Biosensors Final Presentation - Fall 2015

Photonic Biosensors

Faculty AdvisorDr. Seung Kim

© Israel Flores 2016

Importance

• Hundreds of thousands of deaths from treatable diseases in each year• Early detection increases survival rates


Our Goal

• Early diagnosis the key to effectively

treating diseases like cancer.

• Low Cost

• Point of Care Diagnosis

• Portable Biosensor

To develop a Point-of-Care (POC) Biosensor for medical diagnosis to monitor health status


CD Based Microfluidics

Laura DeOtteLuke Funk


Why CD-based Microfluidics?•Optical system requires fluid to be in a

~2.5 µm cavity

•Microfluidics effectively transports fluid from input to sensing region

• CD-based design: • Space effective

• Cost effective

• Controllable fluid flow


Microfabrication: Photolithography

• Silver sputtering

• SU8 spin deposition

• UV light exposure

• SU8 developing

• Bonding


Fall 2015 Design


CD Mask Design


Channel Selection

• Fluid ‘split’ in channels with a large change in cross-section

• Fluid continuous in uniform channels • Easier to fabricate • 1 mm channel width


Results

• Capillary action capable of filling channel and sensing region

• Reduce fill time by spinning


Results• Spinning necessary to transport fluid

to waste reservoir

• Goals: • Use calibration fluid • Reusable channels


Future Plan• Disc design improvements

• Simplify microfabrication

• Easier to align sensing region

• 4 identical channels on each disk

• Functionalization of sensing region surface with bioreceptors


Optical System

Tony Bujana


Optical System


Fabry-Perot Cavity

R: reflectance of partially reflecting mirrorL: distance between the mirrorsn: refractive index inside of the cavity


Scaled Differential Calculation

ΔP1= -0.5262ΔP2= 0.39 Δη= 1.252


ResultsCamera 1. 780nm Laser

Camera 2. 850nm Laser


Future Plan (Bio-detection)


Stopping Mechanism

Nathan Strachen


Control System

• Precise positioning of disk• Closed loop control • User friendly

Rotate CD at high RPM for a specified

period of time Stop CD at a specific angle


Our Approach

• Dual-Motor system - Stepper Motor - DC Motor - Pulley Connection

• Stepper motor allows for precise positioning • DC Motor enables rotation speeds up to

4600 RPM• IR sensor is used for reference point


Implementation & Results


Enclosure System

Stephen Sumrall


Photonic Early Diagnoses

Optics

Sample

Detection

Processing


Photonic Early Diagnoses

Combine different wavelength lasers and shooting them at the sample

The brains of the operation, interpret detection results from the process

Detecting changes in wavelength

Move sample through disc and position across laser beam

Optics

Sample

Detection

Processing


Photonic Early Diagnoses Enclosure

Sample

Detection

Processing

Optics


Sample


Processing


Photonic Early Diagnoses Enclosure

Optics

Sample

Detection

Processing


Prototype I (2015) Prototype II (2016)


System Integration

Israel Flores


Up to Speed


Progress Report• New Library

• Camera Interface• Successful Data

Frame Exchange

Team Friendly Software for rapid test script development


Outlook


Budget


Gantt Chart Spring 2016

Enclosure System

Biodetection Fabrication technique

Microcomputer Stand alone system

Optics detection

Microfluidics Stage 1

Enclosure System Final Design

Biodetection

Microcomputer

Optics

Microfluidics

Final Device Complete5/19/1888 3/27/1924 2/3/1960 12/11/1995 10/18/2031


…Thank You© Israel Flores 2016

Documents

Biosensors Final Presentation - Fall 2015