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The$delivery$of$conventional$nutrients$should'not'interfere'with'skin'physiology.$
Research$shows$that$regulating$the'com
position'of'intracellular'lipids'in$the$skin$can$increase$or$decrease$the$bioavailability$of$nutrients.$
Skin$delivery$could$also$be$addressed$with$ingredients$w
hich$decrease'the'diffusional'(electrostatic)'resistance'of'the'lipid'bi:layer$to$the$passing$m
olecule.$
IMPR
OV
ING
SKIN PER
MEA
TION
•Tetrahydropiperine$(THP) $1$is$a$stabilized'derivative'of'the'alkaloid'piperine$extracted$from
$the$fruits$of$black'pepper.$
•It$is$able$to$enhance'skin'natural'ability'to'absorb'nutrients.$
•All'the'ingredients$in$Beauty$Potions$range$provide'TH
P'inside.
Piperine
THP
1.$US$Patent$5744161$(1998),$M
ajeed$M.,$Badm
aev$V.$and$Rajendran$R.$(to$Sabinsa$Corporation)$
THP – SKIN
PERM
EATIO
N EN
HA
NC
ER
Reproduced$from$Brian$W
$Barry,$Breaching$the$skin's$barrier$to$drugs,$Nature'Biotechnology'2004,$22,$165$–$167.
Experiments$done$both$in$vitro$and$in$vivo$w
ith$the$parent$com
pound$piperine$indicate$that$Cosm
operine'may'operate'by'increasing'either'
of'two'events:$$
1.mem
brane'fluidity'2.
affinity'of'nutrient$to$the$cell$mem
brane1.
1Majeed$M
,$Badmaev$V,$Prakash$S,$Bioperine$N
ature’s$own$therm
onutrient$and$Natural$bioavailability$enhancer.$N
utriScience$Publishers,$Inc.$1999.$pp.$82.$
THP – SKIN
PERM
EATIO
N EN
HA
NC
ER
Effect$of$THP$on'penetration'of'green'tea'polyphenols'across$egg$mem
brane$in$Franz$Diffusion$System*$$
THE PER
MEA
TION
ENH
AN
CIN
G A
CTIV
ITY
*(V.$Badmaev,$M
.$Majeed,$«Skin$and$its$rle$in$providing$
nourishement$to$the$body$–$Pungent$principles$of$black$pepper,$
alkaloid$piperine,$and$tetrahydropiperine$(THP)$as$Enhancers$of$nutrients$and$drug$bioavailability$^$Cosm
etic$Science$Technology$2004)
•M
C2
^CLA
IM:''
The$skin$bio$revitalizer$^
ACTIV
ITY:$$Counterbalance$daily$energy$drops$by$waking$up$and$em
powering$
$$
cellular$metabolism
$
•X
TEND
^
CLAIM
:''The'skin$rescuer$
^ACTIV
ITY:''Skin$cells$and$cutaneous$architecture$protection$and$recovery'
FIRST LA
UN
CH
ES – IN C
OSM
ETICS 2015
Based$upon$the$combination$of:$
•Ginseng$saponins$$
•Açai$polyphenols$
already$known$in$literature$for$their$tonic 1$and$antioxidant$properties 2.$
APPLICATIO
NS:'
Skin$revitalizing,$«urban$proof»$products$
MC
2 FEATU
RES
1Tan$SJ,$Li$N,$Zhou$F,$Dong$Q
T,$Zhang$XD,$Chen$BC,$Yu$Z,$Ginsenoside$Rb1$improves$energy$m
etabolism$in$the$skeletal$m
uscle$of$an$animal$m
odel$of$postoperative$fatigue$syndrom
e,$J$Surg$Res.$2014$Oct;191(2):344^9.$
2$Bonomo$Lde$F,$Silva$DN
,$Boasquivis$PF,$Paiva$FA,$Guerra$JF,$Martins$TA,$de$Jesus$Torres$ÁG,$de$Paula$IT,$Caneschi$W
L,$Jacolot$P,$Grossin$N,$Tessier$FJ,$
Boulanger$E,$Silva$ME,$Pedrosa$M
L,$Oliveira$Rde$P,$Açaí$(Euterpe$oleracea$M
art.)$modulates$oxidative$stress$resistance$in$Caenorhabditis$elegans$by$direct$and$
indirect$mechanism
s,$PLoS$One.$2014$M
ar$3;9(3):e89933.
