Chapter Nine

Facility Equipment

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Facility Equipment

Specialized equipment = cage racks, floor scrubbers, autoclaves, vacuum cleaners and cage washers a large capital expense in the operating budget

It is important that animal technicians learn the appropriate care and use for each piece of equipment.

When equipment fails to operate properly, it must be immediately taken out of use. The failure can then be reported to the appropriate

manager and the equipment repaired or replaced as quickly as possible.

Caging

Depends on nature of research & husbandry

Safe cages have smooth surfaces with no jagged edges, broken wire rungs or rust.

Escape-proof & well ventilated to maintain environment

Poorly ventilated cages - water and urine may soak animals and increase humidity.

Dirt and waste deposits collect cracks & crevices.

Proper cage design permits safe handling of animals & protects animal handlers.

Caging Materials

Stainless steel smooth, durable, no rust &

is impervious to descalers

expensive, but durability offsets high price

Aluminumweighs less than stainless steel but is less durable

Galvanized steel, iron, & woodnot suitable caging materials

rust & don’t stand up to most chemical cleaners

wood - rough, splintery surface, difficult to sanitizesealed w/ epoxy paint is more easily sanitized, but does not

stand up to high wash temps or scrubbing

Plastic Caging Materials

Polystyrene melts or warps at wash temp, moderate strengthused for single use disposable rodent caging

Polypropyleneholds up at high wash temperatures but is

opaque non-transparent may be preferable for solitary

species Polycarbonate

high impact strength, transparent, holds up at high temps

Plastics do not rust, their surfaces are smooth, & they are impervious to chemical cleaners

Shoebox Rodent Cages

Solid-bottomed plastic or stainless steel cage

Contact bedding is generally used.

The water bottle and feeder are easily visible.

Microisolation systems > control of environment

Micro-Isolator™ has own filter top to limit air

exchange between room & cage interior.

May sit on standard rack, or ventilated rack.

Procedures inside hooded, ventilated workbench. feeding, watering, cage changing & manipulations

(Images) Microisolator Changing Hood

Micro-Isolator™ Courtesy of Lab Products, Inc.

Suspended Cage Systems

Have perforated or solid bottom

Wastes drop through bottom into collection pan.

routinely emptied and cleaned

Flooring can injure or irritate animals’ feet & legs.

Plastic-coated mesh and bars reduce irritation & provide a warmer, more comfortable surface.

Smooth metal floors are comfortable alternative.

May have individual lids, or suspended on runners which enable shelves to serve as lids.

Wire mesh cages better ventilation than solid.

Newborns fall through wire; direct contact w/ metal draws heat away from animals’ bodies.

Front-Opening & Metabolism Cages

Front-Opening CagesAvailable as individual or multiple-cage rack Bar or wire mesh flooring w/ collection traysFeeders & bottle holders attach to cage door.Rabbits, cats, dogs & primates usually housed in front-

opening cages.

Metabolism cages designed to separate urine & feces for specimen collection. frequently & carefully hand cleanedprevent feed & water from mixing with specimenspowdered feed helps prevent dropping into collectiondrinking valve outside cage to avoid contamination of

collected urine sample

Two Types of Mouse Metabolism Cages

Gang Cages

House groups of same animal species Resting boards or perches make better use of

space. Bedding on the cage floor depends on species. Feed and water offered in >1 location to avoid food

hoarding by dominant animals. Cats, primates & sheep Introducing/creating

group may result in

fighting.Make careful

observation of group.

Transport Cages Shipping cartons - temporary transport housing Moist mash, fresh fruits or vegetables can be source of moisture. AWA regulates shipment of certain species, such

as guinea pigs, hamsters, rabbits, cats, dogs & nonhuman primates.Regulations cover shipping carton construction, space

requirements, documentation, feed & water schedules. Transport cages within a facility

Feed, water & bedding usually not required, since animals only in cage for a short time.

If transporting in public areas, advisable to use covering.

Runs or Pens

Runs or pens are used for larger animals.

