Biotechnology - Plant Biotechnology · effects, and includes genetic engineering and gene manipulation to obtain transgenic plants. Plant genetic engineering is used to produce new

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Biotechnology - Plant

Biotechnology (Transgenic plants, Herbicide Resistant Plants,

Glyphosate Tolerant Plants, Sulphonylurea Tolerant

Plants, Atrazine Tolerant Plants, Phosphinothricin

Tolerant Plants, Bromoxynil Tolerant Plants, Insect

Resistant Plants, Animal Cells, Plant Cells, Tissue

Cultures, Viruses, Prokaryotes)

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Introduction

Plant biotechnology is a precise process in which scientific

techniques are used to develop molecular and cellular based

technologies to improve plant productivity, quality and health;

to improve the quality of plant products; or to prevent, reduce

or eliminate constraints to plant productivity caused by

diseases, pest organisms and environmental stresses. It can be

defined as human intervention on plant material by means of

technological instruments in order to produce permanent

effects, and includes genetic engineering and gene

manipulation to obtain transgenic plants. Plant genetic

engineering is used to produce new inheritable combinations

by introducing external DNA to plant material in an unnatural

way. The results are genetically modified plants (GMPs) or

transgenic plants.



The key instrument used in plant biotechnology is the plant

tissue culture (PTC) technique which refers to the in vitro

culture of protoplasts, cells, tissues and organs. Plant

biotechnology in use today relies on advanced technology,

which allows plant breeders to make precise genetic changes

to impart beneficial traits to plants. The application of

biotechnology in agriculture has resulted in benefits to

farmers, producers and consumers. Plant biotechnology has

helped make both insect pest control and weed management

safer and easier while safeguarding plants against disease.

The worldwide demand for food, feed and modern textile fibers

can only be met in the future with the help of plant

biotechnology. It has the potential to open up whole new

business areas that will totally redefine the current market

scope and perception.



Market Outlook

The Indian biotech industry holds about 2 per cent share

of the global biotech industry. The biotechnology industry

in India, comprising about 800 companies, is valued at

US$ 11 billion and is growing at a Compound Annual

Growth Rate (CAGR) of 20 per cent. If there is an annual

investment of US$ 4.01 billion to US$ 5.02 billion in the

next five years, the biotech industry can grow to US$ 100

billion by 2025, with a 25 per cent return on investment,

and set a growth rate of 30 per cent year-on-year.



The global market for agricultural biotechnology is

expected to grow to $46.8 billion by 2019, with a five-

year compound annual growth rate (CAGR) of 11%. The

biotechnology tools category, the fastest growing

segment of the market, is moving at phenomenal 49.9%

CAGR.



Table of Contents

1. The organisms of biotechnology

Cells - The Basic UnitsTypes of MicroorganismVirusesProkaryotesEukaryotesAlgaeProtozoaFungiTissue Cultures Animal CellsPlant Cells



2. Transgenic plants

Herbicide Resistant Plants

1. Glyphosate Tolerant Plants2. Sulphonylurea Tolerant Plants3. Atrazine Tolerant Plants4. Phosphinothricin Tolerant Plants5. Bromoxynil Tolerant Plants

Insect Resistant Plants

1. Transgenic Plants with Bt Toxin2. Transgenic Plants with Bt Toxin and Serine Protease

Inhibitor Gene



3. Transgenic Plants with Cowpea Trypsin Inhibitor4. Transgenic Plants with Nicotiana alata Proteinase Inhibitor

Virus Resistant Plants

1. Transgenic Plants with Viral Coat Protein2. Transgenic Plants with Viral Nucleoprotein3. Transgenic Plants with Viral SAT RNA4. Transgenic Plants with Antisense RNA

Transgenic Plants Resistant to Fungi and BacteriaTransgenic Plants with Improved Storage ProteinsSweet ProteinsEnriching the Carbohydrate ContentsImproving the Quality of Oils and Fats



