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7/25/2019 Biomolecular Simulation: A Computational Microscope for Molecular Biology
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Biomolecular Simulation:A Computational Microscopefor Molecular Biology
Ron O. Dror,1 Robert M. Dirks,1 J.P. Grossman,1
Huafeng Xu,1 and David E. Shaw1,2
1D. E. Shaw Research, New York, New York 10036; email: [email protected]@DEShawResearch.com
2Center for Computational Biology and Bioinformatics, Columbia University, New York,New York 10032
Annu. Rev. Biophys. 2012. 41:42952
TheAnnual Review of Biophysicsis online atbiophys.annualreviews.org
This articles doi:10.1146/annurev-biophys-042910-155245
Copyright c2012 by Annual Reviews.All rights reserved
1936-122X/12/0609-0429$20.00
Keywords
molecular dynamics, millisecond timescale, Anton, protein folding, dru
binding
Abstract
Molecular dynamics simulations capture the behavior of biological ma
molecules in full atomic detail, but their computational demands, combwith the challenge of appropriately modeling the relevant physics, have
torically restricted their lengthand accuracy. Dramatic recentimprovemin achievable simulation speed and the underlying physical models
enabled atomic-level simulations on timescales as long as milliseconds
capture key biochemical processes such as protein folding, drug bindmembrane transport, and the conformational changes critical to pro
function. Such simulation may serve as a computational microscopevealing biomolecular mechanisms at spatial and temporal scales that
difficult to observe experimentally. We describe the rapidly evolving of the art for atomic-level biomolecular simulation, illustrate the typ
biological discoveries that can now be made through simulation, and dischallenges motivating continued innovation in this field.
429
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Conformationalchange: a transitionbetween twoalternative structuresof a flexiblebiomolecule such as aprotein
Molecular dynamics(MD) simulation: asimulation in whichthe positions andvelocities of atoms arecomputed usingNewtons laws ofmotion
Contents
I N T R O D U C T I O N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3 0RECENT ADVANCES IN SIMULATION METHODOLOGY................... 433
Accessing Longer Timescales . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 433Enhanced Sampling and Coarse-Graining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 436
Improving Force Field Accuracy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 436
SIMULATION AS A TOOL FOR MOLECULAR BIOLOGY . . . . . . . . . . . . . . . . . . . . . 437Conformational Changes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437
Membrane Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 439Protein Folding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 441
Ligand Binding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 442FUTURE FRONTIERS OF BIOMOLECULAR SIMULATION. . . . . . . . . . . . . . . . . . 444
Drug Design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 444Protein Design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 445
Modeling Nucleic Acids . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 445Simulation of Complex Biological Structures. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446
Enabling Longer and Cheaper Simulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446
More Accurate Biochemistry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 446
INTRODUCTION
Over the past half-century, breakthroughs in structural biology have provided atomic-resolution
models of many of the molecules that are essential to life, including proteins and nucleic acids.
Although static structures determined through crystallography and other techniques are tremen-dously useful, the molecules they represent are, in reality, highly dynamic, and their motions are
often critical to their function (Figure 1). Proteins, for example, undergo a variety of conforma-tional changes that allow them to act as signaling molecules, transporters, catalysts, sensors, and
mechanical effectors. Likewise, they interact dynamically with hormones, drugs, and one anotherStatic structural information might be likened to a photograph of a football game; to understand
more readily how the game is played, we want a video recording.A variety of experimental techniques can provide information about the dynamics of proteins
and other biomolecules, but they are generally limited in their spatial and temporal resolutionand most report ensemble average properties rather than the motion of individual molecules
(Figure 2). An attractive alternative, in principle, is to model atomic-level motions computa-tionally, based on first-principles physics. Although such simulations have been an active area of
research for decades (55), their computational expense, combined with the challenge of develop-
ing appropriate physical models, has placed restrictions on both their length and their accuracyThe past few years have seen great progress in addressing these limitations, making simulations a
much more powerful tool for the study of biomolecular dynamics. This review describes severalimportant recent advances in simulation methodology and offers an overview of what is currently
possible with biomolecular simulation.The quantum mechanical behavior of molecules at a subatomic level is described by the time-
dependent Schrodinger equation, but a direct solution to this equation is in practice computation-ally infeasible for biological macromolecules. The standard method for simulating the motions of
such molecules is a technique known as all-atom molecular dynamics (MD) simulation, in which
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b Ligand bindinga Transport across membrane
c Conformational change d Protein folding
Figure 1
Examples of biomolecular processes that have been examined using molecular dynamics (MD) simulations.(a) Transport of small molecules across the cell membrane. (b) Binding of drugs to their target proteins.(c) Conformational transitions in proteins. (d) Protein folding.
Force field: enerfunction used tocompute the forceacting on atoms (dto interatomicinteractions) durinMD simulation
the positions and velocities of particles representing every atom in the system evolve according
to the laws of classical physics. The forces acting on these particles are computed using a modelknown as a force field, which is typically designed based on a combination of first-principles
physics and parameter fitting to quantum mechanical computations and experimental data.
Although MD simulation does not model the underlying physics exactly, it can provide a suf-ficiently close approximation to capture a wide range of critical biochemical processes. The popu-
larity of such simulations is illustrated by the fact that they account for a majority of the computertime devoted to biomedical research at National Science Foundation supercomputer centers.
