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Chapter 1BOT3015L
Plant Biology Laboratory
Presentation created by Danielle Sherdan and edited by Jean Burns-Moriuchi and William Outlaw
All photos from Raven et al. Biology of Plants except when otherwise noted
Scientifically supported relationships between the major groups of organisms
Tree of life project read more at www.tolweb.org
"The affinities of all the beings of the same class have sometimes been represented by a great tree... As buds give rise by growth to fresh buds, and these if vigorous, branch out and overtop on all sides many a feebler branch, so by generation I believe it has been with the great Tree of Life, which fills with its dead and broken branches the crust of the earth, and covers the surface with its ever branching and beautiful ramifications."
Charles Darwin, 1859
Obscure root of the tree of life
Nucleate cells
Plants Animals
Insects and Arthropods
Vertebrates
Fungi
Prokaryotes (no photo)
Notice also that there are branches that connect to other branches to represent endosymbiosis
Today
• Day-by-day syllabus review• Class policy• Science and maintaining a lab notebook• Plants are our sustenance• Crop investigations
• Day-by-day syllabus review• Class policy• Science and maintaining a lab notebook• Plants are our sustenance• Crop investigations
Chapter 2Characteristics of plant cells
and plant-cell division
From Collin County Community College District BioLab
Elodea leaf
• Cellulosic cell walls
• Plastids such as chloroplasts for oxygenic photosynthesis
• Large vacuoles
Chapter 3Flowering plants
From Outlaw lecture
Evolution and function of floral parts and pollination
Chapter 5Effects of plant
hormones on plant growth
Control, a dwarf plant
A dwarf plant treated with gibberellin, a plant hormone
Chapter 6Germination
Primary Growth
Maize seed consists of seed coat containing the
embryo and nutritive tissue
Germinating maize seed
Chapter 7Experimenting with Guard cells
Guard cells and stomata in the epidermis of Vicia faba
Stomata open Stomata closed
Photos from Outlaw’s lab and also featured on the cover of the scientific journal Archives of Biochemistry and Biophysics in 2002
Chapter 11 Fungi
Lichens are symbiotic relationships between fungi and green algae and/or cyanobacteria
Morel, one of many edible fungi
Chapter 12Non-flowering plants
gymnospermsbryophytes ferns
Marchantia sp.liverwort Matteuccia sp. Pinus sp.
Today
• Day-by-day syllabus review• Class policy• Science and maintaining a lab notebook• Plants are our sustenance• Crop investigations
Class Policy
Prerequisites
Insurance
Required materials
Attendance
ADA Statement
Academic Honor Policy
Performance and Participation
Safety
Grading
Lab notebookPencilTextbookLab manual
1. Plagiarism2. Cheating3. Unauthorized group
work4. Fabrication5. Falsification6. Misrepresentation7. Resubmission
Prior to each class:Read the lab materialsDrawing listReview questionsProtocols
Attendance and statement of understanding class policy
Today
• Day-by-day syllabus review• Class policy• Science and maintaining a lab notebook• Plants are our sustenance• Crop investigations
Scientific Skills
Keen observations are recorded
The specimen
What are your observations?
How would you best record observations about this specimen?
From Collin County Community College District BioLab
Example drawing
Example drawing of a similar specimen
How does the drawing represent the specimen well?
How could the drawing better represent the specimen?
Record your observations accurately and thoroughly
From Collin County Community College District BioLab
Student work, BOT3015
Record interpretations with drawings that emphasize important aspects
Observing specimens
If your specimen has several cell types, draw a few
cells of each type exactly as they appear and
emphasize important information (e.g. cell-type
distribution, patterns, cell variation, sections) with
diagrammatic sketches and descriptions.
Notebook (left / right)
Left(thinking)
Right(lab work)
• Observations• Solution preparation• Methods• Data collected
during experiment
•Interpretations•Conclusions•Graphs•Ideas for future experiments
•Answers to review questions and objectives
Leave space in front for table of contents
******************************13 June 2005
Treatment of Brassica rapa plants with GA
Time: 10:05 am
_√__ Measure plant heights
1. _20_ mm2. _40_ mm
_√_ Apply 20 µl 100 µM GA to 1st leaf of plants 1, 2 and 3.
___ Apply 20 µl 10 µM GA to 1st leaf of plants 4, 5 and 6.
____ ……..etc.…….
Today
• Day-by-day syllabus review• Class policy• Science and maintaining a lab notebook• Plants are our sustenance
– Effects of agriculture on animals– Effects of agriculture on plants
• Crop investigations
Agriculture, the primary means of procuring food since ~10,000 years
ago (less than 1% of human existence)
MaizeCalled “Indian corn” to distinguish it from other Old
World Grains, but in American English, the name has been shortened to simply “corn.”
From Outlaw’s garden, BOT3015 lecture
Today
• Day-by-day syllabus review• Class policy• Science and maintaining a lab notebook• Plants are our sustenance• Crop investigations
– Choose a crop– Research via text and computer– Presentations