Biofuge Fresco 100V-120V Green English

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    Biofuge SERVICEMANUAL100/120V Cat. No. 12005522

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    Edition: 01

    17.11.02 MH

    TABLE OF CONTENTS

    Section Title Page

    1 OPERATING INSTRUCTIONS (not part of this manual)

    BROSHURE

    2 SERVICE

    2.1 Servicing Schedule 2-1/2

    2.2 Trouble Shooting 2-3/5

    2.3 Test Points 2-6

    2.4 Mounting and adjustment of the torsion spring 2-7

    2.5 Assembly and adjustment of the drive 2-8

    2.6 Cleaning of Instrument Parts 2-8

    2.7 Electrical Safety Check 2-9

    3 FUNCTIONAL DESCRIPTION3.1 Block Functions 3-1

    3.2 Main Board Functions 3-1/3

    3.3 Key and Indication Board 3-4

    4 DIAGRAMS

    For units with 120V mains voltage

    4.1 Block Diagram 4-1

    4.2 Wiring Diagrams 4-2/3

    4.3 Wiring Connection Diagrams 4-4/6

    For units with 100V mains voltage

    4.4 Block Diagram 4-74.5 Wiring Diagrams 4-8/9

    4.6 Wiring Connection Diagrams 4-10/12

    For units with 100V or 120V mains voltage

    4.7 Main Board, Component Plan, Wiring Diagram 4-13/19

    5 DISASSEMBLY OF INSTRUMENT PARTS

    5.1 Housing / Casing Parts 5-1

    5.2 Sealing Elements 5-2

    5.3 Mechanical Components 5-3

    5.4 Electrical Components 5-4/5

    5.5 Drive Components 5-6

    5.6 Components of Cooling Plant 5-7/8

    6 SPARE PART FIGURES AND LISTS

    6.1 Break down drawings 6-1/3

    6.2 Mounting and adjustment of the torsion spring 6-4

    6.3 Mounting of the temperature sensor 6-5/6

    6.4 Spare Part Lists

    7 CALIBRATION PROCEDURE

    8 TECHNICAL BULLETINS

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    MICROLITRE CENTRIFUGES

    HERAEUS BIOFUGE PICO AND BIOFUGE FRESCO

    MICROLITRE CENTRIFUGES

    HERAEUS BIOFUGE PICO AND BIOFUGE FRESCO

    Compact, fast and quiet

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    The microlitre centrifuges

    in the Biofuge family

    are specially made to handle

    biological separation tasks.

    Whether non-refrigerated

    (Biofuge pico) or

    refrigerated (Biofuge fresco),

    these centrifuges areindispensable in the modern

    biological laboratory.

    Heraeus centrifuges meet

    all current European

    Community guidelines and

    are CE labelled.

    Typical Applications

    q Pelleting DNA and RNA

    q Pelleting of PCR amplified

    nucleic acids

    qAntibody and protein

    precipitates

    q Enzymatic tests

    q Removing cell debris fromcell homogenates

    q Bacterial and yeast cell

    fractionation

    Biofuge pico

    q Incredibly small microlitre

    centrifuge

    q Short braking and

    acceleration times

    q Ergonomic design

    q Max. capacity:

    24 x 1.5/2 ml or

    4 PCR-strips or

    32 individual PCR-tubesq Max. speed: 13,000 rpm

    q Max. RCF: 16,060 x g

    qAerosol tight Easy-Twist-On lid

    Biofuge fresco

    qThe smallest refrigerated

    centrifuge for microlitre tubes

    q Ultra-quiet standstill cooling

    q Powerful CFC-free

    refrigeration unitq Max. capacity:

    24 x 1.5/2 ml or

    4 PCR-strips or

    32 individual PCR-tubes

    q Max. speed: 13,000 rpm

    q Max. RCF: 16,060 x g

    qAerosol tight Easy-Twist-On lid

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    ROTORS AND ACCESSORIES

    FOR BIOFUGE PICO AND FRESCO

    BIOFUGE PICO AND FRESCO

    High performance

    The brushless induction motor

    runs quietly. In 15 seconds

    or less, the rotor accelerates

    to 16,000 x g, which is

    sufficient for all standard

    applications. A quick run key

    is used for short processingruns.

    The high-powered refrigeration

    unit of the Biofuge fresco

    with stand by cooling ensures

    temperatures beneath 0 C

    even at maximum speed.

    Ergonomic design

    The smooth design of the

    casing and the modern

    keypad control are easy-to-

    clean. The straightforward

    arrangement of the keys,

    with icon labels and the

    EASYcontrol microprocessorcontroller, make it simple

    to operate.

    One-touch lid opening allows

    for easy access to the rotor.

    BIOFUGE PICO AND FRESCO

    Compact design

    Create space in your labora-

    tory. Take advantage of the

    23 x 33 cm footprint of the

    small Biofuge pico, which can

    be integrated into any work-

    place. The minimum height of

    the pico casing makes it easierto load the rotor.

    The refrigerated Biofuge fresco,

    with a footprint of 29 x 45 cm,

    is scarcely larger than many

    non-refrigerated centrifuges.

    ROTORS AND ACCESSORIES

    FOR BIOFUGE PICO AND FRESCO

    MIKROLITRE ROTOR INCL. EASY-TWIST-ON AEROSOL TIGHT LID ORDER NO. 75003328

    Biofuge pico Biofuge fresco

    Max. capacity ml 24 x 1.5/2.0 24 x 1.5/2.0

    Max. speed rpm 13,000 13,000

    Max. RCF x g 16,060 16,060

    Acceleration time s 15 15

    Braking time s 12 16

    Max. radius cm 8.5 8.5

    Reducer sleeves Order numbers

    24 reducer sleeves for 0.6/0.5 ml microtubes 76003758

    24 reducer sleeves for 0.4/0.25 ml microtubes 76003759

    24 reducer sleeves for 0.2 ml microtubes 76003750

    PCR-ROTOR INCL. EASY-TWIST-ON AEROSOL TIGHT LID ORDER NO. 75003327

    Biofuge pico Biofuge fresco

    Max. capacity 4 PCR-strips or 4 x 8 PCR-strips or

    32 individual 0.2 ml PCR-tubes 32 individual 0.2 ml PCR-tubes

    Max. speed rpm 13,000 13,000

    Max. RFC x g 12,846 12,846

    Acceleration time s 15 12

    Braking time s 15 16

    Max. radius cm 6.8 6.8

    Optional features include adapter

    sets enabling centrifugation

    of all commercially available micro-

    litre tubes, from 0.6 ml to 0.2 ml

    PCR-tubes.

    With this additional rotor you can

    easily spin smaller sample volumes

    especially 4 PCR-strips(8 tubes each) or 32 individual

    PCR-tubes.

    Both centrifuges are equipped with a 24-place microlitre rotor suitable for 1.5 or 2 ml tubes and optimised

    for applications in the biological sciences. The polypropylene plastic rotor can be autoclaved and offers extensive

    chemical resistance. The Easy-Twist-On aerosol tight lid (included) offers exceptional comfort and safety.

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    Zp

    ico5

    e

    TECHNICAL DATATECHNICAL DATA

    Biofuge pico Biofuge fresco

    Designation Microlitre centrifuge Refrigerated microlitre centrifuge

    Max. RCF 16,060 x g 16,060 x g

    Max. speed 13,000 rpm adjustable in 100 rpm increments 13,000 rpm adjustable in 100 rpm increments

    Min. speed 2,000 rpm 2,000 rpm

    Control Microprocessor-control led v ia EASYcontrol Microprocessor-controlled via EASYcontrol

    Drive Frequency-controlled induction drive Frequency-controlled induction drive

    Acceleration/delay

    Noise level at max. speed < 55 db (A) < 55 db (A)

    standstill cooling < 45 db (A)

    Run time 1 99 min, continuous operation, quick run 1 99 min, continuous operation, quick run

    Program memory The most recently entered data remains in the memory. The most recently entered data remains

    in the memory.

    Construction Galvanised steel chassis with Plastic casing with galvanised steel chassis

    surface-mounted plastic casing and sheet steel lid, stainless steel rotor bowl

    Safety features Lid locks and interlocks Lid locks and interlocks

    Temperature range 9 bis +40 C

    Min. temp. at max. speed 0 C (ambient temp. = 25 )

    Dimensions (HxWxD) 206 x 233 x 335 mm 305 x 290 x 450 mm

    8.1 x 9.2 x 13.2 inch 12.0 x 11.4 x 17.7 inch

    Weight (without rotor) 9.7 kg 27 kg

    21.3 lbs 59.4 lbs

    Power consumption 150 W 270 W

    Standards Manufactured and tested in conformity with EN 61 010-1, EN 61 010-2-020, EN 50 081-1, EN 50 082-1

    Not subject to testing in accordance with UVV VBG 7z

    Refrigerant R 134 a (CFC-free)

    Asia Pacific Kendro Laboratory Products (H.K.) Limited Hong Kong Tel. +852 2711 3910 Fax +852 2711 3858 [email protected]

    Europe, Middle East, Africa

    Kendro Laboratory Products International Sales Hanau Germany Tel. +49 (0) 1805- 536 376 Fax +49 (0) 1805-112 114 [email protected]

    Latin America Kendro Laboratory Products International Sales Newtown, CT USA Tel. +1 203- 270 2080 Fax +1 203- 270 2210 [email protected]

    North America Kendro Laboratory Products Newtown, CT USA Tel. +1 800- 522 7746 Fax +1 203- 270 2166 [email protected]

    Internet http://www.kendro.com

    Quality Products Lifetime Care

    Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards. 2002 Kendro Laboratory Products. All Rights Rese rved.

    Printed in Germany 2C 09/02 4t Druckerei

    For Ordering or Technical Information

    Your Sales Contact

    ORDER NUMBERS 230 V/5060 Hz 110120 V/60 Hz

    Biofuge pico incl. 24-place microlitre rotor 75003328 75003235 75003236

    with Easy-Twist-On aerosol tight lid

    Biofuge fresco incl. 24-place microlitre rotor 75003328 75005521 75005522

    with Easy-Twist-On aerosol tight lid

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    Benchmarks for

    Size and Value.

    Pico and Fresco

    MicrocentrifugesSORVALL

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    Small footprints,BIG value

    Kendro Laboratory Products SORVALL Pico

    and Fresco are the smallest, quietest and quickest

    microcentrifuges going around. Their sleek,

    lightweight designs maximize your available bench

    space, and deliver all the performance you will ever

    need, year in and year out, for a host of separation

    tasks in the microliter sample range.