Although we briefly touch on approaches that represent molecules at a coarser or finer level ofdetail, or that evolve positions in a nonphysical manner, all-atom MD simulations constitute the
principal focus of this review.Historically, the timescales accessible to MD simulation have been shorter than those on
which most biomolecular events of interest take place, thus limiting the applicability of these
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Proteintranslation
Activetransport
Actionpotential
Solute permeationProtontransfer
Side chainip
Domainmotion
2 structureformation
Channelgating
Lightabsorption
Electrontransfer
1015 1012 109 106 103 100 103 Static
Time (s)
Length(n
m)
101
100
101
103
104
102
Increasingresolution
Atoms
-sheets
-helices
Proteins
Assemblies
Organelles
Cells
All-atommolecular dynamics
simulations
Electrophysiology
FRET
X-ray
NMR
EM
AFM/optical tweezers
Figure 2
Spatiotemporal resolution of various biophysical techniques. The temporal (abscissa) and spatial (ordinate)resolutions of each technique are indicated by colored boxes. Techniques capable of yielding data on singlemolecules (as opposed to only on ensembles) are in boldface. NMR methods can probe a wide range oftimescales, but they provide limited information on motion at certain intermediate timescales, as indicatedby the lighter shading and dashed lines. The timescales of some fundamental molecular processes, as well ascomposite physiological processes, are indicated below the abscissa. The spatial resolution needed to resolvecertain objects is shown at the right. Adapted from Reference 19. Abbreviations: AFM, atomic forcemicroscopy; EM, electron microscopy; FRET, Forster resonance energy transfer; NMR, nuclear magneticresonance.
simulations. Events such as protein folding, proteindrug binding, and major conformational
changes essential to protein function typically take place on timescales of microseconds to mil-liseconds (Figure 2). MD simulations, by contrast, were until recently generally limitedin practice
to nanosecond timescales. Simulations of even a few microseconds required months on the mostpowerful supercomputers available, and longer simulations had never been performed. Recent
advances in hardware, software, and algorithms have increased the timescales accessible to simula-
tion by several orders of magnitude, enabling the first millisecond-scale simulations and allowingMD to capture many critical biochemical processes for the first time.
The other major factor limiting the applicability of MD has been the accuracy of the force fieldmodels that underlie the simulations. A number of improved force fields have been introduced
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Parallelcomputation: usmultiple cooperatprocessors to perfa computation fastthan would be pos
with a single procMoores law: a trdating back to 196which the logic deon computer chipdoubles approximevery two years
over the past several years, and the longer timescales now accessible to MD simulations have
allowed more extensive validation of these force fields against experimental data.
We begin by summarizing the fundamentals of MD simulation and certain recent methodolog-ical and technological advances that have expanded its applicability. We then review the state of
the art in terms of the types of biological discoveries one can make through simulation, providinga number of recent illustrative examples. Finally, we discuss several classes of important problems
that MD could potentially address in the coming years and the methodological advances that mayhelp solve them.
RECENT ADVANCES IN SIMULATION METHODOLOGY
Although the speed and accuracy of all-atom MD simulations has improved substantially over the
past few years, the basic form of such simulations has endured (1). Each atom in the systemfor
example, a protein and the water surrounding itis represented by a particle (or, in certain cases,multiple particles). The simulation steps through time, alternately computing the forces acting
on each atom and using Newtons laws of motion to update the positions and velocities of all theatoms. Commonly used biomolecular force fields express the total force on an atom as a sum of
three components: (a) bonded forces, which involve interactions between small groups of atomsconnected by one or more covalent bonds; (b) van der Waals forces, which involve interactions
between all pairs of atoms in the system but which fall off quickly with distance and are generallyevaluated only for nearby pairs of atoms; and (c) electrostatic forces, which involve interactions
between all pairs of atoms and fall off slowly with distance. Electrostatic interactions are computedexplicitly between nearby pairs of atoms, whereas long-range electrostaticinteractionsare typically
handled via one of several approximate methods that are more efficient than explicitly computinginteractions between all distant pairs of atoms.
Accessing Longer Timescales
MD simulations are computationally demanding for two reasons. First, the force calculation ateach time step requires substantial computationroughly one billion arithmetic operations for a
system with one hundred thousand atoms. Second, the force calculation must be repeated manytimes. Individual steps are limited to a few femtoseconds by fast atomic vibrations, so simulating a
millisecond of physical time requires nearly one trillion time steps. On a single high-end processorcore, such a simulation would take thousands of years to complete.
To make matters worse, using many computer processors in parallel to accelerate a simulationis challenging. Parallelizing a force calculation across multiple processors requires that those
processors communicate with one another, and the amount of communication required increaseswith the number of processors. Beyond some point, adding more processors actually slows down
the calculation. Furthermore, the force calculations in a single simulation must be performed
sequentially, because the atom positions that serve as input for one force calculation depend onthe results of the previous one. The difficulty of parallelizing an MD simulation implies that the
transistor density improvements predicted by Moores law do not automatically lead to improvedMD performance, because in recent years higher transistor density has translated to more processor
cores per chip, not faster individual processor cores.In spite of these challenges, the recent performance improvements in MD simulations have
far outpaced Moores law. In half a decade, the raw performance of state-of-the-art simulationsincreased by over three orders of magnitude (Figure 3). As recently as 2007, the longest published
all-atom MD simulation of a protein was 2 s; in 2009, the first millisecond-long simulation was
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Performance
(sim
ulatednsday1)
Year
1
10
100
1,000
10,000
2004 2005 2006 2007 2008 2009
Moore's lawtrend
Fastest reported
all-atom MD
simulation
Fastest reportedall-atom MDsimulation
Figure 3
Fastest reported all-atom molecular dynamics (MD) simulations from 2004 to 2009 (blue line). The simulatedsystems ranged from 14,000 to 92,000 atoms, and different simulations were performed using differentparameters, so this data is not intended to be a direct comparison of MD hardware and software systems.Nonetheless, an overall performance trend is evident, substantially exceeding the Moores law growth trendin processing power (black line). The leftmost data point is from a 512-processor simulation using NAMD
(65); the rightmost data point is from a 512-chip simulation on Anton (82). The remaining data points arefrom simulations run using Blue Gene/L (25) and Desmond (9, 14).
published (Table 1). These improvements are attributable to a variety of hardware, software, and
algorithm innovations, which we discuss below.
