Cell Bio Midterm Hitlist

8/7/2019 Cell Bio Midterm Hitlist

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EM Cell Type Lab Identify:

EM1 pancreas III rough ER

zymogen granules

euchromatin

heterochromatin

nucleus and nucleolus

mitochondria

acinus and lumenintercellular space

extracellular space

golgi

EM2 liver (3 hepatocytes) III mitochondria

golgi

RER

glycogen

autophagosomes/autophagolysomes

peroxisomes

outer mitochondrial membraneinner mito memb and cristae

mitochondrial matrix

VII Gap Junction

intermembrane space

IV lipid droplets

EM 2a Freeze fracture of gap jun VII Gap Junction

EM2b liver, cytoskeletal element IV microtubule

microfilament

intermediate filament

V heterochromatinnucleolus

EM3 intestinal absorptive cell ( II basement membrane (collagen)

basal lamina

mitochondria

interdigitations of plasma membranes of a

EM4 intestinal absorptive and II microvilli and brush border

(apical end) glycocalyx

absorptive cell

goblet cell (mucus droplets)

IV terminal web

chylomicrons

secretory granules

microfilaments

VII Zonula Adherens

Zonula Occludens

Terminal web

EM 4a Freeze fracture of tight ju VII tight junction

EM5 Nucleus (liver) w/nuclear V Nulceus

Nuclear Pores


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heterochromatin

outer membrane of nuclear envelope

inner membrane of nuclear envelope

periplasmic space

ribosomes on outer membrane

EM 6 Nucleus-freeze fracture V Outer nuclear membrane

inner nuclear membrane

nuclear poresEM6a insect spermatocyte IV centrioles

centrioles and microtubules microtubules

EM6b monkey oviduct IV axoneme of cilia (doublet and singlet MTs)

cilia and basal bodies in crosssecti basal bodies

VII Desmosome

Zonula Adherens

Zonula Occludens

EM6c monkey oviduct IV doublets in cilia continous with basal bodie

cilia and basal bodies in longitudinal

EM7 Ascites fluid cell III golgi and vesciclesmitochondria

nucleus

Lysosomes

RER

EM 8a Bone Marrow VI Erythrocyte

Reticulocyte

Eosinophil precursor

Neutrophil

XIII polychromatophilic/orthochromatic erythro

nurse celleosinophilic precursor (myelocyte)

EM 8c Basophil VI basophil

EM8d Mast Cell VIII Mast Cell Granule

EM 8e Fibroblast and Collagen VIII Fibroblast cell

Collagen Fiber

EM 8f Peripheral Blood VI Lymphocytes

Neutrophils

Monocytes

Azurophilic granules

Neutrophil specific granules

Eosinophil

EM 9 Smooth muscle IX Elastin

Endothelium

Lumen

Calveolae Intracellulares (Vesicles)

Myofilaments

Mitochondria

Lysosomes

Rough ER


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Dense Bodies

XI Lumen

Smooth Muscle Nucleus

Elastin

Endothelial Cell Nucleus

Endothelial Cell Junction

EM 10 skeletal muscle IX Triads

H-BandsI-Bands

A-Bands

Z-Line

Mitochondria

Calsequestrin

T-Tubule

Terminal Cisternae

Sarcomere

EM 10a capillary XI Capillary

Junctional Complex (containing Fascia adheVII Lumen

Endothelial cell cytoplasm

Cell-cell junction

Basal lamina

Vesicles

EM10b cardiac muscle III mitochondria

IX Cardiac Myocytes

Fascia Adherens

Desmosomes

SarcomereA-Band

I-Band

H-Band

Z-Line

Diad

T-Tubule

Terminal Cisternae

Gap Junction

EM 10c nerve cell body X Auerbach Plexus

Neuronal Cell Cytoplasm

Neuronal Cell nucleus

Axon Terminals

Synapse

Enteric Glial Cell Cytoplasm

EM10d Schwann cell III golgi

IV lipid droplets

VIII Collagen Type 1

Collagen Type IV

X Schwan Cells


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Myelinating Schwann Cell

Non-Myelinating Schwann Cell

myelinated axon

unmyelinated axon

Myelin

Epineurium

Perineurium

EndoneuriumEM 10e Smooth muscle IX Dense Bodies

Calveolae Intracellulares (Vesicles)

