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Journal or Scientific & Industri al Research Yol.60, January 200 I, j:Jp 1-9
Chemistry and Pharmacology of Rhubarb (Rheum species)- A Review
Santosh K Agarwal *, Sudhir S Singh, Vijai Lakshmi+,
Sushma Verma and Sushi! Kumar
Central Institute of Medicinal and Aromatic Plants (('SIR) , Lucknow 226 015 , India
+Central Drug Research Institute (CSIR), Luck now 226 00 I , India
Various chemica l constituents iso lated from rhubarb (Rheum ) species include anthraquinones, di anthrones, stilbencs. anthocyanins, navonoids, anthragl ycos icles , polyphenols , organic acids, essential oi ls, vitamins, chromenes, chromanone and chromone glycosides . Notable biological ac ti viti es reported rro111 ,, ,l. vari ous parts or the plant and its iso lates arc cath arti c. an tibacteri al, fatal poisOfling, antiviral, anti inllammatory, mollusciudal , anticoagulant , spas molyti c, cytotoxicity. chronic rcn;ll failure, chemiluminescence quencing and an ti -p latelet acti vities.
Introduction
Rheum Linn (family polygonaceae; English name: rhubarb), a large genu s of perennia l, stout he rbs and dis
tributed in the temperate and sub-tropical regions of the
world, chiefly in Asian countries, viz. India (Kashmir, Assam, Sikkim), Nepal , Bhutan, China, Pak istan , Korea, Turkey, Russ ia and Tibe t. Several species of this plant are used in medicine, some for culinary purposes and few others are grown as ornamental plants. There are three main types of rhubarb , viz. the Chinese Rhu
barb, the Indian or Himalayan Rhubarb and the Rhapontic Rhubarb u. Some known s peci es of Rh eum are R. acuminatum (R-1 ), R. alexandrae (R-2), R. altai cum (R-3), R. australe (R-4), R. compactum (R-5), R. coreanum (R-6), R.corodatum (R-7), R. delavayi (R-8), R. emodi (R-9), R. franzenbachii (R- 1 0), R. hotaoense (R-11 ), R. hybridium (R-1 2), R. kialense (R-1 3), R. /hasa ense (R-14), R. maximoviczii (R-15 ), R. moorcro.fiianum (R-16), R. nobile (R-17), /?. offlcina /e (R-1 8), /?. pa/aestinwn (R-19), R. palmatum (R-20), /?. pami/um (R-2 1 ), /?. ribes (R-22), /?. rhabarbamm (R-23), R. rhaponricwn (R-24), (R-13), R. lhasaense (R-14 ), R. maximoviczii (R- 15), /(
moorcrojtianum (R- 16), /?. nobile (R- 17), /?. oJficina /e (R-1 8), R. palaestinum (R- 19), R. pa/matuiii (R-20), /?. pamilwn (R-21 ), R. ribes (R-22) , R. rhabarbarwn (R-23), R. rhaponticum (R-24), R. spiciforme (R-25), R. tanguticum (R-26), R. tataricum (R-27), R. undu/atum
*Author for correspondence
CIMAP Publi cat ion No.: 99-11 R
(R-28) , R. webbianum (R-29), /?. willrockii (R-30) and R. qinlingense (R-31 ). About ten spec ies are found in India.
Indian rhubarb , which is offic ial in Indian Pharmacopoeia, consists of the dried rhizomes of R. enwdi and R. webbian um and other species of Rheum. Rhi zomes and roots of R. moorcroftianum and R. spicifonne are
also reported to be mixed with the drug. The plant grows wild at an altitude of about 4000 to 12000 feet. Rhubarb has been successfully grown in certa in parts of Assam also. Indian rhubarb occurs in sub-cy lindrica l or irregular pieces, ranging from 2 to 20 em in length and 1.5 to 2.0 em in diameter, much shrunken, lig ht in we ight. It is
some what dark in colour, almost odourl ess or somewhat fragrant with a bitte r and astringe nt taste. It contains anthraquinone derivatives and y ie lds about 25-30 per cent o f ~·x trac t solubl e in 50 pe r cent aqueous alcoho l' . In ayurvedic preparations, rhubarb has long been used as an ingredient of purgatives, laxatives and stomachic-tonics ·' · ~. It is hoped that the rev iew would be useful to those work ing in the fi e ld of phytochem ica l invest igations of rhubarb.