Parallelization across general-purpose computer chips. Software that parallelizes MD force
calculations across multiple computer processors has existed for two decades (69) but has becomemuch more efficient and scalable in the past several years. IBMs Blue Matter code for its Blue
Gene/L general-purpose supercomputer has been scaled up to 32,768 cores (25). The widelyused MD codes NAMD (65), GROMACS (31), and AMBER (12) have all substantially improved
their parallel performance in recent years. These packages can now deliver performance of over100 ns day1 on commodity computer clusters, with the number of processors required to achieve
this performance scaling roughly linearly with the number of atoms in the system. Desmond, a
software package for commodity clusters developed at D. E. Shaw Research, allowed simulationsnearly an order of magnitude faster than previously possible on the same hardware (9) and has
achieved performance approaching 500 ns day1 (14).These improvements in parallel scalability and efficiency have been possible thanks to a number
of algorithmic innovations, particularly in methods for reducing the communication requirements
Table 1 Longest reported all-atom molecular dynamics simulations from 2006 to 2009
Year Length (s) Protein Platform Reference
2006 2 Rhodopsin Blue Gene/La 54
2007 2 Villin HP-35 GROMACSb 22
2008 10 WW domain NAMDb 27
2009 1,031 BPTI Anton 82
aThis simulation used IBMs Blue Matter software.bThese simulations were performed on a commodity computer cluster with the specified software.
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GPU: graphicsprocessing unit
Special-purposeparallelarchitectures:computerarchitectures desigfor a specific task,often allowing succomputers to comthat task much fasthan general-purpcomputers
Anton: a special-purpose parallelsupercomputerdesigned by D. E.Shaw Research toenable fast MDsimulations
between chips during a simulation. The class of neutral territory methods (9, 10, 25, 81, 86), for
example, substantially reduces the amount of data that must be exchanged between processors in
order to compute range-limited particle interactions.
Graphics processing units. Originally designed specifically to accelerate the rendering of three-
dimensional graphics, graphics processing units (GPUs) have become increasingly popular for
general-purpose scientific computation thanks to their ability to perform large numbers of iden-tical computations in parallel on a single chip. Several MD implementations have been ported to
GPUs (2, 30, 29, 66), and a simulation on one or a few GPUs often rivals the performance of asimulation on a small- to moderate-sized computer cluster. Unfortunately, efficiently parallelizing
across many GPUs is difficult, because communication between GPUs remains slower than com-munication between general-purpose processors; as a result, clusters of GPUs have been unable to
match the performance of large standard clusters. GPUs offer an excellent price-to-performanceratio, however, enabling reasonably fast simulations at a cost substantially lower than that for a
cluster of general-purpose processors.
Special-purpose parallel architectures. By far the greatest recent speedups have been achievedthrough the use of special-purpose chips, in combination with new parallelization algorithms
and software. In particular, a recently developed machine named Anton can perform all-atomMD simulations at up to 20 s day1, approximately two orders of magnitude faster than the
simulation rates generally achieved by any other hardware/software combination (82).Anton is able to achieve this speed because it is designed specifically for MD simulations. The
entire computation is performed on special-purpose chips developed at D. E. Shaw Research,
which are directly connected to one another in a custom network (Figure 4). Each Anton chipcontains an array of arithmetic units hardwired for computing particle interactions, enabling a
single Anton chip to compute interactions hundreds of times faster than a commodity proces-sor core (82). Each chip also contains a dozen programmable processor cores, customized for
MD, which accelerate the remainder of the computation. In addition, specialized data-movement
a b
Figure 4
Anton, a special-purpose computer for molecular dynamics designed by D. E. Shaw Research, has performed all-atom proteinsimulations over one hundred times longer than any published previously. ( a) A single Anton chip. (b) The first Anton machine,comprising 512 Anton chips connected through a specially designed network.
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Enhanced sampling:algorithms devised tospeed up theexploration ofmolecularconformations by
altering the physics ofthe system
operations support fast communication between small on-chip memories, eliminating the memory
cache hierarchy that typically consumes the majority of the area on commodity chips.
Several algorithmic advances also contribute to Antons performance. A specific neutral terri-tory method (81) was designed for Anton and is directly implemented within its specialized parti-
cle interaction hardware. Anton computes long-range electrostatic forces using the Gaussian splitEwald method (79) rather than the more commonly used particle mesh Ewald method, allowing
a significant portion of the long-range electrostatics computation to be performed by the samespecialized hardware that handles particle interactions. Finally, the communication patterns in
Antons MD software, which differsignificantly from those in other parallel MD software packagesare designed to take advantage of Antons specialized low-latency mechanisms for communication
between and within chips (18).Several previous projects, including FASTRUN (24), MD Engine (92), and MDGRAPE (90),
have built special-purpose hardware to accelerate the most computationally expensive elements
of an MD simulation. Although such hardware reduces the effective cost of simulating a givenperiod of biological time, the speedup achieved through parallelization across many such chips is
limited by the remainder of the computation as well as the communication required, precludingindividual simulations on multi-microsecond timescales.
Anton has enabled all-atom MD simulations of proteins of more than a millisecond in lengthover 100 times longer than any such simulation reported using other hardware. With Anton it
becomes possible, forthe first time, to directly observe in simulation various importantbiochemicaprocesses that occur on timescales greater than a few microseconds.
Enhanced Sampling and Coarse-Graining
A variety of methods can be used in combination with MD simulation to investigate events that
occur on timescales that remain inaccessible to direct all-atom MD simulation. Zuckerman (106)
provided a thorough review of such enhanced sampling methods in Volume 40 of the AnnualReview of Biophysics, in which he concluded that No other method can routinely and reliably
outperform MD by a significant amount. These methods prove essential in certain situationshowever, as illustrated by several of the case studies in the following section.
One can also sidestep communication bottlenecks by performing many short simulations inparallel. A particularly impressive example is the Folding@Home project (5), which has obtained
significant scientific results by using over 400,000 personal computers (PCs) to simulate manyseparate molecular trajectories, each limited to the timescale accessible on a single PC. The
solution of many important problems, however, is greatly facilitated by the availability of longindividual trajectories.
Finally, one can often substantially accelerate simulations at the cost of reduced accuracyby employing simplified system representations, such as coarse-grained models, in which each
simulated particle represents several atoms, or implicit solvent models in which water atoms are
replaced by a continuum representation. Models of both types have seen substantial developmentin recent years (13, 58).
Improving Force Field Accuracy
Long-timescale simulations place more stringent demands on force fields; a force field that proves
sufficient for short-timescale simulations may not be sufficient at longer timescales. Fortunately,the past several years have seen substantial improvements in force fields for biomolecular simula-
tion. Historically, force field parameters were determined using quantum mechanical calculations
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G-protein-couplreceptors (GPCRa family oftransmembraneproteins that transsignals into cells a
represent the largclass of drug targe
and condensed phase experimental data for small molecular fragments. Recently, force field de-
velopment has come to increasingly rely on experimental data for proteins and other biological
macromolecules, as improvements in both simulation speed and experimental methods have ledto an overlap in the timescales accessible through the two approaches.
