1. Introduction to Laboratory Safety1.1Preparing for laboratory work1.2During laboratory work1.3Cleaning up before leaving1.4Evaluating laboratory hazards, an ongoing process1.5Working alone policy

2. Workplace Hazardous Materials Information System (WHMIS)2.1Regulatory Requirements: Labelling, Material Safety Data Sheets & Training2.1.1Labelling2.1.1.1Suppliers Labels2.1.1.2Workplace Labels2.1.1.3Workplace Labels in Research Laboratories2.1.1.4EHS Approved Lab Abbreviations List2.1.1.5Laboratory Sample Labels2.1.2Material Safety Data Sheets (MSDS)2.1.2.1Suppliers Responsibilities2.1.2.2Laboratorys Responsibilities2.1.2.3MSDS location2.1.2.4MSDS location indicated on Laboratory Information Card2.1.2.5MSDS Audit2.1.3Training2.1.3.1Core WHMIS Training2.1.3.2Job-specific WHMIS Training2.2Understanding hazard warning information2.2.1WHMIS Symbols2.2.2Toxicological properties: LD50AND LC502.2.3Exposure limits (TLV, PEL)2.2.4Flash point2.2.5Autoignition temperature2.2.6Flammable limits

3. Control of Chemical Hazards3.1Toxic chemicals and the four routes of entry3.2Flammable chemicals3.3Oxidizing chemicals3.4Reactive chemicals3.5Corrosive chemicals3.6Chemical spill response3.6.1Spill response contingencies3.6.2Development of spill response plans3.6.2.1Communications3.6.2.2General guidelines3.6.3Guidelines for specific types of spills3.6.3.1Flammable and toxic liquids3.6.3.2Corrosive liquids3.6.3.3Corrosive solids3.6.3.4Toxic solids3.6.3.5Gases3.6.3.6Mercury3.6.3.7Special categories

4. Storage and Handling in Laboratories4.1General Storage Guidelines4.2Ergonomics4.3Chemical Storage4.4Flammable Liquid Storage Cabinets4.5Chemical Compatibility4.6Chemical Segregation4.7Unstable Chemicals4.8Explosive Chemicals

5. Fire Safety5.1The fire triangle5.2Classes of fire5.3Fire extinguishers5.4Preventing fires5.5Evacuations

6. Hazardous Waste Disposal6.1Waste minimization6.2Hazardous waste disposal guidelines6.3Waste preparation procedures6.3.1Chemical waste6.3.1.1Organic solvents and oils6.3.1.2Miscellaneous chemicals and cylinders6.3.1.3Chemicals of unknown composition6.3.1.4Peroxide-forming (e.g. ether) and explosive (e.g. dry picric acid) chemicals6.3.1.5Corrosives (acids and bases)6.3.2Biomedical waste6.3.2.1Animal carcasses6.3.2.2Infectious laboratory waste6.3.2.3Biohazardous sharps6.3.2.4Blood and blood-contaminated materials6.3.3Sharps6.3.3.1Definition of Sharps6.3.3.1.1Contaminated sharps6.3.3.1.2Non-contaminated sharps6.3.3.2Broken glassware (uncontaminated)6.3.3.3Empty chemical reagent bottles6.3.4Radioactive waste6.3.4.1Solid waste (except sealed sources)6.3.4.2Sealed and encapsulated sources6.3.4.3Liquid scintillation vials6.3.4.4Liquid radioactive waste

7. Laboratory Ventilation and Fume Hoods7.1General ventilation7.2Local ventilation devices7.2.1Chemical fume hoods7.2.2Canopy hoods7.2.3Slotted hoods7.2.4Biological safety cabinets7.2.5Direct connections7.3Ventilation balancing and containment7.4Safe use of chemical fume hoods

8. Compressed Gases and Cryogenics8.1Hazards of compressed gases8.2Safe handling, storage and transport of compressed gas cylinders8.3Cryogenic hazards8.4Cryogenic handling precautions

9. Physical Hazards and Ergonomics9.1Electrical safety9.2High pressure and vacuum work9.3Repetitive work and ergonomics9.4Glassware safety

10. Equipment Safety10.1Centrifuges10.2Electrophoresis equipment10.3Heating baths, water baths10.4Shakers, blenders and sonicators10.5Ovens and hot plates10.6Analytical equipment10.6.1Scintillation counters10.6.2Atomic absorption (AA) spectrometers10.6.3Mass spectrometers (MS)10.6.4Gas chromatographs (GC)10.6.5NMR equipment10.6.6HPLC equipment10.6.7LC/MS equipment

11. Personal Protective Equipment11.1Eye and face protection11.2Lab Coats11.3Hand Protection11.3.1Latex gloves and skin reactions11.3.2Glove selection guidelines11.3.3Chemical glove selection11.3.4Selection, use and care of protective gloves11.4Respirators11.4.1Selection, use and care of respirators

12. Emergency Procedures12.1First aid12.1.1Burns12.1.1.1Burns to the skin12.1.1.2Burns to the eyes12.1.2Cuts12.1.3Needlestick injuries12.1.4Chemical splashes to the skin or eyes12.1.5Poisoning12.2Fires12.2.1Suspected fires12.2.2Known fires12.2.3Clothing fires12.3Hazardous chemical spills12.4Natural gas leaks