Male Sterility and Fertility RestorationChanging the Flower ColoursStress Tolerant PlantsCold Tolerant PlantsDrought Tolerant PlantsPlant Tolerant to High Light IntensityEngineering for Preservation of FruitsEnhancing the Photosynthetic EfficiencyTransgenic Plants as BioreactorsVaccinesInterferonsPharmaceutical CompoundsBiodegradable Plastics



3. Biological Nitrogen fixations

Non-symbiotic Nitrogen FixationFeatures Favourable for Non-symbiotic Nitrogen Fixation

1. Special separation of Nitrogen Fixing Cells2. Protein-Nitrogenase Association3. High Rate of Respiration4. Time specific Nitrogenase Activity5. Association with Rapid Oxygen Consumers6. Presence of hydrogenase7. Colonization

NitrogenaseBasic requirements for Nitrogen Fixation



Mechanism of Nitrogen ReductionAssimilation of AmmoniaRoute I

Route IISymbiotic Nitrogen FixationHost SpecificityRoot NodulationMechanism of Nitrogen Fixation

(a) Oxygen Transpot by Leghaemoglobin(b) Utilization of Oxygen by Hydrogenase

NitrogenaseRequirement for Nitrogen reductionAssimilation of Ammonia



4. Genetics of Nitrogen Fixation

Nif-genes of Klebsiella PneumoniaeRegulation of Nif GenesNif-genes of AzotobacterNif-genes of AnabaenaGenetics of Legume-Rhizobium Nitrogen Fixation

1. Rhizobial Genes

a) Nod Genesb) Nif Genesc) Hup Genes



2. Legume Nodulin Genes

Leghaemoglobin GeneOverall Regulation of GenesGene Transfer for Nitrogen Fixation

1. Transfer of Nif Genes to Non-Nitrogen Fixing Bacteria2. Transfer of Nif Genes to yeasts3. Transfer of Nif-Genes to plants4. Transfer of Nod Genes5. Transfer of Hup Genes



5. Mycorrhizae for Agriculture and

Forestry

Mycorrhizal types and their structural and nutritional featuresEctomycorrhizaeMechanism of ECM formationMorphology and structureSynthesis of mycorrhizaCultural studyVesicular arbuscular MycorrhizaIntroductionEvolution



TaxonomyClassificationDistributionLifecycleReproductionSexual reproductionA sexual productionMethod of Inoculum production of VAMSome important steps in production of VAMHost plant/growth medium



Fertilizations/micronutrientsChemical applicationControl of fungal pathogensPlant vesicular arbuscular mycorrhizal fungal interactionsVAM and soil biotaControl of root diseasesEndomycorrhiza fungi and tree diseasesMechanism of disease control



6. Animal and plant cell cultures

Historical perspectivesProducts and potentialsAnimal cellsImmuno biological

1. Virus vaccines2. Monoclonal antibodies3. Immunoregulator materials

InsectisidesEnzymesHormonesWhole cells



Plant cellsPharmaceuticalsFood additivesAgrochemicalsPerfumesEnzymesSpeciality ChemicalsBiomass applications of plant cell culturesCell culture and product synthesisThe nature of animal and plant cells in cultureCell culture initiationCulture developmentSecondary culturesCulture replicationIndustrially useful cell cultures



Substrate independent culturesIndividuality of cell lines in relation to the productivityCulture mediaGrowth mediaWaterInorganic saltsTrace elementsVitaminsBuffersSources of energy and carbonNitrogen sources

1. Defined nitrogen sources2. Undefined nitrogen sources



Growth factorsOther ingredientsMaintenance mediaCell culture technologiesCellular characteristics which influence the choice of cell culture technologyMixingAerationDoubling times

1. Sterlization of media2. Sterlization of equipment

Cell sticknessImmobilized cell systems



The growth and exploitation of cell grown on the surface of a supporting solid substratum

1. Multiple process2. Unit process

The growth of animal and plant cells immobilized within a confining matrix

1. Gel entrapment systems2. Applications of entrapped cells

Dynamic cell systemsAir driven systemsImpeller and air driven systemsImpeller mixed systems