Chemistry
A varie ty of const ituents have been iso lated fro m Rheum spec ies and the ir structures e luc idated. They belong to such classes as anthraquin ones, clianthroncs, stilbenes, anthocynin s, rla vo no icl s, an thrag lycosid es, polyphenols, essenti a l oi l. o rganic ac id s, chromcnc,
2 J SCI IND RES VOL.60 JANUA RY 2001
chromanone, chromone glycos ides and vitamins. Analysis of samples of the rhubarb contained (in percent ) ash, 9.3-4.9; protein , 1. 1; fibre, 1.3-0.8; catharti c acid , 3.5-3.2; organi c ac ids, 3.3-2.2 ; and sugar, 1.4- l . l ; respectively' .
Essential Oil.\·
The essential oil s (0.05 per cent) obtained fro m rhi zomes of R. cmodi have charac teri stic odour due to the presence of eugenol and meth yl heptyl k eton e~ . The vo latile oil from rhi zomes of R. polnw lun1 contained I 08 vola til e components of which 27.3 per cent were terpenoid s. The main constituent s were (per cen t): palmitic aciu (22. 5) , paeono l ( 16. 2), a -co paene (9.8), met hyl
stea rate (9.3), 8-cadinene (5.4), and methyl-eugenol (5.4)('.
An 1 h raquinones
Many an thraq uinones have been iso lated from different spec ies of rhu barb. A few of them are physcion , ch rysophanol (c hrysophanic acid or chrysophan) , rhein , emodi n, aloe-emodi n, rh e inal , hydroxy-meth yl an th raq uinone and Jst izin. Out of these anthraqu inone derivatives, most of the rhubarb spec it.:s contain 7 physc ion, chry~oph anol, emodi n, al oe-emodin and rhein as shown in Tab le I. Indian rhubarb (R. cmodi) was found to contain free rhein (0.48) , free chrysophanol (0.50) and glycosidic rhein ( 1.52) and glycos idic chrysophanol (0.4 1 per cen t)1. The antimicrobial acti vity of rhein was al so observed aga inst Escherichia coli, Bacillus suht iles, Micrococcus luleus, Candida albicons. Clost rirliulil pe1ji-ingr'il.\' and Fusofacleriu/11 voriwn 1. We have also isolated a new anthraq uinone deri vative rheinal (0.008 p,cr cent) from the rh izomes of R. cnwdi ·~.
Dianthrones
Rheidin A is a heterodianthrone co mposed of one mole of emodin and one mole of rhein anthrone, obtai ned from 1<. palmatwnx. Similarl y, three other di anthrones sennidin C (one mole of aloe-emodin and one mole of rhcin ), rhci din B (one mole of rhein and one mole of chrysophanol), and rheidi n C (one mole of rhein <llld one mole of physc ion) were isolated from rhubarb roots') .
Organic Acids
Some orga ni c acids obtained from rhu barb spec ies arc: oxalic, pa lmitic, acet ic, succ ini c, lactic, ma li c, cit ric , fumar ic an d form ic (Tab le I). The oxa li c acid content in In dian rhubarb 1 w;:s 1.3-t per ce nt. Mali c ac id w;,L predominant in al l the v<~ri e ti es tested I 0. Yas uhiko
analyzed the rhubarb extract ( I 00 g) whi ch contained acet ic ac id (8 .5 -39.4), formi c ac id (9.'2-37. 1 ), oxa lic acid ( 100-638), malic ac id (305-1200) and c itri c acid (207-340 mg) . Trace amounts of lac tic, succ in ic and fumari c acids were also detected' . Palmitic ac id content was found to be 49.3 1 per cent in the volatile oil I I of R. tangu ticu m.