Appendix 1: Flammability classification & permissible container sizes1. Introduction to Laboratory Safety1.1 Preparing for laboratory workBefore starting to work in a laboratory, familiarize yourself with the following: the hazards of the materials in the lab, as well as appropriate safe handling, storage and emergency protocols. Read labels and material safety data sheets (MSDSs) before moving, handling or opening chemicals. Never use a product from an unlabeled container, and report missing labels to your supervisor. the agents, processes and equipment in the laboratory. If you are unsure of any aspect of a procedure, check with your supervisor before proceeding. the location and operation of safety and emergency equipment such as fire extinguishers, eye wash and shower, first aid and spill response kits, fire alarm pull stations, telephone and emergency exits emergency spill response procedures for the materials you will handle emergency reporting procedures and telephone numbers designated and alternate escape routes1.2 During laboratory work Restrict laboratory access to authorized persons only. Children are not permitted in labs. Smoking; eating; drinking; storing food, beverages or tobacco; applying cosmetics or lip balm and handling contact lenses are not permitted in laboratories. Wear lab coats (knee length) and safety glasses in laboratories employing chemicals, biohazards or radioisotopes. Open shoes, such as sandals, should never be worn in the lab. Tie back or otherwise restrain long hair when working with chemicals, biohazards, radioisotopes, or moving machinery. Keep work places clean and free of unwanted chemicals, biological specimens, radios, and idle equipment. Avoid leaving reagent bottles, empty or full, on the floor. Work only with materials once you know their flammability, reactivity, toxicity, safe handling and storage and emergency procedures. Consult material safety data sheets (MSDS) before working with hazardous chemicals or infectious material. Replace MSDS that are more than 3 years old. Prepare and maintain a chemical inventory for the lab. Never pipette by mouth; use mechanical transfer devices. Walk, do not run, in the lab. Keep exits and passageways clear at all times. Ensure that access to emergency equipment (eyewashes, safety showers and fire extinguishers) is not blocked. Report accidents and dangerous incidents ("near-misses") promptly to your supervisor Wash your hands thoroughly before leaving the laboratory. Conduct procedures involving the release of volatile toxic or flammable materials in a chemical fume hood (See Section7.4). Perform procedures that liberate infectious bioaerosols in a biological safety cabinet Handle all human blood and body fluids as if potentially infectious1.3 Cleaning up before leavingPerform a safety check at the end of each experiment and before leaving the lab. Make sure to: Turn off gas, water, electricity, vacuum and compression lines and heating apparatus Return unused materials, equipment and apparatus to their proper storage locations Label, package and dispose of all waste material properly (Refer to Section9.3, "Waste Preparation Procedures") Remove defective or damaged equipment immediately, and arrange to have it repaired or replaced Decontaminate any equipment or work areas that may have been in contact with hazardous materials. Leave behind protective clothing (lab coats, gloves, etc.) when leaving the laboratory Close and lock the door to the laboratory if you are the last one to leave1.4 Evaluating laboratory hazards, an ongoing processThere are many categories of hazards that might be encountered in a laboratory setting, and situations can change frequently. Even after you have identified and controlled all current risks, it is critical that you remain open to the possibility that new unexpected dangers can arise. Periodically verify that the Laboratory Information Card (LIC) and other hazard warnings are current; advise Environmental Health and Safety whenever changes to the LIC are required.Carry out weekly inspections on the condition of: fire extinguishers emergency wash devices such as eyewashes and drench hoses (run these for several minutes and update inspection tags first aid kit contents fume hood and other ventilation devices tubing for circulating water, vacuum, gases chemical storage compartmentsAlso, ensure that fire extinguishers and emergency showers are inspected, tested and tagged annually.Among potential laboratory hazards, be alert for the following: Chemical products flammable toxic oxidizing reactive corrosive Microbiological disease-producing agents and their toxins viruses bacteria parasites rickettsiae fungi Physical or mechanical hazards ionizing and non-ionizing radiation electrical poor equipment design or work organization (ergonomic hazards) tripping hazards excessive noise or heat Psychosocial conditions that can cause psychological stress1.5 Working alone policyWorking alone is an unsafe practice at any time. However, if the nature of your work makes it unavoidable, take measures to ensure that others are aware of your location and have someone check in with you from time to time, either in person or by telephone.Before conducting any work alone in a laboratory go through this checklist to determine if it is appropriate to proceed: Is your supervisor aware of your plans? Are there any hazardous experiments involved?Examples: High temperature High vacuum Extremely flammable materials (low flash point) Poisonous materials Scaling up i.e., higher quantities Have you reviewed your procedure with your supervisor? Do you have a written operating procedure? Are your apparatus and equipment in good working condition? Are you trained to carry out the work? Do you have a check-in/check-out procedure? Do you have an emergency contingency? Do you have access to a McGill telephone (rather than a cell) in case of an emergency? Does your door have a viewing window or other means of indicating someone is inside? Are you aware of the emergency evacuations procedure? Do you have access to a telephone in case of an emergency? Do you have access to a first aid kit? Do you have access to a spill kit?TOP OF PAGE2. Workplace Hazardous Materials Information System (WHMIS)Workplace Hazardous Materials Information System (WHMIS) is a Canada-wide system for providing information on the safe use of hazardous materials, referred to ascontrolled products, in the workplace. It is intended to protect the health and safety of workers by promoting access to information on hazardous materials; this information is provided by means of product labels, material safety data sheets (MSDS) and education programs.WHMIS is governed by federal and provincial laws and regulations (QuebecsRegulation respecting Information on controlled products (R.Q. c. S-2.1, r.10.1) and any person supplying or using controlled products must comply with its requirements. At McGill, WHMIS legislation applies to all faculty, staff, post docs, students (graduate and undergraduate) and visitors who work in areas where hazardous materials are used.Controlled productsare products, materials, and substances that are regulated by WHMIS legislation, based on their hazardous properties and characteristics. WHMIS divides hazardous materials into six main categories or classes based on their characteristics.See Section 2.2The main objectives of WHMIS are hazard identification and product classification. WHMIS consists of three main components: Labelling Material Safety Data Sheets (MSDS) Training2.1 Regulatory Requirements: Labelling, Material Safety Data Sheets & Training2.1.1 LabellingLabels alert people to the dangers of the product and basic safety precautions. It is imperative that all containers in laboratories are clearly identified.WHMIS legislation dictates what information is required on a workplace label. Any hazardous material, whether in transit, storage, or use, must be labelled. A label may be a mark, sign, stamp, device, sticker, ticket, tag, or wrapper and must be attached to, imprinted, stencilled, or embossed on the container of the controlled product.There are 2 types of labels prescribed under WHMIS regulation: supplier labels and workplace labels.2.1.1.1 Suppliers LabelsSuppliers are responsible for labelling WHMIS-controlled products. A supplier label must contain the following information: product identifier (name of product) supplier identifier (name of company that sold it) hazard symbols (WHMIS classification symbols) risk phrases (words that describe the main hazards of the product) precautionary statements (how to work with the product safely) first aid measures (what to do in an emergency) reference to the MSDSSupplier labels must be provided in both official languages (English and French).2.1.1.2 Workplace LabelsA workplace label must appear on all WHMIS-controlled products when: controlled products are produced, manufactured or prepared (e.g., stock solutions) at the workplace; the controlled product is transferred from the original container into another container; and the original supplier label becomes illegible or damaged or when it is removed;A workplace label must contain the following information: product identifier (product name) information for the safe handling of the product reference to the MSDSThe product name must include the full name of the product or solution, as it appears on the material safety data sheet and include its concentration.2.1.1.3 Workplace Labels in Research LaboratoriesWHMIS legislation permits certain exemptions in the labelling requirements for WHMIS-controlled products in laboratories involved with research and development. The following exemptions apply to WHMIS-controlled product manufactured, transferred, used or analyzed in research laboratories, as long as the following conditions are met: the product is not transported outside the laboratory; and the Material Safety Data Sheet is available.In research laboratories, when a WHMIS-controlled product is manufactured, prepared or transferred from one container to another (e.g., stock solutions), the workplace label affixed to the container must contain the following information: product identifier (product name)The product name can either be: the full name of the product or solution, as it appears on the material safety data sheet and including its concentrationOR the approved product abbreviation, as it appears on theEHS Approved Lab Abbreviations ListAbbreviations are not permitted, unless they appear on the EHS Approved Lab Abbreviations List.See Section 2.1.1.4When a non-controlled product is manufactured, prepared or transferred from one container to another, the label affixed to the container must indicate: the product name (abbreviations and chemical formulas permitted)2.1.1.4 EHS Approved Lab Abbreviations ListEHS has compiled an approved list of laboratory abbreviations. This list permits laboratories to use abbreviations on the labels of those products listed.EHS Approved Lab Abreviations List[.pdf]In order to use these abbreviations, the following conditions must be met: The EHS Approved Lab Abbreviations List must be posted in the laboratory, preferably in a location close to where the products are stored;AND The EHS Approved Lab Abbreviations List must be included with the MSDS Collection (e.g., at the beginning of the binder)These conditions will be verified during Laboratory Safety Inspections.The list will be reviewed annually by EHS. If you wish to make suggestions or recommendations for new abbreviations,ehs@mcgill.caEHS (Subject: Lab Abbreviations) and include the full name of the product, the CAS number and attach an electronic copy of the products MSDS.2.1.1.5 Laboratory Sample LabelsLaboratory samples are samples intended solely to be tested in a laboratory or used for educational or demonstration purposes. Laboratory samples do not include WHMIS-controlled products that are used by the laboratory for testing other products, materials or substances (e.g., buffer solutions).The requirements for laboratory samples that are intended to be used in a laboratory immediately (same day) and solely by that person who prepared them include: the samples must be clearly identified; a description of samples contents must be readily available (e.g., noted in a lab book); and Material Safety Data Sheets for the sample must be readily available.Laboratory samples that must be transported outside of a laboratory (e.g., sent elsewhere for analysis), including within the University must have a label affixed to it that contains the following information: product identifier (product name) owners name (name of Principal Investigator who prepared the sample) lab number and building emergency telephone numberWhen samples are greater than 10 kg, the label affixed to the container must meet the requirements of a supplier label (see Section 2.1.1.). Laboratory samples CANNOT be sent via internal mail.2.1.2 Material Safety Data Sheets (MSDS)Material Safety Data Sheets (MSDS) provide more details than labels. They are technical bulletins that provide chemical, physical, and toxicological information about each controlled product, as well as information on precautionary and emergency procedures. They must be readily accessible to anyone who works with, or who may otherwise be exposed to, controlled products.2.1.2.1 Suppliers ResponsibilitiesSuppliers of WHMIS-controlled products are required to make available MSDS to the purchaser. The MSDS must be available in both official languages (French and English). Should any new information arise about a product, the Supplier is required to revise the MSDS.2.1.2.2 Laboratorys ResponsibilitiesEveryone has the right to review an MSDS, whether it is related to their work, or simply because of personal interest.Every lab at McGill must to comply with Quebecs Regulation respecting information on controlled products (R.R.Q. 1981, S-2.1, r. 10.1) which states:"the Material Safety Data Sheet for a controlled product shall be kept at the workplace by the employer in a place that is known to the workers and shall be easily and rapidly accessible to those workers that are liable to come into contact with the product. That data sheet shall be in the form of a document that is easy to handle and consult."-O.C. 445-89, s. 48.The following applies to all laboratories involved with research and development, regardless of the number of controlled products on-hand.Each laboratory is responsible for ensuring that their MSDS Collection: contains the MSDS for all WHMIS-controlled products in the laboratory; contains the MSDS for all consumer products (e.g., Bleach, Windex) in the laboratory; that the MSDS are less than 3 years old; that the MSDS are updated when new information becomes available; and that MSDS are readily accessible to anyone who works with, or who may be exposed to the product.In order to simplify MSDS management, Principal Investigators and Laboratory Supervisors with multiple laboratories can have a central MSDS collection, provided that the labs are reasonably close to each another (in the same building). All lab personnel, including students, must have access 24/7 to the area where the MSDS Collection is kept; if the room is sometimes locked, all personnel must have a key. Everyone must be advised as to the location of the MSDS collection.2.1.2.3 MSDS locationWHMIS legislation requires that a MSDS be readily accessible to anyone who works with, or who may be exposed to controlled products.MSDS Collections may be stored in several ways: a filing cabinet, binders, on a personal computer, or by any other means of storage, provided that all the employees are aware of the location, and are able to gain access to the date sheets at any time.All laboratory personnel must be advised as to the location of the MSDS Collection.EHS recommends all MSDS be placed in alphabetical order in clearly marked red binders in an easily accessible location, preferably close to the telephone.2.1.2.4 MSDS location indicated on Laboratory Information CardThe Laboratory Information Card must contain a detailed description of the location of the MSDS Collection in the laboratory (e.g., second shelf on the black bookshelf).2.1.2.5 MSDS AuditWHMIS legislation requires that a MSDS be less than 3 years old.During Laboratory Safety Inspections, the EHS inspector will audit the MSDS collection. The inspector randomly selects five WHMIS-controlled products found in the laboratory and then verifies the MSDS collection to ensure that it contains the MSDS of the five selected products.2.1.3 TrainingTraining and education provides more detailed instruction on the specific procedures necessary to carry out work safely. WHMIS training is a major component of the WHMIS legislation and therefore is mandatory for all personnel working with controlled products at McGill, including Principal Investigators, students and visiting researcher.Training can broken divided into two parts: Core Training and Job-specific Training.2.1.3.1 Core WHMIS TrainingCore WHMIS Training is basic training that provides instruction on classification of controlled products; include risks and precautions, and the content, purpose and interpretation of information found on labels and in MSDS.Core WHMIS Training for Laboratory Personnel is provided by Environmental Health & Safety and is mandatory for all faculty, staff and students, including undergraduate students working on research projects. The training is valid for a period of 3 years. Core WHMIS Training is offered several times per semester and the schedule can be consulted atwww.mcgill.ca/ehs/training/whmis/.2.1.3.2 Job-specific WHMIS TrainingJob-specific training refers to instruction in the procedures for the safe handling and storage of the WHMIS-controlled products that are unique to each laboratory, and includes spill or leak remediation; waste disposal; and basic first aid instructions. Job-specific training is the responsibility of Principal Investigators and Laboratory Supervisors.Environmental Health & Safety tracks all safety training on campus and is able to supply supervisors with up-to-date safety training lists for all their personnel, including students. To request a safety training attendance list, send an e-mail toEHS.2.2 Understanding hazard warning information2.2.1 WHMIS SymbolsThe classes of controlled chemical products and their corresponding symbols or pictograms, as well as general characteristics and handling precautions are outlined in table 1.Table 1 -Safe handling of controlled products. Summary of general characteristics and procedures for handling and storage of WHMIS-controlled products.Class and SymbolCharacteristicsPrecautions