7. Somaclonal variation, cell selection

an genotype improvement

Somaclonal variationHistorical perspectiveThe manifold incidence of somaclonal variationRange of speciesCharacters displaying variationGenetic nature of somaclonal variantsPre-existing or culture induced variationGenetic and explant sources effects



The origin of somaclonal variationChromosomal abnormalitiesMolecular possibilitiesGene amplification and diminutionTranposable elementsCell selectionDisease resistanceHerbicide toleranceNutritional qualityOther cell selection systems



8. Virus-free clones through plant tissue

culture

Distribution of viruses in plantsTechniques for eradicationHeat treatmentChemotherapyMeristem cultureCulture mediaFactors affecting developments and rootingVirus eradicationMajor use of virus-free clonesStudy effect of virus infectionSource for clonal propagationSource for in vitro mass propagationConcluding remarks



9. Microbial metabolism of carbon

dioxide

Autotrophic carbon dioxide fixationThe calvin cycleMolecular structure and properties of RuBP casePhosphoribulokinaseCarboxysomesRegulation of ribulose 1,5-biphosphate carboxydase and phosphoribulakinase synthesisThe reductive carboxylic acid cycleThe anaerobic non-phototrophic autotrophsHeterotrophic carbon dioxide fixation



10. Microbial metabolism of Hydrogen

IntroductionThe role of Hydrogen in the biosphereEnzyme catalysing the evolution and oxidation of HydrogenH2 :+ Ferredoxin OxidoreductaseH2 : Ferricytochrome C3 oxidoreductaseH2 : NAD- OxidoreductaseH2 : Coenzyme F420 oxidoreductaseMembrane-bound hydrogenasesFormate hydrogenlyaseNitrogenaseOrganisms involved in the conversion of hydrogen



Hydrogen-producing micro-organismsAnaerobic conditions

1. Fermentation and fermentative bacteria2. Anoxygenic photosynthesis and phototrophic bacteria3. Oxygenic Phototrophic bacteria (Cyanobacteria)4. Oxygenic green algae

Aerobic conditions : Nitrogen fixing bacteriaHydrogen consisting organismsHydrogen utilization by anaerobes

1. Nitrate-reducing dentifying bacteria2. Sulfate reducing bacteria3. Methanogenic bacteria



4. Acetogenic bacteria5. Furmarate-reducing bacteria

Hydrogen utilization by phototrophs

1. Anoxygenic phototrophs2. Cyan bacteria3. Green algae

Hydrogen utilization by aerobic hydrogen-oxidizing bacteria The potential use of Hydrogenases and hydrogen in biotechnology



11. Microbial grwoth dynamics

Microbial growth in unlimited environmentsBasic growth equation from cell number increaseBasic growth equation from increment increase in the population over a small growth time.Basic growth equations.Microbial growth in limited environmentsGrowth limitation by substrate exhaustionVariation in the observed growth yieldInfluence of the growth-limiting substrate on growth rateDeviation of the Monod equation at High substrate concentrationsBasic growth limiting substrate equationModelling microbial growth in limited environmentsThe logistic equation



The saturation modelMicrobial growth in open environmentsChemostat growth kineticsThe dilution rateThe dilution rate and biomass concentrationThe dilution rate and growth limiting substrate concentrationBiomass and growth-limiting substrate concentrations in the steady stateDetermination of Âµmax from washout kineticsEstablishing and maintaining the steady stateDeviations from theoritical chemostat kineticsInfluence of variation in the observed growth yield Microbial competitionCompetition in closed environmentsCompetition in open environments



12. Stoichiometry of microbial growth

Growth yields and material balancesRelation between ATP production and growth yields, YATPInfluence of growth rate and maintenance energy on YATP : anaerobic chemostat culturesAerobic yield studies and the influence of the efficiency of oxidatie phosphorylation on growth yieldsTheoritical calculations on the ATP requirements for the formation of microbial biomassInfluence of Cell Composition