    Typical applications

    Pelleting DNA and RNA

    Pelleting of PCR-amplified nucleic acids

    Antibody and protein precipitates

    Enzymatic tests

    Removing cell debris from cell homogenates Bacterial and yeast cell fractionation

    SORVALL Pico

    Compact 9" x 13" (233 mm x 335 mm) footprint

    13,000 rpm (16,060 xg)

    Short braking and acceleration times

    High capacity 24 x 1.5/2.0 ml biocontainment rotor NEW PCR strip rotor

    NEW Easy-Twist-On biocontainment lid

    Microprocessor-based keypad control Whisper-quiet, maintenance free brushless drive

    SORVALL Fresco

    The smallest refrigerated centrifuge available

    13,000 rpm (16,060 xg)

    11" x 18" (290 mm x 450 mm) footprint

    High capacity 24 x 1.5/2.0 ml biocontainment rotor NEW PCR strip rotor

    NEW Easy-Twist-On biocontainment lid

    Ultra-quiet standstill cooling Precise temperature control from -9C to 40C

    Whisper-quiet, maintenance free brushless drive

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    Choice of rotors

    Microliter and PCR strip rotors for the SORVALL Pico and Frescoare equipped with biocontainment lids for use with infectious or

    hazardous samples. The aerosol-tight, Easy-Twist-On lid is

    exceptionally easy to use.

    Compact designCreate space in your laboratory. Take advantage of the 233 x 335 mm(9 x 13 in) footprint of the little Pico, which can be integrated

    into any workplace. The low height of the Pico casing makes it

    easier to load the rotor. The refrigerated Fresco, with a footprintof 290 x 450 mm (11 x 18 in), is smaller than many non-refrigerated

    centrifuges.

    Whisper-quietThe Pico and Fresco are valuable aids in the small biological

    laboratory, where noise can be an issue. Their designs take fulladvantage of recent discoveries in aerodynamics, and ensure

    whisper-quiet operation even at maximum speed.

    High performanceThe brushless induction motors of the Pico and Fresco are extremely

    efficient. In 15 seconds or less, they accelerate the microliter rotor toits maximum RCF of 16,000 xg, which is sufficient for all standardapplications. A quick run key is used for short processing runs.

    Stylish, ergonomic designThe smooth design of the casing and the modern keypadcontroller are both easy to clean and pleasing to the eye.

    The straightforward arrangement of the keys, with icon labels

    and the EASYcontrol microprocessor controller, make operationstraightforward. One-touch lid opening allows easy access to the

    rotor chamber.

    Ecological refrigerationThe refrigerated Fresco uses a CFC-free refrigerant.

    The powerful refrigeration unit, with standstill cooling, can maintainconstant temperatures below 0C even at maximum speed.

    Microliter rotor: adapter sets enable

    centrifugation of all commercially

    available microliter and PCR tubes.

    PCR strip rotor: spin 4 PCR strips

    (8 tubes each) or 32 individual PCR tubes.

    Rotors and accessories for the Pico and Fresco

    Microliter rotor 75003328 with Easy-Twist-On biocontainment lid

    Pico FrescoMax. capacity (ml) 24 x 1.5/2.0 24 x 1.5/2.0

    Max. speed (rpm) 13,000 13,000

    Max. RCF (xg) 16,060 16,060

    Acceleration time (s) 15 15

    Braking time (s) 12 16

    Max. radius (mm) 85 85

    PCR strip rotor 75003327 with Easy-Twist-On biocontainment lidPico Fresco

    Max. capacity (4) PCR strips or (4) PCR strips or

    (32) 0.2 ml PCR tubes (32) 0.2 ml PCR tubes

    Max. speed (rpm) 13,000 13,000

    Max. RCF (xg) 12,846 12,846

    Acceleration time (s) 15 12

    Braking time (s) 15 16

    Max. radius (mm) 68 68

    Reducer sleeves Catalog number

    24 reducer sleeves for 0.6/0.5 ml microtubes 76003758

    24 reducer sleeves for 0.4/0.25 ml microtubes 76003759

    24 reducer sleeves for 0.2 ml microtubes 76003750

    Compactmicrocentrifugesfor Life Science

    NEW

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    SORVALL Pico SORVALL Fresco

    Max. RCF (x g) 16,060 16,060

    Max. speed (rpm) 13,000 adjustable in 100 rpm increments 13,000 adjustable in 100 rpm increments

    Min. speed (rpm) 2,000 2,000

    Control Microprocessor-controlled via EASYcontrol Microprocessor-controlled via EASYcontrol

    Drive Frequency-controlled brushless induction drive Frequency-controlled brushless induction drive

    Noise level at max. speed < 55 dB (A) < 55 dB (A)

    standstill cooling < 45 dB (A)Run time 1 - 99 min, continuous operation, quick run 1 - 99 min, continuous operation, quick run

    Program memory The most recently entered data remains in the memory The most recently entered data remains in the memory

    Safety features Lid locks and interlocks Lid locks and interlocks

    Temperature range - -9 to +40C

    Min. temp. at max. speed - 0C (at room temp. = 25C)

    Dimensions (H x W x D) 8.1 x 9.2 x 13.2 in (206 x 233 x 335 mm) 12.0 x 11.4 x 17.7 in (305 x 290 x 450 mm)

    Weight (without rotor) 21.3 lb (9.7 kg) 59.4 lb (27 kg)

    Power consumption 150 W 270 W

    Specifications

    110-120 V/60 Hz 230 V/50-60 Hz

    SORVALL Pico includes 24-place microliter rotor 75003328 75003236 75003235

    with Easy-Twist-On biocontainment lid

    SORVALL Fresco includes 24-place microliter rotor 75003328 75005522 75005521

    with Easy-Twist-On biocontainment lid

    Catalog Numbers

    Australia Kendro Laboratory Products Sydney NSW 2066 Tel. +61 (2) 9936 1540 Fax +61 (2) 9427 9765 [email protected]

    Austria Kendro Laboratory Products GmbH Vienna Tel. +43 (1) 801 40-0 Fax +43 (1) 801 40 40 [email protected]

    Canada Kendro Laboratory Products International Sales Newtown USA Tel. 1 (203) 270-2080 Fax 1 (203) 270-2166 [email protected]

    China Kendro Laboratory Products Beijing Rep. Office Beijing Tel. +86 (10) 6501-3810 Fax +86 (10) 6501-4229 [email protected]

    Kendro Laboratory Products (H.K.) Limited Hong Kong Tel. +852 2711-3910 Fax +852 2711-3858 [email protected]

    Kendro Laboratory Products Shanghai Rep. Office Shanghai Tel. +86 (21) 5490 0216 Fax +86 (21) 5490 0230 [email protected]

    Denmark Axeb AB Albertslund Tel.+45-43 62 46 47 Fax +45-43 62 46 41 [email protected]

    France Kendro Laboratory Products Courtaboeuf cedex Tel.+33 (1) 69 18 77 77 Fax+33 (1) 60 92 00 34 [email protected]

    Germany Kendro Laboratory Products GmbH Hanau Tel +49 (1805) 536 376 Fax +49 (1805) 112 114 [email protected]

    India Kendro Laboratory Products (India) Pvt. Ltd. New Delhi Tel. +91 (11) 618 48 40 Fax +91 (11) 618 53 97 [email protected]

    Italy AHSI S.p.A. Cornate DAdda Tel. +39 (039) 68 27-1 Fax +39 (039) 68 27-500 [email protected]

    Japan Nippon Kendro Co. Ltd. Tokyo Tel. +81 (0) 3 3517-1661 Fax +81 (0) 3 3517-1664

    New Zealand Kendro Instruments New Zealand Auckland Tel. +64 (0) 9-525 03 33 Fax +64 (0) 9-525 03 37 [email protected]

    Poland Kendro Splka z.o.o. Warsaw Tel. +48 (22) 663 43 23 Fax +48 (22) 663 43 25 [email protected]

    Portugal Heraeus S.A. Cacem Tel. +351 (1) 912 08 65 Fax +351 (1) 912 08 60 [email protected]

    Spain Heraeus S.A. Madrid Tel. +34 (91) 358 19 96 Fax +34 (91) 358 20 67 [email protected]

    Sweden Axeb AB Sollentuna Tel. +46 (8) 585 777 50 Fax +46 (8) 623 15 45 [email protected]

    Switzerland Kendro Laboratory Products AG Zurich Tel. +41 (1) 454 12 12 Fax +41 (1) 454 12 99 [email protected]

    Kendro Laboratory Products SA Carouge-Genve Tel. +41 (22) 343 21 67 Fax +41 (22) 342 38 31 [email protected]

    U.K./Ireland Kendro Laboratory Products Ltd. Bishops Stortford Herts Tel. +44 (1279) 827700 Fax +44 (1279) 827750 [email protected]

    USA Kendro Laboratory Products Newtown, CT Tel. +1 (800) 522-7746 Fax +1 (203) 270-2166 [email protected]

    All other countries in

    Asia Pacific Kendro Laboratory Products Newtown, CT USA Tel. +1 (203) 270-2080 Fax +1 (203) 270-2210 [email protected]

    Europe, Middle East, Africa

    Kendro Laboratory Products International Sales Hanau Germany Tel. +49 (1805) 536 376 Fax +49 (1805) 112 114 [email protected]

    Latin America Kendro Laboratory Products International Sales Newtown, CT USA Tel. +1 (203) 270-2080 Fax +1 (203) 270-2210 [email protected]

    Internet http://www.kendro.com

    Quality Products Lifetime Care

    Registered to ISO 9001. Kendro Laboratory Products meet or exceed stringent quality and product safety standards Printed in U.S.A. (11/02) S00239

    2002 Kendro Laboratory Products. All Rights Reserved.

    For Ordering or Technical Information

    SORVALL

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    Maintenance

    Edition: 01 2-1 BIOFUGE fresco17.11.02 MH

    2 Service

    2.1 Servicing Schedule (yearly procedure recommended)2.1.1 Maintenance Routine without Dismantling the Centrifuge2.1.1.1 Electrical Installation and Safety

    switch OFF the centrifuge and disconnect the unit from power, check voltage supply and mainsfusing (15 Amps, slow blow characteristic)

    check condition of plug and wall socket - (let) replace defective parts

    check cord condition and fixing / connection - replace or refit it

    check condition of instrument socket and replace it in case of bad contacts2.1.1.2 Location and Mechanical Installation

    check the base (ground, table, lorry with lockable wheels etc.) for resonance-free and stableconditions

    check for a well ventilated place and sufficient distances to walls or adjacent equipment, withoutexposition to direct sunlight

    check the leveling of the centrifuge drive with use of a spirit level2.1.1.3 Lid Locking Mechanism and Safety Device

    connect the centrifuge to power and switch ON

    check for easy lid closing and self-acting lid opening - if in disorder, readjust lid's swivel hingeand/or torsion spring

    check the central rubber gasket for lid sealing and replace it, if damaged for checking of the safety circuit: start the centrifuge let it shortly run and stop it, the lid must not be

    opened as long as the message "end" will be indicated - if safety circuit is out of function, replacemain board

    2.1.1.4 Cleanliness of Spin Chamber and Motor Casing

    open the lid and remove the rotor (for loosening turn socket wrench in arrow direction - see rim ofrotor chamber, push 2 screw drivers in opposite holes and pull the rotor vertically out)

    clean the spin chamber with a dry and absorbent cloth (remove all dust and moisture - see alsosection for Cleaning)

    check the correct seat and condition of the motor cover and take care of the cleanliness of the

    annular slot around the motor shaft: penetrating fluids can damage the upper motor bearing ,remove fluids with an injector and/or absorbent paper

    2.1.1.5 Rotor and Accessories Condition and Sealing

    check the condition of rotor and accessory parts (especially all supporting or stressed partitions):the rotor and/or accessory parts must not be used any longer, if there are visible traces ofmechanical damage or rust

    check the condition of rotor and/or accessory sealing and replace them in case of malfunction

    2.1.1.6 Rotor and Accessories Condition and Sealing

    check the condition of rotor and accessory parts (especially all supporting or stressed partitions):the rotor and/or accessory parts must not be used any longer, if there are visible traces ofmechanical damage or rust

    check the condition of rotor and/or accessory sealing and replace them in case of malfunction2.1.1.6 Rotor Fixing and Motor Shaft

    check the trouble-free condition of the locking nut and replace it in case of malfunction

    check the condition of the drive motor shaft: the centrifuge must not be used any longer, if the driveshaft is damaged (bend, thread is worn out, etc.)

