unit Colony, Bahawalpur. Pakistan.

Tel: 062-2280229. Cell: 0301-773-6059.

Email:drashraf_iub@yahoo.com, muhammad.ashraf@iub.edu.pk

2. EDUCATION

Year Exam Div. Subjects Institution

1976 S.S.C. I Math., Physics, Chemistry, Govt. High School,

Physiology Hygiene Satellite Town, Bahawalpur. Pakistan.

1978 F.Sc. II Chemistry, Biology,Physics Govt. Inter College, Bahawalpur. Pakistan.

1980 B.Sc. I Chemistry, Zoology,Botany Govt. S.E. College, Bahawalpur. Pakistan

1984 M.Sc. I Chemistry (Organic & Islamia University, Bahawalpur. Pakistan

Biochemistry)

1990 Ph.D. Biochemistry Manchester University, Manchester, U.K.

1991-93 Post-Doc Biochemistry Manchester University, Manchester, U.K.

3. AWARD OF SCHOLARSHIPScholarship awarded by the Ministry of S & T, Govt. of Pakistan for Ph.D. Studies in U.K. from Sep.

1986 to Dec. 1990 (4-years).

4. MEMBER OF SOCIETIES

1. Member, Society for Experimental Botany, United Kingdom. 1987-93

2. Member, Pakistan Society of Biochemistry and Molecular Biology. Life Time

3. Member, Biotechnology Society of Pakistan. Life Time

4. Member, Pakistan Chemical Society. Life Time

5. CURRENT RESEARCH INTERESTS

1. Enzymology, Inhibitory activity of clinically important enzymes

2. Biological activity, phytochemical analysis and antioxidant activity of various compounds

3. In vivo and in vitro effects of medicines and medicinal plants

6. RESEARCH EXPERIENCE [23 YEARS]

1. Doctoral- [Oct 1986 - Dec 1990] [4-years]

School of Biological Sciences, University of Manchester, Manchester, U.K. Thesis entitled “Biochemical

Aspects of Osmopriming in Leek Seeds”. Work was carried out under the supervision of Dr. C. M. Bray.

2. Post-Doctoral- [Jan 1991 - Dec 1993] [3-years]

School of Biological Sciences, University of Manchester, Manchester, U.K. Project entitled, ‘Flux

Control Analysis of Nitrogen Assimilation in Maize Mesophyll Chloroplasts’. Project was funded by

SERC (U.K.) and executed under the supervision of Prof. Dr. Mike J. Emes.

3. Post-graduate- [Sep 1994 – Todate] [16-years 4 months].

Supervision of dissertation of M.Sc. (Chemistry) students and Ph.D. (Biochemistry) scholars, Department

of Chemistry, MSc & MPhil (Phytomedicine) in the University College of Conventional Medicine,

M.Phil in Pharmaceutical Chemistry, Department of Pharmacy, The Islamia University of Bahawalpur,

Bahawalpur, Pakistan.

4. Visiting Research Fellow [16th Jun – 8th Aug 2004] [7-weeks]

HEJ Research Institute of Chemical Sciences, University of Karachi, Karachi. Work was carried out in

the lab of Prof. Dr. M. Iqbal Choudhary on the screening of various enzyme inhibitors of therapeutic

value and antioxidant activity of synthetic and naturally occurring compounds. 7. TEACHING EXPERIENCE

1. PHARMACEUTICAL BIOCHEMISTRY to B.Pharm./Phar-D Classes [Nov 2000 - Todate] [10 years ]

Introduction; Carbohydrate, Lipid and Protein chemistry and their metabolism; Enzymology; Vitamins

and Minerals; Endocrinology; Purine and Pyrimidine metabolism; DNA and RNA structures and

functions; Basic molecular biology and biotechnology.

2. BIOCHEMISTRY/MOLECULAR BIOLOGY to M.Sc. Classes [Sep 1994-Nov 2000] [6-years]

Introduction; Carbohydrate, Lipid and Protein chemistry and their metabolism; Enzymology; Vitamins

and Minerals; Blood and immunology; Endocrinology; Purine and Pyrimidine metabolism; DNA and

RNA structures and functions; Basic and applied microbiology; Basic molecular biology and biotechnol.

3. PHARMACEUTICAL MICROBIOLOGY to B. Pharm. Classes [Oct 1995-May 2003] [7.5-years]

Introduction; Bacterial classification; Bacterial cell structure and functions; Staining; Cultivation and

growth methods; Study of viruses, fungi and protozoa; Sterilization and disinfection; Immunology;

Microbiology of air, soil, water and milk; Basic pharmaceutical biotechnology.

4. BIOINFORMATICS – To BEMS II-Prof Classes [Sessions 2005-08] [3-years]

Introduction and scope, history of bioinformatics, organization and structure of genomes, physical map of

genome, multiple sequence alignment, neuroinformatics in biology, applications of genome analysis and

genomics, introduction to gene therapy and gene delivery systems, microarray development, clinical

databases, database models, automatic diagnostic systems.

5. Biochemistry – BEMS/BHMS classes in College of Conventional Medicine [Session 2002-2010]

6. Taught courses in BS 4-years Biochemistry and Biotechnology Department at IUB in various

Semesters [2007-todate].

1. Introduction to Biomolecules and Biotechnology

2. Introduction to Fundamentals of Microbiology

3. Microbial Physiology and Genetics

4. Clinical Biochemistry

5. Metabolism of Biomolecules

6. Plant Biochemistry and Molecular Biology

7. Phytochemistry – to classes of MPhil in Phytomedicine [Sessions 2005-08] [3-years]

Introduction and scope of phytochemistry, extraction, separation and isolation of constituents of

medicinal plants, characterization of purified isolated compounds by chromatography, biochemical and

physiological study of phenols, glycosides, volatile oils and resins, saponins, cardioactive drugs, steroids,

alkaloids and tumor inhibitors from plants, Metabolism of fat and fatty acids, terpenes, peptides and

amino acids, alkaloids, glycosides and other secondary metabolites, Plant growth hormones and their

metabolism.

