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CCB-TOX
Continuous Contamination Biomonitor
Benefits, Functions & Applications
Confidential & Preliminary
Contents
• Introduction
•Function
•Application
•Advantages
CCB-TOX
Introduction: what is the CCB-TOX?
• Automatic water toxicity biomonitor
• Continuously monitors chemical
contamination events.
• On-Line - sends alerts in real-time.
• Key element of an Early Warning
System. CCB-TOX
Introduction: key benefits
Significantly reduce the threats associated with accidental and
intentional chemical contamination of water:
• Spills, accidents, dumping
• Equipment malfunction
• Natural disasters
• Sabotage & terror
• Acute & chronic exposure
• Illness & death
• Direct & indirect costs
• Liability suits
Introduction: key uses
• Drinking water monitoring: protecting public health by sensing changes
in water quality at reservoirs, intake, during & after treatment, throughout
the distribution network, protected zones, etc.
• Environmental monitoring: protecting the environment by sensing
changes in water quality along rivers, lakes & natural reservoirs, as well
as near potential effluent discharge areas.
• Water treatment monitoring: improving treatment processes
effectiveness by sensing water quality changes at intake, and during
treatment processes, enabling process modifications decisions & quality
assurance.
Introduction: potential users
• Water companies: raw water suppliers, drinking water utilities,
municipal water suppliers, water treatment plants.
• Authorities: environmental supervising & monitoring, river basin
monitoring, health supervising, municipal water systems.
• Secured facilities: industrial zones & parks, hospitals, governmental,
military.
• Industrial companies: water intake & discharge for bottlers, food and
other water related processing companies.
Introduction: Key Features (1)
On line, continuous monitoring, fully automated.
Highly sensitive, detects real toxicity.
Detection of a very broad range of contaminants.
Very fast detection & warning: less than 15 minutes.
Minimal false positive & negative response.
High overall system reliability.
Suspicious samples are captured by an automatic sampler for
further analysis.
Introduction: Key Features (2)
Remotely operated & controlled.
Requires minimal operational intervention.
Very easy to use - no special skills required.
Easy installation & maintenance.
No need for adjustments.
Easily integrated with other systems.
CCB-TOX is an automatic toxicity biomonitor based on
Bioluminescence.
Toxicity is the degree to which a substance is able to damage an
exposed organism.
A biomonitor (biological monitor) uses an organism (such as fish,
daphnia, ,mussels, algae, bacteria) that provides information about the
quality of the environment (i.e. water) around it.
Bioluminescence is a naturally occurring process by which a living
organism converts chemical energy into light.
Function – basics (1)
CCB-TOX is based on luminescent (light
emitting) natural bacteria.
Changes in the level of bacterial
luminescence (light) indicate potential
toxicity in the sampled water.
CCB-TOX is able to measure and analyze
changes in light levels, and as a result
transmit an alert in case of toxicity.
Function – basics (2)
Decreased luminescence
Luminescence Level reflects degree of toxicity
Function – luminous bacteria (1)
Pesticides, HerbicidesChlorinated hydro-CarbonsGasoline, petrol oilHeavy metalsDetergents
Clean sample
Contaminated sample
Membrane function, Protein & lipid synthesis ATP generation, Electron transport, Cell respiration
affecting
Freeze dried luminous bacteria are stable for long periods.
A million cells are introduced into a small water sample, resulting in
reduced effect of biological variability.
Chemical toxicants that effect cell’s metabolism lead to rapid decay of
luminescence.
High correlation exists between the luminescence test and bioassays that
apply higher organisms, such as fish.
Function – luminous bacteria (2)
“Bacteria-based biosensors are ideal for use as early warning screening tools for drinking
water security” (USEPA).
Function - Luminous bacteria (3)
Strain namePhotobacterium leiognathi
Vibrio fischeri
OriginSea water
Optimal temperature for light emission
18-35°C15-16°C
Toxicity test utilized in
PCB-TOX (ToxScreen3)
PCB-TOX-SPOT
CCB-TOX
MicrotoxTM
ToxControlTM
LumisToxTM
BioFixTM
Stability to changing environmental conditions
HighLow
Function - proprietary biotechnology
Reaction to a wide spectrum of chemical agents.