Although the functional forms of the most widely used force fields have remained largelyunchanged, their parameters have recently undergone a number of adjustments. The Amber
force field, for example, incorporated changes to parameters associated with torsional angles ofthe protein backbone, first to improve fits to quantum calculations (32) and then to achieve
better agreement between secondary structure preferences observed in long MD simulations ofpolypeptides and corresponding NMR measurements (7). Amber protein side chain torsions were
also adjusted to better match both quantum calculations and NMR data (51). Adjustments tobackbone and side chain torsions were also incorporated into the CHARMM force field (53, 67),
as were modifications to the charge distributions of ionizable amino acid residues (67). Recent
studies have also resulted in improved parameters for lipids in CHARMM (42) and for smalldrug-like molecules in the CHARMM, Amber, and OPLS-AA force fields (4, 96, 98).
A recent study exploited long-timescale MD simulations on Anton to evaluate a number ofprotein force fields through a systematic comparison with experimental data (49). Criteria in-
cluded the ability of each force field to fold small proteins to their native structures, to predict thesecondary structure propensities of polypeptides, and to reproduce NMR data reporting on the
structure and dynamics of folded proteins. The results indicated that the force fields examinedhave consistently improved overthe past decade, andthat themost recentversions providean accu-
rate description of many structural and dynamic properties of proteins. The study also highlightedcertain shortcomings: None of the force fields, for example, were able to accurately capture the
temperature dependency of the secondary structure propensities. It is an open question whether
the ongoing parameterization of existing functional forms will be sufficient to further improveforce fields. Substantial efforts are under way to develop force fields with more sophisticated
functional forms, including polarizable force fields (36, 70), which capture the redistribution ofelectrons around each atom in response to changes in environment.
SIMULATION AS A TOOL FOR MOLECULAR BIOLOGYIn this section, we illustrate the utility of modern MD simulation as a biological research tool
through a number of recent case studies, many of which are drawn from our own work, involvingconformational changes in proteins, transport across membranes, protein folding, and the binding
of ligands to proteins (Figure 1).
Conformational Changes
Under physiological conditions, proteins and other biomacromolecules constantly move from
one structural state to another, and their function and regulation depend on these conformationalchanges. MD simulations are often used to identify novel conformations, to capture the transi-
tional pathways between conformations, to determine equilibrium distributions among differentconformations, and to characterize changes in conformational distribution as a result of mutation
or ligand binding. We provide several examples involving G-protein-coupled receptors (GPCRs)and kinases.
GPCRs represent the largest class of drug targets: One-third of all marketed drugs act bybinding to a GPCR and either triggering or preventing receptor activation, which involves
a transition from an inactive receptor conformation to an active conformation that causes
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2-adrenergicreceptor (2AR): anarchetypal GPCR anda target of betablockers and betaagonists
G-protein-mediated signaling. The past few years have witnessed the determination of the first
several crystal structures of ligand-activated GPCRs, beginning with the 2-adrenergic receptor
(2AR). MD has addressed several key questions raised by these structures about the conforma-tions of inactive states and the mechanism of receptor activation (16, 17, 52, 62, 72, 73, 95).
An MD simulation study by Dror et al. (16) identified a previously unobserved inactive con-formation of2AR, resolving an apparent contradiction between experimental results: A network
of salt bridges known as the ionic lock, suggested by biochemical experiments to stabilize theinactive state of2AR and other GPCRs, was disrupted in the inactive state crystal structures
(46). In microsecond-timescale simulations of inactive2AR, the receptor transitions repeatedlybetween two conformational states, one with the lock broken and one with it formed. In simu-
lations of wild-type 2AR, the lock-formed conformation predominates, in agreement with thebiochemical data, but in simulations of the modified protein used for crystallographic structure
determination, the lock-broken conformation predominates, explaining the crystallographic re-
sult. Both lock-broken and lock-formed conformations were subsequently observed in a crystalstructure of a closely related receptor (60), lending support to these computational predictions.
More recent simulations on Anton (73) captured spontaneous transitions of2AR from an activeto an inactive conformation, addressing a puzzle posed by two recent crystallographic structures of
2AR bound to two differentagonists (ligands that cause activation). One of these structures, whichalso has a G-protein-mimetic nanobody bound to its intracellular surface, appears to represent an
active conformation (71). The other, which is bound to an agonist but lacks the nanobody, is almostidentical to the previously solved inactive structure (73). Is this surprising structural difference due
to differences between the agonists or the crystallized receptor constructs, or might it be due to thepresence or absence of the G-protein-mimetic partner? In multi-microsecond simulations of2AR
initiated from the nanobody-bound active structure, but with the nanobody removed, the agonist-bound receptor spontaneously transitioned to a conformation that closely matched the inactive
crystal structure. Taken together, these simulations and the crystal structures suggest that, even
with an agonist bound, the majority of the 2AR population remains in an inactive-like conforma-tion until a G-protein or G-protein-mimetic nanobody binds, stabilizing the active conformation
These simulationswhich represent the first in which a GPCR transitions spontaneouslybetween crystallographic conformations representing functionally distinct statesalso served to
characterize the atomic-level activation mechanism of2AR (17). They showed that the extra-cellular drug-binding site is connected to the intracellular G-protein-binding site via a loosely
coupled allosteric network, comprising three regions that can switch individually between dis-tinct conformations. The simulations revealed a key intermediate conformation on the activation
pathway and suggestedsomewhat counterintuitivelythat the first structural changes duringactivation often take place on the intracellular side of the receptor, far from the drug-binding site.
These results may provide a foundation for the design of drugs that control receptor signalingmore precisely by stabilizing specific receptor conformations.