Influence of the carbon source and complexity of the mediumTheoritical calculations on the ATP requirement for the formation of microbial biomassInfluence of the Nitrogen sourceInfluence of the carbon assimilation pathway of the growth substrateEnergy-dissipating mechanisms during growth with excess carbon and source.Influence of the degree of reduction of the growth substrate Heat productionThe stoichiometry of product formation



13. Ageing and death in microbes

Basic principlesDeath of microbesAgeing of microbesViability among microbesSurvival of populations : Cryptic growthInjury among microbesStress and survivalThe physiological status of the populationOvert and actual stressStarvationSubstrate accelerated death (SAD)Metabolic and structural injuryThymine less deathSurvival of slowly growing bacteriaDifferentiation and survival



14. Effect of environment on microbial

activity

Mechanisms of micro-organisms response to the environment Primary response due to direct chemical or physicochemical effectsEnzyme inhibition and stimulationInduction and repression of protein synthesisChanges in cell morphologyChange in genotypeDissolved oxygenCell Interactions with oxygenRespiration Oxygen incorporationOxygen as an inhibitorOxygen as an enzyme regulator



Measurement of dissolved oxygenGeneralized response to DOTDiffusion limitationResponse of growing micro-organismsRespiration rateChange in cell constituentsChanges in metabolic productsTransient responses to changes in DOTControl of DOTRedox potentialResponses to carbon dioxideRequirement for carbon dioxideInhibition by carbon dioxideWater activityIntroduciton



Halotolerance and halophilyEffects of pHIntroductionCellular level responsesIntracellular pHEffects of pH membrane functionEffects of pH on uptake of substrateEffects of pH on products of metabolismEffects of pH on cell morphology an structureEffects of pH on the chemical environmentEffects of pH on flocculation and adhesionOptimum pH values for growthCauses of pH changes in culturesProduct formationNutrient uptakeOxidation/reduction reaction



Chage in buffering capacityControl of pHBy means of a bufferBy balancing metabolismBy feedback controlTemperatureCellular-level ResponsesTemperature ranges for growthResponse of growth rate to temperatureEffects of temperature on cell deathEffects of temperature on cellular components

1. Membranes2. DNA3. RNA4. Proteins



Cultural effects of temperatureResponse to temperature shiftsEffects on substrate utilizationEffects on product formationHeat generationShearGeneration of shearEffects of shear on filamentous fungiEffects of shear on protozoa and animal cells in cultureEffects of products on shear rateGeneral control strategies



15. Biosynthesis of fatty acids and lipids

NomenclatureRelevance and importance of lipidsLipid composition of micro-organismGeneral surveyBacteriaYeastsFungiOleaginous micro-organismPatterns of lipid accumulationFactors influencing lipid biosynthesisGrowth rateSubstrateTemperature



Growth substrateOxygenpH and salinityOther factorsLipid biosynthesisAcetyl- CoA carboxylaseFatty acid sythetaseOrigin of acetyl - CoABacteriaEukaryotic micro-organismBiosynthesis of unsaturated fatty acidsBiosynthesis of other fatty acidsBiosynthesis of lipids from fatty acidsTriacylglycerolsPhospholipids



WaxesPoly B- hydroxybutyrateMicrobial metabolism of alkanes and fatty acidsAlkane-utilizing organismsUptake of alkanesMechanisms of alkane oxidationOxidation of primary alcohols to fatty acidsMetabolism of fatty acids derived from alkanesB-oxidationa-oxidationMicrobial products derived from alkanesFatty alcohols and aldehydesFatty acidsSurfactants



16. Microbial metabolism of aromatic

compounds

Fission of the Benzene nucleusPereparation of nucleus for aerobic fissionReactions which follow ring fissionPathways of degradationMeta fission pathwaysDegradation of 4-hydroxyphenlacetic, homoproto catacleuicHomogentistic and genetoside acidsProcalecluate 4.5 dioxygenase