Class A Compressed Gas

Gas inside cylinder is under pressure The cylinder may explode if heated or damaged Sudden release of high pressure gas streams may puncture skin and cause fatal embolis Transport and handle with care Make sure cylinders are properly secured Store away from sources of heat or fire Use proper regulator

Class B Flammable and Combustible Material

May burn or explode when exposed to heat, sparks or flames Flammable: burns readily at room temperature Combustible: burns when heated Store away from Class C (oxidizing materials) Store away from sources of heat, sparks and flame Do not smoke near these materials

Class C Oxidizing Material

Can cause other materials to burn or explode by providing oxygen May burn skin and eyes on contact Store away from Class B (flammable and combustible) materials Store away from sources of heat and ignition Wear the recommended protective equipment and clothing

Class D Poisonous and Infectious MaterialDivision 1:Materials Causing Immediate and Serious Toxic Effects May cause immediate death or serious injury if inhaled, swallowed, or absorbed through the skin Avoid inhaling gas or vapours Avoid skin and eye contact Wear the recommended protective equipment and clothing Do not eat, drink or smoke near these materials Wash hands after handling

Class D Poisonous and Infectious MaterialDivision 2:Materials Causing Other Toxic Effects May cause death or permanent injury following repeated or long-term exposure May irritate eyes, skin and breathing passages: may lead to chronic lung problems and skin sensitivity May cause liver or kidney damage, cancer, birth defects or sterility Avoid inhaling gas or vapours Avoid skin and eye contact Wear the recommended protective equipment and clothing Do not eat, drink or smoke near these materials Wash hands after handling

Class D Poisonous and Infectious MaterialDivision 3:Biohazardous Infectious Materials Contact with microbiological agents (e.g., bacteria, viruses, fungi and their toxins) may cause illness or death Wear the recommended protective equipment and clothing Work with these materials in designated areas Disinfect area after handling Wash hands after handling

Class E Corrosive Material

Will burn eyes and skin on contact Will burn tissues of respiratory tract if inhaled Store acids and bases in separate areas Avoid inhaling these materials Avoid contact with skin and eyes Wear the recommended protective equipment and clothing

Class F Dangerously Reactive Material

May be unstable, reacting dangerously to jarring, compression, heat or exposure to light May burn, explode or produce dangerous gases when mixed with incompatible materials Store away from heat Avoid shock and friction Wear the recommended protective equipment and clothing

Links to MSDSs can be found at the Environmental Health and Safety web site athttp://www.mcgill.ca/eso/. A glossary explaining the technical and legal terms commonly used in MSDSs ("McGill Material Safety Data Sheet Reference Manual") is available from theEnvironmental Health and Safety.2.2.2 Toxicological properties: LD50AND LC50Despite the limitations of using toxicity data from animal studies to predict the effects on humans, LD50and LC50values often comprise a large part of the available toxicity information, and form the bases for many standards, guidelines and regulations.LD50(Lethal Dose50) is the amount of a substance that, when administered by a defined route of entry (e.g. oral or dermal) over a specified period of time, is expected to cause the death of 50 per cent of a defined animal population. The LD50is usually expressed as milligrams or grams of test substance per kilogram of animal body weight (mg/kg or g/kg).LC50(Lethal Concentration50) is the amount of a substance in air that, when given by inhalation over a specified period of time, is expected to cause the death in 50 per cent of a defined animal population. Some LC50values are determined by administration of test substances to aquatic life in water. TheLC50is expressed as parts of test substance per million parts of air (PPM) for gases and vapours, or as milligrams per litre or cubic metre of air (mg/L or mg/m3) for dusts, mists and fumes.When assessing the hazards of materials used in the laboratory, it is important to remember that substances with lower LD50or LC50values are more toxic that those with higher values.2.2.3 Exposure limits (TLV, PEL)An exposure limit is the maximum limit of exposure to an air contaminant. The threshold limit value (TLV) or permissible exposure limit (PEL) can be expressed as the following: 8-hour time-weighted average (TWA) is the average concentration to which most workers can be exposed during an 8-hour workday, day after day, without harmful effects Short-term exposure limit (STEL), is the maximum average concentration to which most workers can be exposed over a 15 minute period, day after day, without adverse effects Ceiling (C) defines a concentration that must never be exceeded; and is applied to many chemicals with acute toxic effectsIt should be noted that most exposure limits are based on industrial experiences and are not entirely relevant to the laboratory environment. Good laboratory practices and well-designed ventilation systems serve to maintain air concentrations well below these limits.2.2.4 Flash pointThe flash point is the lowest temperature at which a liquid produces enough vapour to ignite in the presence of a source of ignition. The lower the flash point, the greater the risk of fire. Many common laboratory solvents (e.g., acetone, benzene, diethyl ether, methanol) have flash points that are below room temperature.2.2.5 Autoignition temperatureThe ignition or autoignition temperature is the temperature at which a material will ignite, even in the absence of an ignition source; a spark is not necessary for ignition when a flammable vapour reaches its autoignition temperature. The lower the ignition temperature, the greater the potential for a fire started by typical laboratory equipment.2.2.6 Flammable limitsFlammable limits or explosive limits define the range of concentrations of a material in air that will burn or explode in the presence of an ignition source such as a spark or flame. Explosive limits are usually expressed as the percent by volume of the material in air: The lower explosive limit (LEL) or lower flammable limit (LFL) is the lowest vapour concentration that will burn or explode if ignited. Below this limit, the concentration of fuel is too "lean" for ignition, i.e., the mixture is oxygen rich but contains insufficient fuel. The upper explosive limit (UEL) or upper flammable limit (UFL) is the highest vapour concentration that will ignite. Above this limit, the mixture is too "rich" for ignition. The flammable range consists of concentrations between the LEL and UELTable 2 -Flash points, lower explosive limits and exposure limits (8-hour time-weighted averages) of several flammable or combustible laboratory solvents.SolventFPL (oC)LEL (% by volume)Auto ignition temp (C)TLV-TWA * ppm (mg/m3)

acetic acid, glacial394.042710 (25)

acetone-182.5538250 (590)

acetonitrile5.63.052420 (34)

diethyl ether-451.9180400 (1210)**

ethanol, absolute133.34231000 (1900)

ethyl acetate-4.42.0427400 (1440)

methanol116.0464200 (260)

n-pentane-491.5309120 (350)

toluene4.41.1552100 (375)

* NIOSH Pocket Guide to Chemical Hazards, 1999** Pending reviewTOP OF PAGE3. Control of Chemical Hazards3.1 Toxic chemicals and the four routes of entryChemicals can gain entry into the body by: Inhalationof gases, vapours and particulate material (e.g. mists, dusts, smoke, fumes) Absorptionthrough skin of liquids, solids, gases and vapours Ingestionof chemicals directly or indirectly via contaminated foods and beverages and contact between mouth and contaminated hands (nail-biting, smoking) Injectionof chemicals through needles and other contaminated laboratory sharps3.2 Flammable chemicalsFlammable and combustible liquids, solids or gases will ignite when exposed to heat, sparks or flame. Flammable materials burn readily at room temperature, while combustible materials must be heated before they will burn. Flammable liquids or their vapours are the most common fire hazards in laboratories. Refer to Section5.4("Preventing Fires") for specific details on the safe handling of flammable chemicals in the laboratory3.3 Oxidizing chemicalsOxidizers provide oxidizing elements such as oxygen or chlorine, and are capable of igniting flammable and combustible material even in an oxygen-deficient atmosphere (Refer to Section5.1, "The Fire Triangle"). Oxidizing chemicals can increase the speed and intensity of a fire by adding to the oxygen supply, causing materials that would normally not burn to ignite and burn rapidly. Oxidizers can also: React with other chemicals, resulting in release of toxic gases Decompose and liberate toxic gases when heated Burn or irritate skin, eyes, breathing passages and other tissuesPrecautions to follow when using and storing oxidizers in the laboratory include the following: Keep away from flammable and combustible materials Keep containers tightly closed unless otherwise indicated by the supplier Mix and dilute according to the supplier's instructions To prevent release of corrosive dusts, purchase in liquid instead of dry form Reduce reactivity of solutions by diluting with water Wear appropriate skin and eye protection Ensure that oxidizers are compatible with other oxidizers in the same storage area3.4 Reactive chemicals