Collagen Fibers

Nerve Ending

External Lamina

Myofilaments

Mitochondria

X Smooth Muscle Neuromuscular Junction

Smooth Muscle

Presynaptic TerminalMitochondria

Vesicles

Synaptic Cleft

EM11 perichondrium XII chondroblasts

perichondrial cell

chondrocyte

type I collagen

type II collagen

EM12 osteoblasts XII osteoblasts

rough ERgolgi

collagen fibrils

hydroxyapatite crystals

cellular projections in canaliculi

EM12a bone marrow XIII nurse cell

erythrocyte progenitors

EM13 chondrocytes XII newly synthesized matrix

rough ER

mitochondria, nucleus, golgi

EM15 Parietal cell (stomach) VIII nucleolus

EM17 duodenum XI Pericyte

Endothelial Cell

Endothelial cytoplasm

Endothelial Nucleus

Basal Lamina

Pericyte Process

EM20 Leydig cell III mitochondria

IV lipofuscin granules

EM24 corpus luteum IV lipid droplets


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EM25 ovary IV crystalloid inclusions

EM27 rat intestines III golgi and vescicles


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What to look for:

highly osmophilic; condensing granules are lighter than mature granules

big gap

dark staining granules slightly larger than ribosomes (rosette-like aggregates)

osmiophilic crystalloid core

region where membranes come together but do not fuse

area where lots of holes are congregated.

outer cytoplasm, underneath plasma membrane; criss-crossing threads

osmiophilic, lines nuclear laminadense core of the nucleus

fuzzy white stuff

fuzzy gray line immediately subjacent to plasma membrane on basal end

jacent cells

composed of microfilaments

top fuzzy gray coat

composed of microfilaments; organelles excluded from this region

membrane-bound clusters

homogeneously staining inclusions

compose microvilli

cytoplasmic area beneath plasma membrane. No organelles hang out here

honeycomb patterned fine lines

where the periplasmic space disappears


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space between the inner and outer nuclear envelopes

little dots decorating the outer nuclear membrane

the bottom of the crater

space under the hole of the outer nuclear membrane

look live rivet holes on the outer nuclear membrane

nine sets of triplet MTs

Darker staining defined area of intercellular plasma membrane come together, about the

area above desmosome, looks like there is space between the intercelluar membranes

darker line in plasma membrane junctions, just apical to zonula occludens

s

the most osmiophilic solid, amorphous blob, no organelles

the 2nd most osmiophilic solid, amorphous blob, no organelles

large, regular granules that are osmiophilic

next to the developing RBCs

lasts

electron-dense granules containing debris from discarded nuclear contents of differentiatiextensive rER and specific granules

only thing in the EM. Bilobed nucleus, chock-full of dark homogenous granules

the only thing in the cytoplasm

cross section. A grouping of dots extrecellularly

no granules, large amourphous nucleus

Multi-lobed nuclei, granules of varying osmophilicity

no granuels, horseshoe or lobed nucleus that is much smaller than lymphocites

the largest granules

contain what looks like highly osmiophilic equal signs, '='

Granules are large, dark and homogenous

Located between Smooth muscle cell and endot Located in between Sarcomeres, Use Cal

Surrounds lumen Located in Center of Sarcomere Just outsi

Light bands Anchored to Z-Line

Dark Bands containing H bands

Located within Smooth Muscle Cell Dark Line Perpendicular to I bands

Identify by Cristae round organelles located between muscl

Dark spots used to identify Triad

Identify by Ribosomes Located inbetween Calsequestrin protein


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Appear as thickened bands in cell membrane ligher round tubules continuous with SR

Upper Right hand Corner Z-line to Z-line

Identify by recognizing smooth muscle fibers surrounding nucleus and pill shaped nucleus

Located between smooth muscle and endothelial cell

irregular shape nucleus outlined in heterochrom Dense staining filament with light stainin

dense line located between 2 endothelial cells Dense staining filament with light stainin

predominant due to secretory activityLight staining band near Z-Line

note tubular cristae (tubulovesicular in tr

Dark band Perpendicular to thin an thick filamenirregularly shaped, dense deposits

dark staining part

light staining part

Dense bands between adjacent endothelial cells

where membranes of the two endothelial cells meet

the fine dark line that borders the outside of the endothelial cells

Located within Endothelial Cells

\

Rough ER, free polysomes and multiple mitochondria

Bottom of EM, Surrounded by heterochromatin,

Contain multiple synaptic vesicles

Dark line near axon terminal

contain densely packed intermediate filaments

Series of dots in cross section, longitudinall a fine line floating in the ECM

along the extermal lamina of the schwann cell


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Large Nucleus next to myelinated axon

cytoplasm surrounds axon terminals at top of EM

Axon surrounded by dark gray ring

axons located top of EM

Dark Gray ring around axon

bottom left corner of EM

contains nucleus at bottom of EM

Connective tissue visible between Schwan Cell and Nucleus of Perineurium

Continuous Border surrounding

Dark Gray longitudinal fibers Nucleus bulges into Lumen

Lightly stained ring, filled with vesicles, contains surround Capillary Lumenlocated in presynaptic terminal 2

located in presynaptic terminal

space between smooth muscle and presynaptic terminal

differentiate phagolysosomes and mitochondria


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size of a basal body

ng erythrocytes

equestrin to Identify

de M-Line

fibers


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strip down the center

strip down the center

nsverse)