Sensitive to very low concentrations of toxicants.
Results are obtained in minutes.
Reliable and consistent results.
Preliminary discrimination between cationic heavy metals & organic
toxicants.
Long term stable storage of biosensor bacteria
Basic technology (ToxScreen) verified by the USEPA-ETV.
Examples of Detected Toxic Chemicals
Toxic
chemical1
Rat LD502
(mg/kg body weight)
CCB-TOX
IC50 (mg/L)
Potassium cyanide100.5
Arsenate(5)140.09
Cadmium(2)2250.2
Dieldrin461.2
Parathion143
Methiocarb600.08
Chlorpyriphos820.7
Note-1 - chemicals that might be used for intentional contamination according to US Army reportsNote-2 - lethal dose data taken from MSDS provided by manufacturers
1)Connected to inlet water and clean reference water. Continuously
(365/7/24) draws samples (at predetermined intervals) into two assay
chambers to detect: cationic heavy metals and organic toxicants.
2)The light sensor (PMT) records emitted luminescence and compares it to
the level obtained in the reference reading.
3)To minimize false alarms, once a toxicity state is noted (e.g. 50%
luminescence change), a repeat reading is taken and then a repeat
reference test. If the result repeats itself, a toxicity alarm is turned on,
4)The suspected sample is drained into the grab sample container for future
confirmation and analysis.
Function – monitoring process
Function – monitoring specs.
• Bioassay: freeze-dried luminous bacteria.
• Buffer system: dual - cationic heavy metals & toxic organic chemicals.
• Detector: photomultiplier tube.
• Contaminants detected: > 150 toxic chemicals tested so far.
• Sensitivity: for most chemicals, detection ranges from ppb to low ppm.
• Measuring interval: programmable.
• Measuring time: 5-60 minutes.
Function – unique features
High reliability & low maintenance:
Automatic positive control tests.
Double check before alert signaling.
Automatic cleaning & flushing.
Internal electronics self test mechanisms.
Replacement of all biofouling-prone elements – every 4 weeks.
Automatic remote signaling (alarms) of instrument malfunction.
Minimal hands on required for operation & maintenance.
Quick and easy monthly cartridge replacement//
Function – key elements
Key elements include:
Static unit includes various hardware components (cabinet,
electronics, mechanics).
Cartridge unit includes re-fill reagents (bacteria & buffers)
and replaceable tubing.
Management software includes various options – either
embedded or server-based components.
Function – system overview
Function – outside view
Function – connections
RS232 to GSM
RJ45 to LAN
Inlet water
Outlet water
Grab sample water two
Reference water
Grab sample water one Wash water
E s c E n t e r
AquaVerityCCB Continuous Contamination Biomonitor
Contamination Alert
All Clear
SystemMalfunction
Function – front panel
• Bright alert lights.
• Complete local set up and
control using easy press
buttons.
• Basic LCD - 4 lines x 20
characters, support
European fonts.
• Optional LCD with support
of Chinese / English – 2
lines x 8 characters,
(128x32 dots)
Operation – control panel
Operation buttons:"Enter" – choose command /
enter selected screen /
approve parameter.
"Esc" – back to previous screen /
silent buzzer.
"▲►" – rolling lines up / move to next character position on the right.
"▼" – rolling lines down /
character and digit rolling
cycle.
"Enter" + "Esc" – LED's + buzzer test.
E s c E n t e r
AquaVerityCCB Continuous Contamination Biomonitor
Contamination Alert
All Clear
SystemMalfunction
Operation – LCD display screens
Function – static unit
• Cabinet with display
• Microprocessor
• Light detection module (PMT)
• Heating/cooling units
• Connections to inlet/outlet tubes
• Gantry, Motors
• Grab samples containers
Function – hardware specs.
• Power Supply: 90-264 VAC (~690VA) up to 250W. DC output – 12V.