Demonstration of Lab work of above classes of Biochemistry, Pharm, Microbiology and Phytomedicine.

8. Pharmaceutical Chemistry – M.Phil Classes. Teaching following papers to MPhil students in

Pharmacy Dept. [Session 2008-todate].

1. Phytochemistry – Synthesis and Classification of Secondary Metabolites

2. Chemistry of Natural Products – Isolation and Characterization and Bioassays

8. EMPLOYMENT

1. Professor [8th Dec 2007 – Todate] [3-years]

Department of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.

2. Associate Professor [11th Nov 2000 – 7th Dec 2007] [7-years]

Department of Pharmacy, Islamia University, Bahawalpur, Pakistan.

3. Assistant Professor [18th Sep 1994 – 10th Nov 2000] [6-years 2-months]

Department of Chemistry, Islamia University, Bahawalpur, Pakistan.

4. Post-doctoral Research Associate [28th Jan 1991 – 31st Dec 1993] [3-years]

School of Biological Sciences, University of Manchester, Manchester, U.K.

5. Lecturer in Chemistry [14th Oct 1984 – 28th Sep 1986] [2-years]

Govt. College Liaquatpur, Distt. Rahim Yar Khan. Pakistan. [Teaching of FSc classes]

9. COURSE / WORKSHOP ATTENDED

1. Workshop on ‘Computational Chemistry and its Applications’ held on 26th-28th June 2006 in Dr.

Panjwani Centre for Molecular Medicine and Drug Research (PCMD), University of Karachi, Karachi.

2. Workshop on Quality assurance and Hematology at AFIP (Armed Forces Institute of Pathology)

Rawalpindi. Dec. 23rd and 27th 2004.

3. International Workshop on “Conservation and Management of Cholistan Desert Resources –

Challenges and Strategies”, March, 27-28, 2000. I.U.B.

4. Second National Workshop on “Bioassay Techniques”, held in HEJ Int. Centre for Chem. Sci.,

University of Karachi, Karachi (Pakistan) from August 26-28, 1999.

5. Workshop on “Latest Developments in Molecular Biology and Biotechnology”, held at NIBGE,

Faisalabad, (Pakistan) from 24th March to 31st March, 1996.

10. RESEARCH GRANT AWARDED

1. Rs. 1,31,000.oo awarded by the University Research Grant Committee for a period of one year for the

project entitled, “Physico-chemical and osmopriming methods to improve seed germination and growth

of selected flora of Cholistan desert.” 1996-1997.

2. Rs. 99,000.oo awarded by World Wide Fund (WWF-Pak) for one year, for the research project

entitled, “Conservation of endangered flora of Cholistan desert by accelerating their germination and

growth” . 1998-1999. Project No. 50016701. A research assistant had been appointed to conduct the

project under the applicant’s supervision.

3.Rs. 50,000.oo awarded by the University Research Grant Committee for a period of one year for the

project entitled, “Biochemical aspects of accelerated germination of selected flora of Cholistan desert.”

1998-1999.

11. LIST OF PUBLICATIONS Total Impact Factor = 28.55 [In J with Impact Factors]

1. I. Ahmad, B.A. Chaudhary, K.H. Janbaz, M. Uzair and M. Ashraf (2011). Urease inhibitors and

antioxidants from Vernonia cinerascens. Journ. Chem. Soc. Pak. 33(1): 114-117. I.F. = 0.194

2. S. Ahmed, N. Riaz, M. Saleem, A. Jabbar, N. Rehman and M. Ashraf (2010). Antioxidant

flavonoids from Alhagi maurorum. J. Asian Nat. Prod Res. 12(2), 138-143. I.F.= 0.608

3. M. H. Kazmi, E. Ahmed, S. Hameed, A. Malik, I. Fatima and M. Ashraf (2009). Cashmirols A and

B, new lipoxygenase inhibiting triterpenes from Sorbus cashmiriana. Chemistry & Biodiversity, 6,

1471-1476. I.F.= 1.926

4. Saad S.M. Al-Saleh, Samiullah Khan and M. Ashraf. (2009). Biochemical characterization and

some biological properties of the phosphodiesterase I purified from Agistrodon bilineatus venom.

Indian Journal of Biochemistry & Biophysics, 46, .221-229. I.F. = 0.574.

5. S. Ali., S. Yasmine, N. Afza, A. Malik, L. Iqbal, M. Lateef, N. Riaz and M. Ashraf (2009).

Mutiniside, a new antioxidant phenolic glucoside from Abutilon muticum. J. Asian Natural Products

Research. 11(5): 457-464. I.F. = 0.608

6. Saad S.M. Al-Saleh, Samiullah Khan and M. Ashraf. (2009). Biochemical and Biological Properties

of the Phosphodiesterase 1 purified from King Cobra Venom. Journ. Chem. Soc. Pak. 31(2): 292-

303. I.F. = 0.194

7. E. Ahmed, A. Malik, M.A. Munawar, S. A. Nagra, M.M. Ahmed, J. Anwar, M.A. Qazi, A. Sharif,

N. Afza and M. Ashraf (2008). Investigations of antimicrobial constituents from Aerva persica. J.

Chem. Soc. Pak. 30(6): 936-941. I.F. = 0.194

8. E. Ahmed, A. Malik, M.A. Munawar, S. A. Nagra, M.M. Ahmed, J. Anwar, M.A. Qazi, A. Sharif,

N. Afza and M. Ashraf (2008). Antifungal and antioxidant constituents from Silybum marianum. J.

Chem. Soc. Pak. 30(6): 942-949. I.F. = 0.194

9. N. Akhtar, G. Aziz, M. Ahmed, A.U. Madni, M. Ashraf and A. Mehmood. HPLC method for

determination of Famotidine in Human Plasma and its Applications in Bioequivalence Studies .