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Pericyte 11 Nucleus d EM17

Basal Lam 11 Continuous Border s

Endotheli 11 Nucleus bulges into

Pericyte P 11 surround Capillary L


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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus d EM17

Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border s

Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into

Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary L


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Pericyte 11 Nucleus d EM17 Pericyte 11 Nucleus does not bul

Basal Lam 11 Continuous Border s Basal Lam 11 Continuous Border su

Endotheli 11 Nucleus bulges into Endotheli 11 Nucleus bulges into L

Pericyte P 11 surround Capillary L Pericyte P 11 surround Capillary Lu


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e into Lumen

rounding

men

en


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term Exam: Slide Hitlist

Slide Tissue Type: Lab

102 skin V

104 thumb skin XIV

105 Finger skin VII

XIV

107 idermal sheet, DOPA reacti XIV

108 Scalp Skin VIII

XIV

109 skin (monkey) X

XI

V

110 peripheral blood smear I & VI


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111 Forearm Skin VII

XIV

112 bone marrow aspirate XIII

May-Grunwald-Giemsa stain

113 peripheral blood smear VI

121 bone (rabbit femur) XII

XIII

122 Spleen VIII

125 heart IX


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126 bile duct with portal vein XI

(monkey)

128 thoracic duct XI

130 submandibular gland X

VII

133 trachea (monkey) X

XI

VII

VIII

136 tongue (monkey) IX


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XI

137 esophagus III

IX

140 Infected Cardiac Stomach VI

146 duodenum (monkey) IX

VIII

147 pyloroduodenal junction IX(baboon)

148 ileum II

V

VI

VIII

X

149 mall intestine (mouse, PAS II

VII

152 duodenum X


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155 ll bladder and liver (monke XI

V

156 creas (monkey, toluidine bl III

157 pancreas (monkey) I

V

158 pancreas III

X

160 urinary bladder (monkey) IX

VII179 fallopian tube IV

VII

182 Lactating Mammary Gland VII

186 adrenal XI

193 peripheral nerve (monkey) X

X

194 fetal elbow XII

195 fetal jaw XII


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196 Vessels and Endothelium VII

199 epididymis XI

202 Tendon VIII

205 idermal sheet, DOPA reacti XIV

206 abdominal skin VIII

XIV

207 skin, abdominal (Gallego's) XIV

208 newborn skin XIV

208 skin (newborn) III

VIIIXI

209 scalp XIV

210 skeletal muscle IX

Masson's trichrome stain

X


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XI

211 skeletal muscle IX

IX

220 fetal finger I

XII

225 heart (epicardium) IX

226 heart (endocardium) IX

227 lastic and muscular arterie XI

elastin stain

231 Blood Smear, Leukemia VI

XIII

401 icine and Cytochalasin Cult V

405 skeletal muscle and tumor IV

desmin stain


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IX

406 ooth muscle (intestine, PA IX

407 skeletal muscle and tendon IX(guinea pig)

408 sole of foot (skin) XIV

420 rib XI

XII

XIII

421 tibia XII

422 bone (femur, monkey) XII

XIII

425 bone (unstained) XII


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426 al artery and vein with ner X

XI

427 aorta XI

432 Lung VIII

439 salivary gland IX(stained for smooth muscle actin)

447 denum (monkey, toluidine b II

VIII

XI

472 fallopian tube IV

480 Vagina VII

481 reast Tumor (toludine blue VIII

492 eripheral ganglion (monkey X


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Identify:

