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The Effect of Sucrose Concentration on the Relative Osmotic Concentration in Potato Tubers Matthew Landry LSU Fall 2011 BIOL 1208 - Biology Lab for Science Majors I
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BIOL 1208
Lab Report
Cover Sheet
I certify that the writing in this assignment is my individual work and is my sole intellectual property. It does not contain the ideas or writing of other individuals/authors.
Matthew Landry______________________ _______11/18/11______________ Author Date
_____20_____ Lab Section #
The Effect of Sucrose Concentration on the Relative Osmotic Concentration in Potato Tubers
Abstract
This lab was conducted in order to determine the molarity of sucrose concentration
where the mass of potato tuber segments does not change, indicating an isosmotic
concentration. By calculating the change of mass of the potato tuber cells prior to
incubation against the mass after incubation in the sucrose solution, our goal was to
determine what type of osmotic concentration was created as compared to the
potato tissues. The results of our lab indicated three different osmotic
concentrations. According to our data, the isosmotic concentration, of a potato tuber
disk is around 0.175M. At this sucrose concentration the sucrose solution is isotonic
relative to the potato tissues. Anything lower than the isosmotic concentration
resulted in a hypertonic concentration. Additionally, anything higher than the
isosmotic concentration resulted in a hypotonic solution.
Introduction
The purpose of a biological membrane is to control the internal environment of a
cell. A cell can be surrounded by millions of molecules at any given time, and it is up
to the membrane to control what enters and what leaves a cell. Water is the single
most important molecule in any living system. Due to water’s small size, it passes
through biological membranes with ease even though water is a polar molecule.
Water molecules are moving across the cell membrane at all times, and the when
more water molecules are traveling in one direction than the other an osmotic
situation occurs. The significance of this lab is to determine the relative osmotic
concentration of disk of potato tubers that are incubated in different sucrose
solutions and to determine at which concentration the mass of the tissues does not
change. This will allow us to better understand of osmotic responses and water
balance in biological systems. Our null hypothesis for the lab was that our treatment
in varying sucrose solutions would have no effect on the potato tuber’s osmotic
concentration. Our alternative hypothesis was that our treatment in varying sucrose
solutions would have an effect on the potato tuber’s osmotic concentration.
Materials and Methods
To begin our lab we gathered six potato tuber segments and used a razor blade to
cut all of the segments, each approximately 4cm in length. Next, we cut one of the
segments into thin disks approximately 2mm in thickness. We placed the other
segments in a moist paper towel to prevent them from drying out. We then placed
the disks from the first segment into a beaker and washed off any surface starch. We
then blotted off the surface water with a dry paper towel. Next, we calculated the
mass of an empty weight boat. We then transferred the potato disks into the boat
and determined the mass to the nearest 0.1g. We then placed the disks into a beaker
with 100mL of deionized water. We then repeated the steps with the remaining five
potato segments; however, we placed the disks from the each new segment into
different sucrose concentrations, 0.1M, 0.2M, 0.3M, 0.4M, and 0.5M. We then
incubated all the disks, in their various concentrations for one hour, swirling the
beakers every ten minutes. At then end of the one-‐hour period, we removed the
disks from their concentration. Next, we blotted off any surface water with a dry
paper towel. We placed the disks into the weight boat and measured the mass on a
scale. Finally, we calculated the percent change in mass by diving the change in
mass by the initial mass then multiplying by one hundred.
Results
Based on the data from the six treatments a regression line was formed. The percent
change in mass of the potato cells is positive when incubated in concentrations of
0.0, 0.1, and 0.2 M of sucrose. At these three concentrations, a hypertonic
environment was observed within the cell. The percent change in mass of the potato
cells is negative when incubated in concentrations of 0.3, 0.4, and 0.5M of sucrose.
At these three concentrations, a hypotonic environment was observed within the
cell. According to the data, a line of best fit crosses the x-‐axis where an isotonic
relative osmotic concentration is achieved. An isotonic solution was achieved when
the sucrose solution is around 0.175 M. Based on the results from the experiment,
our alternative hypothesis was correct.
Based on the data, the concentration of sucrose at approximately 0.175 M shows no change in the weight of the potato tuber disks indicating an isotonic relationship
between the disks and solution.
Discussion
According to our data, the relative isosmotic concentration of a potato tuber disk is
around 0.175M, which is where the sucrose concentration surrounding the cells
creates neither a hypotonic or hypertonic environment. If the concentration of
sucrose is greater on the outside of the cell, then the net movement of water is out of
the potato cells. A hypertonic situation occurs, and the tuber cells experience a loss
in mass .In plant cells, the loss of water from a cell causes the cell to shrivel or
-30.00%
-25.00%
-20.00%
-15.00%
-10.00%
-5.00%
0.00%
5.00%
10.00%
15.00%
0 0.1 0.2 0.3 0.4 0.5 0.6
Percent Δ∆ in Mass (g)
Concentration of Sucrose Solution (M)
The Effect of Sucrose Concentration on the Relative Osmotic Concenctration in
Potato Tubers
undergo plasmolysis. This trend can be viewed when the sucrose concentration was
raised over 0.3M. If the concentration of sucrose is greater on the inside of the cell,
then the water enters the potato disks. A hypotonic situation occurs outside of the
cells, and the tuber cells experience a gain in mass, as the net movement of water is
into the cells. In plant cells, this trend is known as turgid pressure. This trend can be
viewed when the sucrose concentration is lowered from 0.2M to 0.0M of sucrose.
For this experiment the expected isosmotic concentration is 0.3 molar; however, our
data does not support this. Some possible sources of error in our experiment include
missteps during setup and preparation for the experiment, inaccurately measuring
the mass, or other factors.