BRITISH SOCIETY FOR CELL BIOLOGY

SPRING MEETING

The next meeting of the Society will be held in Edinburgh on Thursdayand Friday, 30 and 31 March 1967.

There will be a one-day Symposium on The Cell Cycle, on 30 March,followed by a session of general papers on 31 March. Offers of papers forthe latter should be submitted not later than 15 January 1967 to:

D R E . J.AMBROSE

CHESTER BEATTY RESEARCH INSTITUTE

FULHAM ROAD, LONDON, S.W. 3

Tel.: FIAxman8133

Information about membership of the Society, which exists 'to promotethe advance of research in relation to all branches of cell biology and toencourage the interchange of information', may be obtained from theSecretary/Treasurer,

D R L . M. FRANKS

TISSUE AND ORGAN CULTURE UNIT

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THE JOURNAL OFEXPERIMENTAL BIOLOGYEdited by SIR VINCENT WIGGLESWORTH and J. A. RAMSAY

Vol. 45, No. 2 30s. net October 1966

HAMMOND, R. A. Changes of internal hydrostatic pressureand body shape In Acanthocephalus ranac.

HAMMOFTO, R. A. The proboscis mechanism of Aeantho-ctphaluj ranae.

KENNEDY, J. S. The balance between antagonistic induc-tion and depression of flight activity In Aphis fabaeScopoli.

WBEVERS, R . D E G . The physiology of a lepidopteranmuscle receptor. III. The stretch reflex.

BINOLBY, M. S. Membrane potentials in Amoeba proteus.

MACHIN, JOHN. The evaporation of water from Helixasperse. IV. Loss from the mantle of the inactive snail,

CHOMPTON, D. W. T. Measurements of gJucose and aminoacid concentrations, temperature and pH in the habitatof Polymorphic minutus (Acanthocephala) in the in-testine of domestic ducks.

MRLUER, P. L. The supply of oxygen to the active flightmuscles of some large beetles.

JOSEPHSON, ROBERT K. Neuromuscular transmission in a

sea anemone.GITTHRIE, D. M. Sound production and reception in a

cockroach.PILKINOTON, J. B. and SIMKISS, K. The mobilization of the

calcium carbonate deposits in the cndolymphatic sacsof metamorphosing frogs.

LA WRY, JAMES V. Jr. Neuromuscular mechanisms of bur-row irrigation in the Echiurold worm Urcchis caupoFisher and MacGinitie. I. Anatomy of the neuro-muscular system and activity of intact animals. II.Neuromuscular activity of dissected preparations.

TRUEMAN, E. R. The fluid dynamics of the bivalvemolluscs, Mya and Margaritifera.

Subscription for 1966y 80s. per volume of 3 parts, 2 volumes a year. Single parts 30s. net

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(vi)

Journal of CELLSCIENCEFormerly the Quarterly Journal of Microscopical Science

VOLUME 1, 1966

Editors:

H. G. CALLAN A. V. GRIMSTONE

Editorial Board:

G. H. BEALE

H. G. DAVIES

J. B. FINEAN

E. G. GRAY

H.HARRIS

H. E. HUXLEY

B.JOHN

J. M. MITCHISON

D. H. NORTHCOTE

J. PAUL

J. HESLOP-HARRISON SIR JOHN RANDALL

S. J. HOLT M. G. P. STOKER

Published for the Company of Biologists Limited

CAMBRIDGE UNIVERSITY PRESS

Printed in Great Britain at the University Printing House, Cambridge

C O N T E N T S

NUMBER 1 MARCH 1966

page i HARRIS, H., WATKINS, J. F., FORD, C. E. and SCHOEFL, G. I.Artificial heterokaryons of animals cells from different species

31 BEALE, G. H. and JURAND, A.

Three different types of mate-killer (mu) particle in Parameciumaurelia (syngen 1)