    2.1.1.7 Temperature Control Circuit

    clean the condenser with a dust exhauster

    check the thermal isolated fixing and the correct place of the temperature sensor

    reinstall the rotor and run the centrifuge at maximum rotor speed and at 4C, check thetemperature 15 minutes later: the displayed and measured value should be approximately thesame (allowed tolerances 2)

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    Maintenance

    Edition: 01 2-2 BIOFUGE fresco17.11.02 MH

    2.1.2 Maintenance Routine after Dismantling the Centrifuge Casing2.1.2.1 Motor Supporting Elements

    check the supporting and damping motor elements and replace them in case of increased rubberabrasion or abundance of imbalance but at least every 3 years

    2.1.2.2 Braking Circuit

    check the function of the braking circuit (warming up of brake resistor, even and noiseless brakeeffect) and replace defective parts in case of malfunction

    2.1.2.3 Ventilation and proper Air Flow

    check the sucking performance of the fans (a piece of paper must be sucked on), if necessaryclean or replace defective fan

    check the air path and lateral sealing: no air must be redrawn from inside, if necessary replace thelateral insulation

    2.1.2.4 Lead and Screwing Connections

    check the terminal and plug connections of all leads and on all boards and electrical components,tighten all loosen screwing connections, refit or replace defective parts

    check the screwing connections of all boards, mechanical and electrical components and re-tighten them if necessary (use screw locking lacquer for motor mounts and lid lock assembly)

    2.1.2.5 Protection Earth Core and Grounding Connections

    check the protection earth core for continuity and all grounding plug connectors

    check isolation resistance and accessible current

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    Maintenance

    Edition: 01 2-3 BIOFUGE fresco17.11.02 MH

    2.2 Trouble Shooting

    Error

    IndicationError Cause

    Possible Error

    SourceCorrective Procedure

    mains fuse or circuitbreaker failed

    check fuse or circuit breaker and replace or switch onagain

    defective mains cord,socket or mains

    switch

    check leads, socket and mains switch, replacedefective parts

    no mainsvoltage supply

    unit fuse or fuses onmain board defective

    Replace the fuse, if fuse blows up again, search forother faults

    faulty connectionfrom main board to

    key board

    check connections on main and key board, checkconnecting leads, replace faulty parts

    no low voltagesupply for

    indication board faulty indication ormain board

    replace main board completely

    displays

    remain

    dark

    interruptedprogram

    NV-RAM out ofsocket or not

    correctly placed

    insert the valid NV-RAM and push it correctly intosocket

    reduced voltagesupply (

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    Maintenance

    Edition: 01 2-4 BIOFUGE fresco17.11.02 MH

    Trouble Shooting

    Error

    IndicationError Cause

    Possible Error

    SourceCorrective Procedure

    Lid was openedmanually during

    run

    Forbidden!Lid opening device

    must only be used atstandstill

    close lid immediately, turn power OFF/ON, wait fortermination of br phase until end message appears

    check leads and connectors to micro switch, in caseof a faulty micro switch, replace lid lock device

    completely

    Defective microswitch or leads or

    connectors to microswitch areinterrupted

    let motor cool down, then check temperature switchand leads with Ohmmeter

    check air cooling, circulation

    LIdmessage

    appears inspeeddisplay

    protection circuit(15V) for lid and

    motortemperaturecontrol wasinterruptedduring run

    Motor over-temperature switch

    has tripped (nosufficient air flow ormotor has run on 2

    phases only)

    check solderless connections XM1-3 and motorwindings, replace defective parts

    micro switch check leads and micro switchOPEn

    message indisplay

    15V supplycircuit is

    interrupted atstandstill

    motor over-temperature switch

    check motor over-temp. switch and leads forcontinuity, replace faulty parts

    short interruption ofmains supply

    Wait for rotor standstill (approx. 75 sec) and re-startbrmessage

    appears inspeeddisplay

    rotor comes tostandstill

    without brakingforce

    bad contact ofinstrument supply

    line

    Check plug contacts of supply line and replacedefective parts if necessary

    rotor is jammedcheck for easy movement, remove any jamming

    objectsrotor didn't turnmotor is jammed remove drive, replace faulty parts

    connection: drivemain board

    check terminal and lead connections, replace faultyparts

    defective drive check resistance of motor windings, replace faultyparts

    E-00flashes

    motor didn't start

    faulty main board replace main board

    faulty temperaturesensor (PT100) or

    leads

    check plug connection and resistance of PT 100,replace faulty sensor

    E-04flashes

    faultytemperaturemeasuringtest: PT100

    substitution byresistors,

    indication afterpower ON

    defective amplifier orconverter

    set the temperature to 20C and watch the display:

    100W (02)C

    120W (502)Cif not replace the main board

    compressor didn't

    work

    check leads and voltage supply on main board and

    compressor, replace defective parts

    chamber over

    temperatureindication value

    >50C loss of refrigerantsearch for leakage, evacuate and refill with newrefrigerant

    E-04flashes

    chamber undertemperature

    indication

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    Maintenance

    Edition: 01 2-5 BIOFUGE fresco17.11.02 MH

    Trouble Shooting

    Error

    IndicationError Cause

    Possible Error

    SourceCorrective Procedure

    E-10flashes

    checksum errorof NV-RAM

    NV-RAM wrong or notinitialised

    insert the correct NV-RAM

    sporadic: strongelectromagnetic

    disturbance

    if error returned very often screen the disturbancesource or operate the centrifuge on another place

    E-11flashes

    error during dataexchange from

    NV-RAM continuously: erroron main board

    replace defective main board

    sporadic: strongelectromagnetic

    disturbance

    if error returned very often screen the disturbancesource or operate the centrifuge on another placeE-23

    flashes

    too muchtolerances during

    temperature

    calibrationcontinuously: error

    on main board replace defective main board

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    Maintenance

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    2.3 Test Points

    Test Points Unit Value Conditions

    mains terminal XN 120V ACall given values are related on 120V (10%) mains voltage for boards

    supply

    terminal XMmotor voltage -

    values must bethe same betweenall 3 motor leads

    (U,V,W)

    115V AC155V AC210V AC

    in each case measured inter 2 motor leads after reaching selected speed -

    no effective values!

    3000min-1 , rotor #3327, #33286000min

    -1

    13000min-1

    acc. time: 15s, dec. time: 17s

    motor current IMcable (U,V,W)

    3 * 0,6A

    soft iron or digital effective measuring meter(true RMS)Max. during acceleration (#3327, #3328)rotor #3327, #3328speed = 13000min

    -1

    320V DC350V DC

    at standstillmax. during braking phase Bremsens (#3327, #3328)

    305V DCrotor #3327, #3328speed = 13000min

    -1

    intermediate

    voltage UDinterU+ (cathode

    D4) and U- (anode

    D5) 275V DC shortly at maximum acceleration (#3327, #3328)

    25mV DC40mV DC65mV DC

    3000min-1

    , Rotor #3327, #33286000min

    -1

    13000min-1

    current ID ofintermediate

    circuit, (measuredas voltage dropacross control

    resistorR100)

    UM = ID * RM

    180mV DC

    -45mV DCshortly at maximum acceleration (#3327, #3328)shortly at maximum braking (#3327, #3328)

    motor windingresistance 20C

    3 x 14W

    -insulation value > 10MW

    switch OFF unit, pull off motor plugs, measure inter 1 -> 2, 2 -> 3, 3 -> 1resistance inter each phase and motor casing

    brake resistorR24 parallel R101

    235W resistor temperature: 20C

    compressor

    terminal XGterminal box

    pull off startingrelay

    -insulation

    3,6W

    5,5W

    10,4W

    0,3W

    > 10MW

    compressor temperature 20Cresistancemain coil (between terminal 13 + 14)auxiliary coil (between terminal 12 + 13)coil starting relayinter phase and compressor casing

    fan motor

    terminal XK150W

    resistancedisconnect one pole of terminal XK

    fan temperature20C

    lid solenoid

    terminal XC20W resistance , not activated

    lid switches

    plug XA15V DC voltage drop by open lid

    Over temperatureswitch Motor

    terminal XB15V DC voltage drop by closed lid and disconnect one pole of terminal XB

    Temp. Sensor

    plug XU 95mV

    voltage drop

    chamber temperature 20C (PT100: 108W)

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    Maintenance

    Edition: 01 2-7 BIOFUGE fresco17.11.02 MH

    2.4 Mounting and adjustment of the torsion spring

    2.4.1 PurposeThe torsion spring offers the user a comfortable support when closing and opening the centrifuge lid.When the "open lid" key is pressed, the lid lock is released on both sides and the lid bursts open andremains in a 20 position. Up to an angle of 45 the lid remains stable in any chosen position, beyond45 it automatically opens to full extent.

    2.4.2 MountingThe torsion spring assembly contains the following parts:

    torsion spring

    2 plain bearing bushes (for shaft guidance)

    2 bushing nipples (for leg catch)

    2 set screws (aid for fixing legs)

    dowel pin (for hooking the torsion spring onto the shaft)

    tube of Loctite 221 (for fastening screws)

    tube of Molykote TP42 (for lubrication of the torsion spring)

    Preparation:The defective torsion spring has been removed as described in Section 5, the old plain bearing bushes

    and bushing nipples have been removed.1. Press the new plain bearing bush from outside into the right-hand bore of the chassis.2. Slip on both bushing nipples up to the head of the hexagon bolt M6x12. Fix the screws again to the

    chassis and secure with Loctite 221.3. Push the old dowel pin carefully from the shaft using a pin punch, then push the new dowel pin on

    using a plane surface (e.g. a wooden panel).

    Mounting the shaft and torsion spring:4. Grease the short end of the shaft evenly using about 5 g of Molykote TP42.

    Attention! Do not cover the plain bearing with grease!5. Slip the torsion spring onto the short end of the shaft (short loop first).6. Push the long end of the shaft through the left-hand bore of the chassis and slip into right-hand

    plain bearing bush.

