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introduction Botanical name :Pisum sativum L. Family:Leguminosae Chromosome no: 2n=2x=14 (diploid) Origin: Ethopia (Blixt 1970) Short day plant Self pollination Pea protein is moderately deficient in the sulphur containing amino acids methionine and cysteine The quality of peas is ascertained by tendrometer. Histones presences is responsible for mosaic resistance.
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BREEDING IN PEA AND BEANS
PRESENTATION ON BREEDINGIN PEA AND BEANS SPEAKER NISHA CHANDEL
Deptt. Of Vegetable Science, CoA, Raipur introduction Botanical
name :Pisum sativum L. Family:Leguminosae
Chromosome no: 2n=2x=14 (diploid) Origin: Ethopia (Blixt 1970)
Short day plant Self pollination Pea protein is moderately
deficient in the sulphur containing amino acids methionine and
cysteine The quality of peas is ascertained by tendrometer.
Histonespresences is responsible for mosaic resistance. BOTANY Root
:-It develops tap root system and made up one main branch and other
sub branches. Leaves :-Leaves are pinnately compound with two to
several leaflets. Stem :-The plant may be single stemmed or many
auxiliary stems may originate at the cotyledonary node or any
superior node. Inflorescence :-The inflorescence is raceme arising
from the axil of leaf. Floral biology The flower are typical
papilionaceous with green calyx comprising of five united sepals,
five petals (one standard, two wings and two keels). The stamens
are in diadelphous(9+1)condition. In peas inflorescence is a
raceme. The flowers are borne solitary or in pairs or in cluster on
elongated peduncles arising in the axil of leaves. Pea is strictly
self-pollinated in nature. Stigma is receptive to pollen from
several days prior to anthesis until one day. . FLORAL IMAGES OF
PEA Time of anthesis The anthesis observed maximum between 9:00 AM
to 12:00 Noon. The anther dehiscence starts before opening of
flower. At 16C temperature the stigma remained receptive for 3
days. After emasculation at 20-24C retained good receptivity for
only one day temperature is the factor, which affect the duration
of receptivity. cytogenetic The chromosome number of pea is
2n=14(Das and kalloo 1970). Pisumsativum includes the following
sub-species (Simmonds,1979) Pisum sativum Linn.var. hortense :
Garden pea Pisum sativum Linn.var. arvense : Field pea Pisum
sativum Linn.var. macrocarpum : Edible podded pea Pisum sativum
elatius : Wild form Pisumsativum sysiacum : Wild form Genetic
resources ICARDA (International Centre for Agricultural Research in
Dry Area) -6129 NBPGR (National Bureau of Plant Genetic Resources
-3070 IPGR(International Plant Genetic Resources) -1589 RCA
(Institute for Agrobotany) Hungry -1199 Selection indices Improved
yield potential and stability.
Standing ability and harvestability. High shelling percentage
Suitable for freezing and canning Resistant/tolerant to frost and
drought, particularly at podding. Pod shatter resistance Resistant
to disease, namely Downey mildewPowdery mildew, Rust , Wilt
Resistant to insect ,Leaf minor ,Aphids ,Pod borer ,Pea stem fly
Inheritance of qualitative characters
Plant height High heritability, over dominance ,partial dominance,
high genetic advance Days to flowering Non-additive gene action,
partial dominance, over dominance Earliness Dominant genes, High
heritability Late flowering Recessive genes, high heritability
Number of pods per plants High heritability, epistatic gene action
. Pod yield Low heritability Breeding method Introduction -
Bonneville (USA),Arkel (France), Early budger, Lincoln, Meteor
Selection-Arka ajit, Asauji,Pant upkar,
Hybridization-Jawaharmatar-1-T-19*Greater progress
Jawaharmatar-2-Russian-2*Greater progress Jawahar matar-3-T-3*Early
budger Jawaharmatar-4-T-19*Little marvel Aseem-Pusa phalguni*EC
21622 BIOTECNOLOGY Laucou et al (1998) constructed a genetic
linkage map of Pisum sativum L. Based primarily on RAPD markers
that were carefully selected for their reproducibility and scored
in popultion of 139 recombinant inbred lines. The mapping
population was derived fromfrom a cross between a protein -rich-dry
seed cultivar Terese and an increased branching mutant (k586)
obtained from the pea cultivarTorsdag. Genes for which linked
markers have been reported in the pea are listed in table TRAIT
GENE MARKER Cotyledon shape rb Vc-5 (RFLP) Fussarium wilt
resistances Fw H19.Y14,Y15(RAPD) Powedry mildew resistances Er-2
3AFLP PRIMERS Biotic AND ABIOTIC stress of pea
Powdery mildew (Erysiphe polygoni var;pisi) Jawahar peas-83, JP-71,
JP-4 Fusarium wilt(Fusarium oxysporum) Early badger, early giant,
pusa vipasha, super alaska Rust (Uromyces viciiae fabae) JP batri
brown3, JP batri brown4 Root rot Bristol Wilt Cascadia, Leaf miner
JP-179,JP-169,LMR-4,LMR-10,LMR-20 Bruches JP-9, JP-179, Leaf roll
Dakota Heat tolerance variety Lincoln , Queen of heart Herbicide
tolerant variety S.No. Name of variety Organization Breeding
Method
Specific characteristics Early variety 1. Arkel France Introduction
Suitable for fresh market and dehydration. The variety is highly
susceptible to collar rot 2. JawaharMatar 4 JNKV Jabalpur
Hybridization (T 19 and Little Marvel) Yield- 60q/ha. Mature seeds
are green and wrinkle with 18g of 100 seed weight. 3. Early Badger
USA It is a good canning variety and resistant to Fusarium wilt and
tolerant heat and drought. 4. Little Marvel England Introduction
(Chelsea Gem x Suttons Alaska) A dwarf, wrinkled seeded variety .
