Nocturnin, the target fora more slender silhouette
DescriptionExopolysaccharide (EPS) produced by a planktonic
microorganism through biotechnology. nocturshape™ blue ingredient
takes advantage of the circadian rhythms and targets nocturnin in
adipocytes, a protein involved in adipogenesis and lipid
accumulation. It reduces thigh contour and smoothes skin relief,
providing a general improvement of cellulite appearance.
AppearanceOpaque gel containing 0.5% active ingredient.
INCIWater (Aqua), Propanediol, Disodium Phosphate, Xanthan Gum,
Plankton Extract, Sodium Phosphate,Glyceryl Caprylate.Preservative
free.
Propertiesnocturshape™ blue ingredient provides a slimming
effect and smooths the skin relief due to a decrease of fat nodules
at night. It also helps to reinforce the dermal matrix, enhancing
the firmness of the skin. The overall appearance of cellulite is
improved.
Applicationsnocturshape™ blue ingredient can be incorporated
into body care formulations with global anti-cellulite, slimming
and firming properties, being particularly active during the
night.
ScienceThe most relevant biological cycles are circadian
rhythms, which comprise oscillations every 24 hours. The
intracellular biological clock is controlled by a network of
feedback loops composed of activators and repressors. The main
clock genes are CLOCK and BMAL1, regulating the expression of
hundreds of downstream circadian genes. Among these genes,
nocturnin shows a rhythmic transcription pattern with a peak by
early night and it is greatly expressed in adipocytes. The protein
nocturnin promotes adipogenesis and fat accumulation. nocturshape™
blue ingredient is a biotechnological active produced by a
planktonic microorganism (Halomonas sp.) isolated in Fuente de
Piedra Lagoon (Málaga, Spain). Its shallow waters, with a high salt
concentration, determines the presence of abundant microorganisms
and constitutes a strategic point for migrating birds, especially
pink flamingos. This EPS reduces nocturnin levels and thus lipid
accumulation specifically at nighttime. Besides providing a
slimming effect, it strengthens the dermal matrix through type I
collagen synthesis, helping to reduce visible fat nodules
characteristic of cellulite condition.
Dosage 2-4%
SolubilityWater soluble.
Chronobiology for lipolysis activation
Overall cellulite improvement in 15 days
Slimming effect at nighttime
www.lipotec.com blue biotechnology
Fuente de PiedraSpain
nocturshape™blue ingredient
anti-cellulite slimming
In vitro efficacy
In vivo efficacy
1. REDUCTION OF NOCTURNIN PROTEIN LEVELSHuman subcutaneous
preadipocytes were synchronized to a day-night rhythm during the
differentiation. Cells were treated with 0.1 mg/mL nocturshape™
blue ingredient concentrate for 6 h under induced night conditions.
Levels of nocturnin were quantified by an ELISA.
2. INDUCTION OF LIPOLYSIS AT NIGHTThe same type of cells were
synchronized again during the last days of differentiation and
treated with 0.1 or 1 mg/mL nocturshape™ blue ingredient
concentrate. Glycerol release was evaluated using a Free Glycerol
Assay kit. Differentiated cells were used as control.
3. DECREASE OF LIPID ACCUMULATIONNile Red staining was used for
the quantification, by fluorescence, of lipids in human
subcutaneous preadipocytes, either non-treated or treated with
nocturshape™ blue ingredient concentrate.
Lipolysis is stimulated in adipocytes at nightIn a
dose-dependent manner, nocturshape™ blue ingredient increased
lipolysis by 23.6% at 0.1 mg/mL.
nocturshape™ blue ingredient is more effective than caffeine in
reducing lipid accumulation
The circadian protein nocturnin is reduced with nocturshape™
blue ingredient
Great visible improvement of cellulite condition in 15
daysAlmost -1 cm was obtained in just 15 days with nocturshape™
blue ingredient, achieving a reduction of -1.5 cm at the end of the
study.
nocturshape™ blue ingredient increased skin firmness by 23%The
high improvement in skin firmness is a perfect complementary
benefit making cellulite less visible.
Isaac Peral 17 Pol. Industrial Camí Ral - 08850 Gavà (Barcelona)
Spain - Tel +34 93 638 80 00 - [email protected]
The trademarks referenced in this brochure are owned by The
Lubrizol Corporation. While the claims and supporting data provided
in this publication are believed to be reliable and they are
presented free and for guidance only, there are no warranties of
any kind. All express and implied warranties are disclaimed. The
recipient is solely responsible for ensuring that products marketed
to consumers comply with all relevant laws and regulation. LIPOTEC
is the exclusive holder of the both industrial and intellectual
property rights identified herein. Recipient of this publication
agrees to indemnify and hold harmless each entity of the LIPOTEC
organization for any and all regulatory action arising from
recipient’s use of any claims or information in this publication,
including, but not limited to, use in advertising and finished
product label claims, and not present this publication as evidence
of finished product claim substantiation to any regulatory
authority.
Ed. 14C
© 2014 The Lubrizol Corporation.All Rights Reserved.
120
100
80
60
40
20
0Lip
id a
ccu
mu
lati
on
(%
)
Differentiatedcells
Caffeine(0.2 mg/mL)
nocturshapeTM
blue ingredient concentrate
(0.01 mg/mL)
nocturshapeTM
blue ingredient concentrate(0.1 mg/mL)
Non-differentiatedcells
-29.2% -22.3% -37.4%
-22.3%
120
100
80
60
40
20
0
No
ctu
rnin
leve
ls (
%)
nocturshapeTM
blue ingredient concentrate(0.1 mg/mL)
Night
20 female volunteers (25-47 years old) applied a placebo cream
or a cream with 4% nocturshape™ blue ingredient for 30 days, only
once at night.
IMPROVEMENT OF CELLULITE APPEARANCE
• Reduction of contours and skin relief Digital photographs were
used to analyse the reductions of thigh circumference, obtained
using an image analysis software, and depth-coded maps to evaluate
the surface relief.
• Assessment of skin firmnessSkin firmness was measured through
the parameter Uf by cutometry on thighs.
4% nocturshapeTM
blue ingredientPlacebo
15 days
0 days
30 days