MD simulations have also shed light on the function and regulation of kinases, a class of
enzymes that are actively pursued as therapeutic targets for cancer and autoimmune diseases. Theactivity of a typical kinase is regulated by changes to its preference for the active and various
inactive conformations. Mutations to kinases often favor the wrong conformations, leading toaberrant signaling and consequently disease. A number of studies have used MD simulations to
characterize conformational changes in kinases (6, 23, 80, 100), yielding predictions that were inagreement with subsequent experimental measurements (80) and insights that led to the design
of new experimental methods (76).Faraldo-G omez & Roux (23) used MD simulations to characterize the regulation of Src family
tyrosine kinases, which depends on an inactivation process in which the auxiliary domains (known
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Membranetransport:the movement ofmolecules across amembrane, usuallyfacilitated by a
transmembraneprotein
as SH2 and SH3) of a kinase assemble onto its catalytic domain, preventing catalysis. What
makes such assembly robust and fast, so that kinases can be reliably and quickly turned off? To
answer these questions, the authors used an enhanced sampling technique known as umbrellasampling (44) to characterize the relative free energies of various conformations connecting the
disassembled and assembled states. The simulations indicated that the SH2SH3 construct hasan intrinsic propensity to adopt conformations primed for association with the catalytic domain,
thus favoring and accelerating formation of the assembled (inhibitory) state. Their results alsosuggested that the SH2SH3 connector is more than a passive link between the domains; rather,
it is responsible for their propensity toward the assembly-ready conformation, explaining theexperimental observation that mutations in the connector region increase the constitutive activity
of the kinase.
Membrane Transport
Transport of various substrates across the cell membrane is vital both to maintaining a cells con-
stitution and to transmitting biochemical signals. The transport efficiency and substrate selectivityof carrier proteins often depend critically on the detailed spatial configuration of the atoms along
the transport pathway as well as their subtle movement during the transport process. MD sim-ulation, with its unique ability to simulate and record the movement of individual atoms at very
fine temporal and spatial resolutions, lends itself naturally to the study of transport processes. Inthe past decade, MD simulations have been applied to investigate a number of transporters and
channels, including aquaporins (35, 91), ion channels (8, 34, 63), and active transporters (3, 21).These studies have shed light on many mechanistic questions: How do the channels achieve a fast
rate of substrate permeation? How do the transporters affect selectivity for their substrates? Howis transport regulated in response to various stimuli?
Potassium channels, which allow potassium ions to move passively through the cell membrane,
are essential for the transmission of nerve impulses and represent an important target for thetreatment of diseases ranging from Alzheimers to diabetes. A longstanding puzzle about these
channels is why they let potassium ions, but not smaller sodium ions, pass through. Crystal struc-tures suggest that the narrowest region of a potassium channel, known as the selectivity filter,
has a geometry that snugly fits potassium ions, but the difference between the radii of potassiumand sodium ions (0.38 A) is smaller than the thermal fluctuations of the atomic positions in the
selectivity filter (0.75 A). Noskov et al. (63) and Bostick & Brooks (8) addressed this question byusing MD simulations to examine several hypothetical variants of the real selectivity filter. In both
studies, the authors computed the difference in the binding free energies of sodium and potassiumions to the selectivity filter and explored how this difference varied when they artificially adjusted
the physical properties of the filter. Both studies suggested that selectivity was a robust featureof the filter that did not depend on its precise geometry. Instead, selectivity was a consequence of
the dipole moment of the carbonyls coordinating the ions in the selectivity filter, the coordination
number, and the thermal fluctuations in the filter.Recent advances in simulation speed have allowed the first direct, atomic-resolution ob-
servations of ion permeation and pore domain closure in a voltage-gated potassium channel(Figure 5). Using unbiased microsecond-timescale MD simulations at various transmembrane
voltages, Jensen et al. (34) followed the permeation of hundreds of potassium ions through thechannel. The authors identified the transitions between microscopic states that underlie the per-
meation of an individual ion, thereby supplying atomistic detail of the long-postulated knock-onconduction mechanism, in which translocation of two selectivity-filter-bound ions is driven by a
third, incoming ion.
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Closed andfully dewetted
Closed andpartially dewetted
0
10
10
5
5
Ionpositio
nalongporeaxis()
0.1 0.2 0.3 0.4
Time (s)
H
2
OH2O
K
K+
Time
a Ion transport at positive transmembrane potential
Open andwetted
b Negative transmembrane potential induces channel closure
Figure 5
Simulation of ion permeation and gating in a potassium channel. ( a) Potassium ions permeated outward (inthe figure, upward) through the selectivity filter when the transmembrane potential was positive. Individualions paused at well-defined sites within the filter, as shown by the representative traces in green. ( b) Whenthe transmembrane voltage was reversed, the hydrophobic cavity dehydrated, causing it to collapse and thusclose the channel to conduction. Figure adapted from Reference 34.
Moreover, Jensen et al. observed channel closuregating of the potassium channel poredomainat negative voltages (Figure 5). Closure took place by means of a previously hypoth-
esized, but unobserved (for ion channels) mechanism, called hydrophobic gating, in which thehydrophobic cavity adjacent to the selectivity filter dehydrated, causing the open pore domain to
collapse into a closed conformation. This mechanism provides a molecular explanation for theexperimental observation that the channel conductance is sensitive to the osmotic pressure. In
particular, the change in volume upon channel closure has been measured experimentally, and
it corresponds to the volume of 4050 water molecules, closely matching the number of watermolecules expelled from the pore cavity upon channel closure in the simulations (105).
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Folding pathwaya sequence ofintermediatestructures visited bprotein as it transifrom a disordered
to its native state
MD simulations have also been used to deduce the mechanism of the sodium proton antiporter,
NhaA (3), a transporter that moves sodium ions and protons in opposite directions across the cell
membrane. Arkin et al. (3) performed a series of simulations in which they systematically variedthe initial position of the sodium ion, as well as the protonation states of two aspartate residues
Asp163 and Asp164critical for antiporting function. These simulations suggested that Asp164serves as the binding site of sodium ion, with its protonation state determining whether the ion will
remain bound or be released into the membrane, and that Aps163 acts as an accessibility controlsite, determining whether the ion will be released to the inside or outside of the cell. Although
the simulations (100 ns each) were much shorter than the complete antiporting cycle (1 ms),they allowed formulation of a complete transport mechanism, which was substantiated through
experiments on NhaA mutants.