(2008). Journ. Chem. Soc. Pak. 30(4): 567-570. I.F. = 0.194

10. A.U. Madni, M. Ahmad, N. Akhtar, M. Ashraf and Z.A. Shuja (2008). An improved HPLC method

for the determination of ethionamide in serum. J. Chem. Soc. Pak. 30(3): 449-452. I.F. = 0.194

11. S. Ahmad, M. Ashraf, N. Riaz, M.I. Choudhary G.M. Maharvi, N. Afza, A. Jabbar, K.H. Janbaz and

M.S.Khan. (2008). Salsolide, a new p-hydroxyphenylglycol derivative from Salsola baryosma.

Journ. Chem. Soc. Pak. 30(1): 110-112. I.F. = 0.194

12. M. Zabta, M. Ashraf, T. Sharif and A. Saeed (2007). Partial purification and characterization of

alkaline phosphatase from goat kidney. J. Chem. Soc. Pak. 29(5); 509-513. I.F. = 0.194

13. M. Imran Anjum, E. Ahmad, A. Jabbar, A. Malik, M. Ashraf, M. Moazzam and M.A. Rasool.

(2007). Antimicrobial constituents from Fagonia cretica. Journ. Chem.Soc.Pak. 29(6): 634-639.

I.F. = 0.194

14. M. Saleem, M.S.Ali, S. Hussain, A. Jabbar, M. Ashraf and Y.S. Lee. (2007). Marine natural

products of fungal origin. Natural Products Reports. 24:1142-1152. I.F. = 9.202

15. Z. Nizami, Sajid Hussain, A. Malik and M. Ashraf. (2007). A new Ursane-type triterpenoid from

Salvia santolinifolia. Turk. J. Chem. 31, 495-501. I.F. = 0.760

16. M.H. Kazim, E. Ahmed, S. Hameed, A. Malik and M. Ashraf. (2007). Isolation and structural

determination of sorbinol A and B, new triterpenes from Sorbus cashmariana by 1D and 2D NMR

spectroscopy. Magnetic Resonance in Chemistry. 45: 416-419. I.F. = 1.612

17. L. Iqbal, M. Lateef, S. Ali, N. Riaz, G.M. Maharvi, M. Ashraf and N. Afza. (2007). Antioxidant

activities of tetraketones derived from 5,5-dimethylcyclohexane-1,3-dione. Journ. Chem. Soc. Pak.

29(1):51-54. I.F. = 0.194

18. M.A. Yawer, E. Ahmed, A. Malik, M. Ashraf, N. Afza and M.A. Rasool. (2007). New

Lipoxygenase inhibiting constituents from Calligonum polygonoids. Chemistry & Biodiversity, 4(7):

1578-1585. I.F. = 1.926

19. Ashraf M., F.H. Nasim, E. Ahmad, M. Unis, M. A. Rehman, S. Akhtar, N. Naz, N. Anwar and F.N.

Roy (2006). 'Determination of serum nitrate and nitrite contents by Cd-Zn reduction method. Journ.

Chem. Soc. Pak. 28(5): 501-504 . I.F. = 0.194

20. S. Ahmed, M. Ashraf, A. Jabbar, K.H. Janbaz, M. S. Khan, A.H. Gilani, and M.I. Choudhary

(2006). Pharmacological screening of Salsola baryosma. J. Chem. Soc. Pak. 28(1): 82-83. I.F. =

0.194

21. S. Ahmed, G.M. Maharvi, M. Ashraf, N. Riaz, N. Afza, K.M. Khan, M. S. Khan, A. Jabbar, and

K.H. Janbaz. (2006). Phytochemical studies on Salsola baryosma. J. Chem. Soc. Pak. 28(2): 176-

178 I.F. = 0.194

22. E. Ahmed, M. Imran, A. Malik and M. Ashraf (2006). Antioxidant activity with flavonoidal

constituents from Aerva persica. Arch. Pharm. Res., 29(5): 343-347. . I.F. = 1.159

23. S. Hussain, E. Ahmed, A. Malik, A. Jabbar, M. Ashraf, M. A. Lodhi and M. I. Choudhary (2006).

Halosterols A & B, Chymotrypsin inhibitory sterols from Haloxylon recurvum. Chem. Pharm. Bull.,

54(5), 623-625. I.F. = 1.223

24. S. Hussain, E. Ahmed, A. Malik, S. Ferheen, A. Jabbar, M. Ashraf, M. A. Lodhi and M. I.

houdhary (2006). Recursterols A and B, Chymotrypsin inhibiting sterols from Haloxylon recurvum.

Polish Journal of Chemistry. 80: 409-415. I.F. = 0.483

25. Ashraf, M., F.H. Nasim and N. Akhtar. (2000). Changes in electrical conductance of leachates from

wheat seeds pretreated in water and sodium chloride. Pak. J. Bot., 32(1):115-119. I.F. = 0.520

26. Nasim, F.H., T.F. Shahzadi and M. Ashraf (1997). A cold shock during shock during imbibition

improves germination of Acacia nilotica seeds. Pak. J. Botany, 28(2): 183-189. I.F. = 0.520

27. Ashraf, M. and C.M. Bray (1993). DNA synthesis in osmoprimed leek seeds and evidence for repair

and replication. Seed Science Research, 3: 15-23. I.F. = 1.608

28. Bray, C.M., M. Ashraf, P.A. Davison and R.M. Taylor (1993). Molecular Markers of Seed Quality.

In: Come, D. and Corbineau, F, eds., Basic and Applied Aspects of Seed Biology, vol. 3., Paris

(France): ASFIS. pp. 887-896. Chapter in the Book.

