Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris

cv. Blauhilde

Brandon Lew

March 24, 2007

Lab Work - Hamline University

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Goal: To identify pigments that cause the purple coloration of pod coats in Phaseolus vulgaris cv. Blauhilde

Hypothesis: The purple color is caused by anthocyanins

Phaseolus vulgaris

• Kidney, black, navy, and snap beans

• Anthocyanins found in:– Seed coat of kidney beans

Choung et al. 2003

– Seed coat of black beansTakeoka et al. 2005

• Neither pod coats, not cultivar Blauhilde studied

Cultivar Blauhilde

• Pod coats purple• Purple disappears when cooked

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Anthocyanins

• Pigments

• Anti-carcinogenicKatsube et al. 2003

• Anti-oxidativeTamura and Yamagami, 1994

Colorful,Good for health

Methods

High-Performance Liquid Chromatography (HPLC)

Small substances

Large substances

HPLC of Extract

Isoabsorbance of Extract

HPLC after Purification

Isoabsorbance after Purification

Mass Spectroscopy

Detector

FragmentsMoleculeMagnets

e-

Mass Spectrum of Extract

Identified Molecule

OOH

OHOH

OH O

OH

OH

OHOH

O+

OH

O

O

OCH3

OH

OCH3

Malvidin 3,5-diglucoside

Conclusion

• Identified anthocyanin as malvidin 3,5-diglucoside

• 86% of total anthocyanin content

Future Work

• Genetically engineer different anthocyanins– Keeps health benefits– Different colors

Acknowledgements

• Dr. John Matachek• Hamline University Chemistry Dept.• Ms. Fruen• Ms. Richter• Team Research

Characterization of Anthocyanins in Purple Pods of Phaseolus vulgaris

cv. Blauhilde

Brandon Lew

March 24, 2007