35 MITCHISON, J. M. and CUMMINS, J. E.

The uptake of valine and cytidine by sea-urchin embryos and itsrelation to the cell surface

49 SMITH, D. S., GUPTA, B. L. and SMITH, UNA

The organization and myofilament array of insect visceral muscles

59 SMITH, UNA and SMITH, D. S.

Observations on the secretory processes in the corpus cardiacum of thestick insect, Carausius morosus

67 CHARLTON, B. T. and GRAY, E. G.

Comparative electron microscopy of synapses in the vertebrate spinal cord

81 BENSON, KATHERINE A. and BENSON, D. G., JR.

A histochemical analysis of the proteins in the larval fat body ofSarcophaga butlata

85 CALLAN, H. G.

Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum

109 PICKETT-HEAPS, J. D. and NORTHCOTE, D. H.

Organization of microtubules and endoplasmic reticulum during mitosisand cytokinesis in wheat meristems

121 PICKETT-HEAPS, J. D. and NORTHCOTE, D. H.

Cell division in the formation of the stomatal complex of the youngleaves of wheat

129 FRIEDLANDER, M. and WAHRMAN, J.

Giant centrioles in neuropteran meiosis

NUMBER 2 JUNE 1966

145 MACPHERSON, I.

Mycoplasmas in tissue culture

169 HOUSE, W. and STOKER, M. G< P.

Structure of normal and polyoma virus-transformed hamster cell cultures

175 SINGH, S. B. and MUSGRAVE, A. J.

Some studies on the chromatin and cell wall of the mycetomalmicro-organisms of Sitophilus granarius (L.) (Coleoptera)

iv Contents

page 181 GRINYER, I. and MUSGRAVE, A. J.Ultrastructure and peripheral membranes of the mycetomal micro-organisms of Sitophilus granarius (L.) (Coleoptera)

187 MANTON, IRENE

Further observations on the fine structure of Chrysochromulina chiton,with special reference to the pyrenoid

193 PERRY, MARGARET M. and WADDINGTON, C. H.

The ultrastructure of the cement gland in Xenopus laevis

201 HOPKINS, C. R. and KING, P. E.

An electron-microscopical and histochemical study of the oocyteperiphery in Bombus terrestris during vitellogenesis

217 BARNICOT, N. A.

A note on the structure of spindle fibres

223 VAN HARREVELD, A. and MALHOTRA, S. K.

Demonstration of extracellular space by freeze-drying in the cerebellarmolecular layer

229 WESTRUM, L. E. and LUND, R. D.

Formalin perfusion for correlative light- and electron-microscopicalstudies of the nervous system

239 KRASNE, F. B. and LAWRENCE, P. A.

Structure of the photoreceptors in the compound eyespots ofBranchiomma vesiculosum

249 HAMORI, J. and HORRIDGE, G. A.

The lobster optic lamina. I. General organization

257 HAMORI, J. and HORRIDGE, G. A.

The lobster optic lamina. II. Types of synapse

NUMBER 3 SEPTEMBER 1966

271 HAMORI, J. and HORRIDGE, G. A.

The lobster optic lamina. III. Degeneration of retinula cell endings

275 HAMORI, J. and HORRIDGE, G. A.

The lobster optic lamina. IV. Glial cells

281 HADEK, R.

Cytoplasmic whorls in the golden hamster oocyte

283 CHANDLER, R. L. and WILLIS, R.

An intranuclear fibrillar lattice in neurons

287 FINEAN, J. B., COLEMAN, R., GREEN, W. G. and LIMBRICK, A. R.

Low-angle X-ray diffraction and electron-microscope studies of isolatedcell membranes

Contents v

page 297 STOKER, M. G. P., SHEARER, MOIRA and O'NEILL, C.Growth inhibition of polyoma-transformed cells by contact withstatic normal fibroblasts