29. Bray, C.M., P.A. Davison, M. Ashraf and R.M. Taylor (1989). Biochemical changes during

osmopriming of leek seeds. Annals of Botany, 6: 185-193. I.F. = 3.501

LIST OF PUBLICATIONS – in Journals without Impact Factor [Total 32 Papers]

1. Nasim, F.H., M. Ashraf, Q.I. Sheikh, T. Kokab and Q. Shaheen (1987). Distribution of ABO and Rh

Blood groups in the residents of Bahawalpur. J. Pak. Medical Asso., 37(1): 7-8.

2. Akhtar, H., F.H.Nasim, Q.I. Sheikh and M. Ashraf (1987). Distribution of ABO and Rh Blood

groups in the residents of Sahiwal. J. Pure & Applied Sci., 6(2): 7-9.

3. Nasim, F.H., Aslam, M. and M. Ashraf (1995). Effects of priming treatments on germination of

Acacia nilotica seeds. Hamdard Medicus, 38(2): 116-123.

4. Ashraf, M. and C.M. Bray (1995). Biochemical aspects of osmopriming in seeds. I. RNA-

polymerase activity in leek seeds. Pak. J. Biochem. & Mol. Biology, 28(1-2): 179-187.

5. Rehman, M.A., F.A. Khan, M. Ashraf and Z. Hussain (1996). Chemical analysis of urinary stones in

patients of Bahawalpur division during 1994-95. The Professional Med. J., 3(3): 1-5.

6. F.H. Nasim, M. Aslam and M. Ashraf (1997). Chemical changes during priming and germination of

Acacia nilotica seeds. Scientific Khyber, 10(2): 63-72.

7. Ashraf, M. T.A. Khokhar and F.H.Nasim (1997). Biochemical studies during early growth of Acacia

nilotica. J. Pure & Applied Sci,., 16(2): 5-11.

8. Ashraf, M., R. Shabana and F.H. Nasim (1997). Effect of physico-chemical treatments on the

germination of Panicum antidotale Retz. seeds. Pak. J. Plant Sci, 3(2): 65-72.

9. Ashraf, M. and S. Nisar (1998). Germination patterns and electrolytes leakage during imbibition of

intact and ‘naked’ seeds of Salvadora oleiodes. Pak. J. Biol. Sci., 1(4): 287-290.

10. Ashraf, M. and M.M. Hussain (1998). Changes in amino acid and carbohydrate contents in leachates

of pre-imbibed wheat seeds. Pak. J. Biol. Sci., 1(3): 148-151.

11. Ashraf, M., N. Akhtar, F. Tahira and F.H. Nasim (1999). Effect of NaCl pretreatment on the

germination and emergence of seven cultivars of wheat seeds. Pak. J. Biol. Sci.: 2(4), 1594-97.

12. Yasmin, I., Ashraf, M. and F.H. Nasim (1999). Germination behaviour and electrolytes leakage of

seeds of some plants of Cholistan desert. Pak. J. Biol. Sci., 2(4): 1545-47.

13. S. Rashid, M.F.Qureshi and M. Ashraf (1999). Proximate analysis and antibacterial activity of

Heliotropium crispum. Pak. J. Biol. Sci., 2(3): 720-21.

14. S. Nisar and M. Ashraf (1999). Effect of drying treatments on the germination and imbibitional

leakage of Salvadora oleiodes seeds. Pak. J. Biol. Sci., 2(2): 445-48.

15. Ashraf, M., S.M.Muyassir Boukhari, M. Shoukat and M. Arshad Azad (1999). Physico-chemical

analysis of soils of Cholistan desert. Pak. J. Biol. Sci., 2(2): 430-433.

16. Abdul-Rehman, M., M. Ashraf and F. A. Khan. (1999). Measurement of serum T3, T4 and TSH for

the diagnosis of thyroid dysfunction by employing ELISA methodology. J. Ayub Med. College.,

11(1): 14-16.

17. S. Rashid, Shahid Aqeel and M. Ashraf (1999). Proximate analysis and antibacterial activity of

Glinus lotoides Linn. Hamdard Medicus, XLII (4): 37-39.

18. Ijaz A., S. Rehman, W. Yawar, M. Ashraf, N. H. Syed and Abdul Rashid (1999). Blood magnesium

levels in males and females of varying age. Pak. Armed Forces Med. J., 49(2): 112-113.

19. S. Rashid, M. Ashraf, S. Bibi and R. Anjum (2000). Antibacterial and antifungal activities of

Launaea nudicaulis (Roxb) and Launaea resedifolia (Linn). Pak. J. Biol. Sci,. 3(4): 630-632.

20. S. Rashid, M. Ashraf, R. Anjum and S. Bibi (2000). Insecticidal and cytotoxic activities of Launaea

nudicaulis (Roxb) and Launaea resedifolia (Linn). Pak. J. Biol. Sci. 3(5): 808-809.

21. S. Rashid, M. Khalid, M. Ashraf and M. Arshad (2000). Proximate analysis and antibacterial activity

of Cressa cretica. Hamdard Medicus, XLIII(3): 32-35.

22. Nasim, F.H., Ahmed, M., Ashraf, M., Chaudhry, M.S. and Khan, M.S. (2001). Germination patterns

of Cenchrus and Panicum spp. of Cholistan desert: Effect of temperature. Pak. J. Plant Sci.,6(1-2):

71-79.

23. Ashraf, M. M.M. Hussain and F.H.Nasim. (2001). Loss in germinability of heat-treated wheat seeds

is associated with reductions in electrical conductance of their leachates. Pak. J. Biol. Sci,. 4(2): 121-

123.