Interphase Cell

Prophase Cell

Metaphase Cell

Anaphase Cell

Telophase CellCell in Cytokinesis

epidermis

stratum corneum

Meissner's corpuscles

single, unbranched duct

epidermis

dermis

subdermis

duct of sweat gland

secretory portion of sweat glandPacinian corpuscles

melanocyte

epidermis

dermis

collagen

subdermis

fibrolasts

white adipose tissue

melanocyte

Langerhans cellhair follicle

external root sheath

internal rooth sheath

sebaceous gland

dermal papillae

differentiation zone

arrector pili muscle

Pacinian Corpuscles

Small Artery

Small Veins

Arteriole

Venule

Capillary

Viable Keratinacyte

RBC's

Neutrophil

Lymphocyte

Monocyte

Platelet


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Intercalated Discs

accumulated lipofuscin granules

Cystic Duct

Bile Duct

Portal vein

Hepatic Artery

Layers of Hepatic Atery

Small ArterySmall Vein

Arteriole

Arteriole (longitudinal)

Venule

Capillary

Lymphatic Vessels

Thoracic Duct

Lumen

Intima

MediaAdventitia

Endoneurium

myelinated axons

unmyelinated axons

Capillaries

Perineurium

Epineurium

serous gland cells

mucin secreting cells

ductEndoneurium

myelinated axons

unmyelinated axons

Capillaries

Perineurium

Epineurium

Small Artery

Small Vein

Arteriole

Venule

Capillary

pseudostratified epithelium

brown adipose tissue

Gland

Capillaries

A Band

I Band

Z Line

Epithelium


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Fat

Fascicle Cross section

Fascicle longitudinal

Perimysium

Capillary

folds and wrinkles

Skeletal Muscle Transverse

Skeletal Muscle LongitudinalSmooth Muscle Transverse

Smooth Muscle Longitudinal

WBCs

Collagenous Connective Tissue

Smooth Muscle longitudinal

Smooth Muscle Transverse

Mast Cell

Macrophage

plasma cell

Smooth Muscle TransverseSmooth Muscle Longitudinal


epithelial cells lining villi and their nuclei

goblet cells lining villi

long thin nuclei in absorptive cells

apical vs basal end

brush border

microvilli

Paneth Cells

NeutrophilEosinophil

connective tissue

Mast Cell

Macrophage

Auerbach Plexus

Neuronal Cell Bodies

Enteric Glial Cells

Meissner's Plexus


Enteric Glial Cells

carbohydrates

simple columnar epithelium

Auerbach Plexus


Enteric Glial Cells

Meissner's Plexus


transverse sections of villi: epithelial cells (absorptiveand goblet cells), basement membrane


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Enteric Glial Cells

Sinusoids

Binucleated cells

acinar cells

acinus, acinar cell, and nucleus (100x) with heterochr

basophilic nucleus

basophilic basal (lower) cytoplasm (RNA)

acidophilic apical cytoplasm (secretory granules)heterochromatin

pancreatic acinar cell and nucleus

acidophilic granules in apical cytoplasm

Peripheral Nerve tissue

Collagen

Smooth Muscle


Smooth Muscle longitudinal

Smooth Muscle Transverse

transitional epitheliummotile cilia

basal bodies

simple columnar epithelium

basal bodies

alveoli

Sinusoids

Longitudinal Section

Endoneurium

Perineurium

EpineuriumMast Cell

Schwann Cell Nuclei

Node of Ranvier

Schmidt-Lanternman cleft

Transverse Section

Endoneurium

myelinated axons

unmyelinated axons

Capillaries

Perineurium

Epineurium

hyaline cartilage

perichondrium (contains perichondrial cells)

chondrocytes, condroblasts, lacunae

bone

vascularized mesnechymal tissue

osteoblasts

osteocytes

osteoclasts


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simple squamous epithelium

Lymphatics

Lumen

Intima

Media

Adventitia

Collagen

fibroblastsmelanocyte

Macrophage

epidermis

dermis

subdermis

dermal-epidermal junction

dermal papillae

epidermal pegs

sweat gland duct

capillariescollagen fibers

sweat gland (secretory)

adipose cells, blood vessels and nerves of subdermis

melanin within keratinocytes

Langerhans cells

melanocyte

review skin components

review skin components

empty spaces (formerly fat droplets)

white adipose tissueMuscular Artery

Small Vein

hair follicle

external root sheath

internal rooth sheath

dermal sheath

sebaceous gland

dermal papillae

differentiation zone

arrector pili muscle

Epimysiusm

Perimysium

Fascicles

Muscle Spindle

Intrafusal Fibers

Extrafusal Fibers

Nerve tissue

Perineurium

Collagen of Epimysium


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Capillaries

Muscular Artery

Small Vein

Transverse Section

Skeletal Muscle Fibers

Endomysium

Longitudinal Section

NucleiEndomysium

A Band

I Band

Z Line

Sarcomere

acidophilic areas (proteins)

basophilic areas (sulfates)

hyaline cartilage

perichondrium (contains perichondrial cells)