Large enclosures usually with waterproof flooring & often with a resting area elevated above floor

Floor slopes to aid drainage of water and urine away from housing & feeding areas.

Surfaces should be easy to clean and sanitize.

When built outdoors, pens & runs must also include shelter from inclement weather.

Pens and runs are commonly used to house dogs, sheep, pigs and goats.

Feeding & Watering Accessories Pelleted feed held in slotted V-shaped feeders in

cage lids or in feeders clipped onto cages. J-shaped feeders used for smaller sized pelleted

rabbit and guinea pig feed. Feed & water bowls on floor used for dogs & cats. Suspended or detachable feeders that keep feed

off the floor help eliminate contamination. Water - glass or plastic bottles, stainless steel or

rubber bowls, pails or automatic watering system Bottles or bowls to monitor amount consumed

Some types of medications administered in water.

(Image) Watering and Feeding Devices

Automatic Watering

Eliminates work associated with water bottles.

Water through piping to animal area, where pressure is reduced to suitable levels.

Pressure reducing station has 1 - 2 pressure regulators

filters out particulates from the water supply

Water then passes into room distribution system to direct water to cage rackRetractable recoil hoses tie room system with animal

cage rack manifold system.Designed to pipe water to each cage and provide a direct source

of water via a drinking valve device.

(Images) Automatic Water FlowAutomatic Water Flow

1

Treatment Tanks

2 Pressure Reduction

Station

3 Recoil Hose

4

Sipper

Automatic Watering II

Individual valves must be checked regularly. Clogged or leaking valves should be replaced

immediately or identified as nonfunctional. Check pressure station gauges daily. Flush to reduce accumulation of microorganisms. Some facilities use chemicals such as chlorine to

sanitize water systems. It may be necessary to provide newly received

animals with water bottles until they learn to use the automatic watering system.Rabbits often require several days to adjust.An animal that is thirsty will not eat.

Cage Wash Equipment

Cabinet washers - loaded & sanitized in chamberWasher goes through washing & rinsing cycles followed

by a drying cycle.

Rack washers are large versions of cabinet washers - racks of caging directly rolled in.single door or pass-through

Tunnel cage washer is like a commercial car wash.carries equipment through washer on a conveyor beltseries of stations wash, rinse & dry

Cage wash controls permit temp control, timing of cycles, detergent addition & emergency shutoff.also have monitoring and/or recording gauges

(Images) Scenes From Cage Wash

Cage Wash Equipment II

Cleaning filters & spray valves

ensure that spray valves do

not become plugged. Experienced specialists examine & service

motors, gaskets & electrical components. Check timers & gauges daily to ensure

equipment is properly sanitized. Some facilities use pressurized steam cleaners to

complement initial hand cleaning of cages.When no cage washers are available, these cleaners

may be primary method for cage sanitation in conjunction with disinfectants and detergents.

(Images) Cage Wash Equipment

Balances

Technicians may weigh animals, obtain body temperatures or take other measurements.

Capacity of a scale or balance refers to max. weight device can measure accurately.

Accuracy of a balance refers to degree measured weight = actual weight.

An efficient balance allows small weight increments to be distinguished with accuracy.

Speed and ease of operation important in choosing the balance best suited for a particular job.

Top-loading balances are most common.

Balances II

Triple-beam & double-pan for smaller species. Large species platform scales read weight

directly, similar to a home bathroom scale.Some are designed so animal is restrained.

Top-loading balances w/ digital readouts.Pan weight set at zero automatically to eliminate

weight of restrainers before measuring animal.Some models provide printout.

Electronic balances can input data to a computer. Cleaning scales after each use is important to

prevent transmission of disease & prevent dirt & hair accumulation from affecting scale operation.

Additional Reading

Poole, Trevor (ed.). The UFAW Handbook on the Care and Management of Laboratory Animals, 6th. Ed. Longman Scientific and Technical, Essex, England. 1987

Rollin, Bernard E., and M. Lynne Kesel (ed.). The Experimental Animal in Biomedical Research. Volume II: Care, Husbandry and Well-Being — An Overview By Species. CRC Press, Boca Raton, FL. 1992