24. Ashraf, M., F.H.Nasim and M.M. Hussain (2001). Efflux of inorganic ions in leachates of wheat

seeds. Online J. Biol. Sci., 1(1): 1-3. www.pjbs.org/ojbs.htm

25. Farooq, M., M. Tayyib, T. Tasneem, G.A. Sirhindi, A. Ditta, M. Ashraf and N.A. Choudhary (2003).

An experimental study of coagulation profile in albino rats fed on canola oil supplemented with

atherogenic element for a period of six weeks. Annals K.E. Med. College, 9(3): 221-223.

26. Ashraf M., S. Mukhtar and F.H. Nasim (2004). Effect of -irradiation on imbibitional leakage,

germination and growth of wheat seeds. Int. J. Biol. Biotechnol., 1(4): 563-572.

27. Ashraf M., M.S. Kalati, F. Errum and F.H. Nasim (2004). Effects of artificial seawater treatments on

imbibitional leakage and germination of wheat seeds. Int. J. Biol. Biotechnol., 1(4): 573-579.

28. Munir, A., M. Tayyib, M. Farooq, M. Ashraf, A. Rashid (2004). An experimental study of albino

rats fed on Nigella sativa and aspirin for four weeks duration: Determination of coagulation profile.

Annals K.E. Med. College, 10(2): 121-122.

29. Najam Uddin, M. Tayyib, A. Ditta, M. Farooq, M. Ashraf and N.A. Choudhary (2004). An

experimental study of albino rats fed on canola oil supplemented with atherogenic element:

Determination of tissue lipid profile. Pak Postgrad Med J, 15(1): 25-27.

30. M.A. Khan, M. Tayyab, M. Ashraf, A. Ditta, N.A. Choudhary, M. Farooq and A. Ali. (2005).

Changes in serum lipid profiles of albino rats fed on canola oil supplemented with atherogenic

elements: 18 weeks study. Annals K.E. Med. College, 11(1): 5-7.

31. S. Ahmed, M.Ashraf, A. Jabbar, K.H. Janbaz, M.S. Khan, N. Rahman and M. Hussain, (2006).

Biological activity of Dipterygium glaucum. Pak. J. Biol. Sci., 9(6): 1173-74.

32. Saad S.M. Al-Saleh, Samiullah Khan and M. Ashraf. (2009). Purification of Phosphodiesterase 1

from Cerastes vipera venom, Biochemical and Biological Properties of the purified enzyme. Proc.

Pakistan Acad Sci. 46(1): 1-12.

12. ABSTRACTS PUBLISHED/WORK PRESENTED IN CONFERENCES

1. F.H. Nasim, R. Khalil, A. Sumreen, M. Shafiq and M. Ashraf (2009). Nickel metal uptake and metal-

specific stress alleviation in a perennial desert grass Cenchrus ciliaris. Paper presented in International

Conference in July 2009 in Turkey by First author.

2. F.H. Nasim, S. Iqbal, M. Afzal, M. Saleem, M.S. Chaudhary and M. Ashraf (2008). Cholistan desert

bacterial isolates producing phthalate derivatives. Paper presented (as co-author) in the 9 th Biennial

Conference of the Pak Soc for Biochem and Mol Biology held at Arid Agriculture Univ, Rawalpindi, on

Dec 17-19, 2008.

3. E. Ahmed, M. Imran, M.Ashraf and Abdul Malik (2006). Paper ‘Flavanoidal constituents of Aerva

persica’ presented in 6th International and16th National Conference of Chemical Society of Pakistan held

in B.Z.Univeristy. Multan in April 06-08, 2006.

4. F.H. Nasim and M. Ashraf (2005). Isolation and characterization of biotechnoloically important

bacteria from Cholistan desert. Work presented in National Conf Biochm Mol Biol, Karachi University,

Karachi. 7-9 Jan 2005.

5. M. A. Khan, M. Tayyab, A. Ditta, M. Ashraf, (2004). Serum lipid profiles in albino rat fed on canola

oil supplemented with atherogenic element for prolonged period. Work presented in Annual Conf Pak

Assoc Path held at PIMS Rawalpindi. 24-26 Dec. 2004.

6. Rashid, S., M. Ashraf and M.A. Sheikh (1999). Proximate analysis and antibacterial activity of some

plants of Cholistan desert. In the 10th Nat. Conf. Chem. Soc. of Pak. held at Quaid-e-Azam University,

Islamabad, 26-28 Oct. 1999.

7. Ashraf, M. and S. Mukhtar (1998). Imbibitional leakage, germination and early growth of gamma-

irradiated wheat seeds. In the 9th Nat. Conf. Chem. Soc. of Pak. held at University of Karachi, Karachi,

6-8 Dec. 1998.

8. Ashraf, M. and C.M. Bray (1997). Seed Osmopriming: A tool to understand germination mechanisms.

In the 4th Nat. Conf. of Pak. Soc.of Biochem. and Mol. Biol. held at NWFP Agriculture University

Peshawar, (Pak.), April, 7-10,1997.

9. Nisar, N. I. Yasmin, M. M. Hussain and M. Ashraf (1997). Germination behaviour of Capparis

decidua, Prosopis cineraria and Salvadora oleiodes. In the 8th Nat. Conf. Chem. Soc. Pak. held at

PCSIR Lab. Complex, Lahore (Pak), 2-4 Sep.1997.

10. Yasmin, I. S. Nisar and M. Ashraf (1997). Effects of various physico-chemical treatments on the

germination performance and electrolytes leakage of some plant species of Cholistan desert. In the 4th

Nat. Conf. of Pak. Soc. Biochem. and Mole. Biol. held at NWFP Agriculture University, Peshawar, (Pak),

April, 7-10, 1997.

11. Akhtar, N. F. Tahira, M. M. Hussain and M. Ashraf (1997). Effects of various seed pretreatments on

the germination performance and electrolytes leakage of wheat seeds. In the 4th Nat. Conf. of Pak. Soc.of

Biochem. and Mol. Biol. held at NWFP Agricultural University Peshawar, (Pak.), April, 7-10,1997.