chondrocytes, chondroblasts, lacunaespongy bone

trabeculae (w/in spongy bone)

bone marrow/hemapoetic tissue

calcified cartilage

osteoclasts

osteoblasts

osteocytes

epiphyseal region

endochondral ossification

EpicardiumMyocardium

Visceral Pericardium

Intercalated Discs

Coronary Vessels

lipofuscin granules

Myocardium

Endocardium

Purkinje Fibers

Smooth Muscle

Endothelial Cells

aorta

Aortic Elastic Lamina

Muscular Artery

WBCs

precursors to eosinophils and basphils (see hit list for

Cells treated with Colchicine

Cells treated with Cytochalasin

positive (brown) stain in skeletal muscle

brown stain in tumor cells


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Z-line

Muscle Fibers

Nuclei

Smooth Muscle Cell Nuclei Longitudinal

External Lamina Longitudinal

Contracted smooth muscle

External Lamina Transverse

Skeletal MuscleTendons

epidermis

stratum corneum

epidermal pegs

Muscular Artery

Small Vein

trabeculae

bone marrow

osteoblasts

osteoclastslacunae and osteocytes

canaliculi

bone marrow

megakaryocytes

adipocytes

spongy bone

calcified cartilage

trabeculae (w/in spongy bone)

hematopoetic tissue

osteocyteszone of proliferation

zone of hypertrophy

zone of calcification

zone of bone deposition and remodeling

osteoblasts

periosteum

outer circumferential lamellae

medullary cavity

adipocytes

bony trabeculae

bone marrow

Sinusoids

cells of extravascular compartment

megakaryocytes

osteon/Haversian system

Haversian canal

concentric lamellae

osteocytes, lacunae

canaliculi


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Look for:

quiescent, intact membrane with multiple nuclei

condensed chromosomes in a haphazard array. Mitotic spindle formation

fully condensed chromosome centered in the middle plane. From single line for "floral" arr

two chromosomal groups separated by microtubule procceses

decondensation of chromosomes, formation of nuclear envelopenuclear envelopes formed but cytoplasm still attached

thickened

prominent, with deep epidermal pegs

in dermal papillae, recognized by pattern of Schwann cells

a continuaion of epidermal cells inward to form a tubular duct in the dermis, running towar

stratified, cuboidal epithelium, 2 layers

pseudostratified epithelium containing clear cells and dark cells (secretory granules)deep dermis and subdermis, concentric lamellar structure

stellate appearance; note hill and valley appearance of epidermis

dark staining, most superficial layer

subjeacent to dermis, dense connective tissue

eosinophilic, in areas that are devoid of cells

loose connective tissue, beneath dermis, lots of adipocytes in the area

in connective tissue, plump cells with oval nuclei with 1 prominent nucleolus or can be elon

where the large holes are, have huge lipid droplet, little cytoplasm and a flattened nucleus

clear cell in basal cell layer

clear, stellate cell in spinous layer; may be able to see processescontinuous with epidermis

group of cells adjacent to follicles

smooth muscle that connect lower half of root sheaths with upper portion of dermis

light eosinophilic stain, concentric, lamellar appearance

just underneath the basophilic, superficial keratinized layer

acidophilic, note size

highly segmented nucleus and pale cytoplasm

large, round nucleus, heavily condensed chromatin, thin rim of light blue cytoplasm

Largest cell in peripheral blood, 3X RBC, large kidney-shaped nucleus

anucleate bodies in clumps, look like specks


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thin magenta line directly under epithelia

darker staining region at the apical end of the boundaries between cells, very subtle in ligh

observe the multi-layered basophilic epidermis with thin outer layer noting the change in s

at 100X note the artifactual spaces between cells that remain attached at points by desmo

attached by hemidesmosomes to the dermis

spines or prickles represent desmosomal attachments

one or two layers of darkly staining keratohyalin granulescompletely keratinized, exfoliating cells

pale cells in basal cell layer that appear unconnected to adjacent cells and often protrude s

avoid densely packed areas; areas around adipocytes are most representative of cell popul

large cells; multilobe nucleus; purple-staining cytoplasm due to abundant granules; should

large phatocytotic cells with long processes and normally attached to reticular fibers; in pre

large cell with large, centrally located nucleus; nuclear chromatin is finely dispersed, giving

nuclear condensation, blue cytoplasm (know distinction from proerythroblast)

more chromatin condensation; variable cytoplasm color (gray or pink-blue); smaller than pr

nucleus is pyknotic mass, ready to be extruded; cytoplasm looks like that of reticulocyteno nucleus; light gray cytoplasm; larger than mature erythrocyte

oval, eccentrically-placed nucleus containing finely dispersed chromatin; blue cytoplasm wi

increasingly pink cytoplasm with blue in later stages

cytoplasm is color of mature neutrophil (no basophilic ER present); nucleus has minimal clu

nucleus has condensed and become horse-shoe shaped

little nuclear condensation and eosinophilic granules

most abundant, eosiniphilic

tri-lobed nucleus and very mildly eosinophilic cytoplasm

bi-lobed nucleus, bright red granuleslarge, round nucleus, heavily condensed chromatin, thin rim of light blue cytoplasm

