Intrinsic Signal Optical Imaging Remove skin/muscle, thin
skull. Mechanical Movement of C2 Whisker. 5 deflections at 5 Hz.
Illuminate with 630 nm light. Measure change in remitted light.
Grinvald, Lieke, Frostig, Gilbert, and Wiesel, Nature, 1986.
Whisker Stimulator Lens Ron Frostig, UCI
Slide 11
Hemodynamic Response stimulation Initial Dip Overshoot
(BOLD)Undershoot 1 mm 630 nm light (deoxy Hb) t ~ 1 st ~ 4 st ~ 7
s
Slide 12
Spatial Frequency Domain Imaging (SFDI) Rd (fx)Rd (fx) Spatial
Frequency, f x (mm -1 ) a, s ' Cuccia et. al., Opt Lett, (2005)
Cuccia et. al., JBO (2009) 1 0 photon density depth Tissue
Structured light SFDI Implications: 1)Optical Property Mapping
1)Depth-Resolved Imaging Tissue: a low-pass spatial filter
Slide 13
Hyperspectral SFDI L. Gao, R. T. Kester, and T. S. Tkaczyk,
Opt. Express 17, 12293-12308 (2009). Full images (350 x 350 pixels)
of 46 Wavelengths at 5 Hz. Close up Photo of Mapper Soren Konecky
Tomasz Tkaczyk
Slide 14
Intrinsic Signal Tomograpy depth Black & White: Baseline
Color: Functional Change increase decrease 10.5 -0.5
Slide 15
Biomedical Optics Before Chance (B.C.) Light Source Detector
cuvette pre-1987
Slide 16
Biomedical Optics Chance Era (C.E.) No Cuvette (too expensive)
Abundant supply of body parts Time-shared laser sources (off time
saves $$) RF electronics = Radio Shack
Slide 17
Grand Unified Theory of Britt Supply Demand
Slide 18
New Unit of Measure: 1 BCU = 100 mW/cm 2 ~Max exposure
intensity for biotissue damage BCU: Max intensity At the limit