12. Ashraf, M. and C.M. Bray (1995). Biochemical aspects of osmopriming in seeds. I. RNA-polymerase

activity in leek seeds. In 3rd Meeting of the Pak. Soc. Biochem. and Mol. Biol. held at Lahore (Pak.),

April, 1995.

13. Ashraf, M., A.Tobin and M.J. Emes (1993). Flux control analysis of primary nitrogen assimilation in

maize mesophyll chloroplasts. In ‘Plastid Preview’ on Sep. 15-16, 1993, held in the University of Cardiff,

Wales (U.K.).

14. Ashraf, M., A.Tobin and M.J. Emes (1993). Flux control analysis of primary nitrogen assimilation in

maize mesophyll chloroplasts. In SEB (Society for Experimental Botany) Meeting held in Rothemsted

Expt. Station, Harpenden (U.K.). Sep. 1-3, 1993.

15. Bray, C.M., P.A. Davison, M. Ashraf and R.M. Taylor (1993). Molecular Markers of Seed Quality.

In 4th Int. Workshop on Seeds held in France, July, 1992.

16. Bray, C.M., P.A. Davison, M. Ashraf and R.M. Taylor (1989). Biochemical aspects of seed

osmopriming. In The Third Int. Workshop on Seeds, a NATO Advanced workshop held at

Williamsburg,Virginia (USA), from August, 6 - 11, 1989.

13.SUPERVISION OF POST-GRADUATE (M.SC.) THESES [27]

1. Evaluation of antioxidant activities of various herbs and herbal formulations.

Mohammad Ramzan. 2008.

2. In vitro determination of radical scavenging activities of some homoeopathic medicine.

Faiza Shakeel. 2008.

3. Effects of various homoeopathic medicines on the biochemical and haematological parameters in

smokers and non-smokers. Syed Muhammad Umer. 2008.

4. Changes in glutamine synthetase activity and protein contents during germination of pretreated wheat

seeds. Farha Riaz. 2000.

5. Variations in guaiacol-peroxidase activity during germination of pre-treated wheat seeds.

Talat Noureen. 2000.

6. Biological activity of some desert plants. Syeda Sabica Naqvi. 2000.

7. Antioxidant activities of some desert plants. Uzma Hafeez. 2000.

8. Some aspects of carbohydrate metabolism during germination of wheat seeds. M. Tahir Bhutta. 2000.

9. Starch metabolism during germination of bajra (Pennisetum typhodium) seeds. M. Zubair. 2000.

10. Determination of nitrate and nitrite in serum of healthy men. Shamim Akhtar, 1999.

11. Determination of nitrate and nitrite in serum of healthy women. Farhat Nasim Roy, 1999.

12. Determination of nitrate and nitrite in cerebrospinal and ascitic fluids. Nusrat Naz, 1999.

13. Determination of nitrate and nitrite in serum and saliva of smokers and non-smokers.

Nosheen Anwar, 1999.

13. Imbibitional leakage of sodium chloride pretreated wheat seeds. Fiza. Erum , 1998.

14. Effect of artificial sea water (ASW), microwaves and UV-pretreatments on the

germination performance and imbibitional leakage of wheat seeds. M. Sabir Kallati, 1998.

15.Germination patterns and imbibitional leakage of gamma-irradiated wheat seeds.

Sajida Mukhtar, 1998.

16. Physico-chemical analysis of soils of Cholistan desert. S. Muyassar Hussain Bukhari, 1998.

17. Determination of nitrate and nitrite in serum, saliva and urine. Muhammad Unis, 1998.

18. Proximate analysis and antibacterial activity of Heliotropium crispum. M.Farhan Qureshi, 1998.

19. Proximate analysis and antibacterial activity of Glinus lotoides. Muhammad Shahid, 1998.

20. Proximate analysis and antibacterial activity of Cressa cretica. Muhammad Khalid, 1998.

21. Imbibitional leakage of pre-treated wheat seeds. Muhammad Munir Hussain, 1997.

22. Germination patterns and imbibitional leakage of intact and ‘naked’ seeds of Salvadora oleiodes.

Sarvat Nisar, 1997.

23. Germination profiles and imbibitional leakage of seeds of Capparis decidua and Prosopis cineraria.

Izzat Yasmin, 1997.

24. Effects of various treatments on the germination performance of seven different cultivars of wheat.

Nasim Akhtar, 1996.

25. Physico-chemical treatments and biological studies of six different cultivars of cotton.

Tahira Firdous, 1996.

26. Physico-chemical treatments and biological studies in accelerating germination of Panicum

antidotale. Rukhshanda Shabana, 1995.

27. Biochemical studies during germination and growth of Acacia nilotica.

Tasnim Akhtar Khokhar, 1995.

14. SUPERVISION OF Ph.D./M.Phil.THESES [PhD = 2; MPhil = 3]

1. “Nitrate Toxicity in Animals”. M. Abdul Rehman. Ph.D. Thesis. Ph.D. Degree was awarded

in 2003.

2. ‘Exonuclease (phosphodiesterase) from snake venoms, isolation, purification, biochemical

characterization and some biological studies’. By Samiullah Khan. PhD Thesis submitted in

August 2008. Evaluation is in progress.

3. Acetylcholinesterase and NADH oxidase inhibitory activities of some medicinal plants. Khalil

Ahmed Ansari. 2008.

4. Investigations on the inhibitions of lipoxygenase and NTPDase activities of some medicinal

plants. Syed M. Ali Shah. 2008.

5. Identification of various classes of secondary metabolites from plants of Cholistan desert by

chromatography methods. Kiran Shafiq 2009.

6. Inhibition studies of some enzymes of therapeutic importance. Iftikhar Afzal. 2011. Thesis

submitted.