    7. Press the plain bearing bush via the shaft into the left-hand bore of the chassis.

    2.4.3 AdjustmentPreassembly and alignment of the legs:8. Push both legs onto the ends of the shaft such that the bores are horizontally oriented and the leg

    ends point forward.9. Screw the M8 nuts loosely onto each of the shaft ends to position the legs.

    10. Screw one dowel pin each tightly into the rear threaded bores, thereby fixing them with the bore inthe chassis.

    Stretching the torsion spring:11. Push a suitable screwdriver from behind into the left-hand bore of the shaft and turn totally to the

    front. Grip the shaft via the middle bore with another screwdriver and turn it to approximately 15from horizontal.

    12. Tighten fast both nuts of the shaft.

    2.4.4 Assembling lid and housing13. Put lid in place. It must catch audibly into both locks.14. Screw on front cap screws on both sides, thereby fastening the lid tightly to the two legs.15. Replace one by one the two set screws in the rear bores by the remaining two cap screws.16. Manually release the locks. The lid should come to rest at an angle of 15-30. If this is not the

    case, restretch using the middle bore of the shaft. Should this be impossible, the lid must bedisassembled and the spring must be stretched anew!

    17. If the angle is okay, secure the M8 nuts with one drop of Loctite each on the legs.

    18. Close the lid and replace the rear cap screws on both sides by set screws. Attention! Set screwsmust not jut out of the legs!

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    Maintenance

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    19. Screw lid off and mount housing.20. Reassemble lid as described above and check opening angle of 15-30.

    2.5 Assembly and Adjustment of the Drive2.5.1 Purpose of the adjustmentThe temperature measurement of the Biofuge fresco is done using a slim temperature probe. The tip

    of this temperature probe is fixed at a very short distance to the motor cover as well as to the rotor. Inorder to guarantee an accurate temperature measurement, a defined distance to both rotor and motorcover is mandatory! For this purpose, the drive must be adjusted.

    2.5.2 Mounting / Adjustment of the drive with help of the rotor #3327/3328Fasten rubber buffers to the transverse crosshead using nuts and washers.

    Place transverse crosshead inside the chassis and fasten with screws such that the screws arealigned with the upper ends of the long holes.

    Connect the motor to the rubber buffers using the screws and locking rings.

    Adjusting the transverse crosshead

    Put three cylindrical spacers with a thickness of 6,00,1 mm (diameter 10 to 20mm) for rotor#3327/3328 into the evaporator.

    Position the rotor onto the motor shaft and fasten with flanged nut.

    Loosen the screws about turn and carefully move transverse crosshead downward until the rotorrests on top of the spacers. Attention! Make sure the transverse crosshead is horizontally placed.Besides, the rubber buffers must not be stretched or tilted.

    Fasten all 8 screws of the transverse crosshead.

    Checking the adjustment

    Replace the spacers by three 5,00,1 mm thick spacers (diameter 10 to 20mm) for rotor#3327/3328. Fasten rotor again as prescribed.

    Turn rotor manually. The motor is correctly mounted if the rotor does not touch the spacers. If itdoes, the adjustment must be repeated.

    Remove spacers and secure screws with a drop of screw lock.

    Reinstall centrifuge and rotor as prescribed and do a test run.

    2.6 Cleaning of Instrument Parts

    ATTENTION - WARNING!

    The electrical and electronic components must not be cleaned with moist detergents!For Cleaning the centrifuge housing or its accessories see Operating Instructions section 1(maintenance and care).

    Electronic components

    Clean dusty components carefully with a dry and soft brush and remove loose dust with avacuum cleaner.

    FansScratch off carefully with a knife or similar tool the crusted dirt from the fans blades. Resultinggrooves or marks must be removed subsequently with a fine abrasive cloth. Loose dirt is toremove with an absorbent cloth or vacuum cleaner.

    Vent holesRemove dirt from the vent grid behind the front panel by using a brush and vacuum cleaner.

    Condensers of cooling plantThe louvers between the condensers heat air deflectors have to be vacuum cleaned. In case ofpersistent dirt the unit must be carried to an appropriate place where the condensers can becleaned with compressed air from inside to outside. Bent heat air deflectors have to be realignedcorrectly.

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    Maintenance

    Edition: 01 2-9 BIOFUGE fresco17.11.02 MH

    2.7 Electrical Safety Check

    ATTENTION!

    A final electrical safety check must be performed after each maintenance and/or repair!

    Resistance check of protective conductorThe measuring value of the resistance between the mains plug's grounding pin and the grounding

    conductors of the motor, electronic chassis and the casing must not exceed 200 mW.

    Insulation resistance CheckCheck also the insulation resistance between the poles of the mains plug and the grounding

    conductor; the resistance value must be more than 2 MW.

    Accessible current measured to EN 61 010The accessible current must not exceed 3.5 mAmps in single fault condition (interruptedprotection earth wire)! In accordance with the EN61010, IEC1010 and UL3101 such a faultcondition can be reproduced by the following measuring circuit.

    500W1%

    L (N)

    N (L)

    PE

    10kW5%

    0,022F5%

    VAC

    0,22F5%

    Steckergehuse / plug-in casing

    Spezifaktionen fr Megert- TRMS, DC - 5kHz oder mehr- Eingangswiderstand > 1M- Toleranz 5% oder besser- Crest Faktor 5 oder besser

    W

    Specifaction for the meter- TRMS, DC - 5kHz or more

    - Input resistance > 1M- Tolerance 5% or better- Crest Factor 5 or better

    W

    Krperstrom:accessible current: I [mA] = U [mV] / 500:

    U = 1750mV I = 3,5mAmax max

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    FUNCTIONAL DESCRIPTION

    Edition:01 3-1 Biofuge fresco28.11.02 MH

    3 Functional Description

    3.1 Block Functions

    The BIOFUGE fresco is a microprocessor controlled laboratory tabletop centrifuge with inductiondrive motor and refrigerator. Following boards and components are located inside of the unit (seeblock diagram section 4 page 4-1):

    Two poles mains switch is mounted in the bottom of the front panel's right side

    Main board with microprocessor part and power electronics, 2 fuses (4AT) are serving for boardprotection

    Key and indication board (EASYCONTROL programming with temperature display)

    Three phase induction drive motor with an integrated thermal over temperature switch (cut-out at140C)

    Temperature sensor PT100

    Compressor with starting relay (inside terminal box) and starting capacitor

    Two lid lock assemblies with solenoid and integrated micro switches (mechanical bolt keeper,magnetically dislocking)

    Brake resistor mounted on main board

    Two fans (parallel connected) for cooling of condenser, drive, compressor and electronics

    3.2 Main Board Functions

    The main board is mounted on the rear side of the front panel. The components on main board arearranged in following groups (see also wiring diagram page 4-2 and 4-3).

    Board fusing (2 x 4 Amps. slow blow feature)

    Noise Filter for suppression of radio interference, caused by the fast switching power electronics(9,9 kHz), in accordance with the German Regulation VDE 871, grade B

    Power pack for low voltage supply of microprocessor part (potential separated by transformer) andpower electronics (mains potential)

    Triac control circuit for both parallel connected fans

    Triac control circuit for both parallel connected lid solenoids ( DC supply via bridge rectifier)

    Triac control circuit for compressor relay

    Bridge rectifier for DC intermediate circuit supplying brake control path and frequency converter

    Microprocessor part with Controller (central processing unit) and ASIC80 (specific integrated circuitfor controlling of centrifuge functions)

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    FUNCTIONAL DESCRIPTION

    Edition:01 3-2 Biofuge fresco28.11.02 MH

    3.2.1 Power Pack

    The power pack consists of a transformer, bridge rectifier and voltage regulator.

    U1 = 5 V supplies central processor, key and indication board and temperature measuringcircuitreference potential A1: connected to protective conductor (GND)

    Additional generated by one way rectifying from the mains voltage: U2 = 15 V supplies control electronics of frequency converter via the protection circuit of FET

    over temperature switch (118C - normal closed), closed lid switch and motor overtemperature switch (normal closed)reference potential:

    A2! mains potential! EXERCISE CAUTION when measuring etc.

    3.2.2 Intermediate Circuit with Brake Path and Frequency ConverterIntermediate CircuitThe DC intermediate circuit serves as an energy store between the AC power input and thetransmitted motor performance. The intermediate circuit consists of a bridge rectifier (4 diodes) andone reservoir capacitor which is softly charged, continuously supplied during operation and slowlydischarged after power off.

    Brake PathElectrical power is fed back into the intermediate circuit during motor deceleration (motor acts asgenerator). This braking power is transformed into heat by two big brake resistors (parallel connected)on board so that the intermediate circuit voltage does not rise to an excessive level (see section 2 -test points on main board). The brake resistors are switched into the intermediate circuit by a fastswitching transistor (pulse width modulation). This transistor is voltage dependant controlled by a self-acting stage (closed loop).

    Frequency ConverterThe motor is 3-phased provided with chopped direct voltage blocks. These blocks are variable infrequency and pulse width modulation and are dephased to 120 between the motor windings.Beginning with low frequency and small pulse width chopped blocks, both parameters will beincreased up to the operators set speed. By this variation the acceleration power of the drive motorcan be effected. For deceleration the frequency and pulse width parameters will be reduced. Thefrequency converter is protected against over current, over temperature and over voltage.

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    FUNCTIONAL DESCRIPTION

    Edition:01 3-3 Biofuge fresco28.11.02 MH

    3.2.3 Microcontroller (Central Processing Unit)The controller block includes the central processor unit (CPU) 80C21 PLCC and the data storage (NV-RAM) 9346 with the software identification No's. for:

    CPU: 097 8 xx (first displayed No. in speed, time and temperature field)

    RAM: 415 8 xx (second displayed No. in speed, time and temperature field)

    The second parts of these No.'s (xx displayed in the temperature field) will be upgraded if necessary.The ROM with control program is integrated in the CPU. The most important operating parameters(e.g. the maximum data or the last operator settings) are stored in the non volatile (NV) -RAM.

    The CPU co-operates with the ASIC 80 component via the 8 bit data bus and some control lines. TheASIC 80 is a specially designed integrated circuit for the driving of our centrifuges (with inductionmotors), containing additional functions for temperature measuring and electronics protection.

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    FUNCTIONAL DESCRIPTION

    Edition:01 3-4 Biofuge fresco28.11.02 MH

    3.3 Key and Indication BoardThe key and indication board is mounted behind the display. The connection to the main board is doneby a 14 pole flat cable. The 7-segment displays, the control key LED and the operating keys are alsomanaged by the CPU. For the programming serves EASYCONTROL (see Operating InstructionManual).