GIN
SENG
RO
OTS•
Country'of'Origin:$native$to$northern'hem
isphere'regions'of'A
sia'$(mostly$Korea,$N
orth$China$and$eastern$Siberia)$and$N
orth$America:$$typically$grow
s$in$colder$climates.$The$
species$Vietnamese$Panax$is$the$m
ost$commonly$used.$
•Tradition:$over$five$thousand$years$ago,$in$the$m
ountains$of$M
anchuria,$China,$Panax'ginseng$was$com
monly$used$
for$its$rejuvenating$powers.$The'traditional'Chinese'
medicine'considers'the'plant'an'elixir'of'youth,$w
hich$has$all$the$im
aginable$therapeutic,$preventive,$curative$and$energy$activities;$ginseng$appears$in$the$earliest$treatises$on$m
edicine,$at$the$beginning$of$the$Christian$era.$
•Main'properties:$tonic,$anti$fatigue
•Country'of'O
rigin:$Amazon,$Brasil$
•The'Plant:$palm
$tree,$produces$dark$purple$berries$
•Tradition:$Brazilian$natives$discovered$the$healing'properties$of$the$acai$berry$thousands$of$years$ago,$as$they$found$it$boosted$their$energy$w
hen$consumed$regularly.$$
•Main'properties:$thanks$to$its$com
bination'of'antioxidants,'am
ino*acids*and'omega'fatty*acids'all$help$slow
'the'aging'process'by$boosting'im
mune'and$m
etabolic'function'and$rem
oving'destructive'
free'radicals'
from'our'
bodies.$Nicknam
ed$this$superfruit$the$“Beauty$Berry”.$
AÇ
AI BER
RIES
In vitro
TESTS
•Cellular'm
etabolism'increase'24h
•Cellular'm
etabolism'increase'4h'
MC
2 TESTS
In V
ivo TEST
•Skin'Hydration''
•Skin'Extensibility'
•Skin'Gross'Elasticity'
•Skin'Brightness
Measurem
ent$of$ATP$is$considered$the$most$sensitive$
method$for$m
easuring$the$cellular$viability,$integrity$and$analyze$w
hether$a$substance$is$able$to$stimulate$the$
production$of$intermediates$of$cellular$energy$m
etabolism.$
Cells:$human$keratinocyte$cell$line$(HaCat).$
Timepoint:$24$h$
Measurem
ent:$bioluminescent$signal$derived$from
$the$transform
ation$of$luciferin$in$oxyluciferin$by$the$enzyme$
luciferase$in$the$presence$of$Mg2+$ions.$
Ce
llula
r me
tab
olism
inc
rea
se 24h
In V
itro
Measurem
ent$of$ATP$is$considered$the$most$sensitive$
method$for$m
easuring$the$cellular$viability,$integrity$and$analyze$w
hether$a$substance$is$able$to$stimulate$the$
production$of$intermediates$of$cellular$energy$m
etabolism.$
Cells:$human$keratinocyte$cell$line$(HaCat).$
Timepoint:$4$h$
Measurem
ent:$bioluminescent$signal$derived$from
$the$transform
ation$of$luciferin$in$oxyluciferin$by$the$enzyme$
luciferase$in$the$presence$of$Mg2+$ions.$
Ce
llula
r me
tab
olism
inc
rea
se 4h
In V
itro
20$wom
en,$between$35$^$65$years$old
T0,$T2weeks,$T4w
eeks
Twice$a$day$for$4$w
eeks
Half$Face$(each$subject$is$his$own$control)
5%$M
C2$cream
$Vs$placebo
Skin$elasticity,$hydration,$brightness,$self$assessment
ISPE$Institute$of$Skin$and$Product$Evaluation,$Milan,$Italy
MC
2 EFFICA
CY PR
OTO
CO
LIn
Vivo
MC2'is$able$to$perform
$a$statistically'significant'increase'in'skin'hydration'after'2'and'4'w
eeks'of'treatment.$M
C2'activity$leads$up'to'a'+'28%
'im
provement$in$hydration$in$com
parison$to$placebo.
Skin H
ydra
tion
In V
ivo
A'statistically'significant'decrease$in$skin$extensibility$(R0$param
eter)$was$evidenced$after$2$and$4$w
eeks$of$treatment.$$
Skin Exte
nsib
ility (R0) H
ydra
tion
In V
ivo
A'statistically'significant'increase'in$skin$elasticity$(R2$$param
eter)$was$
evidenced$after$2$and$4$weeks$of$treatm
ent.$At'4'w
eeks,$the'im
provement'w
as'almost'doubled.
Skin G
ross Ela
sticity (R
2)In
Vivo
After'4'w
eeks'of$treatment,$the$lum
inance$parameter$of$5%
'MC2'cream
$is$3'tim
es'higher'than'placebo.
Skin R
ad
ian
ce
4 we
eks
In V
ivo
After'4'w
eeks'of$treatment,$80%
'of'the'volunteers'have'declared'to'be'willing'to'use'again'the'product.