7. High throughput screening of compounds with potential biological activity. Bushra Hussain.

2011. Thesis submitted.

8. Comparative study of the determination of some anticancer drugs by various analytical methods.

Muhammad Munawar Hayat. 2011. Thesis submitted.

15. TECHNIQUES EMPLOYED DURING PH.D. AND POST-DOCTORAL TRAINING AND DURING INDEPENDENT RESEARCH

1. Use of radioisotopes (H3, P32, C14) in labelling experiments, use of ultracentrifuge machine.

2. Isolation and purification of nuclei, DNA, chloroplasts and mitochondria. Estimation of

DNA, RNA and proteins.

3. Agarose and polyacrylamide gel electrophoresis, plasmid isolation, Southern blotting,

hybridisation, autoradiography, etc.

4. Enzyme assays e.g., Nitrate reductase, Nitrite Reductase, Glutamine Synthetase, Glutamate

Synthase, ATP synthetase, DNA-polymerase, RNA-polymerase, DNA-ligase., peroxidase,

catalase, amylase, -glucuronidase, urease, lipoxygenase, tyrosinase, xanthine oxidase,

acetylcholinesterase, butyrylcholinesterase etc.

5. Use of Clark type Oxygen Electrode & assay of Photosystem-I, Photosystem-II & electron

transport chain. Use of inhibitors of these systems.

6. Determination of ATP, ADP and AMP by Luciferin-luciferase method.

7. Hydroponic growth of plants at various levels of nitrate in the medium

8. UV-visible spectrophotometry, Flame photometry, Chromatography

9. Determination of nitrate/nitrite in plasma, ocular fluid, urine etc.

10. Basic methods of staining and growth and cultivation of bacteria.

11. Biological assays: antifungal, antibacterial, insecticidal, antioxidant, cytotoxic, phytotoxic

activities of extracts/compounds of various plants.

12. Use of computer, Word, Excel (graphic package), SPSS (statistical package), Harvard

Graphics, Chem Office, Grafit, EZ-Fit, etc.

16. OTHER ASSIGNMENTS

1. Principal, University College of Conventional Medicine, [3-years] 18th Oct 2006-Nov 2009

Faculty of Pharmacy and Alternative Medicine,

2. Part-time Teaching (& demonstration of practicals) of Medical Biochemistry in

College of Conventional Medicine to classes of

1. BEMS (Bachelor of Eastern Medicine and Surgery) 2000-2010

2. BHMS (Bachelor of Homeopathic Medical System) 2000- 2010

3. Part-time Teaching (& demonstration of practicals) of 2005-2008

Phytochemistry to MPhil students of Phytomedicine

4. Part-time teaching in BS 4-years Biochem/Biotechnology Program 2007-todate

5. Member, Admission Committee Department of Chemistry 1997-1999.

6. Member, University Medical Committee 2000-2001.

7. Member, Departmental/CCM Fee Concession/Scholarship Committee 2001- 2009

8. Member, Admission Committee Department of Pharmacy 2000-2004

9. Coordinator, M.Phil (Phytomedicine) session 2006-2008

10. Member, University Entry Test Committee 2006-2007

11. Member, Advisory Committee in BEMS & BHMS (UCCM) 2006-2009

12. Member, Board of Studies, Pharmacy Department 2001-todate

13. Member, University Academic Council 2008-todate

14. Member, Advance Studies and Research Board 2008-todate

15. Member, University Equivalence Committee 2008-todate

16. Member, Board of Studies in Biochem/Biotechnology Program 2008-todate

17. Member, Examination Committee, M.Phil/PhD Pharmacy Dept. 2009-todate

18. Member, University Senate 2008-todate

19. Member, House Allotment Committee 2011-todate

20. Member, University Auction Committee 2011-todate

LIST OF PUBLICATIONS – in Journals without Impact Factor [32]

33. Nasim, F.H., M. Ashraf, Q.I. Sheikh, T. Kokab and Q. Shaheen (1987). Distribution of ABO and

Rh Blood groups in the residents of Bahawalpur. J. Pak. Medical Asso., 37(1): 7-8.

34. Akhtar, H., F.H.Nasim, Q.I. Sheikh and M. Ashraf (1987). Distribution of ABO and Rh Blood

groups in the residents of Sahiwal. J. Pure & Applied Sci., 6(2): 7-9.

35. Nasim, F.H., Aslam, M. and M. Ashraf (1995). Effects of priming treatments on germination of

Acacia nilotica seeds. Hamdard Medicus, 38(2): 116-123.

36. Ashraf, M. and C.M. Bray (1995). Biochemical aspects of osmopriming in seeds. I. RNA-

polymerase activity in leek seeds. Pak. J. Biochem. & Mol. Biology, 28(1-2): 179-187.

37. Rehman, M.A., F.A. Khan, M. Ashraf and Z. Hussain (1996). Chemical analysis of urinary

stones in patients of Bahawalpur division during 1994-95. The Professional Med. J., 3(3): 1-5.

38. F.H. Nasim, M. Aslam and M. Ashraf (1997). Chemical changes during priming and

germination of Acacia nilotica seeds. Scientific Khyber, 10(2): 63-72.

39. Ashraf, M. T.A. Khokhar and F.H.Nasim (1997). Biochemical studies during early growth of

Acacia nilotica. J. Pure & Applied Sci,., 16(2): 5-11.

40. Ashraf, M., R. Shabana and F.H. Nasim (1997). Effect of physico-chemical treatments on the

germination of Panicum antidotale Retz. seeds. Pak. J. Plant Sci, 3(2): 65-72.

41. Ashraf, M. and S. Nisar (1998). Germination patterns and electrolytes leakage during imbibition

of intact and ‘naked’ seeds of Salvadora oleiodes. Pak. J. Biol. Sci., 1(4): 287-290.