• Hardware: specially designed control card (microprocessor,
integrated keyboard and graphic LCD display) and motor card.
• Data storage: 256K Flash, 32K internal RAM, 1024K external RAM,
(downloadable - automatically / remote PC poll).
• Alarms: warning light +buzzer, LCD display, external communication
(user programmable).
• UPS – such as Advice PRPC750 or similar (required).
• Safety compliance: CE (EU) & UL (USA).
Preliminary – specification subject to change without notice
Function – physical specs
• Enclosure: IP55; IP65 on request
• Total weight: ~ 75 Kg without reagents
• Shipping weight: ~ 100 Kg.
• Size: 800x800x500 mm (H*W*D)
• Mounting: wall or rack mount
• Operating temperature: 5°C - 40°C
• Operating humidity: up to 95%
Preliminary – specification subject to change without notice
Function – hydraulic specs.
• Flow Rate: at least 60 Liter/hr. Pressure : 0.5-2 bar.
• Sample delivery: Inlet water pressure: 1.5 – 2 bar, Temperature: 5°C-
50°C, Volume: –at least 180 Liter/hr.
• Water connections: Standard 8x6 mm (N*), permanent female thread ¼
BSP + nipple. Various diameters supported.
• Waste: pressure free 16X14 mm; total accumulated volume (including
inlet, wash, and reagents) - 150Liter/month.
• Reagents heater/cooler: thermo-electric cooler.
• Maintenance interval: once every 4 weeks, ~15 minutes.
• External containers: grab sample, wash, reference water. Preliminary – specification subject to change without notice
Function – communication specs.
• Remote control: SMS (Short Message Service) over GSM (using
a 3rd party modem, i.e. EZ10 by Telit.).
• Data communication protocols: TCP/IP, Ethernet, GSM, SCADA
• Physical Communication port: Ethernet (RJ-45), RS-232
• Data reporting interval: every 24hrs
• Modbus protocol for connection to SCADA systems (optional
supported with CAS).
Preliminary – specification subject to change without notice
Function - cartridge
• Assay Buffers containers
• Freeze dried bacteria vials
• Syringes
• Assay chambers
• Disinfection Solution container
• Reference water container
• Bacterial suspension chamber
Function – Test cycle preparation
Toxicity/Reference test
• Dispense 200μl Metal/Organic buffer
• Dispense 800μl Reference or inlet water
• Mix by air bubble
• Dispense 15.5μl Hydrated bacteria solution
• Mix by air bubble
• Enter into the PMT box and read the luminescence every minute during 15 minutes
Positive control test
• Dispense 200μl Metal/Organic buffer
• Dispense 100μl Disinfection buffer (1:9 dilution)
• Dispense 700μl Reference water
• Mix by air bubble
• Dispense 15.5μl Hydrated bacteria solution
• Mix by air bubble
• Enter into the PMT box and read the luminescence every minute during 15 minutes
Org
ani
c Disin
fection
Metal
Referen
ce
Inlet
Function – cartridge reagents
Each cartridge contains a 4 weeks supply of the following reagents
(shipped at ambient temperature):
• Freeze dried bacteria vials (4).
• Pro-Metal Buffer
• Pro-Organic Buffer
• Disinfection (cleaning)/Positive control Solution
• Hydration/Storage Buffer
Two key options for management
software of the CCB:
• Basic control software.
• Advanced Control & Analysis
software – CAS.
Function - management software
Preliminary – specification subject to change without notice
Basic control software:
• Basic presentation of test results in graphical form. Remote set up
and control.
• Device-based, fully integrated, internal web server.
• IP-based proprietary communication protocol.
• One to one device management, configuration and reporting.
• SMS alerts.
• Supported languages: English, French, German, Italian, Spanish &
Chinese. On-line help file.
Function – basic software
Operation – Web server pages
Entrance Page – Password and authorities
Function – basic software
Function - Alarm state & Alert SMS messages
Alarm messageAlarm typeCasesToxicity alert – MetalicToxicityOrganic water contamination.Toxicity alert – OrganicToxicityMetalic water contamination.
