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CellsCellsMicrobiology 2314
General Properties of Living General Properties of Living OrganismsOrganisms
• Metabolism
• Growth
• Reproduction
Characteristics of Life?????
• Responsiveness or Irritability
• Conductivity
• Growth
• Respiration
• Digestion
• Metabolism
• Absorption
• Secretion
• Excretion
• Circulation
• Reproduction
Cell TheoryCell Theory• Cells are the fundamental units of life
• Cells are the simplest unit capable of independent existence
• All living things are made of cells
Note: Credit for developing Cell Theory is usually given to two scientists, Theodor Schwann, a zoologist and Matthias Jakob Schleiden, a botanist. In 1839 these two scientists suggested that cells were the basic unit of life. In 1858, Rudolf Virchow concluded that all cells come from pre-existing cells thus completing the theory.
Modern Cell Modern Cell Theory Expands Theory Expands
These Ideas…These Ideas…
• Cells contains hereditary information which is passed from cell to cell during cell division.
• Cells have similar chemical composition, metabolic activities, and physiological functions.
• Cell activity depends on the activities of organelles.
Organizational HierarchyOrganizational Hierarchy
• Biology is hierarchial with each level building on the level below it.
• In order to understand how something is built and how something works, you must look at all of its components and analyze them both individually and together.
Life’s HierarchyLife’s Hierarchy
• Atom / Element
• Molecule / Compound
• Organelle
• Cell
• Tissue
• Organ
• Organ System
• Organism
• Population
• Community
• Ecosystem
• Biosphere
• Atoms form all matter.
• Atoms are the basic building blocks of matter that make up everyday objects. A desk, the air, even you are made up of atoms!
• There are 90 naturally occurring kinds of atoms. Scientists in labs have been able to make about 25 more.
Atom
• Groups of atoms form molecules.
• Molecules come together to form compounds and compounds come together to form organelles
• Organelles can include the nucleus, endoplasmic reticulum, golgi apparatus, mitochondria, etc.
Atom
Organelle
Mitochondria are the 'power plants' of cells that convert organic materials into energy. Mitochondria have their own DNA and may be descended from free-living prokaryotes that were related to Rickettsia bacteria
• The basic unit of life is the cell.
• All living things are composed of one or more cells
• The human body contains about 100 trillion cells.
• There are about 200 different types of cells in the human body.
Atom
Organelle
Cell
• Tissue is a collection of interconnected cells that perform a similar function within an organism.
• The study of tissue is known as histology, or in connection with disease, histopathology
• There are four basic types of tissue in the body of all animals, including the human body and lower multicellular organisms such as insects.
Atom
Organelle
Cell
Tissue
Types of TissuesTypes of Tissues
• Organs are the next level of organization in the body.
• An organ is a structure that contains at least two different types of tissue functioning together for a common purpose.
• There are many different organs in the body: the liver, kidneys, heart, even your skin is an organ.
Atom
Organelle
Cell
Tissue
Organ
• Organ Systems are composed of two or more different organs that work together to provide a common function.
• There are 10 major organ systems in the human body.
Atom
Organelle
Cell
Tissue
Organ
Organ System
• In biology and ecology, an organism is an individual living system (such as animal, plant, fungus or micro-organism).
• In at least some form, all organisms are capable of reacting to stimuli, reproduction, growth and maintenance as a stable whole.
Atom
Organelle
Cell
Tissue
Organ
Organ System
Organism
Albino Peacock
• An organism may be unicellular or made up, like humans, of many billions of cells (multicelluar) divided into specialized tissues and organs.
• Based on cell type, organisms may be divided into the prokaryotic and eukaryotic groups.
• In biology a population is the collection of organisms or individuals of a particular species located in a specific area.
Atom
Organelle
Cell
Tissue
Organ
Organ System
Organism
Population
• A community includes all the biotic (living) organisms sharing an environment, normally with shared interests.
Atom
Organelle
Cell
Tissue
Organ
Organ System
Organism
Population
Community
• An ecosystem is a natural unit consisting of all the biotic and abiotic elements that composes it.
Atom
Organelle
Cell
Tissue
Organ
Organ System
Organism
Population
Community
Ecosystem
• The biosphere is the part of the earth, including air (atmosphere), land (lithosphere), and water (hydrosphere), within which life occurs, and which biotic processes in turn alter or transform.
• From the broadest point of view, the biosphere is the global ecological system integrating all living beings and their relationships.
