Upload
dipanker-singh
View
237
Download
0
Embed Size (px)
Citation preview
8/8/2019 Ccpp, Ys, Pgi Final
1/23
8/8/2019 Ccpp, Ys, Pgi Final
2/23
Overview
About CCPP
Etiology
Epidemiology
Differential Diagnosis
Preventive measures
Actions to Take
8/8/2019 Ccpp, Ys, Pgi Final
3/23
W
hat is CCPP ?
Bou fila
Extremely Contagious & frequently fatal
Innaive flocks, extensive morbidity, mortality
Enlisted asTYPE- B by OIE
8/8/2019 Ccpp, Ys, Pgi Final
4/23
Etiological agent
M. capricolum sscapripneumoniae(Mycoplasma F-38)
M. mycoides cluster
M. capricolum ss.capricolum
M. mycoides sscapri M. mycoides ssmycoides LC type
M. mycoides ssmycoides SC type
Mycoplasma sp. bovine group 7
8/8/2019 Ccpp, Ys, Pgi Final
5/23
Epidemiological features
Captive wild goats
Sheep & Cattle not affected
Chronic & latent CarriersShed more organisms
Stress, close contact , over crowding,confinement, climate changes,
PPR & Capri pox viruses act as predisposing factor
More outbreaks after heavy rains in India
8/8/2019 Ccpp, Ys, Pgi Final
6/23
Africa
Far East
Middle east
Eastern Europe
Former USSR
Asia (India & Pakistan)
Never reported in North America
Distribution
8/8/2019 Ccpp, Ys, Pgi Final
7/23
Countries involved
Confirmed by
isolationof
mycoplasma
Clinical disease reported or
suspected
Africa Chad, Eritrea, Ethiopia,Kenya, Niger, Sudan, Tunisia,
Uganda
Algeria,Burkina Faso,Benin, Cameroon,
Central African Republic, Djibouti,
Egypt, Libya, Mali, Nigeria, Somali, Zaire
Asia Nepal, Oman, United ArabEmirates,
Turkey,Yemen
Afghanistan,Bangladesh,India, Iran, Iraq,
Israel, Jordan, Kuwait, Lebanon,
Pakistan, Saudi Arabia, Syria
8/8/2019 Ccpp, Ys, Pgi Final
8/23
Prevalence in India
No isolationofMccp
(However, F-38 from cattle with mastitis)
Strong serological evidences
Different states
8/8/2019 Ccpp, Ys, Pgi Final
9/23
Differential Diagnosis
Clinical Picture
High fever, lethargy, anorexia Coughing, labored breathings
Reluctant to move
Nasal discharge
Stringy saliva
Abortion
Death, 7-10 days
8/8/2019 Ccpp, Ys, Pgi Final
10/23
8/8/2019 Ccpp, Ys, Pgi Final
11/23
Post Mortem Lesions
Lungs & Pleura(usually unilateral)
Granular lungappearance
Fibrinous pneumonia
8/8/2019 Ccpp, Ys, Pgi Final
12/23
O
ther Lesions
Straw-colored fluid in thorax
Pea-sized yellow nodules
Adhesions tochest wall
Thickened pulmonary pleura
8/8/2019 Ccpp, Ys, Pgi Final
13/23
Sampling
Before collecting or sending any samples,theproper authorities should be contacted
Samples should only be sent under secureconditions to authorized laboratories
8/8/2019 Ccpp, Ys, Pgi Final
14/23
8/8/2019 Ccpp, Ys, Pgi Final
15/23
Molecular techniques
PCR based on 16S rRNA
detects allM. mycoides cluster
Sequencing & REA of PCR productswith PstI detectsM. ccp specifically
PCRcan detectMccp directlyfrom pleural fluid or lung and
also from pleural fluiddried on filter paper
8/8/2019 Ccpp, Ys, Pgi Final
16/23
Criteria for CCPP
IsolationofM. c. capripneumoniae
or strong serological evidence ordetection by PCR-REA
Lesions restricted to lung and pleuraconsisting a pleuropneumonia
Highly contagious with highmorbidity/mortality
No enlargement of interlobular
septa of lung
8/8/2019 Ccpp, Ys, Pgi Final
17/23
Preventive measures
Recommended Actions
Notificationof Authorities Area Veterinarian In-Charge
State Veterinary Authority
Testing,slaughter
Quarantine Desirable for infected flocks
Strict, due to communicability
Disinfection
8/8/2019 Ccpp, Ys, Pgi Final
18/23
Antibiotic therapy
Tetracycline, tylosin,erythromycin, streptomycin
Early intervention and treatment needed
Newly infected countries Trade, movement restrictions
Slaughter of infected animals
8/8/2019 Ccpp, Ys, Pgi Final
19/23
Vaccination
Saponin killed Vaccines available in NVI,
Ethiopia and KVVPI, Kenya Good/excellent protection
Kids above 6 months
0.2 ml I/D, Jan March
Inactivate Mccp just beforeinoculation or 12 h at 40C
8/8/2019 Ccpp, Ys, Pgi Final
20/23
Actions to take
Establishment of diagnostic lab at national level
Notification to higher authorities before collection &sending clinical samples
Sent sample under strict secure conditions & lowtemperature to authorized lab
Differentiate CCPP from Pasteurellosis &M.m.capriorM. mycoides LC infections
Use LAT withMccp for herd diagnosis
8/8/2019 Ccpp, Ys, Pgi Final
21/23
Use PCR based on 16S rRNA with REA to detectMccp.
directly from mixed cultures & clinical samples
It can also detectMccp. directly from pleural fluid dried
on filter paper
In endemic areas, do flocktesting,slaughter,on-site
quarantine of affected and vaccinationof healthy
animals before introducing new animals.
Competitive-ELISA being more specific test mustbe available commercially
8/8/2019 Ccpp, Ys, Pgi Final
22/23
In an outbreak, flock testing followed by quarantine,
movement restrictions, slaughter of affected & contact animals,cleaning & disinfection of premises along with vaccination in
association with antibiotic therapy are better option than
antibiotic therapy alone.
Improvement of both production capacity and technology
needs for saponin inactivated CCPP vaccine used in Kenya &
Ethiopia.
Mccp screening as well as vaccination should be done
before wild goats released into zoos & other places.
8/8/2019 Ccpp, Ys, Pgi Final
23/23