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7/30/2019 BIOE 410 Term Paper Presentation
1/18
Johnson
Huynh and
Antonio Bunce
INCREASING THE LIFETIME
OF ARTIFICIAL LUNG
DEVICE BY INCREASINGTHE BIOCOMPATIBILITY OF
PDMS
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ARTIFICIAL LUNG DEVICE
[Joseph Potkay et al., Lab on a Chip, RSC Publishing, 2011]
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DEVICE STRUCTURE
[Joseph Potkay et al., Lab on a Chip, RSC Publishing, 2011]
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DEVICE STRUCTURE
Air
PDMS Gas Diffusion
Membrane
Blood
PDMS Channel
PDMS Channel
Front View Side View
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Only hours
Heparin and EDTA already used in blood
Thrombus formation occurs in large channels and at size
changes
Non-coated tubing used to introduce blood to device
DEVICE LIFETIME
[Joseph Potkay et al., Lab on a Chip, RSC Publishing, 2011]
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Use of Dermatan Sulfate in combination with
Heparin to functionalize surface
Use of fibronectin to endothelialize high
aspect ratio channels
Surface Modification through single ionirradiation
METHODS OF INCREASING
BIOCOMPATIBILITY
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BIOACTIVE HEPARIN ON PDMS
MICROFLUIDIC CHANNELS
[Sara Thorslund et al.,Colloids and Surfaces B: Biointerfaces, Elsevier, 2005]
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USE OF DERMATAN SULFATE TO
INCREASE THROMBIN INHIBITION
[Douglas Tollefsen, Progress in Molecular Biology and Translational Scienece, Elsevier, 2010]
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Mucopolysaccharidoses
Inhibit coagulation
Mitral Valve Prolapse
PROBLEMS WITH DERMATAN SULFATE
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FABRICATION OF PDMS CHANNEL
[Nisarga Naik, 33rd Annual International Conference of the IEEE EMBS,IEEE, 2011]
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DIFFERENCE IN ARTIFICIAL LUNG DEVICE
GEOMETRY
Reference
Paper
Geometry
Lung
Device
Made in SolidWorks
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SURFACE MODIFICATION BY SINGLE ION
IRRADIATION
[M. Ionescu et al., Nuclear Instruments and Methods in Physics Research Section B:
Beam Interactions with Materials and Atoms, 20th International Conference on IonBeam Analysis, 2012]
PDMS Layer
Ions
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SIZE DIFFERENCES IN APPLYING TO GAS
DIFFUSION MEMBRANE
PDMS layer in ion irradiation
paperThickness=4m
PDMS gas diffusion layer for
artificial lung deviceThickness =15m
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Use of Dermatan Sulfate in combination with Heparin
to functionalize surface
Dermatan Sulfate has too many complications and is not well
enough understood to be used
Use of fibronectin to endothelialize high aspect ratio
channels
Can endothelialize all the sides of the blood channels
Surface Modification through single ion irradiation
Can increase cell adhesion to PDMS by up to 650%
EVALUATION OF METHODS
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OUR SOLUTION
Blood
PDMS Channel
PDMS Gas Diffusion Membrane
Endothelial
Cells possibly
due to ion
irradiation
Endothelial
Cells due to
fibronectin
Fibronectin
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REFERENCES
D. M. Tollefsen, "Vascular Dermatan Sulfate and Heparin Cofactor II," Progress in Molecular Biology and Translational Science, vol.
93, pp. 351-367, 2010.
N. Naik, V. Kumar, E. L. Chaikof and M. G. Allen, "MEMS-assisted spatially homogeneous endothelialiization of a high length-to-depth
aspect ratio microvascular network," 33rd Annual International Conference of the IEEE EMBS, pp. 290-293, 2011.
M. Ionescu, B. Winton, D. Wexler, R. Siegele, A. Deslantes, E. Stelcer, A. Atanacio and D. Cohen, "Enhanced biocompatibility of PDMS
(polydimethylsiloxane) polymer films by ion irradiation," Nuclear Instruments and Methods in Physics Research B, pp. 161-163,
2011.
W. C. Lamanna, R. Lawrence, S. Sarrazin and J. D. Esko, "Secondary Storage of Dermatan Sulfate in Sanfilippo Disease," The Journal
of Biological Chemistry, 2011.
"What is Dermatan Sulfate?," Conjecture Corporation, 2003-2012. [Online]. Available: http://www.wisegeek.com/what-is-dermatan-
sulfate.htm. [Accessed 8 December 2012].S. Thorslund, J. Sanchez, R. Larsson, F. Nikolajeff and J. Bergquist, "Bioactive heparin immobilized onto microfluidic channels in
poly(dimethylsiloxane) results in hydrophilic surface properties," Colloids and Surfaces, pp. 106-113, 2005.
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Questions
THANK YOU