Biotech

Cloning a mammal

PCR

• This is the polymerase chain reaction. It is a technique to multiply a sample of DNA many times in a short period of time. It supplies the scientist with sufficient DNA for further testing.

http://www.dnalc.org/resources/animations/pcr.html

DNA fingerprinting (RFLP analysis)

• RFLP is restriction fragment length polymorphism. This is the idea that each person’s DNA would cut up into different sized fragments. Thus the pattern produced by electrophoresis of those patterns would be unique to the person.

PROBE

• Why? To “light up/identify” specific genes

• Hybridization: complementary DNA binds to a gene and holds another molecule to “light up/identify” a specific sequence of DNA.

• Often probe is radioactive or fluorescent so when exposed to film it can be detected.

Southern Blotting• Through a series of steps, DNA that has

been separated by electrophoresis is applied to a membrane of nylon or nitrocellulose.

DNA fingerprinting analysis

• http://www.biology.arizona.edu/human_Bio/problem_sets/DNA_forensics_1/02Q.html

• Do problems 2-4 to practice the interpretation of DNA fingerprints.