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1- Bosela Michael J., Gurpreet S. Smagh., and Charles H. Michle. Genetic transformation of black walnut

(Juglans Nigra). Proceedings of the 6th Walnut Council Research Symposium

2- Euch C. E., Jay-Allemand C., Pastuglia1 M.; Doumas P. Expression of antisense chalcone synthase

RNA in transgenic hybrid walnut microcuttings. Effect on flavonoid content and rooting ability. 1998,

Plant molecular biology 38: 467479

3- Lesilie C Urastsu., Lori Martin., McGranahan Gale H. Agrobacterium protocols., walnut (Juglans).

Volume 2. Chapter 27. 297-307. 2006.

4- McGranahan G.H., Leslie C Uratsu., Lori Martin., and Dandekar. A.M.Agrobacterium-mediated

transformation of walnut somatic embryos and regeneration of transgenic Plants.Biotechnology. 1988

5- Tang H. and Ren, Z. Regeneration of transgenic plants from walnut somatics embryos mediated whit

Agrobacterium tumefaciens. Acta Hort. 544, 2001.6- Zurbriggen Matas., Tognetti Vanesa B, and Carrillo Nstor. Cyanobacterial Flavodoxin Provides

Multiple Stress Tolerance. 2007

Abstract

Agrobacterium- mediated transformation was used to produce transgenic walnut (Juglans regia L.)

embryos. in this research Agrobacterium strains LBA 4404 , C58 , EHA101 carrying vectors PBI121 with gus

and nptII genes under CaMV35S and NOS promoters respectively, and P6u-ubi-FVTI included Fldand hpt

genes under the same promoters were employed. Somatic embryos were used as explant in transformation trials.

Explants were co-cultivated with recombinant plasmid bearing Agrobacterium and Inoculated embryos were

evaluated on selective media. Positive gus assay analysis with the alive embryos inoculated by LBA 4404

included PBI121 on selective media indicated the success of our transformation approach. Inoculated embryos

by LBA 4404 included P6u-ubi-FVTI carrying fldgene had higher growth related to their controls and did not

show browning and necrosis symptoms on selective media after 1 month. This is the first report on the

probability of walnut transformation byA. grobactreum-mediated in Iran that can be used for introduceing other

interested genes.

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