Degradation of 3.0- Methylgllic acid: Biological formationofmethanolOrtho fission pathwaySeparation of pathways used for aromatic catabolism by bacteriaCatabolism of aromatic compounds in trichosporon cutaneumDegradation of aromatic industrials pollutants and pesticides Complete mineralizationCatabolic plasmidsRelease of halogen substrates from benzen nucleusIncomplete degradation of aromatics



17. Bacterial respiration

The generation of the proton motive forceBacterial respiratory chainsRespiration linked proton translocationThe proton motive forceThe utilization of the proton motive forceATP synthesisActive transport of solutesBiotechnological aspects of bacterial respirationBiomass productionWaste treatment and metabolite production



18. Mechanisms of enzyme catalysis

The events in an enzyme catalysed reactionEnzyme mechanismsEnzyme kineticsBinding of the substrate to the enzymeConformational changesCovalent bond making and breakingGlucose isomerase



19. Enzyme evolution

Regulation of metabolismInductionNutritional repressionFeedback regulationLimiting accumulation of end productsFeedback resistance mutationsAdditional types of regulationsPermeability considerationRecent approaches to strain constructionAmino-acid production by genetically engineered strains of E-Coli and related organismsStrain construction in other species



20. Microbial photosynthesis

Historical backgroundGeneral characteristics of microbial photosynthesisStructure and synthesis of photosynthetic pigmentsChlorophylls and bacteriochlorophyllsCarotenoidsThe phycobissinsThe initial reactions primary photochemistry and electron transportLight harvestingCharge separation and electron transport in an oxygenic photosynthesisATP synthesis



The eubacterial photosynthetic microbesIntroductionThe anoxygenic phototrophic bacteriaThe major groupsDevelopment of the photosynthetic appartusCarbon metabolismThe Cyano bacteria: oxygenic photosynthesis in a diverseprokaryotic groupOrganization of the photosynthetic appartusInterrelationship between photosynthetic and chemosynthetic carbon metabolism in cyanobacteria



21. Extra cellular enzymes

Mechanism of SecretionSignal hypothesisSignal hyprosthesis in bacteriaSignal sequence structureFunction of signal peptide and translocationProcessing of the precursorGene fusion studiesMembrane associate intermediatesAlternative export mechanisms; post translocational secretionAspects of enzyme secretion in fungi



Regulation of Extracellular enzyme synthesisRegulation of protein synthesisInduction of exoenymesEnd-product repressionCatabolite repressionPatterns of exoenzyme synthesisRNA polymerase modificationCatabolite repressionTranslocational control of exoenzyme synthesis in bacteriaControl of secretion



22. Overproduction of microbial

metabolites

Effects of nutrient limitationEffects of pH and uncouplers of oxidative phosphorylationEffects of Temperature



23. Regulation of metabolite synthesis

A phospholactase system in KlebsiellaCatabolism of unnatural sugarsRegulatory mutationsModular pathwaysEvolution of an aliphatic amidase in pseudomonasEvolution of a new ÃŸ-Galactosidase in E-ColiProperties of the wild-type proteinsEvolution of lactose utilizationEvolution of new activities for ebg enzymesEvolution of the ebg repressorDecryptifying Existing Genes


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Nif-Genes of Azotobacter, Nif-Genes of Anabaena, Legume Nodulin Genes, Transfer of

Nif Genes to Yeasts, Transfer of Nif-Genes to Plants, Transfer of Hup Genes,

Mechanism of ECM Formation, Method of Inoculum Production of VAM, Animal and

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Niir Project Consultancy Services (NPCS) can provide Process Technology Book on

Biotechnology - Plant Biotechnology (Transgenic plants, Herbicide Resistant Plants,

Glyphosate Tolerant Plants, Sulphonylurea Tolerant

Plants, Atrazine Tolerant Plants, Phosphinothricin

Tolerant Plants, Bromoxynil Tolerant Plants, Insect

Resistant Plants, Animal Cells, Plant Cells, Tissue

Cultures, Viruses, Prokaryotes)

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Documents

Biotechnology - Plant Biotechnology · effects, and includes genetic engineering and gene manipulation to obtain transgenic plants. Plant genetic engineering is used to produce new