6. Jawahar Peas 54 (JP 54 ) JNKV Jabalpur (Arkel JM5) ('4bc' JP
501) Powdery mildew resistant variety Mid season varieties 7.
Bonneville IARI New Delhi Introduction It is susceptible to powdery
mildew disease. 8. Alderman It is an excellent cultivar for
freezing purpose. 9. Lincoln IARI Research Station Katrian It is a
good canning purpose variety. 10 Azad P-2 Kalyanpur Hybridization
cross (Bonneville 6587) A powdery mildew resistant variety 11
Sylvia Sweden Selection First blossom appears at 14th to 16th node
after 60 days of sowing beans French bean Cow pea Lima bean Field
bean Broad bean Cluster bean FRENCH BEAN Scientific name
:-Phaseolus vulgaris Family :-Leguminoceae
Chromosome No.:- 22 Origin-Southern Mexico and Central
America(Kaplan1981) Progenitor -Phaseolus aborigineus Types of
pollination:- Self pollination Taxonomy French bean belongs to the
family leguminosae under Phaseolus genus and vulgaris species. The
genus Phaseolus possesses four cultivated species, viz: 1.
Phaseolus vulgaris : The common, haricot, snap bean 2. P. coccineus
: The runner or scarlet bean 3. P. acutifolius var. latifolius :
The tepary bean 4. P. lunatus : The lima, sieve, butter of
Medagascar bean. Phaseolus vulgaris Phaseolus coccineus Plant
genetics resources
IPGR (International Plant Genetic Centre) 3220 RCA(Institute for
Agrobotany) Hungry 4350 CIAT 35891 ISAR 2075 Inheritance of
qualitative characters
S.No. Character Number of gene Types of gene action 1. Growth habit
Monogenic Indeterminate or climbing habit dominant to determinate
habit 2. Pod stringness Stringiness dominant to stringlessness 3.
Pod surface Monogenic (accacc) Shiny node recessive to non shiny 4.
Earliness Monogenic (fdfd) Early flowering dominat to late 5.
Groove of pods Non-grooved dominant to grooved 6. Pod shape
Polygenic Normal round has a general dominace over other shapes 7.