Largest cell in peripheral blood, 3X RBC, large kidney-shaped nucleus

anucleate bodies in clumps, look like specks

lines haversion canals, bone trabeculae, and compact bone surrounding marrow cavity

first few layers of compact bone next to endosteum

surrounded by concentric circles of lamellae, containing osteocytes

multiple layers of osteoprogenitor cells and connective tissue outside the bone

may see many artifacts where lipid droplets have been extracted

homogenous eosinophilic stain

large, thin-walled vessels lined by endothelial cells and filled with mature blood cells, prima

large cells with multi-lobded nuclei that have easinophilic cytoplasm\

eosiniphilc band running down a side of the tissue sample. Composed of dense connective

Centrally located nuclei with branched fibers, notice fibers consists of multiple myocytes


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slightly brighter stained band between cardiomyocytes

Smallest lumen, lined by columnar epithelia

located between Portal vein and Cystic Duct, lined by columnar epithelia

largest lumen

2nd smallest lumen

From Lumen: Endothelium, Internal Elastic Lamina, Media, Adventitia

Media containing up to 8 layers of smooth muscle, stains brighter, small lumen

Media containing 1-2 layers of smooth muscle

only a few nuclei surrounding lumen

homogeneously stained lymph

homogeneously stained lymph

thin layer of endothelia

spiral smooth muscleconnective tissue and vasa vasorum in adventitia

Eosinophilic contains axons,myelinating/unmyelinating Schwann cells, loose connectivie tis

dark staining rings in endoneurium

pale specks in endoneurium around myelinated axons

Appear as empty space in endoneurium

Thin layer of cells surrounding endoneurium, Notice absense of axons

Mostly translucent space with interspersed thick sheaths of connective tissue surrounding f

contain zymogen granuales and arranged in simple acini

scattered among serous cells, flat nuclei along the basal membrane, mucin vesicles give cy

Eosinophillic contains axons,myelinating/unmyelinating Schwann cells, loose connectivie ti






Irregularly shaped lumen

Irregularly shaped lumen, thin wall

note how every cell in the tracheal lining is attached to the basememnt membrane not not

adipocytes have a central, round nucleus, and cytoplasm has many small lipid droplets. Dr

bringhtly stained pink

Appear as gaps in muscle fibers, be sure to see endothelial cells to confirm not an artifact

Strongest Staining with Hematoxylin

light staining with hematoxylin

second strongest staining with hematoxylin

brightly stained pink at edge of tissue


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Clear Artifacts

Eosinophilic, multinucleated cannot see strations

Eosinophilic, multinucleated can see striations

thin border aroudn fascicle, easier to see on cross section sample

Use endothelial cell to differentiate between capillary and gap

artifacts

Nuclei are peripherally located, appear more uniformily stained

Nuclei are peripherally located, appear more uniformily stainedNuclei are centrally located, more densly nucleated

Nuclei are centrally located, more densly nucleated

look in vessels, ECM connective tissue. Note the proliferation of WBCs mediating the infla

Nuclei located in the periphery of cells, stain brighter pink

Long and thin cells and nuclei, Nuclei are centrally located and more densly packed than fi

small diameter, due to transverse section nuclei may appear to be missing

look in lamina propria, round, central nucleus with brightly staining granules

voluminous, heterogeneous cytoplasm, large cells with irregular shape in the connective ti

dark staining cytoplasm (basophilic) and cartwheel nucleus, located in lamina propria

small diameter, due to transverse section nuclei may appear to be missingLong and thin cells and nuclei, Nuclei are centrally located and more densly packed than fi


be able to identify different images created by sectioning at different angles

darkly staining mucus in cytoplasm; secreting cells will show gray mucus

purple stain

apical end faces the lumen; acidophilic basement membrane is thin pink line beneath epith

thick acidophilic band on apical surface

at the base of crypts, have bright red granules that contain lysozyme

in large vessels and ECM, tri-lobed nucleus and very mildly eosinophilic cytoplasmin large vessels and ECM, bi-lobed nucleus, bright red granules

note differences in the connective tissue in the lamina propria vs. the submucosa, loose vs.

