Các phương pháp lai phân tử

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  • 7/24/2019 Cc phng php lai phn t

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    Chng 6 PHNG PHP LAI PHN T

    1.KHI NiM LAI PHN T-Khi 2 mch DNA tch ri, nhit c lm gim t t, cng vii !in th" nghim th"ch h#, h$i mch %& '(t c)# tr* l$i+

    -)c im.)c hi t/t 0i. 1 ti '(t c)# ch3 4/ r$ gi5$ 2 tr6nh t h7n

    t7n '8 %ng vi nh$+

    9c tr6nh t '8 %ng c: th l DNA h7)c ;NAh6nh thnh cc#h

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    Nhit

    B0c #hn Cng l$i #hG thc nhit + BhHng thng t0c

    #hn Cng l$i cc i * nhit th?# hIn Bm !h7ng 2J +

    i cc tr6nh t

    B0c l$i tng t3 l thLn vi cn '6nh #hIng cM$ i cc

    tr6nh t '8 %ng+

    c i7n

    NEng N$9l OP lm tng t0c #hn Cng tng lQn t J Rn OS

    lFn+ NEng N$9l vt T O,2 P h7n t7n !hHng c: tc Gng+

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    U+ KV thLt 17thWrn Xl7t

    YhIng #h# n/ c %= Gng @nh v@ nh5ng tr6nh t )c 'it

    trQn DNA ' gWnW, h$/ nh5ng DNA !"ch thc nhZ hIn DNA

    #l$%mi, #h$gW[

    9I %* . !V thLt ch/n DNA t gWl lQn mng l$i+

    F tiQn, DNA c #h

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    9ch tiRn hnh.

    - DNA ' gWnW c c(t thnh nh5ng 7n c: !"ch thc !hc

    nh$ '*i Wn^/mW c(t gii hnin i #h

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    K, "h!-" /!"h0n '/"

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    + KV thLt N7rthWrn 'l7t+

    YhIng #h# n/ c %= Gng 4c @nh !"ch thc v hm

    lngcM$ mt m;NA )c trng tr7ng mt hdn h# ;NA+9ch tiRn hnh.

    - in i ;NA trQn gWl c: ch?t lm 'iRn t"nh#h ve cc liQn !Rt

    /R Ti @nh c? trfc 'Lc 2 cM$ ;NA+

    - 9h/n ;NA lQn m$ng l$i

    - BiRn hnh l$i vi m_ ` c: nh ?+

    - Yht hin t"n hi l$i

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    KV thLt N7rthWrn 'l7t+

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    J+ KV thLt W%tWrn 'l7t+

    YhIng #h# #ht hin #r7tWin trQn gWl #7l/$cr/l$miW ch/n Rn mt mHi trng 'n v5ng hIn v c0 @nh\v" G. mng nitr7cWlll7%W] vi nh5ng ng/Qn t(c

    tIng t nh 17thWrn 'l7t+

    9ch tiRn hnh.

    Bh nhLn #r7tWin t tR '7 nHi c?/4= l #r7tWin \%= Gng 1D1, nhit , DBB 'iRn t"nh #r7tWin]+

    BiRn hnh in i 1D1-YAj#h

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    KV thLt W%tWrn 'l7tBh nhLn v in i #r7tWin

    $i v #ht hin

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    k+ 9c h th0ng nh ? th0ng nh ? !hHng ng Eng v@ #h:ng 4.

    - 7r%Wr$i%h #Wr74/$%W \;Y] v chWmilminW%cWncW

    HRP oxi ha c cht (L!ino"#c cht $ t%&ng th'i )ch th)ch* ph't +'ng $ ,i +ng -./ n!0

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    th0ng 'i7tin %trW#t$viin1trW#t$viin l mt #r7tWin c: trang lng #h

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    th0ng nh ? #h:ng 4

    Br7ng #hIng #h# n/ ngi t$ %= Gng mt l7i #him #ht hin

    om_ `p\#r7'W] c nh ? 'qng YU2, #him n/ r?t nh/ cmvi 'Cc 4+