Biodisc-proposal Kirthi Student (2)

8/4/2019 Biodisc-proposal Kirthi Student (2)

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Dt. 27.08.2011

From,

Shaik Khasim Beebi [email protected]

Associate Professor

Department of Biotechnology

Gitam Institute of Technology

Visakhapatnam 530 045

To,

Member Secretary

Prof. T. V. Krishna Reddy,

Andhra Pradesh State Council of Science and Technology

12th floor, Eastern Wing,

Gagan Vihar, M.J. RoadNampally, Hyderabad 500 001.

Sir,

Sub: Application for Student Project Programme-APCOST- Reg.

I am herewith submitting the proposal for the student project programme titled

Bio-disc An economic diagnostic tool for multiple

diseases for Andhra Pradesh State Council of Science andTechnology. Please consider the application and do the needful.

Thanking you sir,

Yours sincerely

(Shaik Khasim Beebi) Principal


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Application for Student Project Proposal

Project Title

Bio-disc An economic diagnostic tool for

multiple diseases

Submitted by

Divya Kriti, Dwarsingh Kushan, Bollem

Rajakumar, Shaik Mohammed Saifuddin Malik

ToANDHRA PRADESH STATE COUNCIL OF SCIENCE & TECHNOLOGY

HYDERABAD

DEPARTMENT OF BIOTECHNOLOGY

GITAM INSTITUTE OF TECHNOLOGY

GANDHI INSTITUE OF TECHNOLOGY AND MANAGEMENT

VISAKHAPATNAM 530045

September, 2011

FORMAT OF PROPOSAL


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1. Name of the College / Department : Gitam Institute of Technology, GITAMUniversity, Department of Biotechnology

2. Name(s) of the Students : a) Divya Kriti

b) Dwarsingh Kushanc) Shaik Mohammed Saifuddin

Malikd) Bollem Raja Kumar

2. (a) Year of study : 2011-2012

3. Name of the guides : Dr. Shaik Khasim BeebiMr. R. Satyanarayana

4. Title of the Project : Bio-disc

An economic diagnostic tool for multiplediseases

5. Project Identification : New Project

6. Has the similar project been carried out at your college: No

7 Importance and Justification of the Project : Annexure I

8. Objectives of the Project : Annexure II

9. Project work methodology : Annexure III

10. Description of the project : Annexure IV

11. Time schedule : Annexure V

12. Budget : Appendix - II

Signature of Guides

(Smt Sk Khasim Beebi) (Mr. R. Satyanarayana)

Signature of HOD Signature of Principal

(Prof. R Sinha) (Prof. D. Prasada Rao)


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Annexure-I

Importance and Justification of the project

To design a low cost multiple diagnostic tool which can diagnose multiplediseases, simultaneously, without compromising efficiency and sensitivity.The diagnostic tool will be reusable and will be able to diagnose multiplepatients, thus, saving a lot of money and effort without the problem of wastedisposal.

In short, the device will be useful for people who find hospitals costly orwant accurate results within minutes. We will start with 2 diseases that areTuberculosis and Diabetes.

The reason to opt these diseases is that we still dont have a highly accuratediagnostic tool for tuberculosis in the initial stages and the occurrence ofdiabetes increases the risk of tuberculosis to a greater extent.

The main feature of this bio-disc will be the option for reusability which willeliminate the necessity of purchasing the same bio-disc again and again asdifferent people will be able to use it.

This project is highly needed because the device designed will beeconomical, environment friendly and available for all the classes of peoplein the society.


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Annexure-II

Objectives

1. To introduce a low-cost polymer or composite material that is well suited

for the bio-disc and can bring down its cost.

2. Designing of the bio-disc using micro-fluidic channels.

3. To check and modify the proposed reusability concept.

4. To study the techno-economic feasibility of the bio-disc for commercial

production.

5. To introduce the bio-disc in society.


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Annexure-III

Project work methodology

Methodology involves following steps

1. Various mechanisms for introduction of blood to interaction of antigens withantibodies, detection of the intensity or amount of interaction, its analysis,display of results and reusability will have to be formulated.

2. Then these proposed bio-discs are to be designed, which will requirevarious mechanical softwares such as CATIA, etc. The design is to be keptsimple but effective.

3. Determination of the materials to be used is the next step for manufacture ofthese bio-discs. The materials have to be bio-compatible, non-reacting and

non-toxic with a smooth surface on which microfluidic channel system canbe implemented.

4. Next step is to determine ways of commercializing this product.


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Annexure-IV

Description of the project

Bio-disc is a small-scale device, analogous to Integrated Circuits, used to

analyze organic molecules associated with living organisms. It is capable of

performing rapid, small-scale biochemical reactions for the purpose of

identifying antigens, gene sequences, environmental pollutants, airborne

toxins, or other biochemical constituents. This will be basically a point of

care clinical diagnostic element.

The major characteristics of this bio-disc will be its ability for multiple

diagnosis and reusability.

The biochip consists of probes (DNA, RNAi, proteins fragments,)

represented by a point on a support (chip). These probes bind very

specifically the targets (complementary gene fragments, proteins or specific

ligands) present in the biological samples to test. The contact between the

probes with their targets is executed due to the hybridization phenomenon and

can be highlighted by fluorescent detection. The target identification and the

signal quantification are then made using various detection systems and the

results are then validated on the statistical level and interpreted in a biological

context.

The principles involved could be either the use of microarrays or the use of

microfluidics. But here we will usethe concept of micro-fluidics and micro-

level behavior of fluids.

The first step will be to formulate a design for introduction and multiplechanneling of the blood. Easy and proper channeling of the blood will be

based on efficient use of the buffer which will dilute the blood and increase its

volume, thus facilitating an easy channeling. Another method can be the use

of mechanical systems such as vertical channels, air bladders or pressurized

N2 production systems.


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The next step will be to design a setup for interaction of monoclonal

antibodies present in biodisc reservoir and antigens present in the blood.

Then methods for the detection and analysis of the interactions need to be

designed which can easily be done through the change in the intensity of

fluorescence. Now, we will formulate some mechanisms for reuse of the

antibodies present and design accordingly. This will be the special feature of

the project.

Next step will be the scaling of the designed model to check its efficacy and

performance at every level.

The ultimate step will be the production of this bio-chip after which we willbe able to go for patenting and commercialization.

Annexure-V

Time schedule


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Appendix - 2

Project Cost Estimate

NAME OF THE INSTITUTION : Gitam Institute of Technology,

Department of Biotechnology


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TITLE OF THE PROJECT : Bio-disc An economic diagnostic tool for multiplediseases

Items

Cost

1. Materials to be used Rs.20,000/-

2. Sample collection Rs.5,000/-

3. Scaling and production Rs.10,000/-

3. Computer expenses, if any Nil

4. Travel Rs.1000/-

5. Other project costs

a) Report preparation Rs. 500/-b) Miscellaneous Rs. 500/-

----------------

Total: Rs.37,000/-

Signature of the Guides

Documents

Biodisc-proposal Kirthi Student (2)