Atom
Organelle
Cell
Tissue
Organ
Organ System
Organism
Population
Community
Ecosystem
Biosphere
Cells are 90% water. A mere 2% drop in body water can trigger fuzzy
short-term memory, trouble with basic math, and difficulty focusing
on the computer screen.
Cell Elemental CompositionCell Elemental Composition
• 50% Protein
• 15% Carbohydrate
• 15% Nucleic Acid
• 10% Lipid
• 10% Miscellaneous
Cells are 90% water. Of the remaining molecules present, the dry weight is approximately:
• Proteins are found literally everywhere in your system. From your muscle tissues, to the enzymes that digest your food, to your skin cells, and even within your blood.
• When we take protein in our body through the foods we eat, it gets broken down into smaller compounds called amino acids. Of the 20 amino acids found in the foods we eat, 9 of these are essential.
• All carbs end up as sugar. Starches, or complex carbohydrates, are just longer strings of sugar.
• Lipids are among the fundamental categories of nutrients that are required by our bodies for healthy functioning.
“We are a generation that is computer literate but food illiterate.”
Brief History of Life on EarthBrief History of Life on Earth
• 4.5 Billion Years Ago Earth Formed
• 3.5 Billion Years Ago First Life (Prokaryotic)
• 1.5 Billion Years Ago Eukaryotic Cells Arise
• 0.5 Billion Years Ago Multicellular Eukaryotes
Comparing Prokaryotic and Comparing Prokaryotic and Eukaryotic CellsEukaryotic Cells
Similarities Between the TwoSimilarities Between the Two
• Chemical Composition
• Chemical Reactions
• DNA
• Membrane Bound
• Ribosomes
• Basic Metabolism
• Occur in Diverse Forms
Differences Between the TwoDifferences Between the Two
• Procaryotic cells lack membrane-bound organelles (Including a Nucleus)
• Procaryotic cell walls contain peptidoglycan
Classifying LifeClassifying Life
Life
/ \
Prokaryotes Eukaryotes
/ \ |
Archeabacteria Eubacteria Eukarya (Ancient Bacteria) (True Bacteria) (Everything Else)
Two Groups of ProkaryotesTwo Groups of Prokaryotes
• Archaebacteria
1. Methanogens
2. Halophiles
3. Hyperthermophiles
4. Thermoplasma
• Eubacteria
1. Cyanobacteria
2. Soil Bacteria
3. Nitrogen-Fixing
4. Pathogens
EubacteriaEubacteria
• More Well Known• Can be Pathogenic
ArchaebacteriaArchaebacteria
• Ancient• Non-Pathogenic• Extreme
Environments• Unusual Metabolizing
Abilities• No Peptidoglycan• Resistant to Lysozyme
The hot springs of Yellowstone National Park, USA, were among the first place Archaea were discovered
MethanogensMethanogensAnaerobic Methane
Producers
• Common in wetlands
• Responsible for marsh gas
• Found buried under km of ice in greenland and under dry deserts.
• They are known to be the most common archaebacteria in deep subteranean habitats.
Extreme HalophilesExtreme Halophiles
Salt-Dependent Organisms
Anywhere with a concentration of salt 5 times greater than the salt concentration of the ocean
•The Great Salt Lake, Utah •Owens Lake, California •The Dead Sea
HyperthermophilesHyperthermophilesHeat-Dependent
Organisms
Hyperthermophiles were first discovered in the 1960s in hot springs in Yellowstone National Park, Wyoming.
The most hardy hyperthermophiles thus known live on the superheated walls of deep-sea hydrothermal vents, requiring temperatures of at least 90 °C for survival.
Hyperthermophiles produce some of the bright colors of Grand Prismatic Springs
ThermoplasmaThermoplasma
Heat and Acid
Resistant
Example: Thermoplasma Volcanium
What Happened to the Dinosaurs?
Bacterial ShapesBacterial Shapes
Modified ShapesModified Shapes
• Diplo• Strepto• Staphylo• Vibrio• Tetrad• Pleomorphic – can assume more than 1 shape• Monomorphic – can assume only one shape
Spirillum
Glycocalyx/Slime Capsule/Slime LayerGlycocalyx/Slime Capsule/Slime Layer
Not Really the Same ThingNot Really the Same Thing
• Slime Capsule is a distinct Defined Layer with a Distinct Outer Edge
• Slime Layer is a Poorly Defined Concentration of Slime. It Lessens with Distance.
• Glycocalyx Refers to a Gelatinous Polysaccharide and/or Polypeptide Covering.