Stilbelles
Rhubarb had many stilbene derivatives such as
rhapontici n, deox yrhapont ic in , rh apon t ic i n-~-D-g I ucosid , desoxy rhaponti genin , rhapon tigenin, piceatannol, 4'-0-meth y I pice id , rh apon t i ge n in -3'-0-~ -n-gl ucopyranoside, piceatannol-3 '-0- ~-n-g i ucopy ranos idc (Table I). Rhapontin when ad ministered to ca~t rated female rats (dose 600 ~Lg) has been reported to res tore the oestrous cycle. Also, tab lets containing 5 mg rhapontin and 90 mg of hops-ex tract ex hibited an ac tion sim ilar to th at of the fo lli cular honnone in the treatment of amenorrhoea, dysmenotThoea and endomet ritis and in inh ibiting lactation 1.
Flo vonnids
Mainl y two spec ies, R. laWricum and R. \l'il/mckii. of rhubarb gave fl avo no id s such as qu e rce tin , isoguercetin , meratin, rutin (0.32 per ct:nt) and quercetin-3 ,7-g lucoarbinosides 12· 13
. Rutin has been iso lated from the foll owing Rheum spec ies (per cent ): R. cnwdi (0.32), R. officinale ( 1.30), R. rhapol/(icttlll (0.6 1) and N. undulatum (0 . 70 ) 1 ~ . Alyukina ct a/.·1 found that the ground part of rhubarb (R. \l 'illmckii ) conta ined 18 phenolic compounds, seven of which were flav onoids. The maximum amount of flav onoids was in the fl owers and the immature fruit s (6.57 per cent) .. fo ll owed by ripe fruits and buds (4.60 and4 .1 8 per cent, respecti vely).
Anthocycutins
R. rhaponlicun1 and R. tawricum gave anth ocyanin and their deri va ti ves such as cyaniclin-3-g lucoside. cyanidin -3-rutin os ide, chrysanthemin , ;t nd cya nin 1' 1x.
Vi to 111 ins
Vit amin B. vitami n C (ascorbic acid) and vit am in D have been reported from rhubarb·1·1') Its juice proved a fai rl y good source of vitamin C. as tes ted by prophylactic expe riments 1
.
AGGARWAL eta/.: CHEM ISTRY & PH ARMACOLOGY OF RH UBARB 3
Chromene, Chrornanone, and Chromone
Six chromone and chromanone deri vatives211 have been isolated from rhubarb together with two chromone g lucos ides 211 • Th ese are : 2,5-dimeth y l- 7- hyd roxychromene; 2-methyl-5-acetyl-7-hydroxy-chromene; 2-methyl-5-carboxy-methyl- 7 -hydrox y-chromenc; 2-(2'hydroxy l-propy 1-5-methy 1-7 -hydroxychromene; 2- methyl-5-carbox-ymethyl-7-hyd roxy chromanone; Aloesone-7-0- ~-D-gluco-pyranos ide and 2-methyl-5-(2'-oxo-4'-hydroxypentyl)-7-hydroxy c hromone-7-0 - ~ -D
glucopyranos ide.
Polyphenols
A large number of polyphenols isolated from different spec ies of rhubarb include: (-)-epi gallocatechol, ( + )-ga ll ocatec hol, (- )-epigallocatec hol-gall ocatec hol, ( + )-gall ocatec hol ga ll ate, (+)-catechol, (-)epicatechol gallate, catechins, (-)-epicatechol, (- )-epicatechin ga llate, ga llic acid, glucoga llin, kaempferol, 4,8'-bis-3-0-galloyl(-)-epicatechin , 4' ,8-3-0-galloy 1-(-)-epicatechin-( +)-catec hin , d-catec hin , pyroga ll o l, ca rvacro l and 4-methoxyga llic ac id . The ast rin gent principle consists chiefl y of ga llic ac id , present as gl ucoga ll in , together with small amount of tannin and poss ibl y catec hin . Glucoga llin on hydrolysis yielded galli c acid and glucose1.
