Spectrophotometer

By yonis NuradinProf. Heather Kundzicz

Prof. Sarah Feinman Bridges 13, 2014

Kenneth Campbell, PhD Professor of Biology -- Reproductive Endocrinology Areas of Expertise

Reproductive Endocrinology Degrees

PhD, Biochemistry, University of Michigan Ann Arbor MS, Biochemistry, University of Michigan Ann Arbor Work Experience

2005-2008 Associate Dean of Science and Mathematics, University of Massachusetts, Boston

MENTOR

Spectrophotometry spectrophotometer is the quantitative measurement of

the reflection or transmission properties of a material as a function of wavelength.

Turned spectrophotometer on and allowed to warm up for at least 10 minutes.

Set the wavelength using the keypad. Open compartment , and place a cuvette containing your blank.

Close the sample compartment door and press the( 0 ABS/100% T) button to blank the spectrometer at your chosen wavelength. The display will read 0.000 A (or 100.0 T)

How to operate a spectrophotometer?

Now is ready to make an absorbance measurement on the sample.

Fill the cuvette with the sample and Continue to make measurements at this wavelength without re-blanking the instrument

If need to change the wavelength then to re-blank the spectrophotometer at the new wavelength.

To determine the absorbance of unknown make standard curve graph from data recorded then, determine the concentration of Unknowns either by estimation or by linear regression.

Spectrophotometer