    Function of key elements

    keys name function(s)

    S1 lid symbol to open the lid (only possible at standstill)

    S2 double arrow quick run, to start - keep the key pressed

    S3 square symbol stop, to terminate the centrifugal run manually

    S4 arrow to right start, to set a centrifugal run in rotation

    S5 speed arrow down to decrease the value of the set speed

    S6 speed arrow up to increase the value of the set speed

    S7 temp. arrow down to decrease the value of the set temperature

    S8 temp arrow up to increase the value of the set temperature

    S9 time arrow down to decrease the value of the set time

    S10 time arrow up to increase the value of the set time

    Function of indicating elements

    indicat. name function(s)

    H01,H02temperature

    rangeindication of sample temperature, right part of softwareidentification numbers of CPU and NV-RAM

    H03,H04 time rangeindication of centrifugation time and hold (hd),left part of software identification numbers of CPU and NV-RAM

    H5 H7 speed range

    indication of speed [1000 (min-1

    )]

    left part of software identification number

    run finished (End) error messages like (LId, E- )

    H8, H9,H12, H13

    rotation-LEDs indication of rotation after starting till End

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    SCHEMATIC DIAGRAMSFor units with 120V mains voltage

    4 - 1 BIOFUGE fresco

    Block Diagram

    16.12.02 He

    Edition: 01

    Y1 Y2

    key and indication board

    motor

    M1

    3~

    brake resistor

    lid solenoid

    lid lock left side lid lock right side

    lid solenoidlid switch lid switch

    F3

    overtemp. switch

    XA XBXC XM XU

    instrument plug

    main board

    M2

    XGXK

    XN

    fan for compressor, condenser

    M3 M4

    V1PT100

    compressortemp. sensor

    mains switch

    legend

    screwing connection plug connection

    XF

    XF

    S1

    CPUNV-RAM

    x 1 0 0 01 0 0 0

    BIOFUGE

    S1S3 S2S4

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    4 - 2 BIOFUGE fresco16.12.02 He

    Edition: 01 Wiring Diagram

    SCHEMATIC DIAGRAMSFor units with 120V mains voltage

    7805

    transformer, rectifiers and regulators

    F1

    XA/1

    lid switch

    S1..S4

    main board

    M2

    5V (VCC)

    XV/1 XV/2

    8AT

    XA/2XB/1 XB/2 XC/1 XC/2

    J

    J

    Y1

    Y2

    PTC

    NTC

    autom. brake control

    +15V

    DC intermediate circuit0V=A2

    A1=GND

    driving of FETs (frequency converter)

    lid solenoid

    CS

    fan for condenser

    noise filter

    compressor with starting relay

    XG/1

    K1

    "E-07"

    "E-17"

    "E-17"

    "E-08"

    "E-07"

    "E-07"

    "E-07" "LId"

    14 10

    11

    13

    M3

    M2

    XL

    XN

    XPE

    3

    4

    PE chassisPE motor

    PE compressorPE condensor fan

    1

    5

    2

    R1 220brake resistor

    "E-08"

    instrument plugwith mains switch

    S0

    XG/2

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    4 - 3 BIOFUGE fresco

    Wiring Diagram

    16.12.02 He

    Edition: 01

    SCHEMATIC DIAGRAMSFor units with 120V mains voltage

    K1(M2)5

    U

    F

    temperature sensorPT100

    JV1

    "E-7"

    1

    2

    XM/1 XM/2 XM/3

    F3

    M1

    overtemperature switch

    Motormotor

    3

    4

    U1 (5V)A1 = GND

    U1 (5V)A1

    U2 (15V)

    A2 R44

    frequency converter

    P part

    key and indication part

    EASYCONTROL

    main baord part 2

    ASIC80

    dyndyn

    dyn

    CPU NV-RAM

    physical separation

    INHIBIT

    RESET

    2x6 HGTPs

    Y1, Y2

    E- 8

    "E- 0"

    "E- 0"

    E- 0

    "E-10""E-11"

    "LId"

    ii

    i

    15V LId...

    "LId"

    "OP"

    13 8 4

    3

    XF

    H1

    H6H8

    H9H7

    H2H3 H4 H5

    XF14

    3

    S1S2

    S4 S3 S8 S7

    S6S5

    A,BF,E

    8

    A,B,C,DE,F,G,DP

    UD-*

    UD+

    current control res.

    MUX

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    4 - 4 BIOFUGE fresco16.12.02 He

    Edition: 01 Wiring Connections Diagram

    SCHEMATIC DIAGRAMSFor units with 120V mains voltage

    S 1

    S 2

    toroidial core, Valvo4322 020 97183+ cable tie perform a most narrow winding

    as possible!

    no. 1 no. 2 no. 3

    key and indication board

    XA

    F1

    XM3XM2XM1

    XC

    PE

    XU

    XK

    XG

    XN

    drive motor

    M 3M 2

    Y 1

    Y 2

    instrument plug

    12

    45

    I 0

    heat sink

    lid switchesS1 u. S2

    lid solenoidsY1 und Y2

    fans for condenserM2 und M3

    M4compressor

    mains switchS 0

    M 1

    brake resistorR 101

    XB1 XB2

    motor overtemp. switchXB1 u. XB2

    temperature sensor

    Y 1

    Y 2

    S1

    S2

    1

    4

    2

    14

    2

    lid lock, right side

    lid lock, left side

    1

    1

    4

    4

    a

    a

    b

    b

    a

    a

    b

    b

    PE

    N L

    L

    N

    PE - motor

    PE - compressor

    bottom platebottom plate

    PE - fan M2 PE - fan M3

    mounting mains ground

    amored chamber

    pass through from inside all leadsmarked by ellipse

    lead-in hole for sensor

    1

    2

    1

    2

    1

    2

    mounting of unit ground, right side in front mounting of unit ground, left side in front

    PE - armored chamber foot, right side in the rear

    PE - main board

    1x PE - terminal 1 pole1x screw M4x10mm2x fan washer 4,3mm

    1x screw M4x25mm1x PE - terminal 3 pole6x fan washer 4,3mm3x nuts M4

    1x PE - terminal 3 pole1x screw M4x10mm2x fan washer 4,3mm

    1x screw M4x16mm2x fan washer 4,3mm1x nut M4

    PE - mains groundPE - armored housing

    PE - motor(from motor harness)

    main board

    S3

    S4

    4

    4

    2

    2

    1

    1

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    4 - 5 BIOFUGE fresco16.12.02 He

    Edition: 01 Wiring Connections Diagram

    SCHEMATIC DIAGRAMSFor units with 120V mains voltage

    compressor terminal box

    startingcapacitor

    starting relay

    10 11

    12 13

    14 PE terminal box

    PE

    + +

    starting capacitorterminal: 11 u. 13

    mains - L

    mains - N

    fans

    drive motor wiring

    XB1

    XB2

    XM1

    XM2

    XM3

    overtemp. switch

    motor connections

    bottom plate

    XK/L

    XK/N

    wiring fans

    M2 M3PE - LfterPE - fans

    5x 400 mm

    PE - main boardunit ground, left side in front

    PE - motor, 300mmto amored chamber in front

    XG/L

    XG/N

    compressor wiring

    PE - compressor to mains ground

    shrinkage hose 12,7mm+ 1 cable tie SST1

    120mm

    1x PE - terminal 1 pole1x screw M4x10mm2x fan washer 4,3mm1x PE - terminal 1 pole

    1x screw M4x10mm2x fan washer 4,3mm

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    4 - 6 BIOFUGE fresco

    Wiring Connections Diagram

    16.12.02 He

    Edition: 01

    SCHEMATIC DIAGRAMSFor units with 120V mains voltage

    switch

    coil

    temperature sensor

    overtemp.switch

    drive

    overtemp. switch

    lid lock, switches lid lock, coilfans compressor mains input

    fan M3fan M2

    mains switch

    S0

    unit socket

    adhesiveclip

    PE - fan 2 PE-fan 3

    fan2,

    leftside

    fan3,rightside

    frontofunit

    clip6,5mm

    clip6,5mm

    *

    *

    *

    KM

    free cables lengthup to first fixing clip

    plug length from clipdesignation

    XA

    XB1/2

    XM1/3

    XC

    XG

    XK

    lid switches S1 + S2

    overtemperatur switch

    motor, connect. 1, 2, 3

    lid solenoids Y1 + Y2

    compressor

    fans M2 + M3

    125 mm

    140 mm

    140 mm

    150 mm

    300 mm

    210 mm

    cable conduction unit

    detail

    view

    offans

    cablecon

    ductionbyclips

    drive

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    SCHEMATIC DIAGRAMSFor units with 100V mains voltage

    4 - 7 BIOFUGE fresco

    Block Diagram

    16.12.02 He

    Edition: 01

    Y1 Y2

    key and indication board

    motor

    M1

    3~

    brake resistor

    lid solenoid

    lid lock left side lid lock right side

    lid solenoid

    auto transformer100/115V

    lid switch lid switch

    F3

    overtemp. switch

    XA XBXC XM XU

    instrument plug

    main board

    M2

    XGXK

    XN115V

    100V

    fan for compressor, condenser

    M3 M4

    V1PT100

    compressortemp. sensor

    mains switch

    legend

    screwing connection plug connection

    XF

    XF

    S1

    CPUNV-RAM

    x 1 0 0 01 0 0 0

    BIOFUGE

    S1S3 S2S4

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    Edition: 01 Wiring Diagram

    SCHEMATIC DIAGRAMSFor units with 100V mains voltage

    7805

    transformer, rectifiers and regulators

    F1

    XA/1

    lid switch

    S1..S4

    main board

    M2

    5V (VCC)

    XV/1 XV/2

    8AT

    XA/2XB/1 XB/2 XC/1 XC/2

    J

    J

    Y1

    Y2

    PTC

    NTC

    autom. brake control

    +15V

    DC intermediate circuit0V=A2

    A1=GND

    driving of FETs (frequency converter)

    lid solenoid

    CS

    fan for condenser

    noise filter

    compressor with starting relay

    XG/1

    K1

    "E-07"

    "E-17"

    "E-17"

    "E-08"

    "E-07"

    "E-07"

    "E-07" "LId"

    14 10

    11

    13

    M3

    M2

    XL

    T0 XN

    XPE

    3

    4

    PE chassisPE motor

    PE compressorPE condensor fan

    1

    5

    2

    R1 220brake resistor

    "E-08"

    instrument plugwith mains switch

    auto transformer100/115V

    S0

    XG/2

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    Edition: 01 Wiring Diagram

    SCHEMATIC DIAGRAMSFor units with 100V mains voltage

    K1(M2)5

    U

    F

    temperature sensorPT100

    JV1

    "E-7"

    1

    2

    XM/1 XM/2 XM/3

    F3

    M1

    overtemperature switch

    Motormotor

    3

    4

    U1 (5V)A1 = GND

    U1 (5V)A1

    U2 (15V)

    A2 R44

    frequency converter

    P part

    key and indication part

    EASYCONTROL

    main baord part 2

    ASIC80

    dyndyn

    dyn

    CPU NV-RAM

    physical separation

    INHIBIT

    RESET

    2x6 HGTPs

    Y1, Y2

    E- 8

    "E- 0"

    "E- 0"

    E- 0

    "E-10""E-11"

    "LId"

    ii

    i

    15V LId...