Instrument malfunctionMalfunctionPositive control not working.Motor failure or Microcontroller's failure.
Instrument reduced functionalityMalfunction
Abnormal change (ascend or descend) in light level in reference water (relative level).Initial reference water light level is too low after inserting a new cartridge.
Temperature off limitsMalfunction
TEC (Thermo-Electric Cooler) failure. Temperature in bacteria chamber, assay chamber, storage buffer, inlet water and F.D. bacteria is off limits for a predefined period of time after power return.
Unauthorized cartridge insertedInformativeUn-authorized (damaged or used) cartridge unit insertion.
Cartridge needs replacementInformativeReagents volume reaching low level.Cartridge hasn't been replaced on time.
Power failure InformativePower supply failure.
Power failure recoveryInformativePower supply returned after failure.
Water flow problemInformativeNo inlet water flow.No reference water flow.
Unauthorized door open InformativeUnauthorized door open.Interlock cut off.
Function – advanced software
Preliminary – specification subject to change without notice
CAS –
Control &
Analysis
Software
Key features:
Easy remote monitoring and control of multiple CCB units.
Intuitive data report generation and trend analysis.
Alert management: e-mail, text-messages, cellular.
Additional features: advanced backup; log file analysis; open interfaces, API.
Alert management.
Smooth integration and communication with 3rd party devices & management
systems (such as SCADA/GIS).
Fully customized. Secure and reliable.
Function – CAS
Preliminary – specification subject to change without notice
Management, Communication and Security:
Application is managed either on external server (ASP / central database) or on
customer’s server on-site (application and database with remote installation &
support).
Connectivity - Either LAN (gateway & firewall), Cellular communication
(GSM/GPRS/CDMA/1X/UMTS/EVDO) or Satellite communication.
Access via iCex controller (scheduled polling of CCB units) using either Modbus /
TCP / COMP. No firewall settings are required. COMP communicated with
customers’ central system (SCADA)>
Secured data access (authorization levels, SSL) and integrity (central backup /
restore & disaster / recovery mechanism).Preliminary – specification subject to change without notice
Function – CAS
Function – CAS
Customization:
Fully customized: CCB parameters, user interface.
Different polling schedule per CCB data element / value.
Flexible format & validity checks per data element.
Change history per data element.
Customized user interface.
Secured access from anywhere: PC, Cell phone, PDA.
Simple and straightforward:
Physical mounting. Electricity, UPS and internet connection.
Plumbing: inert filter, external containers (3), pressure regulator.
Cartridge mounting. Assay vials insertion. Gantry positioning. (every
4 weeks – about 15 minutes work).
Parameters set up: general. Testing and communications.
Total first installation time: < 2 hours.
CCB - Installation & maintenance
Accessories & add-ons
• Alternative / renewable power supply (i.e. solar).
• Communication option: wire and wireless.
• Inlet water self cleaning filters.
• Inlet water pressure reducer.
• Water / wash containers.
Preliminary – specification subject to change without notice
CCB - Future Options
Detection of microbial outbursts in drinking water.
Tailor-made tests for designated groups of toxic chemicals
of interest.
Detection of bacterial re-growth potential / bio-stability of
drinking and process water.
Preliminary – specification subject to change without notice
Application
Key part of a comprehensive Early Warning Solution:
• Effective coverage of drinking water systems.
• Located at various stations throughout the water distribution system,
• Coupled with Portable Contamination Biomonitors.
• Pinpoint contamination boundaries & trace contamination sources.
• Sseamless integration with other monitors / sensors and customer
management systems (i.e. SCADA).
AquaVerity
xxx
AquaVerity Components
CCB - Continuous Contamination Biomonitor
PCB - Portable Contamination Biomonitor
CAS - Control & Analysis Software package
SIS - Solution Implementation Service package
Tiered Response Model
Increasing:
Certainty
Response
Cost
Observed Water Quality Change
)determined by broad-based
continuous screening(
Automated Sample Collection
Confirmation Bioassay
Chemical Analysis
If positive
Public health Regulatory or Remedial Action
If positive
PCB-TOX-SPOT
CCB-TOC
Applications
CheckLight’s Portable Contamination Biomonitors:
PCB-TOX-SPOTPCB-TOX PCB-AOC
How does the AquaVerity solution
compare to other solutions?