Protein Folding
Protein folding actually represents two challenges: Given only a proteins amino acid sequence,
(a) determine the native structure of the protein, and (b) elucidate the pathways by which it folds tothat structure. MD can potentially address both challenges (87), but it is particularly well suited for
revealing folding pathways. Many computational (43) and experimental methods directly predict
or determine protein structure, but few techniques allow direct observation of the dynamics of afolding event in atomic detail. Given an accurate force field and sufficient simulation time, MDcan produce atomic-level trajectories of spontaneous folding events (22, 28, 37, 47, 8385, 97), as
well as unfolding events (93). Such a microscopic view can shed light not only on the structure andstability of the folded state, but also on the heterogeneity of folding pathways, the rate-limiting
steps on these pathways, the nature of misfolded states, and other complex features of the proteinfolding process.
Improvements in both simulation speed and force field accuracy recently enabled Lindorff-
Larsen et al. (50) to simulate repeated folding and unfolding events for a structurally diverse setof 12 small, fast-folding proteins, using a single force field. All 12 proteins folded to structures
closely resembling those determined experimentally (Figure 6). The ability of simulations to
identify the native structures is itself noteworthy, suggesting that MD may eventually serve as aviable method for predicting or refining the structures of arbitrary proteins. The most immediateutility of these simulations, however, is in allowing direct observation of the protein folding
process.Comparing the behavior of these 12 proteins suggested unifying principles for protein folding,
at least for small, fast-folding proteins, and allowed the authors to address several long-standingquestions regarding the mechanisms of protein folding (88). Most of the proteins studied, for
example, consistently fold along a single dominant route, with local structures forming in an order
that largely corresponds to the stability of those structures in the unfolded ensemble. In addition,a few long-range contacts typically form early in the folding process and help establish a nucleus
to guide formation of the rest of the structure.
MD can also help guide wet-lab protein folding experiments (45). Piana et al. (68), for exam-ple, used insights gained from long simulations of a WW domain to suggest a triple mutationthat should reduce the main energy barrier on the folding pathway and thus accelerate fold-
ing. Temperature-jump experiments confirmed this prediction, establishing this mutant as thefastest folding-sheet protein knowna conclusion made more noteworthy because substantial
effort had previously been dedicated to maximizing the folding rate of this WW domain throughmutagenesis (61).
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Chignolin BBATrp-cage Villin
WW domain NTL9 BBL Protein B
Homeodomain Protein G 3D repressor
Figure 6In simulations with a single force field, 12 structurally diverse proteins fold spontaneously to a structure(blue) closely resembling that determined experimentally (red). The simulation snapshots were chosenautomatically based on a clustering analysis that did not exploit knowledge of the experimental structure.The total simulation time per protein ranged from 104 to 2,936 s, allowing observation of at least 10folding and 10 unfolding events for each protein. Figure adapted from Reference 50.
Ligand Binding
Interactions between proteins and small-molecule ligands play a key role in intercellular signaling
and, when the ligands are drugs, in the treatment of disease. Ligands typically affect proteinfunction by directly blocking the active site of a protein or by causing the protein to adopt a
functionally altered conformational state.Thanks to recent advances in accessible timescales, it is now possible to perform MD simula-
tions in which ligands bind spontaneously to proteins without any prior knowledge of the bindingsite (20, 33, 78). In work by Shan et al. (78) on inhibitors binding to Src kinase, and by Dror et al.
(20) on beta blockers and a beta agonist binding to two GPCRs, simulated drug molecules diffusedextensively about the protein before discovering their binding site and binding in a location and
conformation that match crystallographic observations almost exactly (Figure 7). These results
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(S)-dihydroalprenolol
NH2+
HO
O
0.51 s 0.68 s 0.80 s 1.88 s 3.68 s
5
10
15
20
25
30
Ligandrmsd()
0 1 2 3 4 Time (s)
b
Extracellular spaceExtracellular space
a5Time (s)0
3
45
1 2
Extracellular
vestibule
Extracellularvestibule
54
1 2 3 4
2
3
1
Figure 7
Beta blockers bind spontaneously to the 2-adrenergic receptor (2AR) in molecular dynamics simulations, achieving thecrystallographic pose and revealing several metastable intermediate states on the binding pathway. ( a, top left) Pins indicate success
positions of a dihydroalprenolol molecule as it binds to 2AR. The ligand moves from bulk solvent (pose), into the extracellulavestibule (poses and), and finally into the binding pocket (poses and). (a, bottom) These five poses are shown in purple,the crystallographic pose in gray. (a, top right) The path taken by the ligand as it diffuses about the receptor and then binds.(b) Root mean square deviation (rmsd) of the ligand in simulation from its position in the alprenolol2AR crystal structure. Figuradapted from Reference 18.
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raise the possibility of using simulation to identify novel binding sites. Indeed, both Shan et al. and
Dror et al. discovered alternative binding sites, suggesting possibilities for the design of allosteric
drugs with improved selectivity among kinases or GPCRs.Such simulations also allow atomic-level characterization of the binding pathways andenergetic
barriers that determine binding kinetics. Dror et al. (20) found that beta blockers visit a sequenceof metastable conformations en route to the binding pocket of the 2AR (Figure 7). Surprisingly
they found that the largest energetic barrier on the binding pathway often occurs much earlier thanreceptor geometry would suggest, and appears to involve substantial dehydration that occurs as
the drug associates with a particular region on the receptor surface. Shan et al. (78) also identifiedmetastable conformations on the binding pathway, as did Buch et al. (11) in a study of an inhibitor
binding to trypsin. These studies are computationally intensive: Shan et al., Dror et al., and Buchet al. performed multiple simulations totaling over 150 s, 400s, and 50s, respectively.
