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Well come

Shakir Mahmud

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Biochemical Decomposition of Urea

Performed by

Md. Abdullah-Hel- Kafi Student No. 0202040Shakir Mahmud Student No. 0202041

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Scope of work To increase affectivity of urea in soil To reduce amount of UREA from industrial

waste water in a economical way. Use of microorganism in urine management.

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Introduction

Biochemical decomposition of urea Urea as Fertilizer Remove urea in waste water Manage waste of livestock

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Literature review (con’t) Reaction involved

Enzyme Urease Ammonia Monooxygenase

Urea Ammonia Ammonium

Nitrate Nitrite

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Literature review (con’t) Fat of products in urea decomposition

Fat of ammonium ion Converted to nitrates by autotrophic organisms by the

process known as nitrification. Absorbed by higher plant or Utilized by the micro flora population

Fate of nitrate Absorbed by plant Utilized by the micro flora

reduction of nitrate to nitrite. ion of nitrite to hydroxylamine reduction of hydroxylamine to ammonia reduction of

hydroxylamine to ammonia protein synthesis

Converted molecular nitrogen by Denitrification

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Literature review (con’t)

Urea plant waste water treatment Thermal hydrolysis and steam stripping Biological treatment

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Literature review (con’t)Involving Micro Organisms

Micro organisms involved in urea decomposition-

1. Bacteria 2. Fungi Two type of bacteria- A. Ammonifying bacteria.B. Nitrifying bacteria.

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Literature review (con’t)

Organisms characteristic Urobacter Ammonia-Oxidizing Bacteria Nitrite-Oxidizing Bacteria

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Experimental work

Comparative analysis of amount of decomposition of urea with various Substances Without seeds Normal bed Crushed urea Green leaf in soil Saw dust in soil

Study of five different sample bed consists of Kachuripana, sawdust, bagassae, sugar with soil, only soil. Kachuripana Sawdust Bagasass Glucose with Soil Soil

Determination concentration of urea in urea solution through calibration curve.

Behave with different pH in day to day Study of the rate of hydrolyzation of urea in Glass Column in the

presence of bacteria day to day

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Calibration curve for urea Hydrolyzed

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Result Bio organisms highly increase the

urea conversion Organic matter no effect on

decomposition. Glucose increases ammonification but

have no effect on nitrification. Activity of urobacter good at sharply

basic medium(pH6.5-7.5) but rate of volatilizaton large in basic medium

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Result (con’t)Effect of pH

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Result (con’t)Effect of pH (con’t)

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Result (con’t)Reaction rate of urea with day.

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Conclusion If the soil is fertilized in accordance with normal

practice there is therefore a risk of wasting more than 60% of the fertilizer, which leads to economic losses and serious environmental consequences .

Biological treatment of waste water have great acceptance for its low cost and easy maintain. The method involving thermal hydrolysis and steam stripping to remove ammonia from the condensate recycles urea.