May be sensitive to jarring, compression, heat or light May react dangerously with water or air May burn, explode or yield flammable or toxic gases when mixed with incompatible materials Can vigorously decompose, polymerize or condense Can also be toxic, corrosive, oxidizing or flammable Some chemicals may not be dangerous when purchased but may develop hazardous properties over time (e.g. diethyl ether and solutions of picric acid).Follow these precautions when working with dangerously reactive chemicals: Understand the hazards associated with these chemicals and use them under conditions which keep them stable Store and handle away from incompatible chemicals Keep water-reactive chemicals away from potential contact with water, such as plumbing, fire sprinkler heads and water baths Handle in a chemical fume hood Wear the appropriate skin and eye protection Work with small quantities Use up or dispose of these chemicals before they attain their expiry date3.5 Corrosive chemicalsCorrosives are materials, such as acids and bases (caustics, alkalis) which can damage body tissues as a result of splashing, inhalation or ingestion. Also: They may damage metals, releasing flammable hydrogen gas They may damage some plastics Some corrosives, such as sulphuric, nitric and perchloric acids, are also oxidizers; thus they are incompatible with flammable or combustible material They may release toxic or explosive products when reacted with other chemicals They may liberate heat when mixed with waterPrecautions for handling corrosive materials include: Wear appropriate skin and eye protection Use in the weakest concentration possible Handle in a chemical fume hood Use secondary containers when transporting and storing corrosives Always dilute by adding acids to water Dilute and mix slowly Store acids separately from gases3.6 Chemical spill response3.6.1 Spill response contingenciesLaboratory heads are responsible for predetermining procedures for response to the types of spill situations that may be anticipated for their operations. Individuals requiring assistance in preparing spill response plans should contactEnvironmental Health and Safety(local 4563).In instances where more extensive equipment or technical assistance is needed, backup can be provided by other internal resources. Communications are handled through the emergency telephone number (Downtown Campuslocal 3000, or Macdonald Campuslocal 7777).3.6.2 Development of spill response plans3.6.2.1 CommunicationsAll laboratories housing hazardous materials are required to provide means of reaching contact people who may be summoned in the event of emergencies involving their laboratory, especially for after-hours situations. This may involve posting the relevant telephone number(s) and/or providing them to the Security Services, who operate the emergency telephone number.Building Directors are also required to provide to the Security Services telephone numbers where they, or alternate contact persons, may be reached during after-hours crises.3.6.2.2 General guidelinesThe following factors are to be considered when developing spill response procedures: Categories of chemicals (e.g. oxidizers, flammable solvents) and their chemical, physical and toxicological properties. The quantities that may be released. Possible locations of release (e.g. laboratory, corridor). Personal protectiveequipment needed. Types and quantities of neutralizing or absorbing material needed.These guidelines should be followed when initially responding to a spill situation: Determine appropriate clean up method by referring to the Material Safety Data Sheet (MSDS). If you are unsure how to proceed, or if you do not have the necessary protective equipment, do not attempt to clean up the spill. If the spill is minor and of known limited danger, clean up immediately. If the spill is of unknown composition, or potentially dangerous (explosive, toxic vapours), alert everyone present and evacuate the room. If the spill cannot be safely handled using the equipment and personnel present, call the emergency telephone number (Downtown Campus local 3000, Macdonald Campus local 7777) to request assistance.3.6.3 Guidelines for specific types of spillsThis section describes how to clean up some of the chemical spills that may occur in the laboratory. Refer to Section6.3.1, "Chemical Waste", for details on how to dispose of the absorbed chemical.3.6.3.1 Flammable and toxic liquids If you can do so without putting yourself at risk, immediately shut off all potential ignition sources If fire occurs, alert everyone present and extinguish all flames. If the fire cannot be controlled immediately pull the nearest fire alarm. If no flames are evident, pour adsorbent around the perimeter of the spill and then cover the rest of the material. Wear an appropriate respirator if toxic vapours are involved. Wear gloves resistant to the chemical being handled. Using a plastic utensil (to avoid creating sparks), scoop up the absorbed spill, place it in a plastic bag, seal it, and place in a labeled container.3.6.3.2 Corrosive liquids Alert everyone present. If vapours are being released, clear the area. Do not attempt to wipe up a corrosive liquid unless it is very dilute. Gloves, boots, apron and eye protection must be used when neutralizing an extensive corrosive spill. Respiratory protection is required if the liquid releases corrosive vapour or gas. Pour the required neutralizing or adsorbing material around the perimeter of the spill, then carefully add water and more neutralizing material to the contained area. Carefully agitate to promote neutralization. Use pH paper to verify that all contaminated areas are neutralized and safe to wipe up. If an adsorbent (eg. spill control pillows) is used instead of a neutralizer, scoop up the absorbed spill, place it in a plastic bag, seal it, and then place in a labeled box. If neutralized material contains no toxic heavy metals (e.g. chromium), flush down the drain with plenty of water.3.6.3.3 Corrosive solidsSmall spills can be cleaned up mechanically with a dustpan and brush. Larger spills should be cleaned up using a HEPA (high-efficiency articulate) filter vacuum. For spills containing fine dusts, an air-purifying respirator with dust filters is recommended, as are gloves, protective goggles, and a lab coat.3.6.3.4 Toxic solidsAvoid disturbing such solids (e.g. asbestos) which may release toxic dusts. Wet the material thoroughly, then place it in a plastic bag and label it appropriately. If wet removal is not possible, a vacuum equipped with a HEPA (High Efficiency Particulate Air) filter is required.3.6.3.5 GasesIn the event of the release of a corrosive gas (e.g. chlorine) or gases that are absorbed through the skin (e.g. hydrogen cyanide), a complete chemical resistant suit and a self-contained breathing apparatus are required. There is no practical means of absorbing or neutralizing a gas - the leak must be corrected at the source.3.6.3.6 MercuryIf a small amount of mercury is spilled (e.g. broken thermometer), use an aspirator bulb or a mercury sponge to pick up droplets, place the mercury in a container, cover with water, seal it, and label the bottle appropriately. To clean up the residual micro-droplets that may have worked into cracks and other hard-to-clean areas, sprinkle sulphur powder or other commercially available product for mercury decontamination. Leave the material for several hours and sweep up solid into a plastic bag, seal it and label it appropriately.Contact theEnvironmental Health and Safety(local 4563)for monitoring of mercury air concentrations.If a large spill of mercury is involved, the area should be closed off, and a mercury respirator worn during the clean-up. A mercury vacuum is available from theHazardousWaste Management(local 5066)for large mercury spills.3.6.3.7 Special categoriesIt is not within the scope of this manual to list procedures for all possible categories of chemicals. For further information on responses to other categories consult the material safety data sheet or contactEnvironmental Health and Safety (local 4563).TOP OF PAGE4. Storage and Handling in Laboratories4.1 General Storage Guidelines Do not block access to emergency safety equipment such as fire extinguishers, eyewashes, showers, first aid kits or utility controls such as breaker boxes or gas shut-off valves Avoid blocking exits or normal paths of travel: keep hallways, walkways and stairs clear of chemicals, boxes, equipment and shelf projections Ensure that the weight of stored material does not exceed the load-bearing capacity of shelves or cabinets Ensure that wall-mounted shelving has heavy-duty brackets and supports and is attached to studs or solid blocking. Regularly inspect clamps, supports, shelf brackets and other shelving hardware Arrange items so that they do not overhang or project beyond the edges of shelves or counter tops Do not stack materials so high that stability is compromised Leave a minimum of 18 inches (45.7 cm) of clearance between sprinkler heads and the top of storage Use a safety step or stepladder to access higher items; never stand on a stool or a chair4.2 Ergonomics Store frequently used items between knee and shoulder height Store heavy objects on lower shelves4.3 Chemical Storage Store hazardous chemicals in an area that is accessible only to authorized laboratory workers Minimize quantities and container sizes kept in the lab Do not store chemicals in aisles, under sinks or on floors, desks or bench tops Store chemicals away from sources of heat (e.g., ovens or steam pipes) and direct sunlight Never stack bottles on top of each other Do not store chemicals above eye level/shoulder height Store larger containers on lower shelves Store liquids inside chemically-resistant secondary containers (such as trays or tubs) that are large enough to hold spills Store chemicals inside closable cabinets or on sturdy shelving that has 12.7 mm-19 mm ( - inch) edge guards to prevent containers from falling Ensure that chemicals cannot fall off the rear of shelves Store chemicals based on compatibility and not in alphabetical order (refer toTable 3andTable 4below). If a chemical presents more than one hazard, segregate according to the primary hazard Designate specific storage areas for each class of chemical, and return reagents to those locations after each use Store volatile toxic and odorous chemicals in a way that prevents release of vapours (e.g., inside closed secondary containers, ventilated cabinets, paraffin sealing) Store flammables requiring refrigeration in explosion-safe or lab-safe refrigerators Label reactive or unstable chemicals (e.g., ethers) with the date of receipt and the date opened Inspect chemicals weekly for signs of deterioration and for label integrity Dispose of unwanted chemicals promptly through Hazardous Waste Management Keep inventory records of chemicals, and update annually4.4 Flammable liquid storage cabinetsFlammable chemicals should be stored inside flammable liquid storage cabinets. Only those flammables in use for the day should be outside the cabinet. Guidelines for cabinet use include: Use NFPA or UL approved flammable liquid storage cabinets Keep cabinet doors of the cabinet closed and latched Do not store other materials in these cabinets4.5 Chemical compatibilityThe storage scheme outlined in Section4.6below ("Chemical Segregation") may not suffice to prevent mixing of incompatible chemicals. Certain hazardous combinations can occur even between chemicals of the same classifications.Table 3shows common examples of incompatible combinations:Table 3 -Examples of incompatible combinations of some commonly used chemicals.CHEMICALKeep from contact with:

Acetic Acidchromic acid, nitric acid, hydroxyl compounds, perchloric acid, peroxides, permanganate

