Chemical Proteomics: Methods and ProtocolsDrewes, Gerard

ISBN-13: 9781617793639

Table of Contents

Editors: Marcus Bantscheff & Gerard Drewes

Contents

Preface

Contributors

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Part I: Introduction

1       Mass Spectrometry-Based Chemoproteomic Approaches

Marcus Bantscheff

ÂÂ

2. Chemical Proteomics in Drug Discovery

Gerard Drewes

Part II: Small Molecules and Probe Design

ÂÂ

3. Compound Immobilization and Drug-Affinity Chromatography

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

ÂÂ

4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

ÂÂ

5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Gerard Drewes

Part II: Small Molecules and Probe Design

ÂÂ

3. Compound Immobilization and Drug-Affinity Chromatography

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

ÂÂ

4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

ÂÂ

5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

ÂÂ

4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

ÂÂ

5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Gerard Drewes

Part II: Small Molecules and Probe Design

ÂÂ

3. Compound Immobilization and Drug-Affinity Chromatography

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

ÂÂ

4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

ÂÂ

5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

ÂÂ

4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

ÂÂ

5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Xia Ge and Daniel S. Sem

ÂÂ

6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

ÂÂ

7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture Compound MassSpectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

ÂÂ

Part III: Target Discovery and Target Validation

ÂÂ

9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

ÂÂ

15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

ÂÂ

19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

ÂÂ

20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

ÂÂ

ÂÂ

ÂÂ

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

ÂÂ

10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI MassSpectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Ming-Qiang Zhang

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Gerard Drewes

Part II: Small Molecules and Probe Design

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3. Compound Immobilization and Drug-Affinity Chromatography

Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

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4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

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5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

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4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

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5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Ming-Qiang Zhang

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Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

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4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

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5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

ÂÂ

8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture Compound MassSpectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI Mass

Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Ming-Qiang Zhang

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Uwe Rix, Manuela Gridling, and Giulio Superti-Furga

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4. Affinity-based Chemoproteomics with Small Molecule-Peptide Conjugates

Chaitanya Saxena

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5. A Chemical Proteomic Probe for Detecting Dehydrogenases: Catechol Rhodanine

Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture CompoundMass Spectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture Compound MassSpectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI MassSpectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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6. Probing Proteomes with Benzophenone Photoprobes

Akira Kawamura and Doina M. Mihai

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7. Biotinylated Probes for the Analysis of Protein Modification by Electrophiles

Simona G. Codreanu, Hye-Young H. Kim, Ned A. Porter, and Daniel C. Liebler

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8. Profiling of Methyltransferases and Other S-adenosyl-l-homocysteine-Binding Proteins by Capture Compound MassSpectrometry (CCMS)

Thomas Lenz, Peter Poot, Elmar Weinhold, and Mathias Dreger

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Part III: Target Discovery and Target Validation

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9. Identifying Cellular Targets of Small-Molecule Probes and Drugs with Biochemical Enrichment and SILAC

Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric MassTags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

ÂÂ

11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

ÂÂ

12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

ÂÂ

13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

ÂÂ

14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

ÂÂ

16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

ÂÂ

Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

ÂÂ

18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI MassSpectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Ming-Qiang Zhang

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Shao-En Ong, Xiaoyu Li, Monica Schenone, Stuart L. Schreiber, and Steven A. Carr

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10. Determination of Kinase Inhibitor Potencies in Cell Extracts by Competition Binding Assays and Isobaric Mass Tags

Carsten Hopf, Dirk Eberhard, Markus Boesche, Sonja Bastuck, Birgit Dümpelfeld, and Marcus Bantscheff

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11. Affinity-Based Profiling of Dehydrogenase Subproteomes

Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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Xia Ge and Daniel S. Sem

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Mathias Q. Müller and Andrea Sinz

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Natalia Gasilova, Alexis Nazabal

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Amy Hye Won Jeon, Gerold Schmitt-Ulms

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17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Natalia Gasilova, Alexis Nazabal

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Amy Hye Won Jeon, Gerold Schmitt-Ulms

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17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Xia Ge and Daniel S. Sem

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Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI MassSpectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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12. Probing the Specificity of Protein-Protein Interactions by Quantitative Chemical Proteomics

Duangnapa Kovanich, Thin Thin Aye, Albert J.R. Heck, and Arjen Scholten

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13. Fluorescence-Based Proteasome Activity Profiling

Annemieke de Jong, Karianne G. Schuurman, Boris Rodenko, Huib Ovaa, and Celia R. Berkers

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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20. Working with Small Molecules: Rules-of-Thumb of “Drug Likenessâ€Â

Ming-Qiang Zhang

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDI MassSpectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Ming-Qiang Zhang

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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19. A Database for Chemical Proteomics - ChEBI

Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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14. Chemical Cross-Linking and High-Resolution Mass Spectrometry to Study Protein-Drug Interactions

Mathias Q. Müller and Andrea Sinz

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15. Monitoring Ligand Modulation of Protein-Protein Interactions by Chemical Cross-Linking and High-Mass MALDIMass Spectrometry

Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of Kinetic Solubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Natalia Gasilova, Alexis Nazabal

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16. Time-Controlled Transcardiac Perfusion Crosslinking for In Vivo Interactome Studies

Amy Hye Won Jeon, Gerold Schmitt-Ulms

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Part IV: Ligand Discovery

17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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18. Working with Small Molecules – Preparing and Storing Stock Solutions and Determination of KineticSolubility

Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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Amy Hye Won Jeon, Gerold Schmitt-Ulms

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17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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Paula de Matos, Nico Adams, Janna Hastings, Pablo Moreno, and Christoph Steinbeck

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17. Ligand Discovery Using Small-Molecule Microarrays

Dominick E. Casalena, Dina Wassaf, and Angela N. Koehler

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Andrea Wolf, Satoko Shimamura and Friedrich B.M. Reinhard

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