ln connective tissue beneath villi, round basophilic nucleus, eosiniphilic granules. Don't mi


Located between Circular and Longitudinal smooth muscle layers of intestine

Cell nuclei are large and pale, round or oval in shape and eccentrically placed

Nuclei smaller than Neuronal Cell bodies

Found in Submucosa (dense connective tissue) near border w/ circular smooth muscle

Larger than neuronal cell bodies in Auerbach plexus

Smaller than glial cells in Auerbach plexus

bright pink

brush border (glycocalyx of apical memb) and mucus droplets of goblet cells are PAS+; bas

single, columnar layer of cells all attached to the basement membrane

Located between Circular and Longitudinal smooth muscle layers of intestine

Cell nuclei are large and pale, round or oval in shape and eccentrically placed

Nuclei smaller than Neuronal Cell bodies

Found in Submucosa (dense connective tissue) near border w/ circular smooth muscle

Larger than neuronal cell bodies in Auerbach plexus


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Smaller than glial cells in Auerbach plexus

Irregular shaped capillaries, filled with erythrocytes

cells with 2 nuclei. Just trying to prove that they do naturally exist.

light cytoplasm and blue zymogen granules

matin and euchromatin, nucleoli

acidophilia is pink (eosin) and basophilia is blue (hematoxylin)

condensed basophilic material along the edge of the nuclear envelope

pyrimidal and arranged in clusters (acini); nucleus usu in basal (peripheral) part of cell

stain less intensely than smooth muscle, wavy bundle near blood vessels

haveperipherally located nuclei and more brightly stained

Centrally located nuclei


Long and thin cells and nuclei, Nuclei are centrally located and more densly packed than fi

small diameter, due to transverse section nuclei may appear to be missing

deep cells in epithelium are cuboidal or columnar and superficial cells have convex surface

intense staining at top of cytoplasm

single, columnar layer of cells all attached to the basement membrane

thinly staining line along the top edge of the ciliated cells

secretory unit in which the lumen is greater in sze than the individuals cells.

Irregular shaped capillaries, filled with erythrocytes

Eosinophillic most intense staining, dense fibroblast nuclei

Thin layer of cells surrounding endoneurium, less distinct in longitudial section

lightly stained irregularly spaced connective tissue outside of fasciclePeripherally located basophilic nucleus, cytoplasm eosinophilic (due to preparation

Basophilic, pill shaped

Spaces between schwann cells, multiple slits appear to form X

V-shaped slits

Eosinophillic contains axons,myelinating/unmyelinating Schwann cells, loose connectivie ti






homogenous intercellular substance; stains blue-gray to purple b/c of sulfates; collagen not

intermembranous bone formation

bone surface, towards outside

along bone surface, large clear nucleus, large cells


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thin, flat cells with nuclei protruding into the lumen of vessels

eosiniphilic, regular pattern, not many cells in it

basophilic nuceli, in the margins of fiber bundlesstellate appearance; note hill and valley appearance of epidermis

contain brownish-gold granules in the upper part of the dermis. Have phagocytosed melan

dark purple outer layer (thin)

dense, eosinophilic layer

loose eosinophilic layer with many clear regions

"hill and valley" organization; basal region of epidermis brown due to melanin in basal kera

projections of dermis into epidermal layer

w/in epidermal pegs; stratified cuboidal epithelium (2 layers) with close nuclei

in dermis near base of epidermisdermis

tall, pseudostratified epithelium

primarily above nucleus

stellate cells in spinous layer with clear halo around nucleus (artifact)

clear cells in basal cell layer

where the large holes are, have huge lipid droplet, little cytoplasm and a flattened nucleus

Thick media

Thin MediaStains blue, connective tissue surrounding the muscle

Stains blue surrounds fascicles

group of cells adjacent to follicles

innermost sheath of follicle

outermost sheath of hair follicle

blue stain around red group of muscle fibers

Purple ring surrounding a group of fascicles

Fascicles located within muscle spindle

Stains intense blue

muscular media of arteries stain red and is much thicker than small veins


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Eosinophilic, multinucleated cells with nuclei at the periphery

lightly eosinophilic connective tissue between muscle fibers

basophilic cluster at periphery of muscle fiber

clear space between muscle fibers

Dark bandsLight bands

located in the center of the I bands

Muscle fiber unit, z-line to z-line

lined with endosteum, which contains osteoprogenitor cells, osteoblasts, and osteoclastscomposed of trabeculae

