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CECT 434 Escherichia coli(Migula 1895) Castellani and Chalmers 1919
Other culture collections: ATCC 25922; CCM 3954; CCRC 14902; CCTM La 2184; CCUG 7736; CCUG 17620; CCUG 21456; CIP 76.24; CNCTC Ec 327/73; DSM 1103; FDA Seattle 1946; GISK 240533; HER 1176; IFO 15034; JCM 5491; LMG 8223; NCIMB 12210; PCM 2057; WDCM 00013
History: CECT, 1978 < ATCC < FDA < F. SchoenknechtIsolation source: Clinical isolate
Applications: Reference Strain recommended to be used for UNE-CEN ISO/TS 11133, EN ISO 6888 and ISO 15214
Risk group: 2Culture conditions (medium, incubation temperature, incubation time): 1, 37ºC, 24h
CHARACTERIZATION
Miniaturized biochemical test API 20E
Growth on selective/differential culture media
CECT Certified Strains have been selected as typical representatives of species commonly used as reference material after testing that their biochemical profile and their colony appearance on selective/ differential media conform to the expected results.
ENDO AGAR
Red colonies, red medium, golden sheen
TERGITOL AGAR
Yellow colonies with yellow zone. Sometimes with rust coloured centre
+ - + + - - - - + - - + + - + + - + - + -
1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4 1 2 4
ONPG ADH LDC ODC CIT H2S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX
5 1 4 4 5 5 2
Identification: 99,9% ID Escherichia coli
C E R T I F I E D
C E R T I F I C A D A
20121024
TBX (Tryptone Bile X-glucuronide) AGARBlue-green colonies
Page 1 of 2
CECT 434 Escherichia coli(Migula 1895) Castellani and Chalmers 1919
Growth on selective/differential culture media
C E R T I F I E D
C E R T I F I C A D A
CTSM (Sorbitol MacConkeyagar with Cefixime Tellurite) AGARPink colonies
VRBL (Violet Red Bile Lactose Agar) AGAR
Red-purple colonies with or without halos
VRBGA (Violet Red Bile Glucose Agar) AGAR
Red-purple colonies surrounded by purple haloes
20121024Page 2 of 2
MYP (Mannitol Egg YolkPolymyxin) AGARGrowth inhibition
Left: Brilliant Green Bile Lactose BrothRight: Laurylsulfate Tryptose BrothBoth: Turbidity with gas production
EC Broth
Turbidity with gas production