    "LId"

    "OP"

    13 8 4

    3

    XF

    H1

    H6H8

    H9H7

    H2H3 H4 H5

    XF14

    3

    S1S2

    S4 S3 S8 S7

    S6S5

    A,BF,E

    8

    A,B,C,DE,F,G,DP

    UD-*

    UD+

    current control res.

    MUX

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    Edition: 01 Wiring Connections Diagram

    SCHEMATIC DIAGRAMSFor units with 100V mains voltage

    S 1

    S 2

    toroidial core, Valvo4322 020 97183+ cable tie

    S 1

    S 2

    toroidial core, Valvo4322 020 97183+ cable tie perform a most narrow winding

    as possible!

    no. 1 no. 2 no. 3

    key and indication board

    XA

    F1

    XM3XM2XM1

    XC

    PE

    XU

    XK

    XG

    XN

    drive motor

    M 3M 2

    Y 1

    Y 2

    instrument plug

    12

    45

    100V

    115V

    black

    blue

    redred

    I 0

    heat sink

    lid switchesS1 u. S2

    lid solenoidsY1 und Y2

    fans for condenserM2 und M3

    M4compressor

    auto transformerT0

    mains switchS 0

    M 1

    brake resistorR 101

    XB1 XB2

    motor overtemp. switchXB1 u. XB2

    temperature sensor

    Y 1

    Y 2

    S1

    S2

    1

    4

    2

    14

    2

    lid lock, right side

    lid lock, left side

    1

    1

    4

    4

    a

    a

    b

    b

    a

    a

    b

    b

    PE

    N L

    L

    N

    PE - motor

    PE - compressor

    bottom platebottom plate

    PE - fan M2 PE - fan M3

    mounting mains ground

    amored chamber

    pass through from inside all leadsmarked by ellipse

    lead-in hole for sensor

    1

    2

    1

    2

    1

    2

    mounting of unit ground, right side in front mounting of unit ground, left side in front

    PE - armored chamber foot, right side in the rear

    PE - main board

    1x PE - terminal 1 pole1x screw M4x10mm2x fan washer 4,3mm

    1x screw M4x25mm1x PE - terminal 3 pole6x fan washer 4,3mm3x nuts M4

    1x PE - terminal 3 pole1x screw M4x10mm2x fan washer 4,3mm

    1x screw M4x16mm2x fan washer 4,3mm1x nut M4

    PE - mains groundPE - armored housing

    PE - motor(from motor harness)

    main board

    S3

    S4

    4

    4

    2

    2

    1

    1

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    Edition: 01 Wiring Connections Diagram

    SCHEMATIC DIAGRAMSFor units with 100V mains voltage

    compressor terminal box

    startingcapacitor

    starting relay

    10 11

    12 13

    14 PE terminal box

    PE

    + +

    starting capacitorterminal: 11 u. 13

    mains - L

    mains - N

    fans

    drive motor wiring

    XB1

    XB2

    XM1

    XM2

    XM3

    overtemp. switch

    motor connections

    bottom plate

    XK/L

    XK/N

    wiring fans

    M2 M3PE - LfterPE - fans

    5x 400 mm

    PE - main boardunit ground, left side in front

    PE - motor, 300mmto amored chamber in front

    XG/L

    XG/N

    compressor wiring

    PE - compressor to mains ground

    shrinkage hose 12,7mm+ 1 cable tie SST1

    120mm

    1x PE - terminal 1 pole1x screw M4x10mm2x fan washer 4,3mm1x PE - terminal 1 pole

    1x screw M4x10mm2x fan washer 4,3mm

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    Edition: 01 Wiring Connections Diagram

    SCHEMATIC DIAGRAMSFor units with 100V mains voltage

    switch

    coil

    temperature sensor

    overtemp.switch

    drive

    overtemp. switch

    lid lock, switches lid lock, coilfans compressor mains input

    fan M3fan M2

    mains switch

    S0

    unit socket

    adhesiveclip

    PE - fan 2 PE-fan 3autotransformer

    100/115V

    auto transformerBV30326

    Prim.100V Sek.115V

    fan2,

    leftside

    fan3,rightside

    frontofunit

    clip6,5mm

    clip6,5mm

    *

    *

    *

    KM

    free cables lengthup to first fixing clip

    plug length from clipdesignation

    XA

    XB1/2

    XM1/3

    XC

    XG

    XK

    lid switches S1 + S2

    overtemperatur switch

    motor, connect. 1, 2, 3

    lid solenoids Y1 + Y2

    compressor

    fans M2 + M3

    125 mm

    140 mm

    140 mm

    150 mm

    300 mm

    210 mm

    cable conduction unit

    detail

    view

    offans

    cablecon

    ductionbyclips

    drive

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    Component Plan Main Board

    16.12.02 He

    Edition: 01

    SCHEMATIC DIAGRAMS

    XDAnschlussServiceplugservice

    XUSteckerTemperaturfhler

    plugtemperature sensor

    XM1-XM3AnschlussMotorterminaldrive motor

    XASteckerDeckelschalterpluglid lock, switches

    XB1, Xb2AnschlussMotorbertemp.terminalovertemp. switch

    XNAnschlussNetzterminalmains

    XGAnschlussKhlmaschineterminalcompressor

    XKAnschlussLfterterminalfans

    XCAnschlussDeckelspuleterminallid solenoid

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    Edition: 01 Wiring Diagram Main Board

    SCHEMATIC DIAGRAMS

    Controller

    page 4-16

    PHASE1_U

    PHASE2_U

    PHASE3_UPHASE3_O

    PHASE2_O

    PHASE1_O

    DECKELAUF

    /UEBERSPANNUNG

    TEMPERATUR

    TEMP_0

    TEMP_1

    INHIBIT

    DECKELSCHLOSS

    KUEHLUNG

    UEBERSTROM

    LUEFTER

    power electronic

    page 4-19

    +15

    U+

    PHASE1_U

    PHASE3_OPHASE3_U

    PHASE2_UPHASE2_O

    PHASE1_OINHIBIT

    U-*

    protection circuit

    page 4-17

    +15

    U+

    ZK2

    U0

    U-

    DECKELAUF

    /UEBERSPANNUNG

    UEBERSTROM

    U-*

    temperature measurement

    page 4-15

    TEMPERATUR

    TEMP_0

    TEMP_1

    power supply

    page 4-15

    U-

    U0

    ZK2

    U+

    DECKELSCHLOSS

    KUEHLUNG

    LUEFTER

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    Edition: 01 Wiring Diagram Main Board

    SCHEMATIC DIAGRAMS

    ZK2

    R18

    470RSMD

    D9

    1N4007

    Hilfszwischenkreis

    R162R5NTC

    C162n2

    Y

    EMI FilterFunkentstoerfilter

    1

    2 4

    3L1

    2x5.6mH2A

    Power Connection

    L1

    2

    XN F1

    SI 8AT6.3x32 UL

    PE

    N

    XPE1

    R17120k

    C150u6X

    D4

    P600K

    R1456k

    C13470u200VC19

    220n400V

    1 2BR1

    BR_POWER

    U+

    U0

    DC intermediate circuit

    U-

    R1556k

    C14470u200V

    C92n2Y

    C102n2Y

    D5

    P600K

    120V

    T1

    T120V/9VBV28969 GASS

    V7SM4007SMD

    V5SM4007SMD

    V6

    SM4007SMD

    C201000u25V

    C2110u

    VI2 GND

    1

    VO 3

    V1780M05C

    not placed

    VCC

    C7110u

    SMD 25V TA

    C12100nSMD

    GND

    R21

    330RSMD

    KUEHLUNG

    C11100nSMD

    V8

    SM4007 SMD

    1

    2

    Z10u1/100R

    1

    2

    4

    3K1a

    REL120V HL1-HP-AC120

    compressorXG

    1

    2

    lid solenoidXC

    1

    2

    V50

    SM4007SMD

    V61SM4007SMD

    V62SM4007SMD

    R28100R

    SMD

    C172n2

    Y

    R1947R

    SMD

    V2

    T1213

    R201kSMD

    6 1

    24

    D6S21ME4

    6 1

    24

    D7S21ME4

    GND

    to drive lid lock

    to drive fans

    to drive compressor

    DECKELSCHLOSS

    LUEFTER

    R27

    330RSMD

    GND

    R2247R

    SMD

    V3

    T1213

    R231kSMD

    6 1

    24

    D8S21ME4

    1

    2

    Z20u1/100R

    R462R6 PTC

    V63

    SM4007SMD

    fansXK

    1

    2

    R29100R

    SMD

    C182n2

    Y

    R2547R

    SMD

    V4

    T1213

    R261kSMD

    GND

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    Edition: 01 Wiring Diagram Main Board

    SCHEMATIC DIAGRAMS

    C2100nSMD

    R222kSMD

    auto calibration

    R1

    1kSMD

    3

    21

    8

    4

    D1ATLC272SMD

    C5

    1nSMD

    VCC

    R4270kSMD VCC VDD

    (74HCT4052)

    VEEC3

    100nSMD

    C4100nSMD

    VSS GND

    Voltage to Frequency Converter

    R5100KSMD

    R322kSMD 5

    67

    D1BTLC272SMD

    X012

    X114

    X215

    X311

    Y01

    Y15

    Y22

    Y34

    INH6

    A10

    B9

    X 13

    Y3

    D274HC4052SMDR12

    120RSMD 0.1%

    R13100R

    SMD 0.1%

    GND

    1 1

    2 2

    temperature sensorPt100XU1

    R9390RSMD

    TEMP_1TEMP_0

    GND

    Temp_1 Temp_0 X,YA B0 0 100R1 0 120R1 1 PT100

    GND

    COMP IN7

    CURR IN1

    THRES6

    VS

    8

    R/C 5

    F OUT 3GND

    4

    REF

    CUR

    2

    D3LM331

    C61 u S MD 35V Ta

    R1010kSMD

    R1139kSMD

    C810u

    VCC

    GND GND

    C7310uSMD 25V TA

    nicht bestuecktnot placed

    temperature signal

    C1220n

    Fo

    TEMPERATUR

    R712kSMD

    R6100KSMD

    C71 u S MD 35V Ta

    GND

    R82k7SMD

    GND

    GND

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    Edition: 01 Wiring Diagram Main Board

