BLACK SEA ECOSYSTEM-AN IMPORTANT TARGET AND SOURCE … · BLACK SEA ECOSYSTEM-AN IMPORTANT TARGET AND SOURCE OF BIOCHEMICAL COMPOUNDS AND BIOPRODUCTS PhD biochem. Daniela Mariana

31.05 - 2.06.2016

STANDArD-Murighiol, Romania

BLACK SEA ECOSYSTEM-AN IMPORTANT TARGET AND

SOURCE OF BIOCHEMICAL COMPOUNDS AND

BIOPRODUCTS

PhD biochem. Daniela Mariana Rosioru

Head of Marine Biochemistry Laboratory

National Institute for Marine Research and Development “Grigore Antipa”,

300 Mamaia Blvd., RO-900581, Constanţa, Romania.

E-mail: [email protected]

Marine biotechnology concerns the environment and marine organisms

evaluation, starting with their cultivation until their processing in order to

obtain useful bioproducts in various branches.

Based on the rich tradition and background in marine biochemistry, the

National Institute for Marine Research and Development (NIMRD)“Grigore

Antipa” Constanta, Romania, has developed in time many research

directions for the exploitation of marine living resources, which included the

main marine organisms from the Romanian Black Sea area.

The utilization of marine resources in the medical-pharmaceutical domain as

a result of originality of the methods for proceeding the raw material and the

good quality of obtained bioproducts already requested on the market,

represents one of the NIMRD concerns in this field.

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First applied biochemistry research in marine field

was started by GAVRILESCU in 1950:

-determination of vitamin D in oil extract from shark

liver, in order to know the antirachitic properties

-capitalization of fish fat for pharmaceutical purposes

Between 1969 and 1970 TELEMBICI et al. researched

the content in ascorbic acid (vitamin C), glucose and

glycogen in mollusks.

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Since 1970, the concerns of biochemistry have led to

investigations on the identification, isolation and

characterization of active biological products from marine

organisms in the Black Sea.

•alginic acid and mannitol content in Cystoseira barbata,

proteolytic activity in mollusks (MARZA, 1972, 1976),

•elaboration of a process for obtaining sodium alginate from

seaweed (BOGHICI and STATE 1972),

•1971-1981- analysis of lipid fractions from mussels, Mya

arenaria and decapod crustaceans (MOLNAR, 1974; ROSOIU,

1975; MARZA et SERBAN, 1981)

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•determining of sulphomucopolysaccharide in mollusks (MOLNAR

et al.1974,1976)

•determining glycogen and amino acids in Mytilus galloprovincialis

(MARZA, 1972; MARZA et al.1976, 1981,1989)

•determining of amino acids and protein content in unicellular algae

(VOINESCU, 1976)

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Purification and biochemical characterization in terms of enzymatic

extracts from marine invertebrates and fish:

-amylase (ROSOIU, 1976-1978)

-glucosidase (MARZA si SERBAN, 1981)

-cellulase-hemicellulase (MARZA et al. 1977, 1981, MARZA 1979-1980)

-carbohydrases (MARZA si SERBAN, 1978)

- developing a process for extraction of amylase from mussel

hepatopancreas (IORDACHESCU et. al 1975, 1977)

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-study of the physical, chemical and pharmacological properties of

some products excreted by organisms in seawater (MARZA, 1988)

-obtaining of the natural inhibitors of pepsin, trypsin and chymotrypsin

from Rapana venosa (ROSOIU, 1981-1990)

-isolation, extraction and purification of hyaluronidase from the viscera

and gonads of marine fish followed by developing a method for

obtaining the substances with an anti-hyaluronidase action,

anti-rheumatic and anti-inflammatory properties known as a

pharmaceutical product ALFLUTOP (ROSOIU, 1981-1990)

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- study of active principles in celenterate species (Mnemiopsis leidyi

and Aurelia aurita), in the crustacean Artemia salina from Techirghiol

Lake, in fish species Trygon pastinaca (CRASMARU et al., 1994, 1997)

and influence on the human body

TOMA et al. (1996), ZANOAGA et al. (1996) studied the content of

albumin, globulin and glutelin in mollusks flesh.

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Within the BIOTECH project “Biotechnological methods for utilisation of

some marine resources used in medical and industrial fields“ NIMRD

analysed the BLACK SEA offer - sea water and marine organisms to

process them as REMEDIES, ADJUVANTS, NUTRITIVE SUPPLEMENTS.

( S.C. LABORATOARELE MEDICA S.R.L. )

REMEDIES - represent resources and methods able to act under the most

favourable circumstances on living organisms in order to heal or prevent

an illness.

NUTRITIVE SUPPLEMENTS and COMPLEMENTARS - are the concentrated

sources of nutritive or other substances with nutritive and physiological

effects, alone or in combination

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The SEA WATER presence in natural sanogenic practices it is not a

novelty, as it is well known its healing, anti-inflammatory and depurative

effects in heliomarine cures.

