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1- Bosela Michael J., Gurpreet S. Smagh., and Charles H. Michle.
Genetic transformation of black walnut
(Juglans Nigra). Proceedings of the 6th Walnut Council Research
Symposium
2- Euch C. E., Jay-Allemand C., Pastuglia1 M.; Doumas P.
Expression of antisense chalcone synthase
RNA in transgenic hybrid walnut microcuttings. Effect on
flavonoid content and rooting ability. 1998,
Plant molecular biology 38: 467479
3- Lesilie C Urastsu., Lori Martin., McGranahan Gale H.
Agrobacterium protocols., walnut (Juglans).
Volume 2. Chapter 27. 297-307. 2006.
4- McGranahan G.H., Leslie C Uratsu., Lori Martin., and
Dandekar. A.M.Agrobacterium-mediated
transformation of walnut somatic embryos and regeneration of
transgenic Plants.Biotechnology. 1988
5- Tang H. and Ren, Z. Regeneration of transgenic plants from
walnut somatics embryos mediated whit
Agrobacterium tumefaciens. Acta Hort. 544, 2001.6- Zurbriggen
Matas., Tognetti Vanesa B, and Carrillo Nstor. Cyanobacterial
Flavodoxin Provides
Multiple Stress Tolerance. 2007
Abstract
Agrobacterium- mediated transformation was used to produce
transgenic walnut (Juglans regia L.)
embryos. in this research Agrobacterium strains LBA 4404 , C58 ,
EHA101 carrying vectors PBI121 with gus
and nptII genes under CaMV35S and NOS promoters respectively,
and P6u-ubi-FVTI included Fldand hpt
genes under the same promoters were employed. Somatic embryos
were used as explant in transformation trials.
Explants were co-cultivated with recombinant plasmid bearing
Agrobacterium and Inoculated embryos were
evaluated on selective media. Positive gus assay analysis with
the alive embryos inoculated by LBA 4404
included PBI121 on selective media indicated the success of our
transformation approach. Inoculated embryos
by LBA 4404 included P6u-ubi-FVTI carrying fldgene had higher
growth related to their controls and did not
show browning and necrosis symptoms on selective media after 1
month. This is the first report on the
probability of walnut transformation byA. grobactreum-mediated
in Iran that can be used for introduceing other
interested genes.
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