Purposes
1. Protects Pathogens from Phagocytosis
2. Enable Adherence to Surfaces
3. Protects Against Desiccation
4. Stores Food Reserves
Case Study 1Case Study 1• The patient was a 22-year-old female with a history of
mitral valve prolapse (a defect of the valve between the left atrium and ventricle caused by a weakening of the tough, connective tissue of the valve leaflets, which allows the valve to project back into the left atrium). She was admitted with complaints of intermittent fevers for 1 month and headaches for 3 weeks. Two weeks before symptoms developed she had undergone a dental procedure.
• Four blood cultures were performed on admission. All four blood cultures demonstrated Gram-positive cocci in chains.
The diagnosis is bacterial endocarditis (a bacterial infection of the tissue lining the inside of the heart; usually involves the heart valves).
The patient had recently had her 6 month dental appointment to have her teeth cleaned.
• During dental procedures, transient bacteremia occurs in up to 80% of individuals.
• Transient bacteremia is defined as the presence of bacteria in the bloodstream for short periods.
• The organisms that cause this are generally of low virulence and are usually easily removed by the reticuloendothelial [filtering and phagocytic] system.
Case Study 2Case Study 2
• This 47-year-old man had a history of sickle cell disease that resulted in many previous hospitalizations for the management of painful crisis.
• The patient had been admitted 9 days prior to the current admission for management of such a crisis, and a right port-a-cath (a central venous catheter that is designed to remain in place for a prolonged period) was placed in his right subclavian vein.
• He was discharged (with port-a-cath remaining) after a 4-day hospitalization.
• On the day of readmission, the patient had right arm discomfort and swelling, a temperature of 38.1C (normal is 37C), and chills. He presented to the hospital emergency room, where he was afebrile. Physical examination was remarkable for right extremity swelling.
• Two blood cultures were obtained (one set through the port-a-cath and one set via a peripheral vein). The two sets of blood cultures grew identical Gram-positive cocci that were catalase positive. The diagnosis is in-line sepsis.
Any indwelling device introduced through the skin places an individual at risk for infection.
Bacteria can produce a slime layer that can enhance their adherence to a wide variety of plastic surfaces.
Slime-producing strains of staphylococci may also be more difficult to eradicate by antimicrobial therapy than non-slime-producing ones due to antibiotic resistance.
FlagellaFlagella
Hook is similar to a universal joint
Basal Body penetrates the cell and causes the flagellum to rotate. It serves as a “motor”.
Filament is actually an extension of the plasma membrane.
Flagella Occur In a Variety of
Forms
Examples of bacterial flagella
arrangement schemes.
A-Monotrichous (one flagella)
B-Lophotrichous (a tuft of flagella)
C- Bipolar Monotrichous (one flagella at both ends)
D-Peritrichous (a hair ball)
• Monopolar Lophotrichous
• Bipolar Lophotrichous
• Peritrichous
Identify This
Identify This
Identify This
Identify This
Flagella Allow for MotilityFlagella Allow for Motility
Bacterial Bacterial MovementMovement
TaxisTaxis
• Positive Taxis• Negative Taxis• Phototaxis• Chemotaxis• Magnetotaxis
Plankton
AntigenAntigen
• Bacteria Flagella is composed of protein which acts as an antigen when introduced into the human body.
• What happens then?
• Why is this beneficial for vaccines?
Axial FilamentsAxial Filaments
(Endoflagellum)(Endoflagellum)
on a Spirocheteon a Spirochete
SpirochetesSpirochetes• Spirochetes are long and slender bacteria that are
tightly coiled, and so look like miniature springs or telephone cords.
Spirochetes Cause Spirochetes Cause Syphilis and Lyme Syphilis and Lyme
DiseaseDisease
Are Spirochetes and Spirilla the Same?
Fimbriae and PiliFimbriae and Pili
Short, Rigid, Hollow, Thin, Protein Appendages
They are NOT involved in motility.
Fimbriae Help Cells Adhere To Surfaces
Pili Join Cells for the Transfer of DNA From One Cell to Another
Together They Produce Biofilms.