A common application of proteinligand simulations is to compute the binding affinity of a
ligand, often a drug candidate,to a known binding site. Unbiased MD simulations of ligandbindingare usually ill suited for this purpose, as precise estimation of ligand affinity would typically require
seconds to hours of simulated time in order to observe sufficiently many binding and unbindingevents. Fortunately, binding affinity calculations can be performed much more efficiently using
methods such as free energy perturbation (107) or thermodynamic integration (40), which involveusing a family of modified force fields to bias a series of simulations in ways that accelerate
the forming and breaking of protein-ligand interactions. These biasing forces can be physicallyintuitive, such as forcibly pulling a ligand into or out of a known binding pocket (99), or more
abstract, such as gradually turning off all interactions between a ligand and its surroundings (38). Ifthe artificial energy functions are properly constructed, unbiased binding affinities can be efficiently
and quantitatively derived from the biased simulations.
One compelling example of simulation-based binding affinity calculations is recent work onHIV reverse transcriptase. Starting with a weakly binding ligand that displayed no activity as a
reverse transcriptase inhibitor, Zeevaart et al. (103) used Monte Carlo simulations (closely relatedto MD) to calculate the relative binding energies of a family of closely related molecules. By
selecting variants predicted to bind more tightly, they discovered several molecules that provedexperimentally active in protecting human T-cells from HIV infection.
FUTURE FRONTIERS OF BIOMOLECULAR SIMULATION
As MD simulations become faster and more accurate, they will almost certainly find applications
beyond the categories we have discussed. In this section, we speculate on other areas on whichbiomolecular simulation may make an impact in the coming years, and highlight some of the
methodological problems whose solutions may help make these possibilities reality.
Drug Design
A major goal of structural biology in general, and biomolecular simulation in particular, has long
been to assist in the design of therapeutics. Simulations are already sometimes utilized as part ofthe drug development process. Simulation-based binding affinity calculations, for example, guided
the design of HIV reverse transcriptase inhibitors mentioned above (103), as well as the subsequentdesign of inhibitors that maintain high potency in the presence of a drug-resistance mutation (37)
The use of MD in mainstream drug discovery efforts, however, remains limited.In the future, simulation may offer a number of opportunities for improving the drug discovery
process. Simulation-based methods can compute ligandprotein affinities more accurately than
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standard docking methods, aiding in the identification of lead compounds through virtual screen-
ing of drug candidates or through a fragment-based approach. Accurate evaluation of binding
affinities may prove even more useful in the subsequent process of lead optimization, or in avoid-ing toxicity by ensuring that drug candidates do not bind to known antitargets. MD also has the
potential to discover novel binding sites, including pockets that are not present in existing crystalstructures (75, 78). In addition, simulations may allow refinement of low-resolution structural
models for proteins, thus enabling structure-based drug design.MD also allows the examination of interactions between known drugs and genetic variants
of protein targets. If a disease becomes resistant to a drug, simulations of the mutated targetsmay elucidate the mechanism of resistance and facilitate modifications that restore drug efficacy
(37). Simulations might even aid in the design of drugs or drug cocktails tailored to the geneticmakeupand thus the unique protein variantsof a particular individual.
Perhaps more importantly, the insights MD can provide into the functional mechanisms of
proteins involved in disease pathways may facilitate the identification of appropriate targets andthe design of drugs that target those proteins. Many drugs may prove more effective if they bind
preferentially to a specific conformation of their target protein. Such conformational selectivitycould allow finer control of cellular signaling by stabilizing a particular conformation of a re-
ceptor, or reduce side effects by favoring binding to a protein when it is in a particular state ofactivity.
Protein Design
By facilitating optimization of properties such as structure, ligand-binding affinity, or enzymaticactivity, MD may play a role in the design of proteins for use as biosensors, industrial catalysts,
or therapeutic antibodies, among other potential applications. MD has already been used to rankcandidate amino acid sequences on the basis of calculated properties such as binding affinity (41,
59, 101). It may be used in the future not only to test whether a protein binds a ligand, forms a
desired interface with another protein, or folds correctly, but to guide the design process in orderto achieve such properties. One might even imagine a simulation during which a protein gradually
evolves, favoring mutations that improve some measure of its fitness.
Modeling Nucleic Acids
Although nucleic acids are often viewed as static structures that primarily carry sequence infor-
mation, experimental investigations of both RNAs and DNAs, such as those found in ribosomes,riboswitches, Holliday junctions, and nucleosomes, reveal diverse structures, functions, and dy-
namics. As with proteins, accurate, long-timescale simulations of nucleic acids should enrich ourunderstanding of important biological mechanisms and may help reveal promising drug targets
and binding sites.
To date, MD simulations of proteins have vastly outnumbered those of nucleic acids
(56). Reasons for this disparity include both the smaller number of available nucleic acidstructures and the relative immaturity of nucleic acid force fields. The Protein Data Bank(http://www.pdb.org/)currently contains over 75,000 solved structures, whereas the Nucleic
Acid Database(http://ndbserver.rutgers.edu/)contains just over 5,000. Force fields for nucleicacids are still undergoing extensive refinement (15, 64, 102, 104), and it is unclear whether the
high charge density of nucleic acids and commonly associated divalent cations can be accuratelysimulated using simple point charges; polarizability may be essential.
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Simulation of Complex Biological Structures
The increasing availability of high-resolution structures for biological units of greater size andcomplexitymotor proteins, multiprotein complexes, ribosomes, whole organelles, and even sim-
ple organismsoffers the opportunity to investigate their dynamics through simulation. All-atomMD simulations have already been employed to model a few such units. Using simulations of more
than two million atoms, Sanbonmatsu et al. (74) investigated howa ribosome selectstransfer RNAs(tRNAs) with high efficiency and accuracy and found that the flexibility of tRNAs was crucial to
maintaining the complementary codonanticodon geometry. Freddolino et al. (26) conductedMD simulations of the complete satellite tobacco mosaic virus, including a capsid consisting of
60 copies of a single protein and a 1,058-base RNA genome. These simulations, with more than a
million atoms, suggested that the presence of the RNA is necessary for the assembly and stabilityof the capsid.
Although such million-atom simulations are impressive, cellular organelles, let alone entirecells, are dramatically larger; a mitochondrion, for instance, is about half a micron in diameter and
comprises over ten billion atoms. Further, the functional timescales of large protein complexesand organelles tend to be substantially longer than those of individual proteins, often extending
to seconds or more. Such spatial and temporal scales are well beyond those of even the mostadvanced MD simulations. Fortunately, complex biological structures usually have a hierarchical
and modular organization; it may thus be especially productive to develop multiscale models thatuse the most appropriate abstraction and representation for each temporal and spatial scale. The
challenge lies in integrating all-atom MD simulations seamlessly into such multiscale models.