    SCHEMATIC DIAGRAMS

    C30100nSMD

    C31100nSMD

    VCC VDD

    not placed

    C23 22p SMD

    C24 22p SMD

    J1J 11.14112

    GND

    GND

    GNDGND

    R47100RSMD

    98

    D15D74HC14SMD EA/VP35

    X121

    X220

    RESET10

    INT014

    INT115

    T016

    T117

    P1.02

    P1.13 P1.24

    P1.35

    P1.46

    P1.57

    P1.68

    P1.79

    P0.0 43

    P0.1 42

    P0.2 41

    P0.3 40

    P0.4 39

    P0.5 38

    P0.6 37

    P0.7 36

    P2.0 24

    P2.1 25

    P2.2 26

    P2.3 27

    P2.4 28

    P2.5 29

    P2.6 30

    P2.7 31

    RD 19

    WR 18

    PSEN 32

    ALE/P 33

    TXD 13

    RXD 11

    D13

    80C21PLCC PROG 0978

    AD0AD1AD2AD3AD4AD5AD6AD7

    AD0AD1AD2AD3AD4AD5AD6AD7

    WR_6X

    GND 1312

    98

    21

    1110

    GND

    ABCDEFG

    DP

    A B C D E F GDP A B C D E F GDP A B C D E F G DP A B C D E F G DP

    E

    F

    H12 LED

    RT 3MMH13 LED

    GN 3MM

    V10BC807

    R311k

    SMD

    V18BC807

    R521k

    SMD

    V19BC807

    R531k

    SMD

    V9BC807

    R321k

    SMD

    V17BC807

    R511k

    SMD

    C32

    100nSMD

    A1

    B2

    C3

    G16

    G2A4

    G2B5

    Y0 15

    Y1 14

    Y2 13

    Y3 12

    Y4 11

    Y5 10

    Y6 9

    Y7 7

    VCC

    16

    GND8D14

    74HCT138SMD

    C37 10u

    11223344556677

    8

    8

    9

    9

    10

    10

    11

    11

    12

    12

    13

    13

    14

    14

    XfDIP14

    13

    12111098

    12

    VCC

    11

    22

    33 44

    55

    66

    77 8 89 9

    10 1011 1112 12

    13 1314 14

    XF

    DIP14

    >>>

    >>

    CS 1

    SK 2

    DI 3

    DO 4GND5

    VCC8

    NC6

    NC7

    D11

    9346

    /DECKEL

    /DECKELAUF

    /LUEFTERVCC

    56

    D15C

    74HC14SMD

    GND

    34D15B

    74HC14SMD

    R42

    10k SMD

    R41

    10k SMD

    VCC

    1234

    XE

    Burndy 4pol

    11 10D15E

    74HC14 SMD

    13 12

    D15F

    74HC14 SMD

    LUEFTER

    KUEHLUNG

    VCC

    GND

    PC

    GND

    1234

    XD

    Burndy 4pol

    S1 = LIDS2 = Quick RunS3 = STOPS4 = STARTS5 = - SPEEDS6 = + SPEEDS7 = - TEMPS8 = +TEMPS9 = - TIMES10= + TIME

    V11

    S2S1 S3 S4

    V12 V13 V14

    S5 S6 S7

    V15 V16 V20 V53 V54

    S9S8

    V55 V56 V57

    S10R304k7SMD

    7 6 4 2 1 910 5 3

    H7HDSP-5551

    7 6 4 2 1 910 5 3

    H6HDSP-5551

    7 6 4 2 1 910 5 3

    H5HDSP-5551

    A

    B

    H8 LED

    GE 3MMH9 LED

    GN 3MM

    Time

    7 6 4 2 1 910 5 3

    H3HDSP-5551

    7 6 4 2 1 910 5 3

    H4HDSP-5551

    Temp

    7 6 4 2 1 910 5 3

    H2HDSP-5551

    8x220R SMD

    7 6 4 2 1 910 5 3

    H1HDSP-5551

    D12 Q1 19

    D23 Q2 18

    D34 Q3 17

    D45 Q4 16

    D56 Q5 15

    D67 Q6 14

    D78 Q7 13

    D89 Q8 12

    C11

    OC1

    D16

    74HC573 SMD

    R5010kSMD

    /RESET

    VCC

    R404k7SMD

    VCC

    GND

    VCCREF1

    RESIN2

    CT3

    GND4

    VCC 8

    SENSE 7

    RESET 6

    RESET 5

    D10

    TL7705 SMDC22100nSMD

    C341u

    GND GNDGND

    C701u

    SMD 35V TA

    not placed

    C28100nSMD

    C29100nSMD

    VDD

    VDD

    AD0

    AD1

    AD2

    AD3

    AD4

    AD5

    AD6

    /RD

    /WR

    GND

    VDD

    GND

    GND10

    VDD11

    ALE12

    A013

    A114

    A215

    DREHZ_IN16

    DREHZ_OUT17

    VDD18

    WR_6X

    19

    WR_7X20TEMP221

    MODE22

    RESET23

    BIT924

    INH_IN25

    TEST26

    D7

    9

    D6

    8

    D5

    7

    D4

    6

    D3

    5

    D2

    4

    D1

    3

    D0

    2

    GND

    1

    VDD

    68

    WR

    67

    RD

    66

    TOUT

    65

    TE

    64

    TEMP5

    63

    TEMP4

    62

    TEMP3

    61

    GND 60

    PH1_O 59

    PH2_O 58

    PH3_O 57

    PH1_U 56

    PH2_U 55

    PH3_U 54

    GND 53

    VDD 52

    TAKT55_IN

    51

    TAKT55_OUT 50TAKT11_IN 49

    PWM_IN 48

    PWM_OUT1 47

    PWM_OUT0 46

    F_IN 45

    F_OUT 44

    Q_OUT27

    Q_IN28

    TAKT

    _029

    TAKT

    _130

    RD

    _4X31

    RD

    _5X32

    TEMPER

    ATUR33

    INTERRUPT34

    GND35

    TEMP136

    TEMP037

    VDD38

    FEHLER39

    UEBERSP40

    INH

    _O041

    INH

    _O142

    GND43

    D12

    ASIC80

    AD7

    ALE

    WR_6X

    GNDVDD

    VDD

    8-BIT-Modus

    PHASE1_OPHASE2_OPHASE3_OPHASE1_UPHASE2_UPHASE3_U

    INHIBIT

    GNDVDD

    GND

    GND

    GND

    R54

    330RSMD

    C3510u

    C3610u

    1 2D17A

    74HC14SMD

    GND VSS

    C27100nSMD

    C6910u

    SMD 25V TA

    C7210u

    SMD 25V TA

    C25100nSMD

    C26100nSMD

    C33100nSMD

    A B C D E F GDP

    R1031kSMD

    V45BC807

    A B C D E F GDP A B C D E F GDP

    R1031kSMD

    V43BC807

    V44BC807

    11 10

    D17E

    to drive lid lock

    to drive compressor

    to drive fans

    signal lid open

    signal over voltage

    signal over current

    temperature signal

    74HC14SMD

    R1021kSMD

    R55

    10kSMD

    56

    D17C

    74HC14SMD

    98

    D17D

    74HC14SMD

    DECKELAUF

    DECKELSCHLOSS

    VCC

    VCC

    R56

    3k3 SMD

    /DECKELAUF

    /DECKEL

    /RESET

    ERROR

    INH

    GND

    GND

    GND

    VDD

    GND

    GND

    GND

    TEMP_0TEMP_1

    TEMPERATUR

    34

    D17B

    74HC14SMD

    R5710kSMD

    /UEBERSPANNUNG

    UEBERSTROM

    GND

    13 12

    D17F

    74HC14SMD

    GND

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    Edition: 01 Wiring Diagram Main Board