311 ABBOTT, R. H. and CHAPLAIN, R. A.

Preparation and properties of the contractile element of insect fibrillarmuscle

331 DAVIES, H. G. and TOOZE, J.

Electron- and light-microscope observations on the spleen of the newtTriturus cristatus: the surface topography of the mitotic chromosomes

351 GRIMSTONE, A. V. and KLUG, A.

Observations on the substructure of flagellar fibres

363 GRAHAM, C. F.

The regulation of DNA synthesis and mitosis in multinucleate frog eggs

375 MANTON, IRENE

Observations on scale production in Prymnesium parvum

381 LEWIS, P. R. and SHUTE, C. C. D.

The distribution of cholinesterase in cholinergic neurons demonstratedwith the electron microscope

NUMBER 4 DECEMBER 1966391 SHAFFER, B. M.

Inhibition of aggregation of the slime mould Dictyosteliumdiscoideum by a factor diffusing from Escherichia coli

401 SCHNEEREBGER, E. E. and HARRIS, H.

An ultrastructural study of interspecific cell fusion induced byinactivated Sendai virus

407 VAUGHAN, R. B. and TRINKAUS, J. P.

Movements of epithelial cell sheets in vitro

415 MILLINGTON, P. F., CRITCHLEY, D. R. and TOVELL, P. W. A.

The role of calcium in the isolation of brush borders from epithelialcells of rat small intestine

425 GLAUERT, AUDREY M.

Moir^ patterns in electron micrographs of a bacterial membrane

429 MANTON, IRENE

Observations on scale production in Pyramimonas amylifera Conrad

439 GLIDDON, R.

Ciliary organelles and associated fibre systems in Euplotes eurystomus(Ciliata, Hypotrichida). I. Fine structure

449 EVANS, L. V.

Distribution of pyrenoids among some brown algae

vi Contents

page 455 BAJER, A. and ALLEN, R. D.Role of phragmoplast filaments in cell-plate formation

463 GUTHRIE, D. M.

The function and fine structure of the cephalic airflow receptor inSchistocerca gregaria

471 DICKSON, M. R.

A spiral sleeve in rotifer cilia tips

475 LAWRENCE, P. A.

Development and determination of hairs and bristles in the milkweedbug, Oncopeltus fasciatus (Lygaeidae, Hemiptera)

INFORMATION FOR CONTRIBUTORS

1 Manuscripts should be sent to The Editors,Journal of Cell Science, Department of Zoology,Cambridge, England.

2 Manuscripts must be typewritten, in doublespacing throughout (including tables, references andlegends). Each table should be typed on a separatesheet. Legends to figures should be typed in asingle series and placed at the end of the manuscript.Papers must be fully corrected by the author, and acharge will be made for excessive alteration in proof.

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6 The list of References must be given inalphabetical order of authors' names. The titles ofjournals should be abbreviated in accordance withthe World List of Scientific Periodicals, 4th ed. (1963).The following style is used:

BARNICOT, N. A. & HUXLEY, H. E. (1965).

Electron microscope observations on mitoticchromosomes. Q. Jl microsc. Set. 106, 197-214.

MAZIA, D. (1961). Mitosis and the physiology ofcell division. In The Cell, vol. 3 (ed. J. Brachet& A. E. Mirsky), pp. 77-412. New York andLondon: Academic Press.

Citations in the text are given in the following form:Jones & Smith (i960) or (Jones & Smith, i960).Where there are more than two authors the firstcitation should include all the names and subsequent

citations should be in the form (Jones et al., i960).Where more than one paper by the same author(s)have been published in the same year they are citedas Jones (1960a), Jones (19606) etc.

7 Text figures should preferably be drawn abouttwice final size; very large drawings should beavoided. Photographic reproductions of drawingscannot always be satisfactorily reproduced. Themaximum printed size of a drawing is 5 in. by8 in. Lettering will be inserted by the printers andshould be indicated on drawings in faint bluepencil or on a tracing-paper overlay. It should be inlower case, and abbreviations should not be used ifthere is space for complete words.

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