Co
nsu
me
r pe
rce
ptio
nIn
Vivo
TECH
NIC
AL D
ATA
MC
2
GLYCER
IN,'A
LCOHOL'D
ENAT.,'
TETRAHYD
ROPIPER
INE,'PA
NAX'G
INSEN
G'RO
OT'
EXTRACT,'A
QUA,'EU
TERPE'O
LERACEA
'FRUIT'
EXTRACT
Liquid$
Light$yellow$to$am
ber
2$^$5%
INC
I Nam
e
Aspect:
Colour:
Recom
mended use
PRIM
AR
Y SKIN
IRR
ITATION
TEST (in vivo): (patch test 0.5%
sol.)N
OT IR
RITA
NT
PRIM
AR
Y SKIN
IRR
ITATION
TEST: (hum
an reconstituted epidermidis)
NO
T IRR
ITAN
T
SKIN
SENSITISATIO
N (in vitro):
NO
T SENSITIN
G
MC
2 TOX
ICO
LOG
ICA
L PRO
FILE
Cla
ims
Skin'elasticity''enhancem
ent'
MC
2
Skin'firmness'
defender'Skin'R
adiance'im
provement
Skin'Eveness'source
Skin En
erg
y Boo
stCellular'm
etabolism'
awakening
Based$upon$the$combination$of:$
•Kuzu'isoflavonoids'(e.g.$puerarin)$$
•Cat’s'claw
'oxindole'alkaloids'and'quinic'acids$
already$known$in$literature$for$their$anti:glycation'potential'and$collagen'
renewal' 1$,anti:inflam
matory'and'protective'properties' 2. '
APPLICATIO
NS:'
Recovery,$soothing$and$protective$products,$night/weekend$form
ulations.$
1Kim$JM
,$Lee$YM,$Lee$GY,$Jang$DS,$Bae$KH,$Kim
$JS,$Constituents$of$the$roots$of$Pueraria$lobata$inhibit$form
ation$of$advanced$glycation$end$products$(AGEs),$Arch$Pharm$Res.$2006$O
ct;29(10):821^5.$2$Zhang$L,$Zhang$J,$Zhao$B,$W
ilson$XZ,$Quinic$Acid$Could$Be$a$Potential$Rejuvenating$N
atural$Com
pound$by$Improving$Survival$of$Caenorhabditis$elegans$under$Deleterious$Conditions,$
Rejuvenation$Res.$Dec$2012;$15(6):$573–583.
XTEN
D FEA
TUR
ES
•Country'of'O
rigin:$Kudzu$root$is$a$fast'growing'evergreen'vine'
originated'from'China'and$brought$to$the$U
nited$States$from$
Japan$in$the$late$1800s.$$
•Plant:$Pueraria'lobata''
•Tradition:$Kudzu’s$root,$flow
er,$and$leaf$have$been$used$in$Chinese$m
edicine$since$at$least$200$BC.$Actually$its$high'medicinal'
and'nutritional'value$have$earned$kudzu$herb$a$reputation$as$the$“Asian$ginseng”$and$“longevity$pow
der”$in$Japan,$where$it$is$
honored$as$the$“Royal$Special$food.”$$
•Main'benefits:$it$is$enjoying$great$popularity$in$the$w
est$and$Japan$for$a$variety$of$purposes,$e.g.$w
eight$loss,$breast$enlargem
ent,$hair$loss$treatment,$alcoholism
$prevention,$liver$tonic,$etc.$$
KUD
ZU R
OO
TS
•Country'of'O
rigin:$woody'vine'that$grow
s$in$the$tropical$jungles$of$South'and'Central'A
merica.'
•Plant:'Uncaria'tom
entosa$(popularly$known$in$English$as$cat's'claw
;$in$Spanish$as$uña'de'gato$)$w
hich$derives$its$name$from
$its$claw^shaped$
thorns.$
•Tradition:$traditionally$used$in$Peruvian$m
edicine$for$the$treatment'of'
a'wide'range'of'health'problem
s,$particularly$digestive$complaints$
and$arthritis$and$to$treat$wounds,$stom
ach$problems,$cancer,$and$
more.$It$has$only$recently$caught$the$attention$of$w
estern$herbalists$and$researchers.$Today,$m
ainly$by$word$of$m
outh,$it$has$become$one$
of$the$best$selling$herbs$in$the$USA.$
•Main'properties:$Antibacterial,$anti^inflam
matory,$antim
utagenic,$antioxidant,$antitum
orous,$antiviral,$cytostatic,$depurative,$diuretic,$hypotensive,$im
munostim
ulant,$vermifuge.