Anthraglycosides
Anthraglycos ides are found almost in all the species of rhubarb and a few of them were: rheochrys in , aloe-emod i n-8-0-~-D-g l ucos ide, emod i n-6-0-~-D-g l uco
side, rhe in- 8-0-~-D-g lucos i de, rheinos ide A, rheinos ide B, rhein os ide C and rhe in os ide D, sennos ide A and senn os ide B, to rachryso ne- 8-0-~-D-glucopy ra n os id e,
kaempfe ro 1- 3-0-a- L-rh amn os id e, chryo- phan e i n,
chrysop han ol-8-0- ~-D-gl ucos ide, emod in -8-0- ~-D
gluco-pyranos ide, chrysophano i - 1 -0- ~-D-g lucos id e and rh e in-II -O-~-D-g lucos id e2 (Ta ble I).
Pharmacology
Rhubarb is large ly used as purgative in wes tern medicine. It is a good laxative, stomac hic, bitter, tonic and ca tharti c, so it is well fitted for use in simpl e diarrhoea2 It is so safe that it cou ld be given to children as well as old peop le. Its use is prohibited for pati ent s suffering from gou t, rheumati sm, ep il epsy and uri c acid . troubles. Powdered roots arc used fo r cleaning tee th and are also sp rinkled over ul cers for quick healin g.
The drug is used in Ayurveda. ln th e Unan i system, it is a constituents of " [tri fa l Mulayy in", used in ordinary constipati on and other di gesti ve disorders, ''Hab Shabyar" where headache of different intensity are considered clue to a disturbance in the di ges ti ve system, "Haba Shafa", for an obstin ate type of cough and cold and of "Roughan Aq rab", applied on pil es because or the astringent action of Rheum. "Qur Didan" is an anthelmintic' .
Rhubarb forms an important ingredi ent of a \'a riety of medicines. It is an exce ll ent remedy for irrit ation of the bowels, common among child ren durin g teething and in chronic dysentry, du odenal catarrh or catarrh of the biliary ducts with jaundice and in certain sk in diseases2. Fres h rhubarb juice showed anti scorbuti c act ivity3
The plant also possesses antibiotic propcrti es 13 It is used as an antibacteri al aga inst Stapltvlococcus mm' us, Escherichia coli or Brucella obortu.1·~ . Rhubarb extract had a laxative effect due to its content of hyd roxy methyl anthraquinone and tannins-' . It also has cathartic effect3·57 Cooked leaf and stems are harm less in moderate quantities but the leaves may cause fa tal poisonin g~.
Rhubarb is used as an antiviral agent against Voricello zoster virus and He1pe.1· simplex virtt.\Tubello'x . The minimal inhibiti ve closes aga inst Voricc llo z.osrer and HcrJ"Jes simf'J iex vimsrubello G and JR ,
1 were I 00-
11\· J() - -
5000 mg/L. It has also shown antiinflammatory act i v-ityl . Anthraquinones of rhubarb had mollusc icidal acti vit y against the schi stosome vector snail s On colllclunio , Biomphalaria and Bulinw·5~. Chrysophanic acid and cl -catechin obtained from rhubarb had shown prolongati on of prothrombin time which is a measure of coagui<lting activityn w. Chrysophano l had been shown to be
responsible for spasmolytic act ivityli1.