EWS Matrix (1)- Detection & Warning Capabilities
FactorMulti-ParameterOther Biomonitors
CheckLight’sAquaVerity
Method5-6 sensors5-20 live organismsOne million luminescent bacteria
Detection spectrum5-6 parametersWide range of contaminants, including unknown types.
Determines toxicityNoYesYes
Discrimination between organic & heavy metals Contaminants
NoNoYes
Detection sensitivity
Medium (depends on the parameter)
HighHigh
ContaminationBoundaries assessment
PartialNoYes, by using the portable detectors
EWS Matrix (2)- Implementation
FactorMulti-ParameterOther BiomonitorsCheckLight’sAquaVerity
Installation & maintenance
Complex due to variability of sensors used
Complex & requires on going human supervision
Very simple to install & maintain. Unattended operation.
Adjustment to changing water environment
ComplexComplexMinimal adjustment needed.
Effective coverage in large water networks
Limited to wide distribution. Depends on parameters mix & complexity
Limited distribution due to complexity & costs
Wide distribution possible due to simple installation, minimal training & maintenance.
On-Going usageMedium – requires skills & training
Complex - Requires skilled personnel & special training
Effort is minimal - reagents replacement once a month.
Overall reliabilityMedium. Depends on parameters used.
Low to Medium. Depends on biota used & environment. conditions
Very high. Includes built-in control mechanism.
Future EnhancementsunknownUnknownUpgrade re-fill kits with enhanced detection capabilities
EWS Matrix (3) - Cost Effectiveness
FactorMulti-ParameterOther BiomonitorsCheckLight’sAquaVerity
Initial capital investment
Low to very highDepending on chosen parameters
Medium to very highMedium
On going costsMedium due to complexity of sensors arrays and baseline build up
High due to the required human supervision
Low due to minimal intervention
Total cost of ownership
Medium to highHighLow to medium
Positioning
Sensitive to a broad
range of
contamination sources
Reliable
Cost effective
Easy to operate
Customer oriented
Unique Selling Points
Broad detection capabilities – range & sensitivity
Customer friendly – easy to install, operate & maintain
Reliable – minimum false positives & negatives
Very cost effective for broad EWS implementation
CCB vs ToxControl
FeatureCheckLightCCB-TOX
MicrolanToxControl
SensitivityVery highLow-medium
Discrimination between organic toxicants and cationic heavy metals
YESNO
RepeatabilityHighLow-medium
Needs adjustment to chlorinated water
NOYES
Measuring cycle5-15 minutes30 minutes
UPS installed & adjustedYESNO
Operational down-time per month~15 minutes16-20 hrs
Required skills for maintenanceLowMedium
Reagents re-fill frequencyOnce in 4 weeksOnce in 7 days
Set up timeShortLong
Confidential – for internal use only
IC50 examples (mg/L)
Tested chemicalCheckLightCCB-TOX
MicrolanToxControl
Arsenic (5)0.097.8
Cadmium (2)0.2420
Chromium (6)0.0715.3
DDT0.057
Mercury0.0140.07
Pentachlorophenol0.060.7
Confidential – for internal use only
ToxControl – Key weaknesses (1)
No discrimination between heavy metals and organic contaminants.
Long measurement cycle – slow response time.
Hundreds of liters per week are required for reference water.
Management software is very limited. No 3rd party integration.
Complex and lengthy reagents preparations.
Customer requires a bio-reactor (which is +30% cost on top) with
special media.
Confidential – for internal use only
ToxControl – Key weaknesses (2)
Down time is too long for on-line system.
Weekly maintenance. Manual cleaning.
Requires large space for installation.
Too expensive (total cost is +50% than other solutions).
Conclusion: not practical as a key element of a real Early Warning
solution where broad deployment including in remote locations is
required.
Confidential – for internal use only