Acetylenechlorine, bromine, copper, fluorine, silver, mercury

Alkali Metals(e.g. Sodium)water, chlorinated hydrocarbons, carbon dioxide, halogens

Ammonia, Anhydrousmercury, chlorine, calcium hypochlorite, iodine, bromine, hydrofluoric acid

Ammonium Nitrateacids, metal powders, flammable liquids, chlorates, nitrites, sulphur, finely divided combustible materials

Anilinenitric acid, hydrogen peroxide

Brominesame as chlorine

Carbon, Activatedcalcium hypochlorite, all oxidizing agents

Chloratesammonium salts, acids, metal powders, sulphur, finely divided combustible materials

Chromic Acidacetic acid, naphthalene, camphor, glycerin, turpentine, alcohol, flammable liquids

Chlorineammonia, acetylene, butadiene, butane, methane, propane (or other petroleum gases), hydrogen, sodium carbide, turpentine, benzene, finely divided metals

Copperacetylene, hydrogen peroxide

Flammable Liquidsammonium nitrate, inorganic acids, hydrogen peroxide, sodium peroxide, halogens

Hydrocarbonsfluorine, chlorine, bromine, chromic acid, sodium peroxide

Hydrofluoric Acidanhydrous ammonia, ammonium hydroxide

Hydrogen Peroxidecopper, chromium, iron, most metals or their salts, alcohols, acetone, aniline, nitromethane, flammable liquids, oxidizing gases

Hydrogen Sulphidefuming nitric acid, oxidizing gases

Iodineacetylene, ammonia (aqueous or anhydrous), hydrogen

Mercuryacetylene, fulminic acid, ammonia

Nitric Acidacetic acid, aniline, chromic acid, hydrocyanic acid, hydrogen sulphide, flammable liquids, flammable gases

Oxalic Acidsilver, mercury

Perchloric Acidacetic anhydride, bismuth and its alloys, organic materials

Potassiumcarbon tetrachloride, carbon dioxide, water

Potassium Chloratesulphuric and other acids

Potassium Permanganateglycerin, ethylene glycol, benzaldehyde, sulphuric acid

Silveracetylene, oxalic acid, tartaric acid, ammonia compounds

Sodium Peroxidealcohol, glacial acetic acid, acetic anhydride, benzaldehyde, carbon disulphide, glycerin, ethylene glycol, ethyl acetate, methyl acetate, furfural

Sulphuric Acidpotassium chlorate, potassium perchlorate, potassium permanganate (or compounds with similar light metals, such as sodium, lithium, etc.)

4.6 Chemical segregation Read the label carefully before storing a chemical. More detailed storage information is usually provided by the MSDS (Material Safety Data Sheet). Ensure that incompatible chemicals are not stored in close proximity to each other. Separate the following types of chemicals from each other according to the segregation scheme in Table 3. Note that this is a simplified scheme and that in some instances chemicals of the same category may be incompatible.For more detailed information refer to the reactivity section of the Material Safety Data Sheet or a reference manual on reactive chemical hazards.Table 4 -Suggested Segregation for Chemical StorageFlammables Store in grounded flammable liquid storage cabinet Separate from oxidizing materialsExamples: Acetone Ethanol Glacial acetic acidNon-flammable solvents Store in cabinet Can be stored with flammable liquids Separate from oxidizing materialsExamples: Carbon tetrachloride Ethylene glycol Mineral oil

Acids Store in cabinet of non-combustible material Separate oxidizing acids from organic acids Separate from caustics, cyanides, sulfidesExamples: Nitric acid Hydrochloric acid Sulphuric acidCaustics Store in dry area Separate from acidsExamples: Ammonium hydroxide Sodium hydroxide Potassium hydroxide

Water reactive chemicals Store in cool, dry location Separate from aqueous solutions Protect from fire sprinkler waterExamples: Sodium Potassium LithiumOxidizers Store in cabinet of non-combustible material Separate from flammable and combustible materialsExamples: Sodium hypochlorite Benzoyl peroxide Potassium permanganate

Non-oxidizing compressed gases Store in well-ventilated area separate physically from oxidizing compressed gasesExamples: Nitrogen Hydrogen Carbon DioxideOxidizing compressed gases Separate physically from flammable compressed gasesExamples: Oxygen Chlorine Nitrous oxide

Non-volatile, non-reactive solids Store in cabinets or open shelves with edge guardsExamples: Agar Sodium chloride Sodium bicarbonate

4.7 Unstable chemicalsMany chemicals, most notably ethers (e.g., THF, dioxane, diethyl and isopropyl ether), are susceptible to decomposition resulting in explosive products. Ethers, liquid paraffins, and olefins form peroxides on exposure to air and light. Since most of these products have been packaged in an air atmosphere, peroxides can form even if the containers have not been opened. Discard unopened containers of ethers after one year Discard containers of ethers within six months of opening Never handle ethers beyond their expiry dates;contact your local waste disposal coordinator to arrange to have the material stabilized and removedThe following are common examples of compounds prone to peroxide formation: Cyclohexene Dicyclopentadiene Diethyl ether (ether) Dimethyl ether Dioxane Isopropyl ether Tetrahydrofuran (THF)

The label and Material Safety Data Sheet (MSDS) will also indicate if a chemical is unstable.4.8 Explosive chemicalsMany chemicals are susceptible to rapid decomposition or explosion when subjected to forces such as being struck, vibrated, agitated or heated. Some become increasingly shock sensitive with age. Picric acid becomes shock sensitive and explosive if it dries out. Refer to the label and the Material Safety Data Sheet to determine if a chemical is explosive. Write the dates received and opened on all containers of explosive or shock-sensitive chemicals Inspect all such containers every month Keep picric acid solutions wet i.e., 30% or more water Discard opened containers after six months, and closed containers after one year, unless the material contains stabilizers Wear appropriate personal protective equipment and perform experiments behind face shield. Work with small quantities.The following are atomic groupings that are associated with the possibility of explosion: acetylide amine oxide azide chlorate diazo diazonium fulminate N-haloamine hypohalite hydroperoxide nitrate nitrite nitroso nitro ozonide perchlorate peroxide picrate

The following are common examples of materials known to be shock-sensitive and explosive: ammonium nitrate ammonium perchlorate copper acetylide dinitrotoluene fulminate of mercury lead azide nitroglycerine picric acid (when dry) trinitrotoluene

TOP OF PAGE5. Fire SafetyLaboratory fires can by caused by bunsen burners, runaway chemical reactions, electrical heating units, failure of unattended or defective equipment, or overloaded electrical circuits. Familiarize yourself with the operation of the fire extinguishers and the location of pull stations, emergency exits and evacuation routes where you work. In the event that the general alarm is sounded use the evacuation routes established for your area and follow the instructions of the Evacuation Monitors. Once outside of the building, move away from the doors to enable others to exit.5.1 The fire triangleFire cannot occur without an ignition source, fuel and an oxidizing atmosphere (usually air), the three elements that comprise what is called the "fire triangle":