Colour of foliage Digenic Green dominant to variegated, duplicate
gene action 8. Hard seed Soft seed dominant to hard seed
Inheritance Of Resistance To Disease
Powedry Mildew A Singal Dominant Gene Bean Rust Resistance Dominant
or Incompletely Dominant Anthracnose A single dominant gene ,are
also mex-2 and mex-3 genes Bean Virus Single Recessive Gene,Mo Ri
for Bean Virus-1 Common Bean Mosaic One Dominant Gene,I Recessive
Race Specific Genes,Bc1,Bc2.Bc3 Curly Top Virus Two Epistatic
Dominant Genes Selection indices High pod yield
Nonstringy, long pods, flat or round in shape Early pod harvesting
Bush/pole plant type High number of green pods/plant High number of
pod clusters/plant High number of primary branches/plant Free from
interlocular space Photo insensitivity Wider adaptability
Resistance to disease Resistance to insects Breeding method
Introduction- Pole Type :- Kentucky Wonder, Lakshmi, Pusa himlata,
Swarna lata, SVM-1, TKD-1, Bush Type :- Bountiful, Contender,
Gaintstringless, Pant Anupama, Premier, Selection-Pant Anupama,
Phulesuyash (GK-7), Swarnapriya (CH-812) Hybridization- Arka
subidha(IIHR 909) Mutation Pusa Parvati Biotecnology RAPD markers
have been used in common beans. RAPD markers linked to disease
resistances in common bean are given in table(Kelly and Miklas
1998) Resistance gene Pathogen RAPD markers Size(bp) Co-1
Anthacnose OF 10 530 Co-2 OQ4 B355 OH20 1440 1000 450 BC-3 BCMV
OAD19 690 Mp-1 Macrophomina B386 900 Ur-3 Rust OK14 620 Ur-9 OJ13
1800 Biotic AND ABIOTIC stress of French bean
Powdery mildew Contender, Long kidney,Top crop Bean rust Kentucky
Wonder,VL Boni -1 Common Bean Mosaic Refugee, Robust Anthracnose
Cornell49-242 Stem Fly Sel-2,SVM-1, Sel-9, Sel- 4 Aphid Bruna Leaf
hopper London horticultural, contender, top crop Heat and drought
Red Mexican VARIETY S.No. Variety Developing institutions Breeding
method
Special features 1 ArkaKomal IIHR Introduction Good transport and
keeping quality, susceptible to beancommon mosaic virus,yield
potential 80q/hac. 2. Arka Subidha(IIHR 909) Hybridization (Blue
crop Contender) Plants bushy and photosensitive. . 3. Contender
IARI New Delhi It isuscepatible to bean common mosaic virus y 4.
Pusa Parvati Developed through irradiation followed by selection
from wax podded variety EC 1906. Resistant to mosaic and powdery
mildew. VARIETY 5 Ooty-1 Tamil Nadu Agricultural University
Moderately resistant to leaf spot, anthracnose and pod borer.. 6
Pant Anupama (UPF 191) GBPUA&T, Pantnagar Selection from line
UPF181 Moderately resistant to bean mosaic and angular leaf spot. 7
PhuleSuyash (GK-7) MPKV, Rahuri Selection Plant are highly tolerant
to wilt and bean mosaic virus . 8 SwarnaPriya (CH-812) CHES, Ranchi
Pure line selection Good cooking quality, suitable for daal
preparation COW PEA S.N.-Vigna unguiculata (L.) Walp Family
Leguminosea 2n=2x=22
Origin-Africa(Simmonds 1976) Pollination-Self pollination Taxonomy
These are three cultivated and two wild subspecies of cow pea these
are as follows Sesquipedalis Cultivated species
Unguiculata-Cultivated species Cylindrical-Wild species
Dekindtiana- Wild species Mensensis- Wild species FLORAL BIOLOGY
Infloresences is anunbranched axillary raceme bearing
severalflowers at the terminal end of peduncles. Cow pea flowers
are large and showy. Flowers open only 7 and 9 A.M. On cloudy days
the flowers may open even in the afternoon. Due to dehiscence
taking place beforethe opening of flower ,the cow pea is strictly
self polllinated in nature. Selection indices High Green Pod Yield
High Seed Yield Earlieness
Wider Adaptability Appropriate Plant Type Short Tender Pods For
Whole Pod Processing Long Tender And Stringless Pods For Fresh
Consumptions Varieties Suitable For Intercropping Resistance To
Disease ,Anthracnose,Cercospora Leaf Spot, FusariumWilt,Ascochyta
Blight Resistance To Insects Hairy Caterpillars, Leaf
Hoppers,Aphids,Thrips, Bruchids, Pod Borer Inheritance pattern
Growth habit Monogenic Flower colour Calyx colour
Pod number Non additive Pod length Partial dominance Pod colour No
of seed/pods Over dominance Pod yield/plant Additive gene action
Seed yield Biotic and Abiotic stress
Anthacnose, Rust, Cercospora leaf spot TVu -310, 315, Iron Fusarium
wilt TVu- 347,1000,Iron Bacterial blight TVu -347,410,Vita -3 Scab
TVu- 853,Vita- 4 Brown blotch Vita -1 BIOTECHNOLOGY Indirect marker
assisted selection can be useful for applying selection pressure in
early generations for trait that are difficult to select directly
or for shuttle breeding where it is not possible to directly screen
for resistances to a pest due to quarantine restrictions. Isozymes
have not been useful for indirect selection in cowpea due to
extremely low levels of polymorphisms in cultivated cowpea. DNA
markers should be more effective for developing a linkage map for
cow pea. A cow pea linkage map has been developed from a cross
between an improved cultivar anda wild sub species Vigna
unguiculata ssp.dekindtiana. This map consists of 87 random genomic
and 5 cdna RFLP 5 random amplified arranged in 10 linkage groups..