Chemiluminescence quenc in g activity was found to ex ist mainl y in emod in , aloe-emodin and rhein obtained from rhubarbr'2 . It ex hibited moderate cytotox ic ac ti vity against several types or carc in oma cells due to it s co ntent o f c hryso phan o i - 1 -0-~-D-g lu coside. chrysophanein and physcionin ~ 1 . Chinese rhubarb has been found to be va luable in preventing prog ress ion of
chronic renal failure r, .uo-~ _
Anti-platelet aggregation activity of st i I bene dcri \'ali ves from /?. undttlurttnt has a lso bee n obser\'ed. Rhaponti genin and desoxyrh apo nti gen in ha ve shown strong inhi biti on of aggregati on induced by arachid onic acid and co ll agen·11
. The rhubarb stalk fibre is c!Tccti ve
4 J SCI IND RES VOL.60 JANUARY 2001
Table I - List or compou nds iso lated from illerent spec ies or rhubarb
Sl. No. Name of compound
Essential Oils
Eugenol, bp, 255"
2 Meth yl hep tyl ketone
3 Paeonol
4 a -Co paene. bp, 246-25 1"
5
6
7
(aj 0 - 6.3" (c= 1.2 in C HC I1).
Methyl stearate
P-Cadi nene
[a]o-25 1"
Meth yl-eugenol
Anthraquinones
8
9
10
II
12
13
14
15
Physc ion, mp. 2 10"
Chrysophanol or Chrysophan
Chrysophanic ac id , mp, 196"
Aloe-emodin, mp. 223 -224"
Emodin, mp. 256-257"
Hydroxy-methyl anthraqui none
mp, 302"
Rhein, mp, 32 1-322"
Rheinal, mp, 236-238"
Isti zin
Dian thrones
16
17
18
19
Organi
20
2 1
22
Rheidin A
Sennidin C
Rheidin B
Rheidin C
Acids
Oxa lic acid, mp, 101 - 102"
Palmitic acid, mp, 63-64"
Aceti c acid , bp, 11 8"
Name or plant
R-9
R-9
R-20
R-20
R-20
R-20
R-20
Rhubarb, R-9 , R-20. R- 18,
R-26. R- 10. R-22. R- 16. R-31
Rhubarb, R-9, R-20. R- 18.
R-26. R-10. R-22, R- 16.
R-24. R-27
Rhubarb, R-9, R-20. R- 18.
R-26, R-1 0, R-6
Rhubarb, R-9. R-20, R- 18,
R-26, R- 10, R ·24, R- 16. R-27
Rhubarb ,
Rhubarb, R-9, R-20. R- 18.
R-26. R- 1 0. R-22. R-24
R-9
Rhu barb
R-20
Rhubarb
Rhubarb
Rhubarb
Rhu barb
Rhubarb
Re ference~
4
4
6
6
6
6
6
2 1-29
I . 2 1-31
4, 2 1-23. 25-28, 32
4. 2 1-23. 25-3 1
4
1-4.2 1-24.26-28 .
30
2
I , 33
3, 8
3.9
3, 9
3,9
4.5
R-20. R-26
5, 10
- Contd.
AGGARWAL eta /.: C HEMISTRY & PHARMACOLOGY OF RH UBARB
Table I - Li st of compounds isolated from different species of rhubarb - Contd.