Fire will not be initiated if any one of these elements is absent, and will not be sustained if one of these elements is removed. This concept is useful in understanding prevention and control of fires. For example, the coexistence of flammable vapours and ignition sources should be avoided, but when flammable vapours cannot be controlled elimination of ignition sources is essential.5.2 Classes of fireThe National Fire Protection Association (NFPA) has defined four classes of fire, according to the type of fuel involved. These are: Class Afires involve combustibles such as paper, wood, cloth, rubber and many plastics. Class Bfires entail burning of liquid fuels like oil-based paints, greases, solvents, oil and gasoline. Class Cfires are of electrical origin (fuse boxes, electric motors, wiring). Class Dfires encompass combustible metals such as magnesium, sodium, potassium and phosphorus.5.3 Fire extinguishersFire extinguishers are rated as A, B, C or D (or combinations of A, B, C and D) for use against the different classes of fires. Familiarize yourself with the fire class ratings of the extinguishers in your work area so that you will know what types of fire you can attempt to extinguish with them.Learn how to use the extinguisher in your lab, as there will be no time to read instructions during an emergency. Attempt to fight small fires only, and only if there is an escape route behind you. Remember to have the extinguisher recharged after every use:inform Building Services at local 4560 (local 7828 at Macdonald Campus). If you do fight a fire, remember the acronym "PASS" when using the extinguisher: P: Pull and twist the locking pin to break the seal. A: Aim low, and point the nozzle at the base of the fire. S: Squeeze the handle to release the extinguishing agent. S: Sweep from side to side until the fire is out. Be prepared to repeat the process if the fire breaks out again5.4 Preventing firesUse the following precautions when working with or using flammable chemicals in a laboratory; keep in mind that these precautions also apply to flammable chemical waste. Minimize the quantities of flammable liquids kept in the laboratory. Do not exceed the maximum container sizes specified by the National Fire Protection Association (NFPA), as listed inAppendix 1. Except for the quantities needed for the work at hand, keep all flammable liquids in NFPA- or UL- (Underwriter's Laboratories) approved flammable liquid storage cabinets. Keep cabinet doors closed and latched at all times. Do not store other materials in these cabinets. Use and store flammable liquids and gases only in well-ventilated areas. Use a fume hood when working with products that release flammable vapours. Keep flammable solvent containers, including those for collecting waste, well capped. Place open reservoirs or collection vessels for organic procedures like HPLC inside vented chambers. Store flammable chemicals that require refrigeration in "explosion-safe" (non-sparking) laboratory refrigerators. Keep flammable chemicals away from ignition sources, such as heat, sparks, flames and direct sunlight. Avoid welding or soldering in the vicinity of flammables. Bond and ground large metal containers of flammable liquids in storage. To avoid the build-up of static charges, bond containers to each other when dispensing. Use portable safety cans for storing, dispensing and transporting flammable liquids. Clean spills of flammable liquids promptly.5.5 EvacuationsIn the event that the general alarm is sounded, follow the evacuation routes established for your area; do not use the elevators. Follow the instructions of the Evacuation Monitors. Once outside the building, move away from the doors to allow others to exit.TOP OF PAGE6. Hazardous Waste Disposal6.1 Waste minimizationIn order to minimize the amount of hazardous waste presented for disposal, it is important to follow these guidelines: Avoid overstocking: one of the main sources of laboratory waste is surplus stock - the result of over buying. Recent pricing arrangements with suppliers have greatly reduced the benefits of purchasing chemicals in large volumes. Also, there is little need to store large quantities of chemicals, as orders are generally shipped the day after an order is received. Do not accept donations of materialsthat you don't plan to use. Many companies have traditionally unloaded unwanted reagents by donating them to laboratories, which eventually transfers the cost of disposal to the University. Substitute hazardous experimental materialsfor non-hazardous ones. For example, use aqueous-based, biodegradable scintillation fluids whenever possible.6.2 Hazardous waste disposal guidelines Label all waste materials completely and legibly, using labels available fromHazardous Waste Management(HWM, local 5066). Inadequately labeled containers will not be accepted. Package waste materials in approved containers, available from HWM. Over filled and/or leaking containers cannot be accepted for disposal. Never discharge wastes into the sewer unless you have verified that hazardous wastes regulations permit you to do so. For information, contact HWM6.3 Waste preparation procedures6.3.1 Chemical waste6.3.1.1 Organic solvents and oils Collect in the containers provided byHazardous Waste Management(HWM, local 5066). Indicate the composition of the contents as accurately as possible on the attached label.6.3.1.2 Miscellaneous chemicals and cylinders Complete the lab chemical inventory form and send toHWM (fax 4633). Await instructions.6.3.1.3 Chemicals of unknown composition Unknown chemicals cannot be accepted. Analyze or contact HWM to arrange for analysis (at the expense of the waste generator).6.3.1.4 Peroxide-forming (e.g. ether) and explosive (e.g. dry picric acid) chemicals Do not mix with solvents or other waste. If the material is older than one year, do not attempt to open or move the container. Contact HWM for advice.6.3.1.5. Corrosives (acids and bases) Collect acids (pH7) separately in the plastic containers provided by HWM. Do not mix acids with bases. Indicate the composition of the contents, as accurately as possible, on the attached label.6.3.2 Biomedical waste6.3.2.1 Animal carcasses Place in the plastic-lined biomedical waste containers provided byHazardous Waste Management (HWM, local 5066) Ensure that the weight of individual containers does not exceed 40 pounds. Store in a refrigerated area.6.3.2.2 Infectious laboratory waste Place in the plastic-lined biomedical containers provided by WMP.6.3.2.3 Biohazardous sharpsRefer to Section6.3.3.1below for further details.6.3.2.4 Blood and blood-contaminated materials Unclotted blood can be disposed of via the sanitary drains. Designate one sink for this purpose. After discharging blood, decontaminate the sink with a 5-10% dilution of household bleach. Allow a contact time of 20 minutes, then rinse with water. Dispose of blood-contaminated materials as infectious laboratory waste.6.3.3 Sharps6.3.3.1 Definition of SharpsSharps are defined as any material that can penetrate plastic bags: examples include syringe needles, scalpel blades, glass and plastic pipettes, disposable pipette tips, etc.6.3.3.1.1Contaminated sharps Label a plastic, puncture proof container (e.g. empty liquid bleach bottle) with the word "SHARPS", the appropriate hazard warning symbol (e.g. biohazard, radioactive) and the name of the Principal Investigator. Discard containers of sharps contaminated with infectious materials into biomedical waste containers as per the procedure for Infectious Laboratory Waste (Section6.3.2.2). Discard containers of sharps contaminated with radioactive materials as per the procedure for solid radioactive waste (Section6.3.4.1)6.3.3.1.2Non-contaminated sharps Label a puncture-proof container (wide-mouth plastic bottle or a heavy-duty cardboard box lined with plastic) with the word "SHARPS", and the name of the Principal Investigator. Accumulate in the designated container, without overfilling. When full, close and seal the container and place it beside the regular garbage receptacle for pickup by the cleaning staff.6.3.3.2 Broken glassware (uncontaminated) Designate a cardboard box for broken glass; label it "BROKEN GLASS", and place glass inside. When the box is full, seal it with tape and place it next to the garbage receptacle for pickup by the cleaning staff.6.3.3.3 Empty chemical reagent bottles Remove the cap from the empty bottle and allow volatile materials to evaporate into the fume hood. Rinse the bottle three times with tap water and let dry. Remove or obliterate the label. Place the uncapped bottle next to the garbage receptacle.6.3.4 Radioactive waste6.3.4.1 Solid waste (except sealed sources) Whenever possible, package alpha emitting radioisotopes separately from other radioisotopes. Whenever possible, package long-lived (half life > 10 years) radioisotopes separately from short-lived radioisotopes. Accumulate wastes in the solid radioactive waste containers provided. Update the information on the label as wastes are placed in the container.6.3.4.2 Sealed and encapsulated sources Do not package sealed sources with other types of waste materials. Contact your local Hazardous Waste Coordinator.6.3.4.3 Liquid scintillation vials Leave fluids in their vials. Deposit vials into the designated 45-gallon drum in your building's waste storage area and enter the required information on the inventory sheet attached to the drum.6.3.4.4 Liquid radioactive waste Aqueous liquid wastes at or below 0.01 scheduled quantity per litre can be disposed of via the regular drainN.B. It is illegal to dilute for the purposes of reducing radioactivity to below this level. Consult with the Radiation Safety Officer if additional assistance is required in determining scheduled quantities. Containers are available for laboratories that are unable to avoid the generation of liquid radioactive wastes. In order to control costs, you are asked to exercise great care to fill the containers with only such materials.TOP OF PAGE7. Laboratory Ventilation And Fume Hoods7.1 General ventilationGeneral ventilation, also called dilution ventilation, involves dilution of inside air with fresh outside air, and is used to: maintain comfortable temperature, humidity and air movement for room occupants dilute indoor air contaminants replace air as it is exhausted to the outside via local ventilation devices such as fume hoods provide a controlled environment for specialized areas such as surgery or computer roomsGeneral ventilation systems comprise an air supply and an air exhaust. The air may be supplied via a central HVAC (Heating, Ventilation and Air Conditioning) system or, especially in older buildings, via openable windows. Laboratory air may be exhausted through either local exhaust devices or air returns connected to the HVAC system.7.2 Local ventilation devicesLocal exhaust ventilation systems capture and discharge air contaminants (biological, chemical, radioactive) or heat from points of release. Common local exhaust ventilation devices found in laboratories include: chemical fume hoods canopy hoods slotted hoods biological safety cabinets direct connections7.2.1 Chemical fume hoodsChemical fume hoods are enclosed units with a sliding sash for opening or closing the hood. They are able to capture and exhaust even heavy vapours, and are preferred for all laboratory procedures that require manual handling of hazardous chemical material. Refer to Section7.4below for information on the safe use of chemical fume hoods.7.2.2 Canopy hoodsCanopy hoods are designed to capture heat from processes or equipment, such as atomic absorption spectrophotometers or autoclaves; a canopy or bonnet is suspended over a process and connected to an exhaust vent. The following limitations make canopy hoods poor substitutes for chemical fume hoods, because they: draw contaminated air through the user's breathing zone do not capture heavy vapours provide less containment than chemical fume hoods, and are more affected by air turbulence do not provide adequate suction more than a few inches away from the hood opening7.2.3 Slotted hoodsSlotted hoods, or benches, have one or more narrow horizontal openings, or slots, at the back of the work surface; the slots are connected to exhaust ducting. These special purpose hoods are used for work with chemicals of low to moderate toxicity only, such as developing black and white photographs.7.2.4 Biological safety cabinetsBiological safety cabinets are for use with biological material; depending