42. Ashraf, M. and M.M. Hussain (1998). Changes in amino acid and carbohydrate contents in

leachates of pre-imbibed wheat seeds. Pak. J. Biol. Sci., 1(3): 148-151.

43. Ashraf, M., N. Akhtar, F. Tahira and F.H. Nasim (1999). Effect of NaCl pretreatment on the

germination and emergence of seven cultivars of wheat seeds. Pak. J. Biol. Sci.: 2(4), 1594-97.

44. Yasmin, I., Ashraf, M. and F.H. Nasim (1999). Germination behaviour and electrolytes leakage

of seeds of some plants of Cholistan desert. Pak. J. Biol. Sci., 2(4): 1545-47.

45. S. Rashid, M.F.Qureshi and M. Ashraf (1999). Proximate analysis and antibacterial activity of

Heliotropium crispum. Pak. J. Biol. Sci., 2(3): 720-21.

46. S. Nisar and M. Ashraf (1999). Effect of drying treatments on the germination and imbibitional

leakage of Salvadora oleiodes seeds. Pak. J. Biol. Sci., 2(2): 445-48.

47. Ashraf, M., S.M.Muyassir Boukhari, M. Shoukat and M. Arshad Azad (1999). Physico-

chemical analysis of soils of Cholistan desert. Pak. J. Biol. Sci., 2(2): 430-433.

48. Abdul-Rehman, M., M. Ashraf and F. A. Khan. (1999). Measurement of serum T3, T4 and TSH

for the diagnosis of thyroid dysfunction by employing ELISA methodology. J. Ayub Med.

College., 11(1): 14-16.

49. S. Rashid, Shahid Aqeel and M. Ashraf (1999). Proximate analysis and antibacterial activity of

Glinus lotoides Linn. Hamdard Medicus, XLII (4): 37-39.

50. Ijaz A., S. Rehman, W. Yawar, M. Ashraf, N. H. Syed and Abdul Rashid (1999). Blood

magnesium levels in males and females of varying age. Pak. Armed Forces Med. J., 49(2): 112-

113.

51. S. Rashid, M. Ashraf, S. Bibi and R. Anjum (2000). Antibacterial and antifungal activities of

Launaea nudicaulis (Roxb) and Launaea resedifolia (Linn). Pak. J. Biol. Sci,. 3(4): 630-632.

52. S. Rashid, M. Ashraf, R. Anjum and S. Bibi (2000). Insecticidal and cytotoxic activities of

Launaea nudicaulis (Roxb) and Launaea resedifolia (Linn). Pak. J. Biol. Sci. 3(5): 808-809.

53. S. Rashid, M. Khalid, M. Ashraf and M. Arshad (2000). Proximate analysis and antibacterial

activity of Cressa cretica. Hamdard Medicus, XLIII(3): 32-35.

54. Nasim, F.H., Ahmed, M., Ashraf, M., Chaudhry, M.S. and Khan, M.S. (2001). Germination

patterns of Cenchrus and Panicum spp. of Cholistan desert: Effect of temperature. Pak. J. Plant

Sci.,6(1-2): 71-79.

55. Ashraf, M. M.M. Hussain and F.H.Nasim. (2001). Loss in germinability of heat-treated wheat

seeds is associated with reductions in electrical conductance of their leachates. Pak. J. Biol. Sci,.

4(2): 121-123.

56. Ashraf, M., F.H.Nasim and M.M. Hussain (2001). Efflux of inorganic ions in leachates of wheat

seeds. Online J. Biol. Sci., 1(1): 1-3. www.pjbs.org/ojbs.htm

57. Farooq, M., M. Tayyib, T. Tasneem, G.A. Sirhindi, A. Ditta, M. Ashraf and N.A. Choudhary

(2003). An experimental study of coagulation profile in albino rats fed on canola oil

supplemented with atherogenic element for a period of six weeks. Annals K.E. Med. College,

9(3): 221-223.

58. Ashraf M., S. Mukhtar and F.H. Nasim (2004). Effect of -irradiation on imbibitional leakage,

germination and growth of wheat seeds. Int. J. Biol. Biotechnol., 1(4): 563-572.

59. Ashraf M., M.S. Kalati, F. Errum and F.H. Nasim (2004). Effects of artificial seawater

treatments on imbibitional leakage and germination of wheat seeds. Int. J. Biol. Biotechnol., 1(4):

573-579.

60. Munir, A., M. Tayyib, M. Farooq, M. Ashraf, A. Rashid (2004). An experimental study of

albino rats fed on Nigella sativa and aspirin for four weeks duration: Determination of

coagulation profile. Annals K.E. Med. College, 10(2): 121-122.

61. Najam Uddin, M. Tayyib, A. Ditta, M. Farooq, M. Ashraf and N.A. Choudhary (2004). An

experimental study of albino rats fed on canola oil supplemented with atherogenic element:

Determination of tissue lipid profile. Pak Postgrad Med J, 15(1): 25-27.

62. M.A. Khan, M. Tayyab, M. Ashraf, A. Ditta, N.A. Choudhary, M. Farooq and A. Ali. (2005).

Changes in serum lipid profiles of albino rats fed on canola oil supplemented with atherogenic

elements: 18 weeks study. Annals K.E. Med. College, 11(1): 5-7.

63. S. Ahmed, M.Ashraf, A. Jabbar, K.H. Janbaz, M.S. Khan, N. Rahman and M. Hussain, (2006).

Biological activity of Dipterygium glaucum. Pak. J. Biol. Sci., 9(6): 1173-74.

64. Saad S.M. Al-Saleh, Samiullah Khan and M. Ashraf. (2009). Purification of Phosphodiesterase

1 from Cerastes vipera venom, Biochemical and Biological Properties of the purified enzyme.

Proc. Pakistan Acad Sci. 46(1): 1-12.