PRODUCT BULLETIN Human Identification

Facilitating high-throughput

automation

It is becoming ever more important

for human identification laboratories

to efficiently provide high-quality

results from an increasing volume

of samples. This is primarily due

to the creation and expansion of

forensic DNA databases in many

countries worldwide. With further

expansion expected over the next

five years in the United States and

other countries, many laboratories

have implemented automation into

their workflows to help process the

growing number of samples that

require analysis.

Automating the front end of the single-source sample workflow has been shown to be critical to streamlining the entire process, while also maximizing sampling integrity and reliability. Automated sample punch instruments made by BSD Robotics are the industry-leading solution for automated sampling of dried biological specimens, and Life Technologies is now the exclusive distributor and service provider for the BSD600 and BSD1000 Punching Systems for forensic and human identification applica-tions in many countries, including the United States, Canada, and Mexico.

BSD Punching Systems Facilitating automation. Maximizing sample integrity.

Multiple configurations and features to suit your laboratory’s needsBSD Punching Systems have been designed for fast and reliable punching of dried biological samples, and are available in a semi-automated platform for medium-throughput laboratories (BSD600-Duet

Series II), and a fully automated platform for higher throughput laboratories (BSD1000-GenePunch). In addition, they are equipped with a variety of features that optimize high-throughput sampling perfor-mance, integrity, and efficiency.

BSD600 Duet

Key features and functions

Feature Description BSD600-Duet Series II BSD1000-GenePunch

Semi-automated platform Designed for medium-throughput laboratories X

Fully automated platformLoad and walk-away functionality; designed for higher throughput laboratories

X

Light targeting systemIlluminates the exact sizes and positions on the sample card from which the disks will be punched

X

Scanner moduleAutomatically scans sample areas on the card and determines the positions to punch full sample disks

X

Auto-trigger systemActivates the punching process automatically when a sample card is detected

X X

Anti-static SystemHumidifier system to help minimize the effects of static electricity on punched disks

X X

Disk detector system Detects that punched disks are deposited into the wells X X

Punch cleaning systemSpecially designed to minimize cross-contamination between samples

X X

Plate capacityHolds a range of 96-well and 384-well plates, including PCR plates, microplates, and deep-well plates

2 6

Barcode scannerEnables positive identification of plates, samples, controls, and standards

X X

Simple operation & maintenanceBasic operational training provided during installation; warranty and maintenance contracts available

X X

A B

Figure 1. BSD600 light targeting system (A) and anti-static system (B).

Figure 2. BSD1000 card magazine.

Key features and functions

Feature Description BSD600-Duet Series II BSD1000-GenePunch

Semi-automated platform Designed for medium-throughput laboratories X

Fully automated platformLoad and walk-away functionality; designed for higher throughput laboratories

X

Light targeting systemIlluminates the exact sizes and positions on the sample card from which the disks will be punched

X

Scanner moduleAutomatically scans sample areas on the card and determines the positions to punch full sample disks

X

Auto-trigger systemActivates the punching process automatically when a sample card is detected

X X

Anti-static SystemHumidifier system to help minimize the effects of static electricity on punched disks

X X

Disk detector system Detects that punched disks are deposited into the wells X X

Punch cleaning systemSpecially designed to minimize cross-contamination between samples

X X

Plate capacityHolds a range of 96-well and 384-well plates, including PCR plates, microplates, and deep-well plates

2 6

Barcode scannerEnables positive identification of plates, samples, controls, and standards

X X

Simple operation & maintenanceBasic operational training provided during installation; warranty and maintenance contracts available

X X

A complete system solution for forensic DNA databasing By leveraging state of-the-art PCR amplifica-tion technology, the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit is designed to amplify the 16 loci included in the Identifiler® kit from single-source samples spotted on FTA® Cards, without the need to perform any DNA extraction or purification. Blood or buccal samples on FTA® Cards can be punched directly into PCR plates or tubes for PCR amplification, with no reduction in data quality (Figure 3).

When BSD Punching Systems are paired with the AmpFℓSTR® Identifiler® Direct Kit, this complete system solution can help laboratories worldwide meet the increasing challenges of developing or expanding a forensic DNA database.

Industry-leading automated punching solutionsBSD Punching Systems are installed in more than 50 forensic sites worldwide (Figure 4). These high-quality punching instruments have been designed for fast and reliable automated sampling of biological specimens used for DNA databasing and other single-source sample applications, facilitating automation while maximizing sample integrity.