Nasal washable solutions – instillation:

• NASOMER - marine solution, aseptic filtrate, with rectified pH;

• NASOMER PROP - marine solution, aseptic filtrate, with rectified pH and

propolis;

• NASOMER FORTE - marine solution, aseptic filtrate, with rectified pH

and Echinacea extract;

• THINNING AGENTS and DEPURATIVE for nasal mucous membrane for

allergic rhinitis, acute and chronic rhino-sinusitis.

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All products are recommended for mouth cavity affections, aphta (ulcer in

the mouth), pharynges - amygdalitis, gingivitis - stomatitis, acute on

chronic.

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Gargles

•APIMER - marine solution with propolis tincture;

•APIMER FORTE - marine solution with propolis and Echinacea tincture.

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MARINE ALGAE-organic deposits, containing marine macroalgae on

beaches, after storms.

ALGE MARINE- for medical

treatment of lung, heart, thyroid

gland, circulatory diseases,

physical anaemia and physic

asthenia.

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MARINE EPIBIOSIS- represents a life community formed by organisms

which are fixed on submerged alive or not alive structures (mollusks ,

shellfish, algae )

BIOMER, EPIBIOZA MARINA- for recovery, extended intellectual and

physical effort, prevention and control of the aging phenomena.

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MUSSELS (Mytilus galloprovincialis)

RHEUMAMER-protects the

conjunctive tissues, having, in

the same time, anti-

inflammatory properties.

Ions of calcium, magnesium

and iron have were extracted

from marine mollusk shells

and fixed as chloride, lactate,

levulinate and, together with

other bioactive components,

embedded in a food

supplement called RĂDUCU,

useful in the treatment of

anemia, rickets and tetanus

related to lack of calcium.

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MARINE FISH

Marine resources like goby (Gobius miger) or hanus (Neobius

cephalanga - nostomus) are common species along Romanian

seaside of the Black Sea, with recognised nutritive value.

ARTHROMER-adjuvant in arthrosis, spondylosis, rheumatic polyartrits

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SHARK CARTILAGE

CARTILAJ DE RECHIN-arthritis,

diabetic retinopathy, adjuvant in

chemotherapy, osteoporosis,

immunosuppression, psoriasis

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BIOTECH project

“Biotechnology for new derma-cosmetic products based on peptide-

protein complex from marine resources which are unused for food chain”

• obtaining of derma- cosmetic products from roe and viscera of fish from

the Black Sea and unusable raw materials in the food industry.

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The intermediate products obtained in the research project are of two

categories:

- DERMOMAR 1 (peptide - protein complex)

- DERMOMAR 2 (lipid-protein complex),

• further serve to diversify the range of ointments by introducing

components and additives at user choice and availability.

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The research on the biotechnological

exploitation of some marine resources from

Romanian Black Sea Coast

Project director: PhD biochem. Daniela Mariana Rosioru

NIMRD - ”NUCLEU” PROGRAM - ONGOING PROJECT

“Ovidius” University Constanta

• Recovery of seaweed biomass in the Black Sea to obtain new nano - bio- pharmaceutical formula of innovative therapies for diseases of the soft tissues of the oral- facial system

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Patents for pharmaceuticals

• PRODUS HEPATOPROTECTOR, PRODUS HIPOCOLESTEROLEMIANT OSIM 80321

• AMILAZE OSIM 68328/75140

• CELULAZE HEMICELULARE OSIM 65398-70071

• MEDICAMENT ANTIULCEROS OSIM 82876/90968/96784

• COMPLEX ENZIMATIC PROTEOLITIC OSIM 98420

• HIALURONIDAZA SI MEDICAMENT TROMBOLITIC OSIM 86312/90969/102703

• COMPLEX CU ACTIVITATE ANTIHIALURONIDAZICA SI MEDICAMENTE CONDITIONATE GAMA ALFLUTOP-INJECTABIL, UZ EXTERN, APA DE GURA OSIM 82273/92641/98316/106239/108165

• PRODUS ANTISCLEROTIC OSIM 8023

• ULEIURI FARMACEUTICE OSIM 88044/166333

Monografia SUBSTANTE BIOLOGIC ACTIVE DIN ORGANISME MARINE,1992 N.Rosoiu, M.Serban

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FURTHER?

the permanent evaluation of the availability and free access to raw

natural material from Black Sea

the sustainable utilization of the marine offer

the developing of the informational and organizational

infrastructure in order to improve an accurate connection man –

ecosphere

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Documents

BLACK SEA ECOSYSTEM-AN IMPORTANT TARGET AND SOURCE … · BLACK SEA ECOSYSTEM-AN IMPORTANT TARGET AND SOURCE OF BIOCHEMICAL COMPOUNDS AND BIOPRODUCTS PhD biochem. Daniela Mariana