eosinophilic

large multinucleate cells with abundant cytoplasm, eosinophilic; fewer in number than oste

basophilic

large multinucleate cells with abundant cytoplasm, eosinophilic; fewer in number than oste

along surface of the trabeculae, large cells with single nucleus and basophilic cytoplasm

trapped in lacunae of trabeculae

large amount of adipose tissuemuscle layer

did not survive processing -- mesothelial cells surrounding the heart

arteries have thicker smooth muscle layers than veins

gold colored, near some nuclei

heart muscle

surface layer of endothelial cells, with connective tissue and smooth muscle cells

thicker than normal cardiomyocytes, do not stain as intensely and have fewer myofibrils

in cross section

surface layer of cells and continouls with endothelium of vasculature of valves

observe thick intima, thick media with a lot of elastin, muscle is yellow and collagen is pink

stains black

internal elastic lamina stained black, thin scattered fibers in media; some fibers in adventiti

observe neutorphils, eosinophils and basophils (dark staining granules) in varying stages o

121)

Microtubules disrupted. Cells have multiple unequal nuclei

microfilaments disrupted; binucleate cells b/c cytokinesis unable to occur

anti-desmin probe

positive stain indicates rhabdomyosarcoma (skeletal muscle tumor)


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Stained dark by anti-desmin

Rust color

Blue located on periphery of muscle fibers

Dark Staining

Magenta stained filaments in smooth muscle cells

Nuclei appear corkscrew in shape

Weblike appearance surrounding Smooth Muscle cells

Use Striations to differentiate from Collagenous TendonBrightly stained pink dense connective tissue (lack of striations

thickened

prominent, with deep epidermal pegs

Thick media

Thin Media

surrounding trabeculae of spongy bone


outer edge of bone, large cells with basophilic cytoplasm

very outer edge of bone, with basophilic cytoplasm

may see many artifacts where lipid droplets have been extracted

homogenous eosinophilic stain

large, thin-walled vessels lined by endothelial cells and filled with mature blood cells, prima



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stain less intensely than smooth muscle, wavy bundle

have centrally located nuclei and more brightly stained

Centrally located nuclei

Thicker Media

larger, Irregular shaped Lumen

Thin,

Contains Longitudinal smooth muscle

bright pink

thick: EC layer plus subendothelial connective tissue

thick, with elastic laminae interspersed with smooth muscle cells

arterioles, cap, and venules in the adventitia

contain black or dark green granules representing residue of material inhaled from the poll

stain brown due to anti-actin stain, located around acinar cellssurrounded by brown staining myoepithelial cells

Look like arterioles with small lumen and no myoepithelial cells

light blue stain

darker blue stain

darker than cytoplasm; basal end of absorptive cells

oval organelles with heterogenous staining; dark areas are nucleoli; dark=heterochromatin

In lower part of the villus among connective tissue, stains dark pruple to black, small and


ln connective tissue, round basophilic nucleus, metachromatic, purple granules.

as opposed to keratinized stratified squamous, the flattened superficial cells are viable and

Basophilic material in cytoplasm of Neuronal Cell Body

nuclei surrounding neuronal cell bodies

Appear empty in the perfusion fixed sample


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ngement

s a coiled sweat gland below

gate cells with long nuclei


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t microscopy

ape from the basal layer to the surface

omes

omewhat beneath the dermal-epidermal junction

ation

be able to stand out at 10x

paration, pulled away from fibers and appear ragged; pale, clear cytoplasm often with gr

it a wispy appearance; deep blue cytoplasm with ribosomes but no granules

ecursors

th very small azurophilic non-specific granules

mping and curved

rily erythrocytes

tissue


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sue

ascicles

toplasm a frothy appearance.

sue

ascicles

all extend to apical surface

oplets are individually extracted, so the cells skeleton is still visable


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atory response

roblast nuclei

sue

roblast nuclei

elial cells

dense

take for an eosiniphil. It does not have a bi-lobed nucleus

sue at tips of villi

ement membrane will be bright pink line


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roblast nuclei

sue

ascicles

seen b/c forms fine fibrils


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in pigment that escaped from the epithelium

tinocytes; projections of epidermis into dermal region called epidermal pegs or rete bege


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oblasts; look for large eosinophilic cell mass with lots of nuclei

oblasts; look for large eosinophilic cell mass with lots of nuclei

ia mixed with collagen

development


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rily erythrocytes


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uted environment.

, light=euchromatin

niform in size

sue

nucleated


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s


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Documents

Cell Bio Midterm Hitlist