    SCHEMATIC DIAGRAMS

    U0

    U+

    R9810k11W

    motor overtempemperatureswitchXB

    1 2

    XB1 F2E11T11G80118 GRAD

    XAlid lock, switches1

    1

    2

    2

    XA1

    R91220R SMD

    not placed

    +15

    overcurrent signal

    UEBERSTROM

    R9612kSMD

    VCC

    1

    2

    3

    4

    D34SFH6106-3SMD

    C63

    100n

    SMD

    C7510uSMD 25V TA

    R9222kSMD

    V52

    P6KE440A

    Brake

    Brems-

    Widerstand

    R87

    2M2SMD

    R101470R9W

    R24470R9W

    C64100nSMD

    R991M

    V51

    ZMM15

    SMD

    C76100u25V SMD

    R88

    10kSMD 1%

    C6547pSMD

    R9568kSMD

    Resistor

    3

    2

    1 5

    6

    7

    4

    D32TL081SMD

    V49BUZ215

    R107

    15RSMD

    GND

    C68

    470p

    SMD

    1

    2

    3

    4

    D33

    SFH6106-3

    SMD

    V47BC817

    GNDV48BC817

    R93

    2k2 SMD

    R94

    2k2 SMD

    C60100nSMDR97

    12k

    SMD

    1

    2

    3

    4

    D35SFH6106-3SMD

    lid open signal

    excess voltage signal

    C6610u

    DECKELAUF

    C7410u

    SMD 25V TA

    /UEBERSPANNUNG

    U-*

    GND

    C6110nSMD

    C6210nSMD

    R100 0R18 2WR8910kSMD 1%

    DC intermediate circuitvoltage control

    U-

    ZK2

    relative excess voltage recognition

    active, if the motor put back powerto the intermediate circuit

    R901M

    absolute excess voltage recognition

    active, if the brake resistor is faulty or ifthe mains voltage is too high

    over current recognition

    Alternatively for V49:HGTP 10N50C1D

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    4 - 19 BIOFUGE fresco

    Wiring Diagram Main Board

    16.12.02 He

    Edition: 01

    SCHEMATIC DIAGRAMS

    C47

    10nSMD

    V27BC807

    GND

    R76100kSMD

    V28BC807

    C5410u

    SMD 25V Ta

    C38100nSMD

    C45100nSMD

    C46100nSMD

    R77

    100kSMD

    GNDGND GND

    C5810u

    GND

    VCC

    GND

    C7310u

    SMD 25V TA

    not placed

    INHIBIT

    R81

    47kSMD

    V29

    LL4148

    1 2

    D26A

    74HC14SMD

    3 4

    D26B

    74HC14SMD

    R64

    220RSMD

    R65

    220RSMD

    1

    2

    3

    4

    D18SFH6106-3SMD

    1

    2

    3

    4

    D19SFH6106-3SMD

    VCCR581M

    SMD

    V40LL4148

    V44

    LL4148V46ZMM12SMD

    C5510u

    SMD 25V TA

    R78

    100k2W

    V21BUZ215

    C481nSMD

    R48

    15RSMD

    U+

    +15

    V22

    BUZ215

    C511nSMD

    R102

    15RSMD

    R591M

    SMD

    V39LL4148

    R66

    220RSMD

    R67

    220RSMD

    1

    2

    3

    4

    D20SFH6106-3SMD

    1

    2

    3

    4

    D21SFH6106-3SMD

    VCC

    5 6

    D26C

    74HC14SMD

    C39220pSMD

    R82

    33kSMD

    V30

    LL4148

    9 8

    D26D

    74HC14SMD

    GND

    PHASE1_O

    PHASE1_U

    C40220pSMD

    V31

    LL4148

    GND

    R68

    220RSMD

    R69

    220RSMD

    1

    2

    3

    4

    D22SFH6106-3SMD

    VCC

    V43

    LL4148V45ZMM12SMD

    R80

    100k2W

    V23BUZ215

    R103

    15RSMD

    MotormotorXM1

    1

    2

    3

    V24BUZ215

    C491nSMD

    R104

    15RSMD

    R601M

    SMD

    R611M

    SMD

    V38LL4148

    C5610u

    SMD 25V TAR70

    220RSMD

    R71

    220RSMD

    1

    2

    3

    4

    D23SFH6106-3SMD

    1

    2

    3

    4

    D24SFH6106-3SMD

    VCC

    13 12

    D26F

    74HC14SMD

    C41220pSMD

    R83

    47kSMD

    V32

    LL4148

    11 10

    D26E

    74HC14SMD

    GND

    PHASE2_O

    PHASE2_U

    C42220pSMD

    R84

    33kSMD

    3 4

    D27B

    74HC14SMD

    GND

    1 2

    D27A

    74HC14SMD

    R73

    220R

    SMD

    1

    2

    3

    4

    D25SFH6106-3SMD

    1

    2

    3

    4

    D28

    SFH6106-3SMD

    V37LL4148

    V41

    LL4148

    V42ZMM12SMD

    R79

    100k

    2W V25BUZ215

    C521nSMD

    R105

    15RSMD

    V26BUZ215

    C501nSMD

    R106

    15RSMD

    R621M

    SMD

    V36LL4148

    C5710u

    SMD 25V TA

    R72

    220RSMD

    R74

    220RSMD

    VCC 1

    2

    3

    4

    D29SFH6106-3SMD

    1

    2

    3

    4

    D30SFH6106-3SMD

    13 12

    D27F

    74HC14SMD

    C43220pSMD

    R85

    47kSMD

    V33

    LL4148

    5 6

    D27C

    74HC14SMD

    GND

    Phase3_O

    Phase3_U

    C44220pSMD

    R86

    33kSMD

    V34

    LL4148

    9 8

    D27D

    74HC14SMD

    GND

    11 10

    D27E

    74HC14SMD

    R75

    220RSMD

    VCC 1

    2

    3

    4

    D31SFH6106-3SMD

    R631M

    SMD

    V35LL4148

    C531nSMD U-*

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    DIASSEMBLY OF INSTRUMENT PARTS

    Edition:01 5-1 Biofuge fresco27.11.02 He

    The index numbers stated in ( ) reappear within the spare part figures (block diagram, schematicdiagram of cooling plant) and the spare part lists.

    5.1 Dismantling the Housing

    5.1.1 Front Panel (100)

    Open the lid. Pull out the mains plug. Pull the unit a little over the table's rim and remove both screws (105) from the bottom of the front

    panel.

    Pull the front panel's lower rim a few centimetre to front and push it vertically out of the upper

    plastic frame. ATTENTION! Do not pull the front panel too much to front! Doing so could tear offthe upper plastic holders!

    For continuing the disassembly remove or disconnect all wires.

    Refitting the front panel is performed analogously in reverse order.

    5.1.2 Lid (130)

    Open the lid. Pull out the mains plug.

    Remove the 4 Allen head screws (135), using a 5 mm key and take off the lid.

    5.1.3 Casing (120)

    Remove the front panel and the lid (see 5.1.1 and 5.1.2).

    Remove the 3 Phillips screws (125) in front and both lateral.

    Press both legs of the torsion spring down and fix them with a 5-mm set screw (or one of the 4Allen head screws) in the bore of the frame.

    Lift the housing at the front and the rear, pull upwards past the legs of the torsion spring and flipover to the front. If the leg is fixed with an Allen head screw, carefully widen the opening of thehousing with a screw driver!

    Pull off mains switch wires and take off the casing inclusive lid sealing.

    Reassemble the device analogously in reverse order.

    Insert the rubber sealing with the gluing seam in front and press on evenly all around

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    DIASSEMBLY OF INSTRUMENT PARTS

    Edition:01 5-2 Biofuge fresco27.11.02 He

    5.2 Replacement of the Sealing Elements

    5.2.1 Motor Rubber Cover (221)Temperature probe and motor are glued to each other! When disassembling the two, damage to eitheror both of them cannot be excluded!

    Open lid, unplug mains plug

    Loosen rotor nut (90) with 10-mm socket wrench (91) and pull out rotor. Press out motor cover from the lower edge to the middle and pull upwards off the plastic flange of

    the motor.

    Carefully pull motor cover and the two insulating washers (224) situated underneath upwards alongthe drive axis and deposit on the side of the temperature probe within the rotor chamber.

    When the motor cover is replaced, the temperature probe must be disassembled and adjustedanew upon reassemble (see section on removal of the temperature probe)!

    When the motor cover is reinserted, the upper rubber sleeve must be pressed in onto the plasticflange (231) of the motor and must be pressed down so that the ring-shaped swelling fits preciselyinto the groove of the flange.

    Check: When finally flanging the inner sleeve lip (covers the upper rim of the plastic flange), theouter edge of the sleeve must slant downward so that condensation water may not enter the motorbearing.

    For fixing and as a protection against torsion, the motor cover plus flange must be secured at theedge with 3 drops of Loctite 495.

    5.2.2 Lid Gasket (123)

    Unscrew the casing (120) and pull it off from the bottom plate (360).

    Separate the sealing's edge from the chamber's rim by pulling it inside.

    Lift off the gasket on this place.

    Work all the way round, until the gasket can be removed completely.

    Assembling instructions

    Push the gasket over the rim of the rotor chamber, starting with the gluing seam in front.

    Work from both sides to the rear, but do not lengthen the gasket by this way.

    While doing so press the sealing edge under the casing's rim. Fold the rim of the rubber sealing over the rotor chamber's opening, starting from front to rear

    Press the casing back again onto the bottom plate and assemble it analogously in reverse order.

    The lid gasket must be completely sit close on the rotor chamber wall!

    The rotor chamber must be sealed completely, when the lid is closed correctly!

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    DIASSEMBLY OF INSTRUMENT PARTS

    Edition:01 5-4 Biofuge fresco27.11.02 He

    5.3 Replacement of Mechanical Components

    5.3.1 Lid Lock Assembly (210)The lid lock assembly encompasses both lid locks with two built-in microswitches each.

    Disassemble housing (see 5.1.3).

    Expose cables for microswitches and lid coils (undo cable tree, unscrew cable clips).

    Remove outer (214) and both inner (215) fastening screws from each of the locking mechanisms. Press microswitches (211) through the frame opening inside and pull both unlocking systems

    upwards and out.

    Note for mounting

    Align rubber spacer (212) and screw locking systems on.

    Do not overtighten the screws!

    The locking pins must not jam! Secure Screws with Loctite 221.

    Fix the cable again to the cable tree and fasten with clips. Cables may not come in between fanblades!

    Once the instrument is installed, check locking mechanism several times by repeated opening andclosing.

    5.3.2 Torsion spring (202

    Disassemble housing (see 5.1.3)

    Remove set screw(s) or Allen head screw (135) from legs in order to disassemble the housing.

    Screw out fastening screw (pan-head 206) of the torsion spring and unbend the spring.

    Remove fastening nut (205) of the swivel leg (203) on the side of the spring and push the shaft(200) to the left until you can remove the spring.

    For removal of the shaft, press right-hand slide bush (204) out and push the shaft to the right until itleaves the left-hand slide bush.

    Lift the shaft on the left and pull obliquely to the left out of the right-hand frame opening.

    For mounting and adjustment of the torsion spring you must follow the instructions in section 2.4,please note also the drawing on page 6-4.

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    DIASSEMBLY OF INSTRUMENT PARTS

    Edition:01 5-4 Biofuge fresco27.11.02 He

    5.4 Replacement of Electrical Components

    5.4.1 Fuses (113) on main board

    Dismantle the front panel (see 5.1.1) and put the device far enough to the rear so that the panelcan be placed without stretching strain of wires on a suitable depot (e.g. box) in front of unit.

    Check the micro fuses and if necessary replace them by equivalent and undamaged ones.

    Perform a test run and search for blowing cause. Reassemble the device analogously in reverse order.

    5.4.2 NV-RAM (113) on main board

    Notice old displayed NV-RAM's version number when powering on (second No. in sequence)

    Depose the front panel as described in 5.4.1.

    ATTENTION - CMOS components! Discharge your body before handling!

    Notice correct position of NV-RAM (8 pins) and pull carefully out of socket.

    Reinsert the new NV-RAM correctly.

    Reassemble the device analogously in reverse order and check the displayed NV-RAM's versionnumber.

    Note! A new NV-RAM version will include parameter changes (e.g. new rotor type or any speed

    limitations etc.) which can be verified in the display.

    5.4.3 Microcontroller (111) on main board

    The microcontroller normally comes with the board. It could be possible that there will be a changesometime necessary when the will be upgrades.

    Notice the old Controller's version number when powering on (first displayed No.).

    Depose the front panel as described in 5.4.1.

    ATTENTION - CMOS components! Discharge your body before handling! Notice the correctController position (CPU) and pull out carefully. ATTENTION - do not damage the socket (use

    cranked pliers)! Reinsert the new Controller chip correctly.

    Reassemble the device analogously in reverse order and check the displayed Controller's versionnumber.

    Note! A new Controller version will include program changes (e.g. modifications of control functions)which might be verified only by measuring performances.

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    DIASSEMBLY OF INSTRUMENT PARTS

    Edition:01 5-6 Biofuge fresco27.11.02 He

    5.4.4 Main Board (110)The key and indication board is part of the main board and cannot be ordered separately!

    Depose the front panel as described in 5.4.1.

    Disconnect all terminal connections as: mains supply XN, compressor box XG, both lid solenoidsXC and both fans XK.

    Pull out all plug connectors as: protecting earth conductor PE, lid switches XA, temperature sensorXU, motor lines XM (1-3) and over-temperature cut-out XB (1+2).

    Remove all fastening screws of the main (117) and indication board (115), remove the washers(116) and subsequently the boards.

    Touch a grounded receptacle to discharge your body before touching the sensitive CMOScomponents.

    Take the NV-RAM (112) out of box (or re-use the old but trouble-free component) and insert it intothe socket of the new main board (see 5.4.2 and 5.4.3).

    The components which is installed at the socket must be removed and scrapped

    Mount the new board onto the front panel, replace the washers and re-tighten all nuts.

    Do not mix up disconnected cables during re-connection!

    Reassemble the device in reverse order and perform a test run, making sure the drive turns in theright direction (see imprinted arrow on rim of rotor chamber)!

    5.4.5 Mains switch (121)

    Remove the casing (see 5.1.3)

    Compress both snap hooks of the mains switch and press the switch from inside out of thecasing's opening.

    Pull off the cable plugs and transfer them to the new mains switch.

    Spread the snap hooks a little, refit the mains switch and assemble the unit completely.

    5.4.6 Mains input socket (361)

    Remove both fixing screws (365) and pull out the socket carefully

    Pull off the cable plugs and transfer them to the new socket.

    Insert the socket and re-tighten it on the tab of the bottom plate.

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    DIASSEMBLY OF INSTRUMENT PARTS

    Edition:01 5-6 Biofuge fresco27.11.02 He

    5.5 Replacement of Drive Components

    5.5.1 Drive Motor (230)

    Remove the casing (see 5.1.3).

    Remove the rubber flange and both isolating disks (see 5.2.1).

    Disconnect the leads for motor XM (1-3) and overtemperature switch XB (1+2)