Center for Homogeneous DNA Analysis - Software

April 21, 2005

Second Year Advances

• Background removal

• Melting curve calculators

• Automatic clustering and classification

Background removal

• Superior to baseline method• Enables correct classification of unlabeled

probe melting curves• Makes HLA matching, repeat typing and

sequencing more accurate• Improves determination of thermodynamic

parameters, and therefore all PCR design and analysis tools

• In use by ARUP Labs

Melting curve calculators

• More informative than just Tm calculator

• Enhances design of full genotyping studies

• Improves determination of thermodynamic parameters

Automatic clustering and classification algorithms

• Used in HLA identity testing, mutation scanning, and genotyping

WWW availability

• Center for Homogeneous DNA analysis• Homepage: www.dna.utah.edu• DNAWizards beta site by remote panels• URL: DNAWizards.path.utah.edu• Small amplicon primer design: SNPWizard• Melting curve calculators: Melt1.0, Het1.0

The coming year

• Determine a complete set of Tm Parameters • superior background removal• realistic experimental conditions (dyes, salt)• mismatched duplexes (synthetic)

• Genome wide primer promiscuity prevention• Further develop user-friendly suite of programs• Commercial launch of DNAWizards.com