Enabling Longer and Cheaper Simulations
Although the millisecond-scale simulations recently performed on Anton represent a significantmilestone, many biochemical events take place on timescales well above a millisecond and involve
systems larger than those currently being simulated. The efficiency of a parallel MD simulationon a given hardware platform is determined roughly by the ratio of atoms to processors, so to first
approximation one can roughly maintain simulation speed for larger chemical systems by using
additional processors. In practice, however, cost often becomes a limiting factor. Accessing longertimescales is even more difficult: Because of interprocessor communication limits, one cannotincrease the speed of todays fastest simulations by simply adding more processors. Innovation
in some combination of algorithms, computer architecture, and enhanced sampling methods isthus necessary to reach longer timescales and to reduce the cost of large simulations. Such work
is currently under way, both in our group and elsewhere.
More Accurate Biochemistry
Despite recent improvements in force fields, MD simulations still face a number of sources oferror. First, force fields remain imperfect. Second, most contemporary MD simulations do not
fully capture the detailed molecular composition of biological systems, in which many differenttypes of molecules are present, both in the aqueous phase and in lipid bilayers. In the past, force
field inaccuracies often overshadowed the effects of unrealistic composition, but as force fieldsimprove, accurately modeling molecular composition will become more important.
Third, classical MD simulations treat covalent bonds as unchanging. Chemical reactions, inwhich covalent bonds break or form, are typically simulated using techniques such as quantum
mechanics/molecular mechanics simulations, in which the bulk of the system is simulated as
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in classical MD, but a small part is evaluated using more computationally intensive quantum
mechanical approaches (39, 77). Several methods are under development to handle chemical
reactions directly within an MD framework. Some of these concentrate on capturing changes tothe protonation states of ionizable amino acid residues (57, 89). Reactive force fields, which allow
covalent bonds between arbitrary pairs of atoms to break or form, have thus far been limited tosimple inorganic and organic molecules (94) but may eventually capture more general enzymatic
reactions.A promising avenue to improve the accuracy of MD simulations is to incorporate experimen-
tal data directly into the simulations. NMR data, for example, has been used to restrain MDsimulations, biasing the protein conformations toward those compatible with the experimental
measurements (48). A general framework allowing incorporation of biophysical, biochemical, andeven evolutionary data into MD simulations may prove useful both in interpreting experimental
data and in making simulation-based predictions.
SUMMARY POINTS
1. MD simulation can serve as a computational microscope, revealing the workings of
biomolecular systems at a spatial and temporal resolution that is often difficult to accessexperimentally.
2. Until recently, even the longest atomic-level MD simulations fell short of the microsec-ond and millisecond timescales on which biochemical events such as protein folding,
proteindrug interactions, and major conformational changes typically take place. Thespeed of the fastest MD simulations has increased 1,000-fold over a period of several
years, however, due to the development of specialized hardware and better paralleliza-
tion algorithms. All-atom simulations of proteins can now reach timescales in excess ofa millisecond.
3. These developments, combined with the improvements to the force field models that
underlie MD simulations, have allowed MD to capture in atomistic detail processes such
as the conformational transitions essential to protein function, the folding of proteins to
their native structures, the transport of small molecules across cell membranes, and thebinding of drugs to their targets.
FUTURE ISSUES
1. Many biochemical events still take place on long timescales that areinaccessibleto atomic-
level MD simulations, or on large spatial scales that make atomic-level simulation inor-dinately expensive. Further improvements in algorithms and computer architectures are
needed to make simulations faster and more cost-effective.
2. Multiscale models and enhanced sampling methods will likely also play an essential role
in capturing events at larger temporal and spatial scales.
3. Force fields require further improvement and validation, particularly for the modeling
of nucleic acids, certain ions, and some types of ligands.
4. Classical MD does not capture breaking and formation of covalent bonds, but it may be
possible to handle such reactive chemistry within a generalized MD framework.
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5. Application of MD simulation to the design of drugs and proteins remains fertile ground
for future research.
DISCLOSURE STATEMENT
David E. Shaw is the beneficial owner of D. E. Shaw Research and serves as its Chief Scientist.
ACKNOWLEDGMENTS
We thank Anton Arkhipov, David Borhani, Michael Eastwood, Morten Jensen, John KlepeisKresten Lindorff-Larsen, Venkatesh Mysore, Albert Pan, Stefano Piana, and Yibing Shan for
stimulatingdiscussions, helpful comments, and assistance with figures, and Mollie Kirkfor editoriaassistance.
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Annual Review o
Biophysics
Volume 41, 2012Contents
How Should We Think About the Ribosome?
Peter B. Moore 1
Mechanisms of Sec61/SecY-Mediated Protein Translocation
Across MembranesEunyong Park and Tom A. Rapoport 21
Racemic Protein CrystallographyTodd O. Yeates and Stephen B.H. Kent 41
Disulfide Bonding in Protein Biophysics
Deborah Fass 63
Prokaryotic Diacylglycerol Kinase and Undecaprenol Kinase
Wade D. van Horn and Charles R. Sanders 81
Allostery and the Monod-Wyman-Changeux Model After 50 YearsJean-Pierre Changeux 103
Protein Structure in Membrane DomainsArianna Rath and Charles M. Deber 135
Bacterial Mechanosensitive ChannelsMscS: Evolutions Solution
to Creating Sensitivity in FunctionJames H. Naismith and Ian R. Booth 157
Cooperativity in Cellular Biochemical Processes: Noise-Enhanced
Sensitivity, Fluctuating Enzyme, Bistability with Nonlinear
Feedback, and Other Mechanisms for Sigmoidal
Responses
Hong Qian 179
Network-Based Models as Tools Hinting at Nonevident
Protein FunctionalityCanan Atilgan, Osman Burak Okan, and Ali Rana Atilgan 205
Filamins in Mechanosensing and SignalingZiba Razinia, Toni Makela, Jari Ylanne, and David A. Calderwood 227
vii
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ATP Util