on the cabinet class, they provide protection of the environment, user and/or product. They are not recommended for use with hazardous chemicals because most models recirculate air into the laboratory, and because the HEPA filter that is integral to the protective function can be damaged by some chemicals. Biological safety cabinets are described in more detail in the McGill Laboratory Biosafety Manual.7.2.5 Direct connectionsDirect connections provide direct exhausting of contaminants to the outdoors and are used for venting: flammable liquid storage cabinets other toxic chemical storage cabinets solvent and waste reservoirs, such as for HPLC solvent systems reaction vessels, sample analyzers, ovens, dryers and vacuum pump outlets7.3 Ventilation balancing and containmentBy regulation, more air is exhausted from a laboratory than is supplied to it, resulting in a net negative pressure (vacuum) in the laboratory. Negative pressure draws air into the laboratory from surrounding areas, and serves to prevent airborne hazardous chemicals, radiation or infectious microorganisms from spreading outside the laboratory in the event of an accidental release inside the laboratory. Balancing of laboratory ventilation must take into consideration the amount of air exhausted by local ventilation devices such as fume hoods. Modern laboratories do not have operable windows, as opening of windows tends to pressurize a room, pushing air from the laboratory into adjacent non-laboratory areas.7.4 Safe use of chemical fume hoodsFume hoods properly used and maintained, will render substantial protection, provided the user is aware of its capabilities and limitations. The performance standard for fume hoods is the delivery of a minimum face velocity of 100 linear feet per minute at half sash height. An anemometer for determining a fumehood's face velocity is available from Environmental Health and Safety. To ensure your fume hood provides the highest degree of protection observe the following guidelines:1. Only materials being used in an ongoing experiment should be kept in the fume hood. Cluttering the hood will create air flow disturbances.2. When it is necessary to keep a large apparatus inside a hood, it should be placed upon blocks or legs to allow air to flow underneath.3. Operate the hood with the sash as low as practical. Reducing the open face will increase the face velocity.4. Work as far into the hood as possible. At least six inches is recommended.5. Do not lean into the hood. This disturbs the air flow, and also places your head into the contaminated air inside the hood.6. Do not make quick motions into or out of the hood, or create cross drafts by walking rapidly past the hood. Opening doors or windows can sometimes cause strong air currents which will disturb the air flow into the hood.7. Heating devices should be placed at the rear of the hood.8. Do not use a hood for any function it was not specifically designed, such as perchloric acid, some radioisotopes, etc.9. Keep hood door closed when not attended.10. Remember that sinks inside fume hoods are not designed for disposing of chemical wastes.TOP OF PAGE8. Compressed Gases and Cryogenics8.1 Hazards of compressed gasesCompressed gases are hazardous due to the high pressure inside cylinders. Knocking over an unsecured, uncapped cylinder of compressed gas can break the cylinder valve; the resulting rapid escape of high pressure gas can turn a cylinder into an uncontrolled rocket or pinwheel, causing serious injury and damage. Poorly controlled release of compressed gas in the laboratory can burst reaction vessels, cause leaks in equipment and hoses or result in runaway chemical reactions. Compressed gases may also have flammable, oxidizing, dangerously reactive, corrosive or toxic properties. Inert gases such as nitrogen, argon, helium and neon can displace air, reducing oxygen levels in poorly ventilated areas and causing asphyxiation.8.2 Safe handling, storage and transport of compressed gas cylinders All gas cylinders, full or empty, should be securely supported using suitable racks, straps, chains or stands. When cylinders are not in use or are being transported, remove the regulator and attach the protective cap. An appropriate cylinder cart should be used for transporting cylinders. Chain or strap the cylinder to the cart. Verify that the regulator is appropriate for the gas being used and the pressure being delivered. Do not rely upon the pressure gauge to indicate the maximum pressure ratings; check the regulator's specifications. Do not use adaptors or Teflon tape to attach regulators to gas cylinders. Never bleed a cylinder completely empty; leave a residual pressure. Do not lubricate the high-pressure side of an oxygen regulator. Do not expose cylinders to temperature extremes. Store incompatible classes of gases separately.8.3 Cryogenic hazardsCryogenics are very low temperature materials such as dry ice (solid CO2) and liquefied air or gases like nitrogen, oxygen, helium, argon and neon. The following hazards are associated with the use of cryogenics: asphyxiation due to displacement of oxygen (does not apply to liquid air and oxygen) embrittlement of materials from extreme cold frostbite explosion due to pressure build up condensation of oxygen and fuel (e.g. hydrogen and hydrocarbons) resulting in explosive mixtures8.4 Cryogenic handling precautionsThe following are precautions for handling cryogenics: Control ice build up Use only low-pressure containers equipped with pressure-relief devices. Protect skin and eyes from contact; wear eye protection and insulated gloves. Use and store in well-ventilated areas. Keep away from sparks or flames. Use materials resistant to embrittlement (e.g. latex rubber tubing). Watches, rings, bracelets or other jewelry that could trap fluids against flesh should not be worn when handling cryogenic liquids To prevent thermal expansion of contents and rupture of the vessel, do not fill containers to more than 80% of capacity. If cryogens must be transported by elevator, take adequate precautions to prevent possible injury. Send cryogenic liquid tanks in elevators without any passengers and ensure that nobody gets on the elevator while the cryogen is being transported.TOP OF PAGE9. Physical Hazards and Ergonomics9.1 Electrical safety Purchase and use only CSA-approved electrical equipment. All electrical outlets should carry a grounding connection requiring a three-pronged plug. Never remove the ground pin of a three-pronged plug. Remove cords by grasping the plug, not the cord. All electrical equipment (except glass-cloth heaters and certain models of oscillographs requiring a floating ground) should be wired with a grounding plug. All wiring should be done by, or under the approval of, a licensed electrician. Electrical equipment that has been wetted should be disconnected at the main switch or breaker before being handled. Familiarize yourself with the location of such devices. Know how to cut off the electrical supply to the laboratory in the event of an emergency. Maintain free access to panels; breaker panels should be clearly labeled as to which equipment they control. Ensure that all wires are dry before plugging into circuits. Electrical equipment with frayed wires should be repaired before being put into operation. Tag and disconnect defective equipment. Be sure that all electrical potential has been discharged before commencing repair work on any equipment containing high voltage power supplies or capacitors. Minimize the use of extension cords and avoid placing them across areas of pedestrian traffic. Use only C02, halon, or dry chemical fire extinguishers for electrical fires. Use ground fault circuit interrupters for all electrical equipment used for administering electrical current to human subjects or measuring electrical signals from human subjects.9.2 High pressure and vacuum workPressure differences between equipment and the atmosphere result in many lab accidents. Glass vessels under vacuum or pressure can implode or explode, resulting in cuts from projectiles and splashes to the skin and eyes. Glass can rupture even under small pressure differences. Rapid temperature changes, such as those that occur when removing containers from liquid cryogenics, can lead to pressure differences, as can carrying out chemical reactions inside sealed containers.The hazards associated with pressure work can be reduced by: checking for flaws such as cracks, scratches and etching marks before using vacuum apparatus using vessels specifically designed for vacuum work. Thin-walled or round-bottomed flasks larger than 1 L should never be evacuated assembling vacuum apparatus so as to avoid strain. Heavy apparatus should be supported from below as well as by the neck taping glass vacuum apparatus to minimize projectiles due to implosion using adequate shielding when conducting pressure and vacuum operations allowing pressure to return to atmospheric before opening vacuum desiccators or after removal of a sample container from cryogenics wearing eye and face protection when handling vacuum or pressure apparatus9.3 Repetitive work and ergonomicsErgonomics is concerned with how the workplace "fits" the worker. Performing certain work tasks without regard for ergonomic principles can result in: fatigue repetitive motion injuries strains, aches and injuries from biomechanical stresses eyestrain from video display terminals (VDTs) decreased moraleFactors that can increase the risk of musculoskeletal injury are: awkward positions or movements repetitive movements application of forceIn a laboratory setting, look for the following when addressing ergonomic concerns: Laboratory bench and workbench heights are suitable for all personnel Laboratory chairs are on wheels or castors, are sturdy (5-legged), and are adjustable (seat height, angle, backrest height) VDTs are positioned at or slightly below eye level, and are positioned so as to avoid glare from lights or windows Computer keyboards and pointing devices are positioned so that wrists are kept in a neutral position and forearms are horizontal Colour, lettering size and contrast of equipment display monitors are optimized so as not to cause eye strain Work station design does not necessitate excessive bending, reaching, stretching or twisting Vibration-producing equipment, such as vortex mixers and pump-type pipettors are not used for extended periods of time Buttons and knobs on equipment are accessible and of a good size Heavy items are not carried or handled Laboratory workers are using proper techniques when lifting or moving materials Indoor air quality parameters, such as temperature, humidity and air supply are comfortable Floors are slip-resistant Noise levels are not excessive9.4 Glassware safety Use a dustpan and brush, not your hands, to pick up broken glass. Discard broken glass in a rigid container separate from regular garbage and label it appropriately (see Waste Preparation Procedures, Section6.3). Protect glass that is subject to high pressure or vacuum. Wrapping glass vessels with cloth tape will minimize the possibility of projectiles. Glass is weakened by everyday stresses such as heating and bumping. Handle used glassware with extra care. Discard or repair all damaged glassware, as chipped, cracked or star-cracked vessels cannot handle the normal stresses.When handling glass rods or tubes: fire polish the ends, lubricate with water or glycerine when inserting through stopper, ensure stopper holes are properly sized, and not too small, insert carefully, with a slight twisting motion, keeping hands close together, and use gloves or a cloth towel to protect your handsTOP OF PAGE10. Equipment SafetyWhenever lab equipment is purchased, preference should be given to equipment that limits contact between the operator and hazardous material, and mechanical and electrical energy is corrosion-resistant, easy to decontaminate and impermeable to liquids has no sharp edges or burrsEvery effort should be made to prevent equipment from becoming contaminated. To reduce the likelihood of equipment malfunction that could result in leakage, spill or unnecessary generation of aerosolized pathogens: Review the manufacturer's documentation. Keep for future reference. Use and service equipment according to the manufacturer's instructions. Ensure that anyone who uses a specific instrument or piece of equipment is properly trained in setup, use and cleaning of the item. Ensure that equipment leaving the laboratory for servicing