VARIETY S.N. Variety Method Characters 1. Pusa Phalguni
Selection From Dolique Du Tonkin Dwarf Bushy Habit,maturity in 60
days pods of cm length,yield potential of green pods is 75 q/ha 2
Pusa Barsati Exotic Selection From Phillipines Early Variety ,pods
are cm long ,yield potential 75q/hac 3. Pusa Do Fasli Pusa
Phalguni*Phillippines Plant Busy ,Photo Insensitive,pods are 18 cm
long pod yield 80 q/hac. 4 S-203 Selection-2*Virginia Double
Cropping System In Red Soils 5 Yard Long Bean Plants Need
Staking,pods are about 50cm long,pod yield 100q/hac. 6 Pusa Komal
Selection 1552 Resistance To Bacterial Blight,pods are light green
cm long ,pod yield 100 q/hac. 7 Pusa Rituraj Selection Plant Is
Bushy,Dual Purpose Variety 8 S-488 Virginia*Iron Grey Tolerance To
Xanthomonas Vignicola Lima bean S.N.-Phaseolus lunatus L.(sweet)
Family-Fabaceae 2n=2x=22
Central America(Mexico and Guatemala) for the small seeded forms
and the Andean region(Peru and Ecuador) for the large seeded. Self
pollination The world collection of germplasm is being maintained
at the Centro International de Agricultura
Tropical.(CIAT,Cali,Colombia). Small germplasm collections are in
other countries also like the USA,Brazil Costa
rica.Germany,Indonesia. Selection indices Plant Height Early
Maturity Short Duration
No of PodsPer Plant Pod Length No of Seeds Per Pod Resistance to
Insect Pest AndDisease Resistance to High Temperature Variety
Almost all varieties of lima bean in india are introductions from
abroad. Thesepole types like florida butter,challenger and Carolina
butter,semi pole types hopi and Wilbur and bush type like baby
potato, babyford hook and Henderson bush. Broad bean S.N.-Vicia
faba L. Family-Leguminosea Chromosome no-2n=12
Origin-Near east The broad been is not highly autogamous. It has
considerable natural cross pollination (20-40%) mainly by bumble
bees and honey bees. Variety:- Pusa sumeet Selection indices High
yield Desirable seed size and colour
High seed protein High methoinine and cysteine Low tannins
Earliness Stiff straw Resistance to charcoal spot, Ascochyta
blight, Rust,bean yellow mosaic virus Tolerance to drought and salt
Cluster bean Cymposis tetragonolobus(L.) Taub Family Fabaceae
Chromosome no 2n=12 Origin-Africa C.sengalensis is the ancestor of
cluster bean, but it is not found growing wild. Pollination:
Cluster bean is mostly self pollinating with only 2 percent cross
pollination Selection indices Plant type dwarf, single stem
Seasonal adaptability
Early maturity Pod quality-pod long smooth tender and fibreless
Green pod yield Resistance to insect pest variety S.N. Variety
Method 1. Pusa mausami Selection 2. Pusa navbahar
Pusa mausami*Pusa sadabahar 3. Pusa sadabahar Selection from a
local cultivar jaipuri 4 Durga bahar Pusanavbahar*ROC-401 Field
bean S.N.-Lablab purpureus L. sweet Family-Fabaceae
Chromosome no-2n=2x=22 Origin-India Pollination:-It is generally
selfpollinated. However, about 6-10% cross pollination is observed.
Flowers open generally two days after anther dehiscence opening is
mostly between 11 am to 5 pm. Inheritance pattern Spreading plant
habit Monogenic ,dominant
Growth habit and inflorescence type Three genes Photoperiod
sensitivity Monogenic, dominant Leaf vein colour Digenic
,complementary genes Purple petiole Two complementary genes Pod
form Monogenic,dominantand two complementary Pod colour Three
duplicategene Quantitative inheritance
Number of days to flowering pod length and width Additive
geneaction Number of pods per plant seed per plant and pod yield
High heritability, high genetic advances, additive gene action Seed
yield per plant High heritability and high GCV Pod yield per plant
Partial dominance with duplicate type of epistatis Seed yield
Complementary type of epistatis Number of flowers Non additive gene
action Selection indices Bushy plant habit Early maturity
Photoinsenstivity
Pod characters(shape, size ,colour) Number of pods per plant Seed
colour Number of seed per podgreen pod yielddry seed yield
Resistance todisease and pest (aphids and hairy caterpillar)
Variety Pusa early prolific Selection From Local Type Pusa
Sem-2
Co-1 Selection FromHD 18 Of Hisar Co-2 SelectionFrom Local Variety
Chhina Avarai Co-3 Selection From A Local Type Yanaikathu Avarai
Hebbal Avare-3 Hebbal Avare-1*Us-67-13 THANK YOU