23
24
25
26
27
28
Stilbenes
29
30
3 1
32
33
34
35
36
37
38
Succinic ac id , mp, 185- 187"
Lac ti c acid, mp , 53"
[a] 0 - 2.6"
Malic acid, mp , 13 1-1 32"
Citric ac id , mp , !53"
Fumaric acid, mp , 230"
Formic ac id , bp , I 00.5''
Rh aponti cin or Rhapontin
mp, 236-237"dcc
[a] 0 - 59.5" (Acetone)
Deoxyrh aponticin
Rhaponti cin-~-D-g lucoside
mp, 230-231 "
Desoxyrh apontigenin
Rhapont igenin, mp , 185"
Piceatanno l, mp , 23 1-232"
4'-0-Methylpiccid
Piceatanno i-4'-0-
-~ -D-glucopyranoside
Rhaponti genin -3'-0-
-~-D-glucopyranoside
Piceatannol-3'-0-~-D
g lucopyranos ide (Rheumin )
Flavonoids
39 Quercetin, mp, 3 14" dec
40 lsoquercetin , mp, 225-227"
41 Meratin, mp, 180"
42 Rutin , mp, 2 14-2 15" dec
[a] 0 + 13.82" (Ethanol)
43 Quercetin -3,7- glu coarbi nos ides
Anthocyanins
44 Cyanidin-3-g lucoside mp, 205" dec
Rhubarb
Rhubarb
Rhubarb , R-1 2
Rhubarb
Rhubarb
Rhubarb
Rhubarb , R-22, R-24,
R-20, R- 18, R- 11
R-24, R-30
R-9, R-20, R-24
R-28
R-28 , R-1 I
R-2 8, R-30
R-20
R-30
R-28
R-28
R-27, R- 30
R-27, R-30
R-27
Rhubarb, R-27, R-30,
R-30
Rhubarb , R-24
5, 10
5, 10
5, 10
5, 10
10
10
24, 34-37
34,38,39
4,40, 41
42 , 43
2!i , 37 , 42 , 43
28 , 39, 42 , 43
41
39
44
37 , 44
12, 13
12, 13
12
12- 14
!3
15-17
- Contcl.
5
6 J SCI IND RES VOL.60 JANUA RY 2001
Table I - Li st or compounds isol<11cd from di lfero.:n t species of rhubarb - Contd.. 45 Cya nidi n-3- rut inosidc Rhuh:1rb, R-24 15- 17
46
47
Sterols
48
49
Ch rysant hcmin . mp. 205" dec
Cyan in
~-S it os t ero l . mp. 140"
f a iD- 37" (CHCI,J
Daucostcrol
Vitamins
50
5 1
52
Vit amin B
Vit am in C (Asco rbic acid)
mp. 190-92", Ja iD + 4X" (MeO II)
Vitamin D
Chnnncnc, Chromanone and Chmmonc
53 2.5-Dirncthyl-7 -hydroxy- chromcnc
5-1 2-Mcthyl-5-acctyl-7-
hyJroxy-c hromcnc
55 2-Mcthyl-5-carboxy
mcthyl-7 -hydroxy-c hromcnc
56 2-(2'- H ydro~ylpropy l -5-
mct hyl-7 -hydroxyc hromcnc
57 2-Mcth yl-5-carboxymcth yl-
7-hydroxy chromanonc
5X A l ocsonc-7-0-~-D-
gluco pyranosidc
59 2-Mcthyl-5-(2'-nxo-..J'-
hydroxypcnt yl )-7- hydroxy
chromnnc-7 -0- ~ - 1 )-glucopyranosidc
Polyphcnols
60
66
67
(- )-Epigall ocatechul
( +)-Gal locatcchol
(- )-Epigallocatec hol- gal locatcchol
(±)-Gallocat..:cho l-ga II at..:
(+)-Catechol. mp. 212-2 16"
(-)-Epicatcc hol -gallatc
(- )-Epicatcchol
(- )-Epicatcchin-gal latc
R-27
R-27
R- 11
R- 11
Rhu barb
Rh ubarb
Rh ubarb
Rhubarb. R-30
Rhu barb
Rh ubarh
Rhuba rb
Rhubarb
Rhuba rb
Rhubarb
R- 15
R- 15
R- 15
R- 15
R-20. R-27 . R-30. R-15
R-20. R-27
R-27. R- 15
R-20. R-1 X. R-3. R- 1 0. R- 1 3
18
Ill
37
37
4
4, 6. 19
4
20. ]<)
20
20
20
20
20
20
45
-15
45
3. -16-..JX
] . ..J . 9. 27. -17
46,-17
-17 . ..)<)
- Contd.