Figure 3. The complete AmpFℓSTR® Identifiler® Direct workflow. A punch from a blood or buccal sample spotted onto an FTA® Card can be used directly for PCR amplification, without any reduction in data quality. This dramatically simplifies the workflow for the processing of single-source samples, enabling users to simply “punch and go”.

Figure 4. Countries around the world in which a BSD Punching System has been installed (shaded).

THE COMPLETE IDENTIFILER® DIRECT WORKFLOW

Punch 1.2 mm Disc From Blood or Buccal Sample on FTA® Card Using BSD Punching System

Add Identi�ler® Direct Reagents

Amplify

Electrophorese

International Sales For our office locations please call the division headquarters or refer to our website at http://www.lifetechnologies.com/about-life-technologies/contact-us.html

Headquarters5791 Van Allen Way | Carlsbad, CA 92008 USA Phone 760.123.4567 | Toll Free 800.123.4567 www.lifetechnologies.com

During the development of products for forensic DNA analysis, Life Technologies performs developmental validation studies according to the validation guidelines provided by the Scientific Working Group on DNA Analysis Methods (SWGDAM). However, customer agrees it is the responsibility of customer to perform its own analysis and appropriate internal validation studies to ensure that the products and services it obtains from Life Technologies satisfy or will satisfy the SWGDAM Guidelines or other applicable guidelines used by the forensic community and are fit for its human identification applications.

For Research, Forensic, or Paternity Use Only. Not for use in animal or human therapeutic or diagnostic use.

NOTICE TO PURCHASER: Limited Use Label License

The products shown in this brochure may be covered by one or more Limited Use Label License(s). Please refer to the respective product documentation or the Applied Biosystems website under www.appliedbiosystems.com for the comprehensive license information. By use of these products, the purchaser accepts the terms and conditions of all applicable Limited Use Label Licenses. These products are sold for research use only, and are not intended for human or animal diagnostic or therapeutic uses unless otherwise specifically indicated in the applicable product documentation or the respective Limited Use Label License(s).

Not for re-sale.

The trademarks mentioned herein are the property of Life Technologies Corporation unless otherwise noted.

© 2010 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.

CO15361 1010

Technical specifications for BSD600-Duet Series II*

• Tray capacity: Two sample bays, capable of accommodating 2 standard microtiter (96-well) trays, deep-well plates, PCR plates, or 1 custom-designed tube holder accommodating 80 x 2 mL tubes

• Dual-punch system combinations: 1.2 mm/1.2 mm, 1.2 mm/1.5 mm. 1.2 mm/2.0 mm, 1.2 mm/3.2 mm, 3.2 mm/4.7 mm, or 3.2 mm/6.0 mm

—1.2 mm punch has been validated for use with the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit

• Power requirements: 110–240 volts (general purpose electrical outlet)

• Punch activation: Auto-trigger system and foot/hand operation• Computer software: Supplied by Life Technologies:

—BSD600 software, supplied on CD, based on Microsoft Windows 98 or later —Flexible, user-friendly guided user interface —Flexible plate layouts/templates—allows punching according to input files —Automatically creates output files; can be integrated with LIMS and/or downstream instruments

• Computer hardware (supplied by customer): —PC-compatible —Minimum system requirements:

–– Windows–98–or–later–– Pentium–800–MHz–with–SVGA–capability–– Color–monitor–(minimum–resolution–800–x–600–dpi)–– CD–ROM–(minimum–12X–speed)–– 3.5”–FDD–– 1–GB–HD–(15–MB–free–space)–– 32–MB–RAM–– 1x–9–pin–serial–port–(RS–232–Interface)–– 1x–keyboard

• Barcoding: System is supplied with one barcode scanner: —Can be turned on and off —Operates at 5 volts

• Disk detector: System is supplied with a disk detector comprised of a number of sensors (located in the lower section of the chute) designed to detect that a disk has been punched, and automatically re-punch up to three times if a disk is not detected.

• Patented air system: Incorporates a low-pressure air pump. The system assists in stripping the punched disks from the cutting edge of the punch. Includes humidifier system to minimize effects of static electricity.

• Physical dimensions: 400 mm (l) x 500 mm (w) x 400 mm (h), 35 kg• Installation, commissioning, and training (installation is included in

price): —To be undertaken by Life Technologies Field Service Engineer —Up to 4 hours operator training provided at time of installation included in price

• Warranty: A 12-month warranty is included. This covers parts, labor, and up to three preventative maintenance visits.

* Technical specifications for BSD1000-GenePunch are available upon request. Please contact your local sales representative for more information about the BSD1000-GenePunch or for ordering information on any of the BSD punching systems described in this brochure.