CA
T’S CLA
W R
OO
TS
In V
ivo TEST
Skin'Hydration*Skin'Density*Skin'Roughness
XTEN
D TESTS
In vitro
TEST
Citoprotective'activity*
In V
itro
Oxidative$stress$is$one$of$the$m
ain$causes$of$cellular$aging,$and$skin$ageing$from
$chronic$sun$exposure$is$a$clear$demonstration$of$this$
and$it$is$responsible$over$the$years,$of$wrinkles,$dryness$and$skin$
atrophy$(Gordon$J.R.$et$al.,$2012).$$
Cells:$human$keratinocyte$cell$line$(HaCat).$
Procedure:$incubation$with$the$ingredient$(24h)$and$
subsequent$exposure$to$UVA$(1J).$
Timepoint:$24$h.$
Measurem
ent:$transformation$of$the$dihydro^2'^7’^
dichlorofluorescein$diacetate$(H2DCF^DA)$into$a$fluorescent$molecule$(DCF)$in$the$presence$of$RO
S.$
Cito
pro
tec
tive a
ctivity
20$wom
en,$between$35$^$65$years$old
T0,$T2weeks,$T4w
eeks
Twice$a$day$for$4$w
eeks
Half$Face$(each$subject$is$his$own$control)
5%$XTEN
D$serum$Vs$placebo
Skin$hydration,$density,$roughness,$self$assessment
Skin$roughness,$skin$density
ISPE$Institute$of$Skin$and$Product$Evaluation,$Milan,$Italy
XTEN
D EFFIC
AC
Y PRO
TOC
OL
In V
ivo
XTEND'is$able$to$perform
$a$statistically'significant'increase'in'skin'hydration'after'2'and'4'w
eeks'of'treatment.$XTEN
D'activity$leads$up'to'a'+'25%
'improvem
ent$in$com
parison$to$placebo.
Skin H
ydra
tion
In V
ivo
A'statistically'significant'increase'in$skin$density$w
as$evidenced$after$2$and$4$weeks$of$treatm
ent$in$comparison$to$placebo.$A
t'4'weeks,$the'im
provement'w
as'doubled.
Skin D
en
sityIn
Vivo
A'statistically'significant'decrease$in$skin$roughness$(Sa$param
eter)$was$
evidenced$after$4$weeks$of$treatm
ent$in$comparison$to$placebo.$$
Skin R
ou
gh
ne
ssIn
Vivo
In V
ivo
After$4$weeks,$XTEN
D$is$able$to$visibly'im
prove'wrinkles'
appearance.$$Green/blue$areas$representing$skin$depressions$are$considerably$dim
inished$at$the$end$of$the$study.
T04'w
eeks
Primo
s Pico
Op
tica
l 3D Skin
M
ea
surin
g D
evic
e G
MS
Skin$treated$with$5%
$XTEND$show
ed$an$improvem
ent$of$the$sub$epidermal$low
^echogenic$band$related$to$skin're:densification.$$
''''''''Skin Ec
ho
ge
nic
ity
Digital$images$of$skin$
echogenicity$obtained$$by$U
ltrasound$$Scanner$$Derm
ascan$C$â$Ver.$3$(Cortex$Technology),$referred$to$the$area$treated$with$GEL$at$5%
$XTEN
D.
In V
ivo
After'4'w
eeks'of$treatment,$63%
'of'the'volunteers'have'declared'to'be'willing'to'use'again'the'product.
Co
nsu
me
r pe
rce
ptio
nIn
Vivo
INC
I Nam
e
Aspect:
Colour:
Recom
mended use
TECH
NIC
AL D
ATA
XTEN
D
GLYCER
IN,'PU
ERARIA'LO
BATA'RO
OT'EXTR
ACT,'
ALCO
HOL'D
ENAT.,'TETR
AHYD
ROPIPER
INE,'
UNCA
RIA'TO
MEN
TOSA
'EXTRACT
Liquid
Light$yellow$to$am
ber$
2$^$5%
PRIM
AR
Y SKIN
IRR
ITATION
TEST (in vivo): (patch test 0.5%
sol.)N
OT IR
RITA
NT
PRIM
AR
Y SKIN
IRR
ITATION
TEST: (hum
an reconstituted epidermidis)
NO
T IRR
ITAN
T
SKIN
SENSITISATIO
N (in vitro):
NO
T SENSITIZIN
G
TOX
ICO
LOG
ICA
L PRO
FILE
Smoothness$supporter
Skin$defender
Extracellular$matrix$fixer
Skin$firmness$booster
Youth$preserver
Skin$relief$promoter
XTEN
D C
laim
s