AGGARWAL eta/.: C HEMISTRY & PHARMACOLOGY OF RHUBARB
Tabl e I - Li st of compounds iso lated from different spec ies or rhubarb - Contd.
68
69
70
71
72
Gallic acid, mp. 235-240"
Glucogall in
Kaempfcro l , mp. 276-278"
4.8'-bi s-3 -0-Ga ll oyl-(- )-epica techi n
4 .8'-3-0 -Gall oyl-(- )-ep ica techi n
-(+)-catechin
73 Pyroga ll o l, mp. 13 1- 1 3:1''
74
75
Carvacrol , bp. 11 8- 122"
4-Methoxygallic acid
Ant hraglycosides
76
77
78
79
84
85
8(i
87
88
Rheochrys in
Aloe-emodi n-8-0-~-D-gl ucosid<:
mp 237-238"
Emod i n-6-0-~- 0 -g lucosidc
Rhcin-8-0-0-D-glucoside
mp, 260-266"
Rhein- I 1 -0-~-D-gluwsi dc
mp. 247-25 1"
Rhcinoside A
Rhci noside 8
Rhcinoside C
Rhcin os idc D
Scnnosidc A. mp. 200-240'' dec.
[u.Jn - 164" (Acetone)
Scnnns idc B. mp. I Hll- 186" dec
fain- 100" (Acclonc•)
St.:nnosidc C. mp. I 1)7"
Scnn os idc D. 1np. 21 ()"
C hrysoph a no 1-8-0- ~~ -
-( 6' -gal loyl)-g luL , 'I') !'<'IH •side
3
R-20. R- 18. R-3
R-20, R- 18, R-7
R-3. R- 10. R- 13
Rhubarb
Rhubarb
Rhubarb
R- 15
R-1 5
R-20
R-28. R-20
R-20
R-13. R-8. R- 30.
R-2 1. R-1 4, R-26. R-20
R-9
Rhubarb. R-2, R- 14.
R-26. R-8, R-20. R- 13
Rhubarb
Rhubarb. R-2 . R-1 4. R-26.
R-8 . R-20. R-1 3. R-2 1. R-3 0
Rhubarb. R-2. R-14 . R-26,
R-8. R-20. R- 13. R-2 1. R-30
R-30. R-14. R-6 . R-26. R-8.
R-2 0. R- 13
R-6 . R-20
Rhubarb
Rhubarb
R-28
3.37.48
3
20
50
50
48
48
51
27.44. 51
27 .51
27. 52
2
52-54
53
52-54
52-54
3.52.54. 55
3. 55
- Contd.
7
8 J SCI IND RES VOL.60 JANUARY 200 1
Table I - Li st o f compounds iso lated from different species or rhubarb - Contd
90 A l oe-cmod in - 1 -0-~- - D-gluco pyra n oside
91 Torachryso ne-8-0-~-
-D-g lucopyranosidc
92 K aempferol-3-0-a-L-rhamnosidc
93 Ch rysophanein. mp. 246"
94 Ch rysoph a no i-8-0-~-D-g lu cosi d c
mp, 245-246"
95 Emod i n -8-0-~-D-glucopy ra nnsi de
mp, 2 10-2 11 "
96 Chrysoph a n oi-1-0-~- D-g l ucos i dc
mp. 248-249"
97 Chrysoph a no i-8-0-~-D-
-(6 '-malonyl)-g lucopyranosidc
98 Physcion-8-0-~-D-glu cosidc
in loweri ng serum cholesterol concentrati ons, especially LDL cholesterol, in hypercholesterolemic men1
''.
Conclusion
Rhubarb possesses wide range of biolog ica l activ ities and have revealed compounds of many chemi cal classes . Ninety eight compounds iso lated from these species include five flavon oids , twelve anthraquinones, ten stilbenes, sixteen polyphenols and twenty-t hree anthrag lycos ides and rest others. It is believed that thi s rev iew would be of immense use for researchers workin g in the fi eld of phytochemica l in vest igat ions.
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