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CHAPTER 13
GENE REGULATION
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Mutation
Mutation is a permanent change in the sequence of bases in DNA. Protein is completely inactivated
Germ-line mutations occur in sex cells Somatic mutations occur in body cells
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Causes of Mutations
Spontaneous mutation DNA can undergo a chemical change Replication Errors
1 in 1,000,000,000 replications DNA polymerase
Proofreads new strands Generally corrects errors
Induced mutation: Mutagens such as radiation, organic chemicals Many mutagens are also carcinogens (cancer causing) Environmental Mutagens
Ultraviolet Radiation Tobacco Smoke
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Causes of Mutations
Ultraviolet (UV) radiation is easily absorbed by the pyrimidines in DNA.
Cause neighboring thymine molecules next to one another to bond togetherThymine dimers.
C G
C
A
A
G
kink
thyminedimer
Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display.
T
T
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Causes of Mutations
Usually, these dimers are removed by DNA repair enzymesDeficient DNA repair enzymes leave the skin
cells vulnerable to the mutagenic effects of ultraviolet light
Accumulation of mutationHigh incidence of cancer
CHROMOSOME MUTATIONS
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AN EXAMPLE: Translocation has been pinpointed as the cause of chronic myeloid leukemia, a type of cancer (of the white blood cells)
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GENE MUTATIONS
Point Mutations (Substitutions)MissenseNonsenseSilent
Frameshift Mutations InsertionDeletion
2727
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GENE MUTATIONS: Effect of Mutations on Protein Activity
Point Mutations Involve change in a single DNA nucleotide Changes one codon to a different codon Affects on protein vary:
Nonfunctional Reduced functionality Unaffected
Frameshift Mutations One or two nucleotides are either inserted or deleted
from DNA Protein always rendered nonfunctional
Normal : THE CAT ATE THE RAT After deletion: THE ATA TET HER AT After insertion: THE CCA TAT ETH ERA T
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
The molecular basis of sickle-cell disease: a point mutation
In the DNA, themutant templatestrand has an A where the wild-type template has a T.
The mutant mRNA has a U instead of an A in one codon.
The mutant (sickle-cell) hemoglobin has a valine (Val) instead of a glutamic acid (Glu).
Mutant hemoglobin DNAWild-type hemoglobin DNA
mRNA mRNA
Normal hemoglobin Sickle-cell hemoglobin
Glu Val
C T T C A T
G A A G U A
3′ 5′ 3′ 5′
5′ 3′5′ 3′
Sickle Cell
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Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Base-pair substitution
Wild type
A U G A A G U U U G G C U A AmRNA 5′Protein Met Lys Phe Gly Stop
Carboxyl endAmino end
3′
A U G A A G U U U G G U U A A
Met Lys Phe Gly
Base-pair substitutionNo effect on amino acid sequence
U instead of C
Stop
A U G A A G U U U A G U U A A
Met Lys Phe Ser Stop
A U G U A G U U U G G C U A A
Met Stop
Missense A instead of G
NonsenseU instead of A
Copyright © 2005 Pearson Education, Inc. publishing as Benjamin Cummings
Base-pair insertion or deletion
mRNA
Protein
Wild type
A U G A A G U U U G G C U A A5′
Met Lys Phe Gly
Amino end Carboxyl end
Stop
Base-pair insertion or deletionFrameshift causing immediate nonsense
A U G U A A G U U U G G C U A
A U G A A G U U G G C U A A
A U G U U U G G C U A A
Met Stop
U
Met Lys Leu Ala
Met Phe GlyStop
MissingA A G
Missing
Extra U
Frameshift causing extensive missense
Insertion or deletion of 3 nucleotides:no frameshift but extra or missing amino acid
3′
Tay-Sachs DiseaseFrameshift Mutation:
• Caused by 4 bp INSERTION in exon 11 of the HEXA gene on chromosome 15
• This causes a stop codon in the wrong location
• Deficient hexosaminidase (hex A).
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Crohn’s DiseaseFrameshift Mutation
Inflammation of the digestive tract….usually small intestine affected the most
Originally thought to have been caused by environmental factors
In 2001 it was determined that an INSERTION of cytosine into the NOD2 gene of DNA
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Cystic FibrosisFrameshift Mutation
Caused by a DELETION of three base pairs on the CFTR gene.
phenylalanine not located at position 508 in the protein
mutation is often called F508
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Carcinogenesis
Development of cancer involves a series of mutations
Proto-oncogenes – Stimulate cell cycle
Tumor suppressor genes – inhibit cell cycle
Mutation in oncogene and tumor suppressor gene:
Stimulates cell cycle uncontrollably
Leads to tumor formation