This is Biofilm in a Swamp Gas
Reactor
Biofilm on Teeth
The Cell Wall of PeptidoglycanThe Cell Wall of Peptidoglycan
Gram Positive BacteriaHave Thicker Layer of Peptidoglycan and Have Teichoic Acids
Gram Negative BacteriaThinner Layer of Peptidoglycan
Gram + and – Cell Walls
Gram - BacteriaGram - Bacteria• More susceptible to breakage• Thinner layer of peptidoglycan
Outer membrane is strongly negative aiding is resisting phagocytosis and
providing a barrier to antibiotics and
digestive enzymes
Periplasmic space containing specialized proteins and enzymes involved in nutrient
acquisitionIt has Porins and Specific Channel
Proteins
Osmotic EffectsOsmotic Effects
Water Moves Freely Across Membranes to Try and Equalize Concentration
Osmotic Differences in Blood Cells
Lysozyme and PenicillinLysozyme and Penicillin
More Basic Structures of Prokaryotic Cells
Cell Membrane
Cell Cell MembraneMembrane
• Permeability Barrier or Selective Barrier• Prevents cell contents from leaking away• Impermeable to polar and charged
molecules• Contains specific proteins to carry out
selective transport• 50% lipid and 50% protein
Very delicate and easily ruptured
Phospholipid BilayerPhospholipid Bilayer
50% Lipid
50% Protein
Embedded ProteinsEmbedded ProteinsFluid Mosaic Model of Membrane StructureFluid Mosaic Model of Membrane Structure
Cytoplasm Filled with RibosomesCytoplasm Filled with RibosomesRibosomes Utilize 25% Cell’s Volume – 90% Cell’s
Energy
RibosomesRibosomes
• Composed of RNA and Protein
• Function to Make Protein (Mainly Enzymes)
• Size is Measured in Svedberg (S) units
• Bacterial Ribosomes are ~70S
NucleoidNucleoid
Plasmids are small circular DNA elements found in virtually all bacterial cells.
They carry extra genes and are important in Genetic Engineering.
Inclusion Bodies or Storage Inclusion Bodies or Storage GranulesGranules
• Fats• Sugars• Phosphates• Sulfur• Metachromatic
Granules
Prokaryotic bacteria exist in very competitive environments where nutrients are usually in short supply, so they tend to store up extra nutrients when possible.
EndosporesEndospores
Survival Mechanism for Extended Periods in the Absence of Food, Water, of Proper Growth Conditions
EndosporesEndospores
• Can resist Sterilization• Can resist Boiling• Can resist UV Light• Can resist Dessication• Can resist Harmful Chemicals• Abundant in Bacillus and Clostridia• Germination in Minutes• Create a Problem with Canned Foods
We worry about
canned goods and botulism.
Oval Terminal
Rectangular Terminal
Rectangular Subterminal
Rectangular Central
Circular Terminal
Circular Central
Club Shaped Terminal
BacteriaEndospores
Schaeffer Fulton Method of Staining
Dorner Method of Staining
Dating EndosporesDating Endospores
• Spores taken from prehistoric mosquitoes suspended in Amber
• Roman Fort Vindolanda- 1976 Fort dating AD 90-95 was drained and excavated
- Found viable endospores of Thermoactinomyces vulgaris - Thermophilic Aerobic Bacterium (Warmth and Moisture)- Had been sealed in a cold anaerobic environment (flooded)- Would date this particular endospore to being 2000 years
old
Eukaryotic Cells
CiliaCilia
Chromosomes and ChromatinChromosomes and Chromatin
Nucleus with Double Nucleus with Double MembraneMembrane
MitochondriaMitochondria
Endosymbiotic Theory of Cellular Endosymbiotic Theory of Cellular EvolutionEvolution
• Proposed by Lynn Margulis in 1981
• Studied Mitochondria
• Saw association between Mitochondria and Gram-negative Bacteria
Endomembrane SystemEndomembrane System
• Endoplasmic Reticulum
• Golgi Apparatus• Various Vesicles• Lysosomes• Microbodies• Nuclear Membrane
Rough E.R.
Functions InFunctions In
• Compartmentalization• Chemical Homeostasis• Communication• Transport
Golgi Apparatus
Membrane TransportMembrane Transport | |
Small Molecules Large Molecules
| | | |
Passive Transport Active Transport Endocytosis Exocytosis
| |
Diffusion Phagocytosis
Osmosis Pinocytosis
Facilitated Diffusion
ExocytosisExocytosis
EndocytosisEndocytosis
Phagocytosis in Action
Endosymbiotic Theory / Endosymbiotic Theory / EukaryotesEukaryotes
• The endosymbiotic theory concerns the mitochondria, chloroplasts, and possibly other organelles of eukaryotic cells.
• According to this theory, certain organelles originated as free-living bacteria that were taken inside another cell as endosymbionts.
• Mitochondria developed from proteobacteria such as Rickettsiales, and chloroplasts from cyanobacteria.