or disposal is appropriately decontaminated. Complete aCertificate Of Equipment Decontamination[.pdf] form and attach it to the equipment before it leaves the lab.The following sections outline some of the precautions and procedures to be observed with some commonly used laboratory equipment.10.1 CentrifugesImproperly used or maintained centrifuges can present significant hazards to users. Failed mechanical parts can result in release of flying objects, hazardous chemicals and biohazardous aerosols. The high speed spins generated by centrifuges can create large amounts of aerosol if a spill, leak or tube breakage occurs. To avoid contaminating your centrifuge: Check glass and plastic centrifuge tubes for stresslines, hairline cracks and chipped rims before use. Use unbreakable tubes whenever possible. Avoid filling tubes to the rim. Use caps or stoppers on centrifuge tubes. Avoid using lightweight materials such as aluminum foil as caps. Use sealed centrifuge buckets (safety cups) or rotors that can be loaded and unloaded in a biological safety cabinet. Decontaminate the outside of the cups or buckets before and after centrifugation. Inspect o-rings regularly and replace if cracked or dry. Ensure that the centrifuge is properly balanced. Do not open the lid during or immediately after operation, attempt to stop a spinning rotor by hand or with an object, or interfere with the interlock safety device. Decant supernatants carefully and avoid vigorous shaking when resuspending.When using high-speed or ultra centrifuges, additional practices should include: Connect the vacuum pump exhaust to a trap. Record each run in a logbook: keep a record of speed and run time for each rotor. Install a HEPA filter between the centrifuge and the vacuum pump when working with biohazardous material. Never exceed the specified speed limitations of the rotor.10.2 Electrophoresis equipment Ensure that electrophoresis equipment is properly grounded and has electrical interlocks. Do not bypass safety interlocks. Inspect electrophoresis equipment regularly for damage and potential tank leaks. Locate equipment away from high traffic areas, and away from wet areas such as sinks or washing apparatus. Display warning signs.10.3 Heating baths, water bathsHeating baths keep immersed materials immersed at a constant temperature. They may be filled with a variety of materials, depending on the bath temperature required; they may contain water, mineral oil, glycerin, paraffin or silicone oils, with bath temperatures ranging up to 300oC. The following precautions are appropriate for heating baths: set up on a stable surface, away from flammable and combustible materials including wood and paper relocate only after the liquid inside has cooled ensure baths are equipped with redundant heat controls or automatic cutoffs that will turn off the power if the temperature exceeds a preset limit use with the thermostat set well below the flash point of the heating liquid in use equip with a thermometer to allow a visual check of the bath temperature.The most common heating bath used in laboratories is the water bath. When using a water bath: clean regularly; a disinfectant, such as a phenolic detergent, can be added to the water avoid using sodium azide to prevent growth of microorganisms; sodium azide forms explosive compounds with some metals raise the temperature to 90oC or higher for 30 minutes once a week for decontamination purposes unplug the unit before filling or emptying, and have the continuity-to-ground checked regularly10.4 Shakers, blenders and sonicatorsWhen used with infectious agents, mixing equipment such as shakers, blenders, sonicators, grinders and homogenizers can release significant amounts of hazardous aerosols, and should be operated inside a biological safety cabinet whenever possible. Equipment such as blenders and stirrers can also produce large amounts of flammable vapours. The hazards associated with this type of equipment can be minimized by: selecting and purchasing equipment with safety features that minimize leaking selecting and purchasing mixing apparatus with non-sparking motors. checking integrity of gaskets, caps and bottles before using. Discard damaged items. allowing aerosols to settle for at least one minute before opening containers covering tops of blenders with a disinfectant-soaked towel during operation, when using biohazardous material when using a sonicator, immersing the tip deeply enough into the solution to avoid creation of aerosols decontaminating exposed surfaces after use10.5 Ovens and hot platesLaboratory ovens are useful for baking or curing material, off-gassing, dehydrating samples and drying glassware. Select and purchase an oven whose design prevents contact between flammable vapours and heating elements or spark-producing components Discontinue use of any oven whose backup thermostat, pilot light or temperature controller has failed Avoid heating toxic materials in an oven unless it is vented outdoors (via a canopy hood, for example) Never use laboratory ovens for preparation of food for human consumption Glassware that has been rinsed with an organic solvent should be rinsed with distilled water before it is placed in a drying oven10.6 Analytical equipmentThe following instructions for safe use of analytical equipment are general guidelines; consult the user's manual for more detailed information on the specific hazards: Ensure that installation, modification and repairs of analytical equipment are carried out by authorized service personnel. Read and understand the manufacturer's instructions before using this equipment. Make sure that preventive maintenance procedures are performed as required. Do not attempt to defeat safety interlocks. Wear safety glasses and lab coats (and other appropriate personal protective equipment as specified) for all procedures.10.6.1 Scintillation counters Use sample vials that meet the manufacturer's specifications Keep counters clean and free of foreign material To avoid contaminating the counter and its accessories with radioactivity, change gloves before loading racks in the counter or using the computer keyboard. Verify on a regular basis (by wipe testing) that the equipment has not become contaminated.10.6.2 Atomic absorption (AA) spectrometersSample preparation for atomic absorption procedures often require handling of flammable, toxic and corrosive products. Familiarize yourself with the physical, chemical and toxicological properties of these materials and follow the recommended safety precautions. Atomic absorption equipment must be adequately vented, as toxic gases, fumes and vapours are emitted during operation. Other recommendations to follow when carrying out atomic absorption analysis are: Wear safety glasses for mechanical protection. Check the integrity of the burner, drain and gas systems before use. Inspect the drain system regularly; empty the drain bottle frequently when running organic solvents. Allow the burner head to cool to room temperature before handling. Never leave the flame unattended. A fire extinguisher should be located nearby. Avoid viewing the flame or furnace during atomization unless wearing protective eyewear. Hollow cathode lamps are under negative pressure and should be handled with care and disposed of properly to minimize implosion risks.10.6.3 Mass spectrometers (MS)Mass spectrometry requires the handling of compressed gases and flammable and toxic chemicals. Consult MSDSs for products before using them. Specific precautions for working with the mass spectrometer include: Avoid contact with heated parts while the mass spectrometer is in operation. Verify gas, pump, exhaust and drain system tubing and connections before each use. Ensure that pumps are vented outside the laboratory, as pump exhaust may contain traces of the samples being analyzed, solvents and reagent gas. Used pump oil may also contain traces of analytes and should be handled as hazardous waste.10.6.4 Gas chromatographs (GC)Gas chromatography requires handling compressed gases (nitrogen, hydrogen, argon, helium), and flammable and toxic chemicals. Consult product MSDSs before using such hazardous products. Specific precautions for working with gas chromatographs include: Perform periodic visual inspections and pressure leak tests of the sampling system plumbing, fittings and valves. Follow the manufacturer's instructions when installing columns. Glass or fused capillary columns are fragile: handle them with care and wear safety glasses to protect eyes from flying particles while handling, cutting or installing capillary columns. Turn off and allow heated areas such as the oven, inlet and detector, as well as connected hardware, to cool down before touching them. To avoid electrical shock, turn off the instrument and disconnect the power cord at its receptacle whenever the access panel is removed. Turn off the hydrogen gas supply at its source when changing columns or servicing the instrument. When using hydrogen as fuel (flame ionization FID and nitrogen-phosphorus detectors NPD), ensure that a column or cap is connected to the inlet fitting whenever hydrogen is supplied to the instrument to avoid buildup of explosive hydrogen gas in the oven. Measure hydrogen gas and air separately when determining gas flow rates. Perform a radioactive leak test (wipe test) on electron capture detectors (ECDs) at least every 6 months for sources of 50MBq (1.35 mCi) or greater. Ensure that the exhaust from (ECDs) is vented to the outside. When performing split sampling, connect the split vent to an exhaust ventilation system or appropriate chemical trap if toxic materials are analyzed or hydrogen is used as the carrier gas. Use only helium or nitrogen gas, never hydrogen, to condition a chemical trap.10.6.5 Nuclear magnetic resonance (NMR) equipmentThe superconducting magnet of NMR equipment produces strong magnetic and electromagnetic fields that can interfere with the function of cardiac pacemakers. Users of pacemakers and other implanted ferromagnetic medical devices are advised to consult with their physician, the pacemaker's manual and pacemaker manufacturer before entering facilities which house NMR equipment. Precautions for work with NMR include the following: Post clearly visible warning signs in areas with strong magnetic fields. Measure stray fields with a gaussmeter, and restrict public access to areas of 5-gauss or higher. The strong magnetic field can suddenly pull nearby unrestrained magnetic objects into the magnet with considerable force. Keep all tools, equipment and personal items containing ferromagnetic material (e.g., steel, iron) at least 2 metres away from the magnet. Though not a safety issue, advise users that the magnetic field can erase magnetic media such as tapes and floppy disks, disable credit and automated teller machine (ATM) cards, and damage analog watches. Avoid skin contact with cryogenic (liquid) helium and nitrogen; wear a protective face mask and loose-fitting thermal gloves during dewar servicing and when handling frozen samples. Refer to Section11, "Compressed Gases and Cryogenics". Ensure that ventilation is sufficient to remove the helium or nitrogen gas exhausted by the instrument. Avoid positioning your head over the helium and nitrogen exit tubes. NMR tubes are thin-walled; handle them carefully and reserve them for NMR use only.10.6.6 High-pressure liquid chromatography (HPLC) equipmentHPLC procedures may require handling of compressed gas (helium) and flammable and toxic chemicals. Familiarize yourself with the hazardous properties of these products, as well as recommended precautionary measures, by referring to MSDSs. Inspect the drain system regularly; empty the waste container frequently when using organic solvents. Ensure that waste collection vessels are vented. Never use solvents with autoignition temperatures below 110oC. Be sure to use a heavy walled flask if you plan to use vacuum to degas the solvent. Never clean a flowcell by forcing solvents through a syringe: syringes under pressure can leak or rupture, resulting in sudden release of syringe contents. High voltage and internal moving parts are present in the pump. Switch off the electrical power and disconnect the line cord when performing routine maintenance of the pump. Shut down and allow the system to return to atmospheric pressure before carrying out maintenance procedures.10.6.7 Liquid chromatography (LC/MS) equipmentLC/MS requires the handling of compressed nitrogen and flammable and toxic