BRIGHT FIELD MICROSCOPY

BRIGHT FIELD MICROSCOPY ANDRES, JAMAICAASUNCION, CRISTINE BOY, ELLEN KRISTINE

CORPUZ, DANICADAILEG, JUDIMELSANTOS, VIRGILIO

BRIGHT FIELD MICROSCOPYSIMPLEST OPTICAL MICROSCOPY ILLUMINATION TECHNIQUE

IT FORMS A DARK IMAGE AGAINST BRIGHT BACKGROUND

USES VISIBLE LIGHT AS SOURCE OF ILLUMINATION

COMMONLY USED TO VIEW LIVE AND IMMOBILE SPECIMENS SUCH AS BACTERIA, CELLS, AND TISSUES.

BRIGHT FIELD MICROSCOPYSTAINING OF ORGANISM IS REQUIRED

LIGHT SOURCE: EITHER A MIRROR OR AN ELECTRONIC ILLUMINATOR LOCATED IN THE BASE

IMAGE FORMED: RELATIVELY LARGE INTERNAL STRUCTURES AN OUTLINE CAN BE SEEN

TOTAL MAGNIFICATION: *IF 10X OCULAR MAGNIFICATION IS USED)RANGE: 10X 1000XHOW IMAGE IS FORMED:

DARK FIELD MICROSCOPYIT USES A DARK BACKGROUND WITH THE SPECIMEN BEING THE ONLY ONE ILLUMINATED.

USED IN OBSERVING UNSTAINED SPECIMENS

LIGHT SOURCE: LIGHT BULB FROM A MICROSCOPE

IMAGE FORMED: DARK BACKGROUND WITH ILLUMINATED SPECIMEN

TOTAL MAGNIFICATION: CAN RANGE FROM THOSE OF COMPOUND MICROSCOPE (10X TO 1000X)ONLY LIGHT THAT HAS BEEN REFLECTED OR REFRACTED BY THE SPECIMEN FORMS THE IMAGE

THE DARK FIELD MICROSCOPE CAN RAVEL CONSIDERABLE INTERNAL STRUCTURE IN LARGER EUKARYOTUC MICROORGANISMHOW IMAGE IS FORMED: